MIR744

Summary

MIR744 (microRNA 744, HGNC:33658) is a microRNA gene on chromosome 17p12.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 100126313 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:33658
Approved symbol	MIR744
Name	microRNA 744
Location	17p12
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-744
Ensembl gene	ENSG00000266297
Ensembl biotype	miRNA
Entrez	100126313
RNAcentral	URS00000EC1BE — miRNA, 98 nt, 23 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000578242

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000578242 — 1 exons

Exon	Start	End
ENSE00003591339	12081899	12081996

Expression profiles

Bgee: expression breadth broad, 99 present calls, max score 81.67.

Top tissues by expression

99 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
blood	UBERON:0000178	81.67	gold quality
bone marrow	UBERON:0002371	81.19	gold quality
monocyte	CL:0000576	77.20	gold quality
leukocyte	CL:0000738	76.23	gold quality
calcaneal tendon	UBERON:0003701	74.61	gold quality
ectocervix	UBERON:0012249	74.27	gold quality
muscle of leg	UBERON:0001383	74.23	gold quality
liver	UBERON:0002107	73.80	gold quality
islet of Langerhans	UBERON:0000006	73.48	gold quality
adrenal tissue	UBERON:0018303	73.46	gold quality
kidney	UBERON:0002113	73.40	gold quality
gastrocnemius	UBERON:0001388	72.94	gold quality
stomach	UBERON:0000945	72.87	gold quality
smooth muscle tissue	UBERON:0001135	71.37	gold quality
body of stomach	UBERON:0001161	70.96	gold quality
body of pancreas	UBERON:0001150	70.92	gold quality
right atrium auricular region	UBERON:0006631	70.69	gold quality
heart	UBERON:0000948	70.67	gold quality
left adrenal gland cortex	UBERON:0035825	70.65	gold quality
corpus callosum	UBERON:0002336	70.43	gold quality
lymph node	UBERON:0000029	70.42	gold quality
urinary bladder	UBERON:0001255	70.38	gold quality
heart left ventricle	UBERON:0002084	70.02	gold quality
tibial artery	UBERON:0007610	69.92	gold quality
saliva-secreting gland	UBERON:0001044	69.86	gold quality
right adrenal gland cortex	UBERON:0035827	69.81	gold quality
tonsil	UBERON:0002372	69.70	gold quality
lower esophagus muscularis layer	UBERON:0035833	69.55	gold quality
adrenal gland	UBERON:0002369	69.51	gold quality
fundus of stomach	UBERON:0001160	69.31	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.56

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 33)

• This work identifies miR-744-directed post-transcriptional regulation of TGF-beta1. (PMID:21991303)
• Our results associate decreased miR-744 expression with HCC recurrence and prognosis, and also suggest that miR-744 is an independent predictor of survival in HCC patients after LT and may therefore be a potential biomarker for their prognosis (PMID:25543521)
• This newly identified miR-744/ARHGAP5 pathway provides further insight into the progression and metastasis of NPC and indicates potential novel therapeutic targets for NPC. (PMID:25961434)
• our data indicate that by targeting PTP1B, miR-744 plays a feed-forward role in regulating type I IFN signaling pathway. (PMID:26259828)
• miR-744 upregulation enhanced the tumorigenicity of pancreatic cancer by activating Wnt/beta-catenin pathway. (PMID:26485754)
• Results showed that high level of plasma miR-744 in pancreatic tumor was correlated with lymph node metastasis and recurrences, poor prognostic factor of patients after pancreatectomy and chemoresistance to gemcitabine in vitro. (PMID:26505678)
• miR-744 targeted on Bcl-2, which leads to the inactivation of apoptosis signaling and the cell proliferation of cervical cancer cells, ameliorating cervical cancer growth and progression. (PMID:27261616)
• Data suggested that miR-744-3p was an oncogenic microRNA in laryngeal squamous cell carcinoma. (PMID:27533461)
• results demonstrated for the first time the regulatory mechanism of miR-744 transcription by c-Jun, providing a potential mechanism underlying the upregulation of miR-744 in cancers (PMID:27533465)
• Data indicate that microRNA miR-744 activated Wnt/beta-catenin pathway by targeting multiple negative regulators of Wnt/beta-catenin signaling, including SFRP1, GSK3beta, TLE3 and NKD1, and that NKD1 is a major functional target of miR-744. (PMID:28107193)
• Low mir744 expression is associated with Breast Cancer. (PMID:28766230)
• Data show that miR-744-5p expression directly downregulated mRNA and protein expression of nuclear factor I X (NFIX) and heterogeneous nuclear ribonucleoprotein C (HNRNPC). (PMID:29899543)
• Reduced exosome miR-425 and miR-744 in the plasma represents the progression of fibrosis and heart failure (PMID:30392569)
• MiR-744-3p regulates the proliferation and differentiation of keratinocytes through targeting KLLN in psoriasis (PMID:30664260)
• LncRNA MAFG-AS1 boosts the proliferation of lung adenocarcinoma cells via regulating miR-744-5p/MAFG axis (PMID:31211984)
• MiR-744 is downregulated in HCC tissues and cell lines as well as in exosomes derived from patient serum and HepG2 cells. Additionally, downregulated miR-744 promotes HepG2 cell proliferation and inhibits the chemosensitivity of HepG2 cells to sorafenib. (PMID:31553714)
• MiR-744 mediates the Oxaliplatin chemoresistance in colorectal cancer through inhibiting BIN1. (PMID:31884801)
• MicroRNA-744 promotes proliferation of osteosarcoma cells by targeting PTEN. (PMID:32186762)
• miR-744-5p mediates lncRNA HOTTIP to regulate the proliferation and apoptosis of papillary thyroid carcinoma cells. (PMID:32335029)
• miR-744-5p contributes to ocular inflammation in patients with primary Sjogrens Syndrome. (PMID:32366870)
• miR-744-5p inhibits cellular proliferation and invasion via targeting ARF1 in epithelial ovarian cancer. (PMID:32558345)
• MicroRNA7445p is downregulated in colorectal cancer and targets SEPT2 to suppress the malignant phenotype. (PMID:33200802)
• Reduction of miR-744 delivered by NSCLC cell-derived extracellular vesicles upregulates SUV39H1 to promote NSCLC progression via activation of the Smad9/BMP9 axis. (PMID:33472665)
• Long noncoding RNA MNX1-AS1 functions as a competing endogenous RNA to regulate epithelial-mesenchymal transition by sponging MiR-744-5p in colorectal cancer. (PMID:33590038)
• miR-744/eNOS/NO axis: A novel target to halt triple negative breast cancer progression. (PMID:33749631)
• LINC01116 promotes the proliferation and invasion of glioma by regulating the microRNA7445pMDM2p53 axis. (PMID:33760190)
• LINC01116 boosts the progression of pituitary adenoma via regulating miR-744-5p/HOXB8 pathway. (PMID:34098015)
• Analysis of the Applicability of microRNAs in Peripheral Blood Leukocytes as Biomarkers of Sensitivity and Exposure to Fractionated Radiotherapy towards Breast Cancer. (PMID:34445424)
• MiR-744 Functions as an Oncogene Through Direct Binding to c-Fos Promoter and Facilitates Non-small Cell Lung Cancer Progression. (PMID:34599436)
• LncRNA MNX1-AS1 promotes ovarian cancer process via targeting the miR-744-5p/SOX12 axis. (PMID:34789303)
• Mesenchymal stem cell-derived extracellular vesicles prevent glioma by blocking M2 polarization of macrophages through a miR-744-5p/TGFB1-dependent mechanism. (PMID:34978010)
• lncRNA TM4SF1-AS1 predicts dismal outcomes and promotes cholangiocarcinoma progression via modulating miR-744-3p. (PMID:35346892)
• miR-744-5p promotes T-cell differentiation via inhibiting STK11. (PMID:38830518)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir744	ENSMUSG00000076460

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.