MIR708

Summary

MIR708 (microRNA 708, HGNC:33654) is a microRNA gene on chromosome 11q14.1.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 100126333 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:33654
Approved symbol	MIR708
Name	microRNA 708
Location	11q14.1
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-708
Ensembl gene	ENSG00000211997
Ensembl biotype	miRNA
OMIM	620733
Entrez	100126333
RNAcentral	URS000062A2F0 — miRNA, 88 nt, 22 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000390708

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000390708 — 1 exons

Exon	Start	End
ENSE00001508385	79402022	79402109

Expression profiles

Bgee: expression breadth broad, 86 present calls, max score 81.00.

Top tissues by expression

86 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adrenal tissue	UBERON:0018303	81.00	gold quality
right coronary artery	UBERON:0001625	80.73	gold quality
monocyte	CL:0000576	78.76	gold quality
blood	UBERON:0000178	74.87	gold quality
islet of Langerhans	UBERON:0000006	73.93	gold quality
adult mammalian kidney	UBERON:0000082	73.62	gold quality
gastrocnemius	UBERON:0001388	72.58	gold quality
left adrenal gland cortex	UBERON:0035825	72.46	gold quality
prefrontal cortex	UBERON:0000451	72.31	gold quality
heart	UBERON:0000948	71.97	gold quality
skin of abdomen	UBERON:0001416	71.52	gold quality
left ovary	UBERON:0002119	71.21	gold quality
zone of skin	UBERON:0000014	71.20	gold quality
ovary	UBERON:0000992	71.20	gold quality
stomach	UBERON:0000945	71.19	gold quality
left adrenal gland	UBERON:0001234	70.90	gold quality
skin of leg	UBERON:0001511	70.85	gold quality
anterior cingulate cortex	UBERON:0009835	70.19	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	70.16	gold quality
body of stomach	UBERON:0001161	70.06	gold quality
right ovary	UBERON:0002118	69.77	gold quality
heart left ventricle	UBERON:0002084	69.70	gold quality
tibial artery	UBERON:0007610	69.51	gold quality
lower esophagus muscularis layer	UBERON:0035833	69.26	gold quality
omental fat pad	UBERON:0010414	69.12	gold quality
thoracic aorta	UBERON:0001515	68.92	gold quality
ascending aorta	UBERON:0001496	68.90	gold quality
adipose tissue	UBERON:0001013	68.72	gold quality
thoracic mammary gland	UBERON:0005200	68.67	gold quality
granulocyte	CL:0000094	68.58	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.99

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): FLI1

Literature-anchored findings (GeneRIF, showing 40)

• a major tumor suppressive role for miR-708, which may offer an attractive new target for prognostic and therapeutic intervention in renal cell carcinoma (PMID:21852381)
• our findings suggest that reduced miR-708 expression leads to prostate cancer initiation, progression, and development by regulating the expression of CD44 as well as AKT2. (PMID:22552290)
• miRNA-708 acts as an oncogene contributing to tumor growth and disease progression by directly downregulating TMEM88 (PMID:22573352)
• we show that high levels of EYA3 significantly correlate with low levels of miR-708 in Ewing sarcoma samples, suggesting that this miR-mediated mechanism of EYA3 regulation holds true in human cancers (PMID:22723308)
• MIR-708 targets the endoplasmic reticulum protein neuronatin to decrease intracellular calcium level, resulting in reduction of activation of ERK and FAK, decreased cell migration, and impaired metastases. (PMID:23328481)
• MiR-708 may act as an oncogene and induce the carcinogenicity of bladder cancer by down-regulating Caspase-2 level. (PMID:23568547)
• MiR-708 may play an important role as a tumor suppressor in GBM. (PMID:23754151)
• In airway smooth muscle cells, miR-708 regulates CD38 expression. (PMID:25175907)
• Glucocorticoids mediate induction of microRNA-708 to suppress ovarian cancer metastasis through targeting Rap1B. (PMID:25569036)
• MiR-708 regulates the NF-kappaB pathway by targeting IKKbeta. (PMID:25704289)
• Expression levels of miR-708 in hepatocellular carcinoma tissues was significantly lower compared to non-cancerous tissues. (PMID:26211597)
• Low expression of miR-708-5p was associated with metastatic lung cancer. (PMID:26678031)
• our findings showed that miR-708 might serve as an antioncogene by directly targeting LSD1, through promoting cell growth and cell invasion. (PMID:26833707)
• High miR-708 expression is associated with non-small cell lung cancer. (PMID:26870998)
• determined that GC use resulted in overexpression of miR-708 in MSCs, and thus, targeting miR-708 may serve as a novel therapeutic biomarker for the prevention and treatment of ONFH (PMID:26932538)
• These results demonstrate that miR-708 and miR-140-3p exert distinct effects on inflammation-associated gene expression and biological function of airway smooth muscle cells. (PMID:26998837)
• miRNA-708 acts as a tumor suppressor because it negatively regulates the anti-apoptotic protein c-FLIPL and regulates the sensitivity of renal cancer cells to various apoptotic stimuli. (PMID:27092874)
• Single recurrent variant located near the miR-708 gene may have a role in BD and schizophrenia susceptibility. (PMID:27864917)
• Obtained results indicate that miRNAs: miR-499, miR-708 and miR-1908 are the most promising candidates for biomarkers of depression episodes of bipolar disorder. (PMID:28647289)
• miR-708 downregulated the expression of insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) and suppressed Akt phosphorylation. Silencing of IGF2BP1 markedly blocked the phosphorylation of Akt. (PMID:28685895)
• High miR708 expression is associated with Metastatic Renal Cell Carcinoma. (PMID:28963640)
• MiR-708-5p suppresses NSCLC initiation, development, and stemness through interfering DNMT3A-dependent DNA methylation. (PMID:28972040)
• miR-708 inhibited neuronal cell death in an in vitro model of ischemia where miR-708 decreased the rate of apoptosis of glucose-deprived reoxygenation (OGD/R) human neuroblastoma cells (SH-SY5Y)by targeting JAK1. (PMID:29128627)
• Data show that miR-708-3p expression level is downregulated in breast cancers and, mediates metastasis and chemoresistance through inhibition of epithelial-to-mesenchymal transition in breast cancer. These results suggest that miR-708-3p acts as a cancer suppressor miRNA in breast neoplasm. (PMID:29575368)
• The results strongly suggest that miR-708 and miR-34c, overexpressed in dystrophic context, are new actors involved in the regulation of nNOS expression in dystrophic muscle. (PMID:29703249)
• CA inhibits the proliferation and invasion of CML KBM-7 cells which could mainly be due to downregulation of microRNA- 780 expression. (PMID:30003745)
• MiRNA-708 expression was reduced in osteosarcoma tumor cell lines. (PMID:30229816)
• identified target genes and important signaling pathways involved in bone metabolism pointing hsa-miR-708-5p as a candidate marker for osteoporosis in Mexican population (PMID:30322266)
• Despite being downregulated in Ewing sarcoma tissue samples, miR-708-5p might represent a secondary genetic alteration derived from the pleiotropic cellular effects of the abnormal EWS/FLI1 transcription factor that does not affect tumor growth but instead, is related with the promotion of tumor invasion. (PMID:30470587)
• In vivo, miR708-NP directly targeted the SOX2/OCT4-mCherry+ miR-708(low) tumor cells to impair metastasis. Together, our preclinical findings provide a mechanism-based antimetastatic therapeutic approach for Triple-negative breast cancer (TNBC), with a marked potential to generate miR-708 replacement therapy for high-risk TNBC patients in the clinic. (PMID:30679387)
• We found that miRNA-708-5p was upregulated in pancreatic ductal adenocarcinoma tissues and cell lines, and high miRNA-708 expression indicated poor prognosis in pancreatic ductal adenocarcinoma patients. (PMID:30683474)
• GR agonists induced miR-708 and downstream suppression of NF-kappaB signaling. (PMID:30726934)
• miR-708 inhibited AKT/beta-catenin signaling, which is activated by SPHK2. In addition, miR-708 was transcriptionally repressed by EZH2 (enhancer of zeste homolog 2)-induced histone H3 lysine 27 trimethylation and promoter methylation. In summary, these findings revealed that miR-708 is a glioma tumor suppressor. (PMID:31171769)
• Metformin mediates induction of miR-708 to inhibit self-renewal and chemoresistance of breast cancer stem cells through targeting CD47. (PMID:31273952)
• that LOXL1-AS1/miR-708-5p/USF1 pathway contributed to the development of gastric cancer (PMID:31468594)
• MicroRNA-708-5p regulates mycobacterial vitality and the secretion of inflammatory factors in Mycobacterium tuberculosis-infected macrophages by targeting TLR4. (PMID:31599428)
• MicroRNA-708 is a novel regulator of the Hoxa9 program in myeloid cells. (PMID:31768018)
• The microRNA7085p/ZEB1/EMT axis mediates the metastatic potential of osteosarcoma. (PMID:31894343)
• MicroRNA-708 represses hepatic stellate cells activation and proliferation by targeting ZEB1 through Wnt/beta-catenin pathway. (PMID:31962101)
• MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. (PMID:32463570)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir708	ENSMUSG00000076143
rattus_norvegicus	Mir708	ENSRNOG00000040447

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.