MIR656

Summary

MIR656 (microRNA 656, HGNC:32912) is a microRNA gene on chromosome 14q32.31.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 724026 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32912
Approved symbol	MIR656
Name	microRNA 656
Location	14q32.31
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-656
Ensembl gene	ENSG00000207959
Ensembl biotype	miRNA
OMIM	616376
Entrez	724026
RNAcentral	URS000075B5E4 — miRNA, 78 nt, 15 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385224

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385224 — 1 exons

Exon	Start	End
ENSE00001500230	101066724	101066801

Expression profiles

Bgee: expression breadth ubiquitous, 101 present calls, max score 95.11.

Top tissues by expression

101 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adrenal tissue	UBERON:0018303	95.11	gold quality
placenta	UBERON:0001987	89.83	gold quality
left adrenal gland cortex	UBERON:0035825	87.14	gold quality
adrenal gland	UBERON:0002369	86.00	gold quality
skeletal muscle tissue	UBERON:0001134	85.80	gold quality
left adrenal gland	UBERON:0001234	85.07	gold quality
right adrenal gland	UBERON:0001233	84.74	gold quality
right adrenal gland cortex	UBERON:0035827	84.60	gold quality
right hemisphere of cerebellum	UBERON:0014890	80.89	gold quality
pituitary gland	UBERON:0000007	80.87	gold quality
left ovary	UBERON:0002119	80.61	gold quality
body of pancreas	UBERON:0001150	80.53	gold quality
pancreas	UBERON:0001264	80.18	gold quality
adenohypophysis	UBERON:0002196	79.64	gold quality
ovary	UBERON:0000992	79.59	gold quality
blood	UBERON:0000178	78.38	gold quality
cerebellar hemisphere	UBERON:0002245	78.35	gold quality
endometrium	UBERON:0001295	77.69	gold quality
right ovary	UBERON:0002118	76.47	gold quality
muscle of leg	UBERON:0001383	75.87	gold quality
left uterine tube	UBERON:0001303	75.14	gold quality
fundus of stomach	UBERON:0001160	74.75	gold quality
islet of Langerhans	UBERON:0000006	74.59	gold quality
stomach	UBERON:0000945	74.23	gold quality
fallopian tube	UBERON:0003889	74.07	gold quality
gastrocnemius	UBERON:0001388	73.86	gold quality
smooth muscle tissue	UBERON:0001135	73.72	gold quality
rectum	UBERON:0001052	73.56	gold quality
body of stomach	UBERON:0001161	73.00	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	72.67	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.16

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 11)

• Low MIRN656 expression is associated with glioma tumorigenesis. (PMID:24480809)
• Mir656 underexpression is associated with glioblastoma. (PMID:29769662)
• Study performed the expression analysis of miR-379/miR-656 miRNA-cluster (C14MC) in oligodendrogliomas (ODGs) and also investigated the mechanism underlying modulation of this cluster; and found significant downregulation of majority of the miRNAs. Further data from The Cancer Genome Atlas (TCGA) also confirmed the global downregulation of C14MC. (PMID:29931616)
• LncRNA SNHG14 aggravates invasion and migration as ceRNA via regulating miR-656-3p/SIRT5 pathway in hepatocellular carcinoma. (PMID:32607966)
• CircRNA circBACH1 (hsa_circ_0061395) serves as a miR-656-3p sponge to facilitate hepatocellular carcinoma progression through increasing SERBP1 expression. (PMID:33831787)
• MicroRNA-656-3p inhibits colorectal cancer cell migration, invasion, and chemo-resistance by targeting sphingosine-1-phosphate phosphatase 1. (PMID:35081855)
• YY1-induced long non-coding RNA small nucleolar RNA host gene 8 promotes the tumorigenesis of melanoma via the microRNA-656-3p/SERPINE1 mRNA binding protein 1 axis. (PMID:35156513)
• Circ_0028007 Aggravates the Malignancy of Nasopharyngeal Carcinoma by Regulating miR-656-3p/ELF2 Axis. (PMID:35239093)
• Circular RNA hsa_circ_0018189 drives non-small cell lung cancer growth by sequestering miR-656-3p and enhancing xCT expression. (PMID:36164726)
• Silencing of long non-coding RNA SDCBP2-AS1/microRNA-656-3p/CRIM1 axis promotes ferroptosis of lung cancer cells. (PMID:37807311)
• Regulation and tumor-suppressive function of the miR-379/miR-656 (C14MC) cluster in cervical cancer. (PMID:38400534)

Cross-species orthologs

0 orthologs

Paralogs (16): MIR494 (ENSG00000194717), MIR377 (ENSG00000199015), MIR381 (ENSG00000199020), MIR369 (ENSG00000199025), MIR323A (ENSG00000199069), MIR410 (ENSG00000199092), MIR409 (ENSG00000199107), MIR539 (ENSG00000202560), MIR487A (ENSG00000207742), MIR487B (ENSG00000207754), MIR496 (ENSG00000207961), MIR154 (ENSG00000207978), MIR323B (ENSG00000208004), MIR1185-1 (ENSG00000221525), MIR1185-2 (ENSG00000221614), MIR382 (ENSG00000283170)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.