MIR664B

Summary

MIR664B (microRNA 664b, HGNC:43501) is a microRNA gene on chromosome Xq28.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 100847052 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:43501
Approved symbol	MIR664B
Name	microRNA 664b
Location	Xq28
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-664b
Ensembl gene	ENSG00000284450
Ensembl biotype	miRNA
Entrez	100847052
RNAcentral	URS000075DD29 — ncRNA, 61 nt, 4 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000626756

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000626756 — 1 exons

Exon	Start	End
ENSE00003766657	154768596	154768656

Expression profiles

Bgee: expression breadth broad, 11 present calls, max score 68.95.

Top tissues by expression

11 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
zone of skin	UBERON:0000014	68.95	gold quality
caudate nucleus	UBERON:0001873	68.83	gold quality
lower esophagus muscularis layer	UBERON:0035833	68.56	gold quality
muscle layer of sigmoid colon	UBERON:0035805	67.11	gold quality
skin of abdomen	UBERON:0001416	66.04	gold quality
esophagus mucosa	UBERON:0002469	64.47	gold quality
amygdala	UBERON:0001876	64.33	gold quality
subcutaneous adipose tissue	UBERON:0002190	63.57	gold quality
tibial nerve	UBERON:0001323	61.49	gold quality
omental fat pad	UBERON:0010414	60.24	gold quality
upper lobe of left lung	UBERON:0008952	55.78	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 7)

• miR-664 functions as an oncogene miRNA and has an important role in promoting human osteosarcoma cell proliferation by suppressing FOXO4 expression. (PMID:26463624)
• miR-664 was remarkably high in cutaneous squamous cell carcinomas (cSCC) patient specimens and cSCC cell lines. Study identified IRF2 as a direct downstream target of miR-664. Knockdown of IRF2 reverses pro-tumorigenesis phenotype of miR-664; whereas IRF2 over-expression inhibits miR-664 tumorigenesis in cSCC. Together, it revealed miR-664 functions as an oncogene in cSCC via suppression of IRF2. (PMID:31138473)
• miR-664b-5p Inhibits Hepatocellular Cancer Cell Proliferation Through Targeting Oncogene AKT2. (PMID:31967930)
• lncRNA RASSF8AS1 suppresses the progression of laryngeal squamous cell carcinoma via targeting the miR664b3p/TLE1 axis. (PMID:33000257)
• Downregulation of ARMC8 promotes tumorigenesis through activating Wnt/beta-catenin pathway and EMT in cutaneous squamous cell carcinomas. (PMID:34016486)
• miR-664b-3p inhibits colon cell carcinoma via negatively regulating Budding uninhibited by benzimidazole 3. (PMID:35156516)
• Long noncoding RNA HOXD-AS1 promotes the progression of pancreatic cancer through miR-664b-3p/PLAC8 axis. (PMID:35279480)

Cross-species orthologs

0 orthologs

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.