MIR520D

Summary

MIR520D (microRNA 520d, HGNC:32114) is a microRNA gene on chromosome 19q13.42.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 574482 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32114
Approved symbol	MIR520D
Name	microRNA 520d
Location	19q13.42
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-520d
Ensembl gene	ENSG00000207735
Ensembl biotype	miRNA
OMIM	620612
Entrez	574482
RNAcentral	URS00006A9A0F — miRNA, 87 nt, 2 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385002

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385002 — 1 exons

Exon	Start	End
ENSE00001807727	53720096	53720182

Expression profiles

Bgee: expression breadth broad, 20 present calls, max score 75.92.

Top tissues by expression

20 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
placenta	UBERON:0001987	75.92	gold quality
gastrocnemius	UBERON:0001388	75.10	gold quality
Brodmann (1909) area 9	UBERON:0013540	72.84	gold quality
body of stomach	UBERON:0001161	71.20	gold quality
ascending aorta	UBERON:0001496	69.99	gold quality
body of pancreas	UBERON:0001150	69.28	gold quality
left adrenal gland	UBERON:0001234	67.68	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	67.23	gold quality
skin of abdomen	UBERON:0001416	67.21	gold quality
lower esophagus muscularis layer	UBERON:0035833	67.11	gold quality
blood	UBERON:0000178	66.63	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	66.35	gold quality
esophagus mucosa	UBERON:0002469	63.74	gold quality
transverse colon	UBERON:0001157	63.59	gold quality
spleen	UBERON:0002106	61.51	gold quality
ectocervix	UBERON:0012249	61.48	gold quality
metanephros cortex	UBERON:0010533	59.97	gold quality
tibial nerve	UBERON:0001323	58.52	gold quality
left testis	UBERON:0004533	42.89	gold quality
right testis	UBERON:0004534	40.61	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.49

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 13)

• Up-regulation of let-520D is associated with periodontitis. (PMID:22128589)
• MicroRNA 520d-3p inhibits gastric cancer cell proliferation, migration, and invasion by downregulating EphA2 expression. (PMID:25063221)
• miR-520d-5p targeting of TWIST1 resulted in reduced expression of the prominent metastamiR miR-10b (PMID:25426550)
• The mature form of MIR520D is significantly over-expressed in low-grade brain astrocytoma samples compared to high-grade and control tissue samples. (PMID:27900675)
• Lowly expressed microRNA-520d-3p was observed in osteosarcoma; overexpression of microRNA-520d-3p can target Akt1 thus inhibiting proliferation of osteosarcoma cells. (PMID:29762834)
• MIR520D serum levels were higher in Parkinson’s disease patients compared to controls. (PMID:30243884)
• MIR-520d-3p up-regulation resulted in marked inhibition of cell growth, migration and invasion in hepatocellular carcinoma tumor cells. (PMID:30551417)
• miR-520d-5p can reduce the mutations in hepatoma cancer cells (PMID:31202279)
• MiR-520d-5p suppressed the proliferation, invasion, and migration of cervical cancer cells via targeting PTK2 (PMID:32198053)
• MicroRNA-520d-3p alleviates hypoxia/reoxygenation-induced damage in human cardiomyocytes by targeting ATG-12. (PMID:33389611)
• MicroRNA-520d-3p suppresses melanoma cells proliferation by inhibiting the anti-silencing function 1B histone chaperone. (PMID:34872448)
• HOXA cluster antisense RNA 2 elevates KIAA1522 expression through microRNA-520d-3p and insulin like growth factor 2 mRNA binding protein 3 to promote the growth of vascular smooth muscle cells in thoracic aortic aneurysm. (PMID:35730141)
• Early Leishmania infectivity depends on miR-372/373/520d family-mediated reprogramming of polyamines metabolism in THP-1-derived macrophages. (PMID:38200138)

Cross-species orthologs

0 orthologs

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.