MIR509-1

Summary

MIR509-1 (microRNA 509-1, HGNC:32146) is a microRNA gene on chromosome Xq27.3.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 574514 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32146
Approved symbol	MIR509-1
Name	microRNA 509-1
Location	Xq27.3
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-509, hsa-mir-509-1
Ensembl gene	ENSG00000208000
Ensembl biotype	miRNA
OMIM	300875
Entrez	574514
RNAcentral	URS000075B85A — miRNA, 94 nt, 2 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385265

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385265 — 1 exons

Exon	Start	End
ENSE00001500271	147260532	147260625

Expression profiles

Bgee: expression breadth broad, 47 present calls, max score 83.20.

Top tissues by expression

47 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
mucosa of transverse colon	UBERON:0004991	83.20	gold quality
metanephros cortex	UBERON:0010533	79.56	gold quality
blood	UBERON:0000178	75.26	gold quality
gall bladder	UBERON:0002110	75.22	gold quality
right lobe of liver	UBERON:0001114	75.10	gold quality
gastrocnemius	UBERON:0001388	74.86	gold quality
liver	UBERON:0002107	74.47	gold quality
skin of abdomen	UBERON:0001416	73.18	gold quality
adult mammalian kidney	UBERON:0000082	73.15	gold quality
ascending aorta	UBERON:0001496	73.13	gold quality
colon	UBERON:0001155	73.09	gold quality
transverse colon	UBERON:0001157	72.97	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	72.72	gold quality
intestine	UBERON:0000160	71.75	gold quality
endometrium	UBERON:0001295	71.14	gold quality
lung	UBERON:0002048	70.91	gold quality
substantia nigra	UBERON:0002038	70.88	gold quality
body of pancreas	UBERON:0001150	70.02	gold quality
body of stomach	UBERON:0001161	69.38	gold quality
esophagus mucosa	UBERON:0002469	69.18	gold quality
small intestine Peyer’s patch	UBERON:0003454	68.75	gold quality
urinary bladder	UBERON:0001255	68.09	gold quality
amygdala	UBERON:0001876	67.91	gold quality
muscle layer of sigmoid colon	UBERON:0035805	67.70	gold quality
lower esophagus muscularis layer	UBERON:0035833	67.08	gold quality
right frontal lobe	UBERON:0002810	67.06	gold quality
Ammon’s horn	UBERON:0001954	66.81	gold quality
myometrium	UBERON:0001296	66.54	gold quality
tibial artery	UBERON:0007610	66.39	gold quality
skin of leg	UBERON:0001511	66.10	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.33

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 14)

• miR-509-5p plays an important role in renal cell carcinoma by inhibiting cell proliferation and migration and by promoting cell apoptosis. (PMID:23619562)
• The expression of miR-509-5p in HeLa and QGY-7703 cells repressed the expression of Mdm2, subsequently enhancing its p53-activating effects. (PMID:25144722)
• Mir509 has a critical role in brain metastasis of breast cancer by modulating the RhoC-TNF-alpa network. (PMID:25659578)
• The miR-509-5p could bind to the 3’ untranslational regions of FOXM1 mRNA. (PMID:27035590)
• Our findings strongly demonstrate that miR-509 functions as tumor suppressor in TNBC BC cells through, at least partially, suppressing the secretion of TNF-alpha. (PMID:27656833)
• The present study revealed the tumor suppressive role of miR-509-5p in non-small cell lung cancer (NSCLC) by targeting YWHAG, suggesting that miR-509-5p/YWHAG axis might be considered as a novel and potential target for clinical diagnosis and therapeutics of NSCLC. (PMID:27894843)
• Results suggest that microRNA miRNA-509-5p negatively regulates c-mdm2 proto-oncogene protein (MDM2) expression via targeting the 3’ untranslated region (3’-UTR) of genes. (PMID:28252164)
• miR-509-5p was associated with worse overall survival in patients with pancreatic cancer and was identified as an independently selected predictor of mortality. Our findings suggest that miR-509-5p and miR-1243 might be novel chemotherapeutic targets and serve as biomarkers in pancreatic cancer. (PMID:28638102)
• MiR-509-5p exerted tumor-suppressive effects on breast cancer progression and metastasis via targeting SOD2 in vitro (PMID:28925482)
• MiR-509-3p could sensitize ovarian cancer cells to cisplatin treatment. (PMID:29173002)
• Through conducting multivariate analysis in the training test, it was identified that the high expression of hsa-miR-509 and hsa-miR-542 were independent poor prognostic factors, whereas that of hsa-miR-146a and hsa-miR-3667 had a trend to be favorable factors. A 4-miRNA signature was constructed by these miRNAs considering the weight of each. (PMID:30242879)
• MiR-509-3-5p inhibits colon cancer malignancy by suppressing GTSE1. (PMID:34284144)
• Expression of miR-187 and miR-509-3p in serum of primary hepatocellular carcinoma patients and its evaluation of prognosis. (PMID:34564990)
• miR-509-5p promotes colorectal cancer cell ferroptosis by targeting SLC7A11. (PMID:37229918)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir509	ENSMUSG00000106233

Paralogs (9): MIR506 (ENSG00000207731), MIR514A2 (ENSG00000207866), MIR514A3 (ENSG00000207867), MIR514A1 (ENSG00000207868), MIR513B (ENSG00000207871), MIR507 (ENSG00000207969), MIR513A2 (ENSG00000207984), MIR509-3 (ENSG00000212014), MIR514B (ENSG00000252583)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.