MIR519D

Summary

MIR519D (microRNA 519d, HGNC:32112) is a microRNA gene on chromosome 19q13.42.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 574480 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32112
Approved symbol	MIR519D
Name	microRNA 519d
Location	19q13.42
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-519d
Ensembl gene	ENSG00000207981
Ensembl biotype	miRNA
OMIM	614247
Entrez	574480
RNAcentral	URS00006656A0 — miRNA, 88 nt, 2 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385246

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385246 — 1 exons

Exon	Start	End
ENSE00001500252	53713347	53713434

Expression profiles

Bgee: expression breadth broad, 27 present calls, max score 82.54.

Top tissues by expression

27 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	82.54	gold quality
ascending aorta	UBERON:0001496	72.40	gold quality
colon	UBERON:0001155	72.12	gold quality
tibial artery	UBERON:0007610	72.11	gold quality
thoracic aorta	UBERON:0001515	71.90	gold quality
monocyte	CL:0000576	71.30	gold quality
blood	UBERON:0000178	70.40	gold quality
right lobe of liver	UBERON:0001114	69.02	gold quality
esophagus mucosa	UBERON:0002469	67.68	gold quality
lower esophagus muscularis layer	UBERON:0035833	67.63	gold quality
omental fat pad	UBERON:0010414	67.25	gold quality
apex of heart	UBERON:0002098	66.27	gold quality
cerebellar hemisphere	UBERON:0002245	66.15	gold quality
skin of abdomen	UBERON:0001416	65.68	gold quality
left ovary	UBERON:0002119	65.08	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	64.53	gold quality
body of uterus	UBERON:0009853	64.24	gold quality
renal glomerulus	UBERON:0000074	63.92	gold quality
spleen	UBERON:0002106	63.30	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	63.15	gold quality
tibial nerve	UBERON:0001323	61.17	gold quality
left lobe of thyroid gland	UBERON:0001120	60.32	gold quality
pituitary gland	UBERON:0000007	58.73	gold quality
lung	UBERON:0002048	58.10	gold quality
right frontal lobe	UBERON:0002810	57.47	gold quality
placenta	UBERON:0001987	53.75	gold quality
left testis	UBERON:0004533	49.33	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.33

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 30)

• findings indicate that miR-519d targets the MKi67 transcript and suppresses HCC cell growth, suggesting that miR-519d has a tumor suppressive role in human HCC pathogenesis (PMID:21524841)
• In hepatocellular carcinoma miR-519d is up-regulated by p53 and DNA hypomethylation and targets CDKN1A/p21, PTEN, AKT3 and TIMP2. (PMID:22262409)
• This study provides further data for the identification of a miRNA profile for glioblastoma and suggests that different-grade neoplasia could be characterized by different expression of specific miRNAs. (PMID:24412053)
• indirectly regulates the extravillous trophoblast invasive phenotype by targeting CXCL6, NR4A2 and FOXL2 transcripts through a 3’UTR miRNA-responsive element (PMID:25211593)
• resistin promotes chondrosarcoma metastasis and MMP-2 expression through activation of the AMPK/p38 signaling pathway and down-regulation of miR-519d expression (PMID:25404641)
• MiR-519d-3p is upregulated in the placenta of patients with preeclampsia and downregulated in a trophoblastic tumor cell line. (PMID:25803859)
• Study showed low expression level of miR-519d associated with TNM staging and metastasis of breast cancer, and suggested it as a tumor suppressor with role in breast cancer cell proliferation and invasion by downregulating STAT3. (PMID:26238950)
• In this review, we focus on the function of miR-519d in placental trophoblasts, where miR-519d regulates cell migration and invasion, and its aberrant expression is associated with preeclampsia. (PMID:27871470)
• E2F transcription factor 1 (E2F1) downregulated micrRNA miR-519d directly and miR-519d downregulated Ras homolog gene family member C (RhoC) directly. (PMID:28146423)
• Low mir519d expression is associated with cisplatin-resistance in breast cancer. (PMID:28423543)
• A significant functional role for miR-519d in determining EphA4 expression and melanoma progression. (PMID:29093007)
• findings suggest that miR-519d-3p regulates the LIMK1/CFL1 pathway in breast cancer (PMID:29188531)
• Results show that miR-519d expression was significantly downregulated in hypertrophic scar tissues and fibroblasts. (PMID:29428666)
• miR-519d overexpression dramatically suppressed MCF-7 cells proliferation, migration and invasion. While downregulation of miR-519d by miR-519d inhibitor substantially increased MCF-7 cell carcinogenesis. (PMID:29483840)
• Results showed that miR-519d-3p was downregulated in gastric cancer (GC) tissues and was associated with the clinical stage and lymph node metastasis of GC tissues. The study demonstrated that miR-519d-3p functions as a tumor suppressor by targeting and downregulating the expression of BCL6 in GC cells. (PMID:29510377)
• Overexpression of miR-519d-3p can inhibit the proliferation of prostate cancer cells. The possible mechanism is that miR-519d-3p inhibits the expression of TRAF4. (PMID:29595452)
• MiR519D inhibits the expression of CCND1 and plays a role in alleviating 5-fluorouracil resistance in colorectal cancer cells. (PMID:29771440)
• our results concluded that CASC9 promotes STAT3 expression via sponging miR-519d, in return, STAT3 activate CASC9 transcription, forming a positive feedback loop of CASC9/miR-519d/STAT3. The novel finding provides a potential therapeutic target for glioma. (PMID:30270508)
• Long noncoding RNA PVT1 promotes laryngeal squamous cell carcinoma development by acting as a molecular sponge to regulate miR-519d-3p. (PMID:30304557)
• It was noted that BAMBI is a target of miR519d3p and miR519d3p promotes transforming growth factor beta/mothers against decapentaplegic homolog 9mediated postoperative epidural scar formation via suppression of BAMBI. (PMID:31485673)
• Cyclin D1 (CCND1) was predicted and confirmed as the direct target genes of miR-519d-3p using luciferase report assay. In addition, knockdown of CCND1 imitated the suppressive effects of miR-519d-3p on cell proliferation and cell cycle progression (PMID:31661371)
• Down-regulation of lncRNA BLACAT1 inhibits ovarian cancer progression by suppressing the Wnt/beta-catenin signaling pathway via regulating miR-519d-3p. (PMID:32095930)
• circ_0007385 served as competing endogenous RNA for miR-519d-3p to suppress malignant behaviors and cisplatin resistance of non-small cell lung cancer cells. (PMID:32602212)
• Circular noncoding RNA circMBOAT2 is a novel tumor marker and regulates proliferation/migration by sponging miR-519d-3p in colorectal cancer. (PMID:32796815)
• H19 promotes aerobic glycolysis, proliferation, and immune escape of gastric cancer cells through the microRNA-519d-3p/lactate dehydrogenase A axis. (PMID:33756038)
• miR519d3p/HIF2alpha axis increases the chemosensitivity of human cervical cancer cells to cisplatin via inactivation of PI3K/AKT signaling. (PMID:33760204)
• Downregulation of miR-519d-3p is Associated with Poor Outcomes and Facilitates Tumor Progression in Papillary Thyroid Cancer by Regulating FOXQ1. (PMID:34496413)
• Transcription factor p53-mediated activation of miR-519d-3p and downregulation of E2F1 attenuates prostate cancer growth and metastasis. (PMID:34799723)
• Identification and validation of aging-related genes in COPD based on bioinformatics analysis. (PMID:35609226)
• Impact of miR-519d on the Biological Activity of Non-Small Cell Lung Cancer Cells and Its Mechanism. (PMID:37820678)

Cross-species orthologs

0 orthologs

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.