MIR548K

Summary

MIR548K (microRNA 548k, HGNC:35285) is a microRNA gene on chromosome 11q13.3.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 100313770 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:35285
Approved symbol	MIR548K
Name	microRNA 548k
Location	11q13.3
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-548k
Ensembl gene	ENSG00000221333
Ensembl biotype	miRNA
Entrez	100313770
RNAcentral	URS000075D3AD — ncRNA, 116 nt, 3 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000408406

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000408406 — 1 exons

Exon	Start	End
ENSE00001565041	70283955	70284070

Expression profiles

Bgee: expression breadth broad, 76 present calls, max score 82.85.

Top tissues by expression

76 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
prefrontal cortex	UBERON:0000451	82.85	gold quality
calcaneal tendon	UBERON:0003701	80.19	gold quality
monocyte	CL:0000576	77.16	gold quality
blood	UBERON:0000178	76.72	gold quality
stomach	UBERON:0000945	75.79	gold quality
corpus callosum	UBERON:0002336	75.24	gold quality
endometrium	UBERON:0001295	73.99	gold quality
adrenal tissue	UBERON:0018303	72.58	gold quality
gastrocnemius	UBERON:0001388	72.23	gold quality
liver	UBERON:0002107	72.05	gold quality
tibial artery	UBERON:0007610	71.99	gold quality
myometrium	UBERON:0001296	71.50	gold quality
bone marrow	UBERON:0002371	71.26	gold quality
kidney	UBERON:0002113	71.00	gold quality
body of stomach	UBERON:0001161	70.76	gold quality
body of pancreas	UBERON:0001150	70.21	gold quality
colon	UBERON:0001155	69.70	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	69.70	gold quality
body of uterus	UBERON:0009853	69.58	gold quality
heart	UBERON:0000948	69.50	gold quality
right lobe of liver	UBERON:0001114	69.14	gold quality
adult mammalian kidney	UBERON:0000082	69.10	gold quality
adenohypophysis	UBERON:0002196	68.83	gold quality
heart left ventricle	UBERON:0002084	68.59	gold quality
transverse colon	UBERON:0001157	68.35	gold quality
intestine	UBERON:0000160	68.25	gold quality
esophagus mucosa	UBERON:0002469	67.62	gold quality
putamen	UBERON:0001874	67.53	gold quality
omental fat pad	UBERON:0010414	67.46	gold quality
fundus of stomach	UBERON:0001160	67.37	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.65

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 5)

• miR-548k regulates the mRNA expression of its target gene CXCL13 in the thymus of myasthenia gravis patients with thymus hyperplasia and plays an important role in myasthenia gravis with thymus hyperplasia pathogenesis (PMID:29661539)
• Study identified miR-548k as a promising biomarker for prognosis of esophageal squamous cell carcinoma (ESCC). MiR-548k was significantly upregulated in ESCC and associated with poor prognosis. Data further characterized SCNAs in ESCC and highlighted the crucial role of miR-548k in promoting lymphatic metastasis (PMID:30131072)
• MicroRNA-548k upregulates a spliced variant of human CD2-associated protein by targeting its promoter. (PMID:32608582)
• MiR-548k suppresses apoptosis in breast cancer cells by affecting PTEN/PI3K/AKT signaling pathway. (PMID:36309967)
• ORAOV1, CCND1, and MIR548K Are the Driver Oncogenes of the 11q13 Amplicon in Squamous Cell Carcinoma. (PMID:37930255)

Cross-species orthologs

0 orthologs

Paralogs (25): MIR548AA1 (ENSG00000207704), MIR579 (ENSG00000207956), MIR548P (ENSG00000221263), MIR548F3 (ENSG00000221436), MIR548F1 (ENSG00000221594), MIR548N (ENSG00000221669), MIR548H3 (ENSG00000221698), MIR548AN (ENSG00000263515), MIR548X2 (ENSG00000263581), MIR548AX (ENSG00000263652), MIR548AS (ENSG00000263741), MIR548AV (ENSG00000263750), MIR548S (ENSG00000265056), MIR548AQ (ENSG00000265470), MIR548AG2 (ENSG00000265617), MIR548AJ1 (ENSG00000265669), MIR548AG1 (ENSG00000265829), MIR548X (ENSG00000265841), MIR548Z (ENSG00000266016), MIR548H5 (ENSG00000266807), MIR548AE1 (ENSG00000266808), MIR548O2 (ENSG00000275453), MIR548AY (ENSG00000276147), MIR548AZ (ENSG00000276479), MIR548AH (ENSG00000283682)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.