MIR655

Summary

MIR655 (microRNA 655, HGNC:32911) is a microRNA gene on chromosome 14q32.31.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 724025 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32911
Approved symbol	MIR655
Name	microRNA 655
Location	14q32.31
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-655
Ensembl gene	ENSG00000207646
Ensembl biotype	miRNA
Entrez	724025
RNAcentral	URS000075B3AB — miRNA, 97 nt, 1 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362159

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362159 — 1 exons

Exon	Start	End
ENSE00001436922	101049550	101049646

Expression profiles

Bgee: expression breadth broad, 53 present calls, max score 93.21.

Top tissues by expression

53 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adrenal tissue	UBERON:0018303	93.21	gold quality
lung	UBERON:0002048	84.29	gold quality
stomach	UBERON:0000945	80.05	gold quality
placenta	UBERON:0001987	79.69	gold quality
body of pancreas	UBERON:0001150	77.20	gold quality
heart	UBERON:0000948	73.27	gold quality
adenohypophysis	UBERON:0002196	73.26	gold quality
gastrocnemius	UBERON:0001388	72.89	gold quality
right atrium auricular region	UBERON:0006631	72.14	gold quality
kidney	UBERON:0002113	72.03	gold quality
intestine	UBERON:0000160	71.51	gold quality
left ovary	UBERON:0002119	71.47	gold quality
colon	UBERON:0001155	70.69	gold quality
right coronary artery	UBERON:0001625	69.51	gold quality
left adrenal gland cortex	UBERON:0035825	69.41	gold quality
lower esophagus muscularis layer	UBERON:0035833	68.92	gold quality
omental fat pad	UBERON:0010414	68.88	gold quality
left adrenal gland	UBERON:0001234	68.78	gold quality
esophagus mucosa	UBERON:0002469	68.27	gold quality
descending thoracic aorta	UBERON:0002345	68.08	gold quality
cerebellar hemisphere	UBERON:0002245	67.83	gold quality
Ammon’s horn	UBERON:0001954	67.73	gold quality
ascending aorta	UBERON:0001496	67.50	gold quality
muscle layer of sigmoid colon	UBERON:0035805	67.49	gold quality
subcutaneous adipose tissue	UBERON:0002190	67.33	gold quality
thoracic mammary gland	UBERON:0005200	67.17	gold quality
anterior cingulate cortex	UBERON:0009835	67.00	gold quality
right hemisphere of cerebellum	UBERON:0014890	66.95	gold quality
transverse colon	UBERON:0001157	66.62	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	66.32	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.14

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 21)

• ZEB1 and TGFBR2 identified as direct targets of miR-655 (PMID:23690952)
• Data show that the miR-134/487b/655 cluster regulates TGF-beta1-induced epithelial-mesenchymal transition and affected the resistance to gefitinib by directly targeting membrane-associated guanylate kinase, WW, and PDZ domain-containing protein 2 (MAGI2). (PMID:24258346)
• Report feedback loop between PTTG1 targeting miRNAs,including mir655, PTTG1 and p53 that promotes pituitary tumorigenesis. (PMID:26320179)
• Results showed that MiR-655-3p was significantly decreased in hepatocellular carcinoma (HCC) clinical specimens and cell lines. Overexpression of miR-655-3p suppressed cell proliferation, migration and invasion of HCC in vitro. The study suggests that MiR-655-3p might functions as a tumor suppressor by directly targeting ADAM10 and indirectly regulating beta-catenin pathway in the development of progression of HCC. (PMID:27259866)
• MiR-655-3p showed a 6.79-fold decrease in expression after 12 h exposure compared to 0 h, was predicted in silico to bind ABCG2 3’-UTR and showed a significant negative correlation (p = 0.01) to ABCG2 expression level. (PMID:28990842)
• miR655 is an oncogenic miRNA in human breast cancer, promoting EMT, cellular migration, invasion, proliferation, SLC stimulation and metastasis in vivo. (PMID:29321644)
• Low levels of miR-655 in plasma relate to lymphatic progression. (PMID:30609933)
• MiR-655-3p inhibited proliferation and migration of ovarian cancer cells by targeting RAB1A. (PMID:31114987)
• MicroRNA-655-3p and microRNA-497-5p inhibit cell proliferation in cultured human lip cells through the regulation of genes related to human cleft lip (PMID:31122291)
• This study establishes the dynamic functions of miR526b/miR655 in oxidative stress induction in breast cancer. (PMID:31430859)
• SP1-induced lncRNA LINC00689 overexpression contributes to osteosarcoma progression via the miR-655/SOX18 axis. (PMID:32196572)
• MiR-655-3p inhibits the progression of osteoporosis by targeting LSD1 and activating BMP-2/Smad signaling pathway. (PMID:32431171)
• TUG1 weakens the sensitivity of acute myeloid leukemia cells to cytarabine by regulating miR-655-3p/CCND1 axis. (PMID:32432757)
• Long noncoding RNA LINC01194 enhances the malignancy of laryngeal squamous cell carcinoma by sponging miR-655 to increase SOX18 expression. (PMID:32703403)
• MiR-655-3p inhibits growth and invasiveness of trophoblasts via targeting PBX3 and thus deteriorates preeclampsia. (PMID:33155190)
• Alteration of Pituitary Tumor Transforming Gene 1 by MicroRNA-186 and 655 Regulates Invasion Ability of Human Oral Squamous Cell Carcinoma. (PMID:33498448)
• Circular RNA circ_0001955 promotes hepatocellular carcinoma tumorigenesis by up-regulating alkaline ceramidase 3 expression through microRNA-655-3p. (PMID:35034572)
• Long noncoding RNA endogenous bornavirus-like nucleoprotein acts as an oncogene by regulating microRNA-655-3p expression in T-cell acute lymphoblastic leukemia. (PMID:35220878)
• CCN2 Facilitates IL-17 Production and Osteoclastogenesis in Human Osteoarthritis Synovial Fibroblasts by Inhibiting miR-655 Expression. (PMID:35876037)
• Exosomal miR-655-3p inhibits growth, and invasion and macrophage M2 polarization through targeting CXCR4 in papillary thyroid carcinoma. (PMID:36512648)
• Circ-LDLRAD3/miR-655-3p/MAPK1 axis enhances cell migration and invasion in papillary thyroid carcinoma. (PMID:38430029)

Cross-species orthologs

0 orthologs

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.