MIR665

Summary

MIR665 (microRNA 665, HGNC:33662) is a microRNA gene on chromosome 14q32.2.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 100126315 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:33662
Approved symbol	MIR665
Name	microRNA 665
Location	14q32.2
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-665
Ensembl gene	ENSG00000283159
Ensembl biotype	miRNA
Entrez	100126315
RNAcentral	URS000075CD82 — miRNA, 72 nt, 11 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000635949

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000635949 — 1 exons

Exon	Start	End
ENSE00003800484	100875033	100875104

Expression profiles

Bgee: expression breadth broad, 67 present calls, max score 87.88.

Top tissues by expression

67 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adrenal tissue	UBERON:0018303	87.88	gold quality
lung	UBERON:0002048	84.28	gold quality
muscle of leg	UBERON:0001383	75.58	gold quality
gastrocnemius	UBERON:0001388	74.58	gold quality
placenta	UBERON:0001987	73.50	gold quality
stomach	UBERON:0000945	72.65	gold quality
right adrenal gland cortex	UBERON:0035827	71.84	gold quality
heart	UBERON:0000948	71.44	gold quality
heart left ventricle	UBERON:0002084	71.22	gold quality
left adrenal gland cortex	UBERON:0035825	71.17	gold quality
monocyte	CL:0000576	71.06	gold quality
islet of Langerhans	UBERON:0000006	70.87	gold quality
right adrenal gland	UBERON:0001233	70.13	gold quality
adenohypophysis	UBERON:0002196	69.45	gold quality
liver	UBERON:0002107	69.44	gold quality
left adrenal gland	UBERON:0001234	69.36	gold quality
Brodmann (1909) area 9	UBERON:0013540	69.35	gold quality
body of stomach	UBERON:0001161	68.98	gold quality
intestine	UBERON:0000160	68.82	gold quality
right atrium auricular region	UBERON:0006631	68.29	gold quality
body of pancreas	UBERON:0001150	68.28	gold quality
transverse colon	UBERON:0001157	68.27	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	68.26	gold quality
Ammon’s horn	UBERON:0001954	68.13	gold quality
left ovary	UBERON:0002119	67.63	gold quality
endometrium	UBERON:0001295	67.60	gold quality
substantia nigra	UBERON:0002038	67.27	gold quality
ascending aorta	UBERON:0001496	67.07	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	66.55	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	66.42	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.48

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 39)

• findings show CB1 receptors are regulated by miR-494 and CB2 receptors are targeted by miR-665;in chronic heart failure (CHF) miR-665 expression is significantly decreased and miR-494 is slightly increased; results suggest that in CHF, altered expression of specific miRNAs may contribute to a compensatory response of diseased myocardium (PMID:25111814)
• hsa-miR-665 and hsa-miR-95 were downregulated in GSRCC but upregulated in intestinal gastric adenocarcinoma, and the relatively differential expression of the miRNAs negatively controlling their target genes, GLI2 and PLCG1 (PMID:25964059)
• miR-665 functions in an miRNA-epigenetic regulatory network to control dentinogenesis. (PMID:26124283)
• Data show that four cardiac fibroblast-derived microRNAS miR-660-3p, miR-665, miR-1285-3p and miR-4491 were found significantly upregulated in heart and plasma during heart failure. (PMID:26683101)
• Mir665 overexpression is associated with metastasis in osteosarcoma. (PMID:26988989)
• MiR-665 induced apoptosis by inhibiting XBP1 and ORMDL3. (PMID:28333149)
• miR-665-3p regulates autophagy by targeting ATG4B expression to relieve inflammation and apoptosis in intestinal ischemia/reperfusion. (PMID:29706629)
• These results suggested that dysregulated miR-665 expression might act an important role in the development of intervertebral disc degeneration. (PMID:29761869)
• MiR665 downregulated the expression of HOXA10 and weakened the ability of ovarian cancer cells to proliferate and migrate. miR665, through targeting HOXA10, serves as a suppressor gene in ovarian cancer. (PMID:30015865)
• demonstrated that miR-665 played an important role in heart failure via damaging coronary microvessel angiogenesis (PMID:30243022)
• lnc-DANCR-mediated miR-665 downregulation regulates the malignant phenotype of CC cells. (PMID:30582654)
• Ectopic miR-665 expression promotes epithelial-mesenchymal transition (EMT), proliferation, migration and invasion of BC cells, and increases tumor growth and metastasis of BC in mice (PMID:31209222)
• EXT1- or miR-665-targeted strategies can confer the therapeutic effect of promoting apoptosis by deactivating the ERK1/2 pathway. (PMID:31465316)
• miR-665 promotes the progression of gastric adenocarcinoma via elevating FAK activation through targeting SOCS3 and is negatively regulated by lncRNA MEG3. (PMID:31650535)
• LncRNA SND1-IT1 accelerates the proliferation and migration of osteosarcoma via sponging miRNA-665 to upregulate POU2F1. (PMID:31799644)
• microRNA-665 is down-regulated in gastric cancer and inhibits proliferation, invasion, and EMT by targeting PPP2R2A. (PMID:31923339)
• [MiR-665 Promotes the Biological Behavior of Small Cell Lung Cancer by Targeting LLGL1]. (PMID:32222154)
• Long noncoding RNA LINC00205 enhances the malignant characteristics of retinoblastoma by acting as a molecular sponge of microRNA-665 and consequently increasing HMGB1 expression. (PMID:32223925)
• MicroRNA-665-3p attenuates oxygen-glucose deprivation-evoked microglial cell apoptosis and inflammatory response by inhibiting NF-kappaB signaling via targeting TRIM8. (PMID:32512270)
• Circular RNA Paired-Related Homeobox 1 Promotes Gastric Carcinoma Cell Progression via Regulating MicroRNA-665/YWHAZ Axis. (PMID:33201331)
• MiR-665 inhibits inflammatory response in microglia following spinal cord injury by targeting TREM2. (PMID:33506893)
• MicroRNA-665 Regulates Cell Proliferation and Apoptosis of Vascular Smooth Muscle Cells by Targeting TGFBR1. (PMID:33731513)
• miR-665 inhibits epithelial-to-mesenchymal transition in bladder cancer via the SMAD3/SNAIL axis. (PMID:34196584)
• LncRNA MIAT regulates autophagy and apoptosis of macrophage infected by Mycobacterium tuberculosis through the miR-665/ULK1 signaling axis. (PMID:34454184)
• RNF144A-AS1 promotes the development of glioma cells by targeting miR-665/HMGA1 axis. (PMID:34560189)
• Circ_SPG11 plays contributing effects on IL-1beta-induced chondrocyte apoptosis and ECM degradation via miR-665 inhibition-mediated GREM1 upregulation. (PMID:34798237)
• Circ_0044556 Promotes the Progression of Colorectal Cancer via the miR-665-Dependent Expression Regulation of Diaphanous Homolog 1. (PMID:34822025)
• MiR-665 suppresses the progression of diffuse large B cell lymphoma (DLBCL) through targeting LIM and SH3 protein 1 (LASP1). (PMID:34875555)
• Long non-coding RNA NHEG1/hsa-miR-665/HMGB1 axis is involved in the regulation of neuroblastoma progression. (PMID:34889712)
• MicroRNA-665-3p exacerbates nonalcoholic fatty liver disease in mice. (PMID:35038955)
• Circ_0101802 Facilitates Colorectal Cancer Progression Depending on the Regulation of miR-665/DVL3 Signaling. (PMID:35314912)
• LncRNA RPSAP52 promotes cell proliferation and inhibits cell apoptosis via modulating miR-665/STAT3 in gastric cancer. (PMID:35322746)
• Hsa_circ_0010729 is Involved in Oxygen-Glucose Deprivation/Reoxygenation-Induced Human Microvascular Endothelial Cell Deprivation by Targeting miR-665/ING5. (PMID:35482130)
• Circ_ARHGAP32 acts as miR-665 sponge to upregulate FGF2 to promote ox-LDL induced vascular smooth muscle cells proliferation and migration. (PMID:35662113)
• Sevoflurane Inhibits Metastasis in Hepatocellular Carcinoma by Inhibiting MiR-665-Induced Activation of the ERK/MMP Pathway. (PMID:35699095)
• Hsa_hsa_circ_0081069 promotes the progression of colorectal cancer through sponging miR-665 and regulating E2F3 expression. (PMID:36181281)
• Circ_0104700 contributes to acute myeloid leukemia progression by enhancing MCM2 expression through targeting miR-665. (PMID:37358551)
• circSNTB2 and CUL4A Induces Dysfunction of Nucleus Pulposus Cells by Competitively Binding miR-665. (PMID:37507642)
• Circular RNA circESYT2 serves as a microRNA-665 sponge to promote the progression of hepatocellular carcinoma through ENO2. (PMID:38710213)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir665	ENSMUSG00000076313
rattus_norvegicus	Mir665	ENSRNOG00000040355

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.