MIR663A

Summary

MIR663A (microRNA 663a, HGNC:32919) is a microRNA gene on chromosome 20p11.1.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 724033 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32919
Approved symbol	MIR663A
Name	microRNA 663a
Location	20p11.1
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-663, miR-663a
Ensembl gene	ENSG00000284419
Ensembl biotype	miRNA
Entrez	724033
RNAcentral	URS00006A470C — miRNA, 93 nt, 1 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385250

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385250 — 1 exons

Exon	Start	End
ENSE00001500256	26208186	26208278

Expression profiles

Bgee: expression breadth ubiquitous, 111 present calls, max score 99.82.

Top tissues by expression

111 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
sural nerve	UBERON:0015488	99.82	gold quality
mucosa of transverse colon	UBERON:0004991	99.41	gold quality
lower esophagus mucosa	UBERON:0035834	98.27	gold quality
right uterine tube	UBERON:0001302	96.87	gold quality
right testis	UBERON:0004534	96.73	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	96.45	gold quality
metanephros cortex	UBERON:0010533	95.87	gold quality
ectocervix	UBERON:0012249	95.57	gold quality
right lobe of liver	UBERON:0001114	95.55	gold quality
liver	UBERON:0002107	95.45	gold quality
minor salivary gland	UBERON:0001830	95.34	gold quality
tibial nerve	UBERON:0001323	95.27	gold quality
left testis	UBERON:0004533	95.22	gold quality
testis	UBERON:0000473	94.90	gold quality
transverse colon	UBERON:0001157	94.84	gold quality
subcutaneous adipose tissue	UBERON:0002190	94.84	gold quality
adult mammalian kidney	UBERON:0000082	94.77	gold quality
adrenal tissue	UBERON:0018303	94.73	gold quality
saliva-secreting gland	UBERON:0001044	94.72	gold quality
prostate gland	UBERON:0002367	94.72	gold quality
thoracic mammary gland	UBERON:0005200	94.70	gold quality
skin of abdomen	UBERON:0001416	94.53	gold quality
fundus of stomach	UBERON:0001160	94.24	gold quality
granulocyte	CL:0000094	94.20	gold quality
small intestine Peyer’s patch	UBERON:0003454	94.13	gold quality
zone of skin	UBERON:0000014	94.06	gold quality
small intestine	UBERON:0002108	93.99	gold quality
right ovary	UBERON:0002118	93.88	gold quality
skin of leg	UBERON:0001511	93.81	gold quality
intestine	UBERON:0000160	93.70	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.23

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• mir-663 might function as a potent suppressor of tumor growth. (PMID:20514450)
• MicroRNA-663 upregulated by oscillatory shear stress plays a role in inflammatory response of endothelial cells. (PMID:21378144)
• Results suggest that lentivirus delivery of miR-663 could potentially be used directly as an anticancer treatment in hematological malignancies. (PMID:21518431)
• Hsa-miR-663 can bind to the REN and APOE 3’ untranslated regions and can regulate REN and APOE mRNA levels. (PMID:22042811)
• miR-663 contributed to lung cancer cell proliferation of by regulating TGFB1, P53, Bax, and Fas directly or indirectly. (PMID:22393947)
• miR-663 is critically important for 2 key events induced in ECs by stress agents and oxidized lipids, namely induction of transcription factor ATF4 and its downstream gene VEGF (PMID:22776647)
• The expression of these miRNAs-663 and -638 might be useful as novel diagnostic and prognostic markers in patients with tumors. (PMID:23155245)
• Expression of miR-663 was significantly lower in pediatric acute myeloid leukemia (AML) cells compared to NBM controls; a high frequency of miR-663 promoter hypermethylation was observed in both AML cell lines and pediatric AML samples (PMID:23870168)
• Over-expression of miR-663 may suppress proliferation of the k-562 cell line in part by enhancing cell apoptosis (PMID:23953123)
• MicroRNA-663 regulates human vascular smooth muscle cell phenotypic switch and vascular neointimal formation. (PMID:24014830)
• these findings suggest that miR-663 is a potential oncomiR for Castration-resistant prostate cancer and may serve as a tumor biomarker for the early diagnosis of CRPC. (PMID:24243035)
• miR-663 is a novel prognostic biomarker and a potential therapeutic candidate for glioblastoma. (PMID:24523440)
• We present evidence for an association of miR-663 upon stress and chronological age. (PMID:24664125)
• the present study demonstrated that waltonitone induced apoptosis of lung cancer cells through, at least partly, miR-663-induced Bcl-2 downregulation. (PMID:25301444)
• results indicate that AICD has a novel role in suppression of neuronal differentiation via transcriptional regulation of miR-663 in human neural stem cells. (PMID:25695604)
• HER2 inhibition can enhance or restore ER expression with parallel Bcl2 upregulation, representing an ER-dependent survival mechanism potentially leading to anti-HER2 resistance. (PMID:26023083)
• increased miR-663 suppressed adenomatous polyposis coli (APC) protein expression significantly, and this down-regulation of APC expression triggered activation of canonical Wnt signaling through accumulation of beta-catenin in fibroblast-like synoviocytes (PMID:26028359)
• miR-663 expression was inhibited in papillary thyroid carcinoma tissue samples and cell lines. There were statistically significant differences in expression of miR-663 with regard to age and tumor size. Upregulation of miR-663 suppressed PTC cell invasion and migration. By targeting TGFbeta1, miR-663 efficiently regulates the expression of epithelial-mesenchymal transition markers and matrix metalloproteinases. (PMID:26687649)
• The analyses using human breast ductal carcinoma tissues exhibited that the expression of ZNF224 and miR-663a was increased in cancer compared to non-cancer region. (PMID:27105517)
• MiR-663a suppresses proliferation and invasion by targeting AP-1 component JunD in non-small cell lung cancer cells. (PMID:27184257)
• Proteomic analysis revealed that PLOD3, which is the gene encoding for collagen-modifying lysyl hydroxylase 3 (LH3), is regulated by miR-663a. (PMID:27233793)
• miR-663a inhibits hepatocellular carcinoma cell proliferation and motility by targeting HMGA2. (PMID:27261623)
• The upregulation of miR-572 and miR-663a was consistent in both the SH-SY5Y and SK-N-SH cells. (PMID:27716787)
• This is the first report showing that aberrant DNA methylation of the miR-663a promoter can occur in normal tissue of the cancer patients, suggesting a possible link between this epigenetic abnormality and endometrial cancer. (PMID:28440489)
• MiR-663 can significantly increase the proliferation of PCa cells. (PMID:28952221)
• miR663 may act as a tumor suppressor in CRC by directly targeting FSCN1 (PMID:29039557)
• Data suggest that oxidative stress and reactive oxygen species play key roles in epigenetic regulation of microRNA-663; microRNA-663 appears to regulate expression of nuclear-encoded respiratory chain subunits in tumor cells; increased microRNA-663 expression in breast tumors correlates with increased patient survival. (PMID:29066618)
• Results show that miR-663 depletion is sufficient to elicit cell death in NSCLC cells and to impair tumor growth in vivo. (PMID:29352138)
• The expression of miR-663a was up-regulated in renal cell carcinoma cells and tissues. (PMID:30021352)
• A negative correlation between miR-663a and TGF-beta1 expression was also confirmed from the clinical samples of HCC. (PMID:30486878)
• miR-663 may function as an ‘apoptomiR’ by inhibiting the anti-apoptotic gene AATF to induce apoptosis. These findings could have therapeutic implications for epithelial cell targeting in cancer therapy. (PMID:30610504)
• Study of tissue samples from 172 patients with colon cancer (CC) identified that miR663a was significantly downregulated in CC, particularly in metastatic CC (P=0.044). miR663a overexpression inhibited the proliferation and migration/invasion of CC cells in vitro, and tumor growth and metastasis of CC cells in vivo. (PMID:30664167)
• this study shows that microRNA-663 induces immune dysregulation by inhibiting TGF-beta1 production in bone marrow-derived mesenchymal stem cells in patients with systemic lupus erythematosus (PMID:30886422)
• Plasma miR-663a was identified as a novel potential biomarker for the diagnosis of osteosarcoma (PMID:30987831)
• SNHG8 was up-regulated in colorectal cancer and promoted the proliferation, migration, and invasion of colorectal cancer by sponging miR-663. (PMID:31152930)
• miR-663 was up-regulated in CRC patients and may be an effective biomarker for CRC diagnosis (PMID:31240955)
• MiR-663a, regulated by lncRNA GAS5, contributes to osteosarcoma development through targeting MYL9. (PMID:32633150)
• LncRNA PLAC2 upregulates miR-663 to downregulate TGF-beta1 and suppress bladder cancer cell migration and invasion. (PMID:32650766)
• Functional classification of prostate cancerassociated miRNAs through CRISPR/Cas9mediated gene knockout. (PMID:32901864)
• MicroRNA-663 prevents monocrotaline-induced pulmonary arterial hypertension by targeting TGF-beta1/smad2/3 signaling. (PMID:34339758)

Cross-species orthologs

0 orthologs

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.