MIR654

Summary

MIR654 (microRNA 654, HGNC:32910) is a microRNA gene on chromosome 14q32.31.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 724024 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32910
Approved symbol	MIR654
Name	microRNA 654
Location	14q32.31
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-654
Ensembl gene	ENSG00000207934
Ensembl biotype	miRNA
Entrez	724024
RNAcentral	URS000075B509 — miRNA, 81 nt, 17 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385199

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385199 — 1 exons

Exon	Start	End
ENSE00001500205	101040219	101040299

Expression profiles

Bgee: expression breadth broad, 53 present calls, max score 97.62.

Top tissues by expression

53 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adrenal tissue	UBERON:0018303	97.62	gold quality
placenta	UBERON:0001987	86.96	gold quality
stomach	UBERON:0000945	86.08	gold quality
lung	UBERON:0002048	84.83	gold quality
kidney	UBERON:0002113	81.08	gold quality
heart	UBERON:0000948	79.72	gold quality
intestine	UBERON:0000160	79.60	gold quality
adrenal gland	UBERON:0002369	76.24	gold quality
calcaneal tendon	UBERON:0003701	76.08	gold quality
liver	UBERON:0002107	75.26	gold quality
colon	UBERON:0001155	74.18	gold quality
gastrocnemius	UBERON:0001388	72.33	gold quality
islet of Langerhans	UBERON:0000006	71.99	gold quality
substantia nigra	UBERON:0002038	69.27	gold quality
right adrenal gland	UBERON:0001233	68.91	gold quality
prefrontal cortex	UBERON:0000451	68.69	gold quality
skin of abdomen	UBERON:0001416	68.61	gold quality
body of stomach	UBERON:0001161	68.50	gold quality
endometrium	UBERON:0001295	68.39	gold quality
anterior cingulate cortex	UBERON:0009835	68.23	gold quality
Ammon’s horn	UBERON:0001954	68.06	gold quality
esophagus	UBERON:0001043	67.42	gold quality
lower esophagus muscularis layer	UBERON:0035833	67.42	gold quality
left adrenal gland cortex	UBERON:0035825	67.10	gold quality
left adrenal gland	UBERON:0001234	66.70	gold quality
omental fat pad	UBERON:0010414	66.11	gold quality
adult mammalian kidney	UBERON:0000082	65.28	gold quality
tibial artery	UBERON:0007610	65.07	gold quality
cerebral cortex	UBERON:0000956	65.05	gold quality
muscle layer of sigmoid colon	UBERON:0035805	64.71	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.16

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 28)

• Mir-654-3p, mapped to the 14q32.31 locus, regulates proliferation, apoptosis, migration and invasion in metastatic prostate cancer cells. (PMID:24166498)
• Report down-regulation of 14q32-encoded miRNA-654-3p in papillary thyroid carcinomas. (PMID:28030816)
• Findings suggest that the miR-654-5p/GRAP/Ras/Erk signaling pathway in OSCC cells might contribute to the underlying mechanism through which miR-654-5p participates in the regulation of OSCC progression. (PMID:29364705)
• The CircHIPK3 could promote IGF2BP3 expression via interacting with miR-654 in glioma cells. (PMID:30057315)
• CDCP1 and PLAGL2 oncogenes were found to be the most relevant direct miR-654-5p targets and both genes convey in a molecular signature associated with key cancer pathways relevant to ovarian tumorigenesis, such as MYC, WNT and AKT pathways. This unveiled the tumor suppressor function of miR-654-5p, suggesting that its restoration or co-targeting of CDCP1 and PLAGL2 may be an effective therapeutic approach for cancer. (PMID:31278368)
• MiRNA Regulation of MIF in SLE and Attenuation of Murine Lupus Nephritis With miR-654. (PMID:31608058)
• Study found that miR-654 was up-regulated in gastric cancer (GC) tissues and cells, and promoted the proliferation of GC cells. miR-654 can inhibit the protein expression of p21 in GC cells, whereas circRHOBTB3 enhanced the protein expression of p21. circRHOBTB3 inhibited the activity of miR-654, and thus, reversed the miR- 654 induced cell proliferation and upregulated the protein expression of p21 indirectly. (PMID:31928527)
• miR-654-3p predicts the prognosis of hepatocellular carcinoma and inhibits the proliferation, migration, and invasion of cancer cells. (PMID:32176631)
• MiR-654-5p regulated cell progression and tumor growth through targeting SIRT6 in osteosarcoma. (PMID:32329825)
• Circular RNA hsa_circ_0085131 is involved in cisplatin-resistance of non-small-cell lung cancer cells by regulating autophagy. (PMID:32449799)
• Long noncoding RNA MKLN1AS aggravates hepatocellular carcinoma progression by functioning as a molecular sponge for miR6543p, thereby promoting hepatomaderived growth factor expression. (PMID:33000222)
• Long intergenic noncoding RNA LINC01232 contributes to esophageal squamous cell carcinoma progression by sequestering microRNA6543p and consequently promoting hepatomaderived growth factor expression. (PMID:33125097)
• Hsa_circ_0079480 promotes tumor progression in acute myeloid leukemia via miR-654-3p/HDGF axis. (PMID:33290265)
• miR6543p suppresses cell viability and promotes apoptosis by targeting RASAL2 in nonsmallcell lung cancer. (PMID:33300072)
• miR6545p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration. (PMID:33846806)
• LncRNA TP73-AS1 promotes oxidized low-density lipoprotein-induced apoptosis of endothelial cells in atherosclerosis by targeting the miR-654-3p/AKT3 axis. (PMID:34103010)
• Interference with circRNA DOCK1 inhibits hepatocellular carcinoma cell proliferation, invasion and migration by regulating the miR-654-5p/SMAD2 axis. (PMID:34184075)
• LINC02532 Contributes to Radiosensitivity in Clear Cell Renal Cell Carcinoma through the miR-654-5p/YY1 Axis. (PMID:34834139)
• CircRNA CORO1C Regulates miR-654-3p/USP7 Axis to Mediate Laryngeal Squamous Cell Carcinoma Progression. (PMID:35064359)
• EMX2OS Delays Wilms’Tumor Progression via Targeting miR-654-3p. (PMID:35181613)
• miR-654-5p Suppresses Migration and Proliferation of Vascular Smooth Muscle Cells by Targeting ADAMTS-7. (PMID:35462367)
• Comprehensive Analysis of hsa-miR-654-5p’s Tumor-Suppressing Functions. (PMID:35742854)
• LncRNA NEAT1 inhibits apoptosis and autophagy of ovarian granulosa cells through miR-654/STC2-mediated MAPK signaling pathway. (PMID:36634743)
• CircPLXNB2 regulates acute myeloid leukemia progression through miR-654-3p/CCND1 axis. (PMID:37272581)
• MiR-654-3p targets SRC to suppress tumor growth in non-small cell lung cancer. (PMID:37329531)
• Long noncoding RNA LINC00885 upregulates NCK1 to promote cell viability and migration of triple-negative breast cancer cells through sponging miR-654-3p. (PMID:37694355)
• Circ_0084188 promotes colorectal cancer progression by sponging miR-654-3p and regulating kruppel-like factor 12. (PMID:37698263)
• Circular RNA EFR3A promotes nasopharyngeal carcinoma progression through modulating the miR-654-3p/EFR3A axis. (PMID:38063110)

Cross-species orthologs

0 orthologs

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.