MIR616

Summary

MIR616 (microRNA 616, HGNC:32872) is a microRNA gene on chromosome 12q13.3.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 693201 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32872
Approved symbol	MIR616
Name	microRNA 616
Location	12q13.3
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-616
Ensembl gene	ENSG00000208028
Ensembl biotype	miRNA
OMIM	614489
Entrez	693201
RNAcentral	URS00006DC233 — miRNA, 97 nt, 1 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385293

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385293 — 1 exons

Exon	Start	End
ENSE00001500299	57519163	57519259

Expression profiles

Bgee: expression breadth ubiquitous, 116 present calls, max score 97.37.

Top tissues by expression

119 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
bone marrow	UBERON:0002371	97.37	gold quality
granulocyte	CL:0000094	96.29	gold quality
sural nerve	UBERON:0015488	95.60	gold quality
monocyte	CL:0000576	94.25	gold quality
mucosa of stomach	UBERON:0001199	93.59	gold quality
endometrium	UBERON:0001295	91.25	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	89.81	gold quality
left uterine tube	UBERON:0001303	88.71	gold quality
skin of abdomen	UBERON:0001416	88.28	gold quality
right adrenal gland cortex	UBERON:0035827	87.90	gold quality
zone of skin	UBERON:0000014	87.23	gold quality
adrenal tissue	UBERON:0018303	87.11	gold quality
blood	UBERON:0000178	86.86	gold quality
left adrenal gland cortex	UBERON:0035825	86.76	gold quality
skin of leg	UBERON:0001511	86.67	gold quality
body of stomach	UBERON:0001161	86.66	gold quality
left adrenal gland	UBERON:0001234	86.48	gold quality
apex of heart	UBERON:0002098	86.40	gold quality
adrenal gland	UBERON:0002369	86.26	gold quality
right adrenal gland	UBERON:0001233	86.11	gold quality
omental fat pad	UBERON:0010414	86.02	gold quality
stomach	UBERON:0000945	85.98	gold quality
thoracic mammary gland	UBERON:0005200	85.89	gold quality
pituitary gland	UBERON:0000007	85.70	gold quality
adult mammalian kidney	UBERON:0000082	85.50	gold quality
adipose tissue	UBERON:0001013	85.41	gold quality
kidney	UBERON:0002113	85.39	gold quality
metanephros cortex	UBERON:0010533	85.34	gold quality
adenohypophysis	UBERON:0002196	85.33	gold quality
left lobe of thyroid gland	UBERON:0001120	85.31	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	5.86

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 18)

• Findings define a functional involvement for miR-616 and TFPI-2 in the development and maintenance of androgen-independent prostate cancer. (PMID:21224345)
• Results from 3’untranslated region reporter assays showed that PON1 is a direct target gene of miR-616. (PMID:23497787)
• Study shows that miR-616 is up-regulated in hepatocellular carcinoma (HCC) tissues, and is associated with recurrence and metastasis. miR-616 contributes to the metastasis and epithelial-mesenchymal transition of HCC by inhibiting the expression of PTEN. (PMID:26499912)
• Target gene PON 1 of hsa-miRNA-616 SNPs is associated with the reduced incidence risk of pCAD. (PMID:27615006)
• miR-616 acted as a tumor promoter in glioma, and its oncogenic roles were involved in the regulation of SOX7 and Wnt/beta-catenin signaling. (PMID:29271996)
• Study demonstrated that the level of miR616 was increased in ovarian cancer (OC) tissues. A high miR616 level was associated with poor tumor differentiation, advanced TNM stage and poor prognosis of OC patients. Also, miR616 promoted the progression of OC by enhancing cell migration, invasion and EMT. (PMID:29658596)
• miR-616-3p-PTEN signaling axis might be a potential therapeutic target for gastric cancer. (PMID:29777710)
• MiR-616-3p binds specifically to the 3’-UTR region of TFPI2 mRNA. (PMID:30028057)
• Mechanistic investigations demonstrated that TUSC7 can interact with miR-616 and decrease its expression, thereby upregulating the expression of miR-616’s targets SOCS4 (SOCS5). (PMID:31200002)
• miR-576, miR-616 and miR-133a-2 are three newly discovered biomarkers of renal cell carcinoma in this study. (PMID:31350524)
• MiR-616 promotes the progression of pancreatic carcinoma by targeting OXR1. (PMID:31502810)
• MicroRNAs Affect Complement Regulator Expression and Mitochondrial Activity to Modulate Cell Resistance to Complement-Dependent Cytotoxicity. (PMID:31537542)
• Circular RNA hsa_circ_0001178 facilitates the invasion and metastasis of colorectal cancer through upregulating ZEB1 via sponging multiple miRNAs. (PMID:31747371)
• LOC285194 inhibits proliferation of human keratinocytes through regulating miR-616/GATA3 pathway. (PMID:32439362)
• Circ_DCAF6 potentiates cell stemness and growth in breast cancer through GLI1-Hedgehog pathway. (PMID:32668286)
• circSLC25A13 acts as a ceRNA to regulate AML progression via miR-616-3p/ADCY2 axis. (PMID:37493101)
• UPR-Induced miR-616 Inhibits Human Breast Cancer Cell Growth and Migration by Targeting c-MYC. (PMID:37685841)
• miR-616-3p alleviates inflammatory response by targeting C-X-C motif chemokine ligand 5. (PMID:38042032)

Cross-species orthologs

0 orthologs

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.