MIR516B1

Summary

MIR516B1 (microRNA 516b-1, HGNC:32122) is a microRNA gene on chromosome 19q13.42.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 574490 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32122
Approved symbol	MIR516B1
Name	microRNA 516b-1
Location	19q13.42
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-516-4, hsa-mir-516b-1
Ensembl gene	ENSG00000207946
Ensembl biotype	miRNA
OMIM	620619
Entrez	574490
RNAcentral	URS00006ACABB — miRNA, 90 nt, 13 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385211

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385211 — 1 exons

Exon	Start	End
ENSE00001807135	53736845	53736934

Expression profiles

Bgee: expression breadth broad, 11 present calls, max score 85.00.

Top tissues by expression

11 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
muscle of leg	UBERON:0001383	85.00	gold quality
blood	UBERON:0000178	75.23	gold quality
right lobe of liver	UBERON:0001114	71.49	gold quality
lower esophagus muscularis layer	UBERON:0035833	71.03	gold quality
omental fat pad	UBERON:0010414	69.35	gold quality
body of stomach	UBERON:0001161	66.35	gold quality
transverse colon	UBERON:0001157	65.38	gold quality
left ovary	UBERON:0002119	64.68	gold quality
endocervix	UBERON:0000458	63.70	gold quality
esophagus mucosa	UBERON:0002469	63.65	gold quality
metanephros cortex	UBERON:0010533	60.32	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.20

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 10)

• miR-516b functions as a tumor suppressor by directly modulating CCNG1 expression in esophageal squamous carcinoma cells. (PMID:30119241)
• Circ_0079593 facilitates proliferation, metastasis, glucose metabolism and inhibits apoptosis in melanoma by regulating the miR-516b/GRM3 axis. (PMID:32839935)
• Long noncoding RNA FEZF1AS1 facilitates nonsmall cell lung cancer progression via the ITGA11/miR516b5p axis. (PMID:33174014)
• LINC01123 enhances osteosarcoma cell growth by activating the Hedgehog pathway via the miR-516b-5p/Gli1 axis. (PMID:33837611)
• Late/post-term decidual basalis-derived mesenchymal stem/stromal cells show evidence of advanced ageing and downregulation of microRNA-516b-5p. (PMID:33975264)
• Ginsenoside Rg3 inhibits osteosarcoma progression by reducing circ_0003074 expression in a miR-516b-5p/KPNA4-dependent manner. (PMID:34930332)
• LINC02362 attenuates hepatocellular carcinoma progression through the miR-516b-5p/SOSC2 axis. (PMID:34990401)
• The hsa-miR-516a-5p and hsa-miR-516b-5p microRNAs reduce the migration and invasion on T98G glioblastoma cell line. (PMID:36410106)
• LncRNA ARAP1-AS1 targets miR-516b-5p/PDE5A axis to facilitate the progression of thyroid cancer. (PMID:36730555)
• Circ_CHMP5 aggravates oxidized low-density lipoprotein-induced damage to human umbilical vein endothelial cells through miR-516b-5p/TGFbetaR2 axis. (PMID:37212088)

Cross-species orthologs

0 orthologs

Paralogs (24): MIR520E (ENSG00000207599), MIR515-2 (ENSG00000207615), MIR515-1 (ENSG00000207616), MIR516A2 (ENSG00000207620), MIR526A1 (ENSG00000207629), MIR521-1 (ENSG00000207634), MIR518F (ENSG00000207706), MIR525 (ENSG00000207711), MIR520B (ENSG00000207722), MIR517A (ENSG00000207734), MIR520C (ENSG00000207738), MIR516A1 (ENSG00000207767), MIR519C (ENSG00000207788), MIR519B (ENSG00000207825), MIR517B (ENSG00000207837), MIR518B (ENSG00000207862), MIR518E (ENSG00000207987), MIR524 (ENSG00000283289), MIR518D (ENSG00000283330), MIR523 (ENSG00000283455), MIR518C (ENSG00000283490), MIR520F (ENSG00000283540), MIR522 (ENSG00000283685), MIR519A2 (ENSG00000284362)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.