MIR514A3

Summary

MIR514A3 (microRNA 514a-3, HGNC:32150) is a microRNA gene on chromosome Xq27.3.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 574518 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32150
Approved symbol	MIR514A3
Name	microRNA 514a-3
Location	Xq27.3
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-514-3
Ensembl gene	ENSG00000207867
Ensembl biotype	miRNA
Entrez	574518
RNAcentral	URS000075B438 — miRNA, 88 nt, 3 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385132

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385132 — 1 exons

Exon	Start	End
ENSE00001808154	147284641	147284728

Expression profiles

Bgee: expression breadth broad, 16 present calls, max score 79.10.

Top tissues by expression

16 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	79.10	gold quality
blood	UBERON:0000178	74.55	gold quality
ascending aorta	UBERON:0001496	65.63	gold quality
body of pancreas	UBERON:0001150	63.73	gold quality
muscle layer of sigmoid colon	UBERON:0035805	63.67	gold quality
body of stomach	UBERON:0001161	63.08	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	61.10	gold quality
adult mammalian kidney	UBERON:0000082	57.64	gold quality
tibial nerve	UBERON:0001323	57.49	gold quality
right frontal lobe	UBERON:0002810	56.55	gold quality
skin of leg	UBERON:0001511	55.48	gold quality
nucleus accumbens	UBERON:0001882	55.40	gold quality
prostate gland	UBERON:0002367	55.35	gold quality
pituitary gland	UBERON:0000007	51.15	gold quality
corpus callosum	UBERON:0002336	44.19	silver quality
left testis	UBERON:0004533	27.26	silver quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.49

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 5)

• miR-514a has the ability to bind to NF1 and inhibit its translation, and miR-514a expression is involved in modulating cell proliferation rates along with BRAFi (PLX4032) sensitivity in melanoma. (PMID:25980496)
• Loss of miR-514a-3p expression in testicular germ cell tumor increases PEG3 expression that recruits TRAF2 and activates the NF-kappa B pathway, which protects germ cells from apoptosis. (PMID:28471449)
• miR-514a-3p: a novel SHP-2 regulatory miRNA that modulates human cytotrophoblast proliferation. (PMID:34792485)
• LncRNA OSTM1-AS1 acts as an oncogenic factor in Wilms’ tumor by regulating the miR-514a-3p/MELK axis. (PMID:35946509)
• Human Oral Mucosa Stem Cells Increase Survival of Neurons Affected by In Vitro Anoxia and Improve Recovery of Mice Affected by Stroke Through Time-limited Secretion of miR-514A-3p. (PMID:36083390)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir509	ENSMUSG00000106233

Paralogs (9): MIR506 (ENSG00000207731), MIR514A2 (ENSG00000207866), MIR514A1 (ENSG00000207868), MIR513B (ENSG00000207871), MIR507 (ENSG00000207969), MIR513A2 (ENSG00000207984), MIR509-1 (ENSG00000208000), MIR509-3 (ENSG00000212014), MIR514B (ENSG00000252583)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.