MIR99B

Summary

MIR99B (microRNA 99b, HGNC:31651) is a microRNA gene on chromosome 19q13.41.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 407056 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31651
Approved symbol	MIR99B
Name	microRNA 99b
Location	19q13.41
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-99b
Ensembl gene	ENSG00000207550
Ensembl biotype	miRNA
OMIM	614510
Entrez	407056
RNAcentral	URS000075E904 — miRNA, 70 nt, 39 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000384819

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000384819 — 1 exons

Exon	Start	End
ENSE00001499826	51692612	51692681

Expression profiles

Bgee: expression breadth broad, 81 present calls, max score 98.40.

Top tissues by expression

81 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
sural nerve	UBERON:0015488	98.40	gold quality
renal glomerulus	UBERON:0000074	81.38	gold quality
blood	UBERON:0000178	76.40	gold quality
placenta	UBERON:0001987	76.25	gold quality
prefrontal cortex	UBERON:0000451	75.81	gold quality
muscle of leg	UBERON:0001383	74.20	gold quality
epididymis	UBERON:0001301	74.12	gold quality
gall bladder	UBERON:0002110	73.77	gold quality
gastrocnemius	UBERON:0001388	73.18	gold quality
heart left ventricle	UBERON:0002084	72.55	gold quality
heart	UBERON:0000948	71.18	gold quality
omental fat pad	UBERON:0010414	70.18	gold quality
endometrium	UBERON:0001295	69.93	gold quality
right atrium auricular region	UBERON:0006631	69.36	gold quality
frontal lobe	UBERON:0016525	68.85	gold quality
adipose tissue	UBERON:0001013	68.61	gold quality
body of stomach	UBERON:0001161	68.37	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	68.36	gold quality
caudate nucleus	UBERON:0001873	68.17	gold quality
anterior cingulate cortex	UBERON:0009835	67.90	gold quality
ascending aorta	UBERON:0001496	67.68	gold quality
right frontal lobe	UBERON:0002810	67.62	gold quality
left adrenal gland cortex	UBERON:0035825	67.45	gold quality
Ammon’s horn	UBERON:0001954	67.43	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	67.05	gold quality
thoracic aorta	UBERON:0001515	66.93	gold quality
right ovary	UBERON:0002118	66.69	gold quality
right lobe of thyroid gland	UBERON:0001119	66.62	gold quality
lower esophagus muscularis layer	UBERON:0035833	66.55	gold quality
adult mammalian kidney	UBERON:0000082	66.47	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.21

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 29)

• 3 targets of miR-99 family (miR-99a, -99b, -100) were SMARCA5, SMARCD1, mTOR; PSA is regulated by miR-99 family, at least partially, by repression of SMARCA5; suggests key functions and targets of miR-99 family in prostate cancer suppression and prognosis (PMID:21212412)
• miR-99b, -181a, and -181b comprise a component of an endothelial-miRNA signature and are capable of potentiating endothelial cell differentiation from pluripotent human embryonic stem cells. (PMID:22232059)
• members of miR-99 family (miR-99a, miR-99b, miR-100) were expressed during dermal wound healing. (PMID:23724047)
• Ionizing radiation promoted mTOR expression and activation in pancreatic cancer cells through reducing miR-99b expression, which negatively regulated mTOR. (PMID:23886294)
• Low miR-99b expression is associated with cervical cancer. (PMID:24668416)
• the expression of miR-99b was inversely correlated with CLDN11 levels . These findings suggest that a high level of miR-99b expression is an independent prognostic factor and correlates with poor survival of patients with Hepatocellular carcinoma (HCC) (PMID:26134929)
• miR-99b-5p is differently expressed in primary colorectal cancer (CRC) and liver metastasis and functions as a tumor-suppressive microRNA in metastatic CRC (PMID:26259252)
• Downregulation of miR-99b with concomitant upregulation of its target gene IGF-1R may over-induce the PI3K-AKT signaling pathway, leading to deregulated cell proliferation in Condyloma acuminatum. (PMID:26297545)
• Low miR-99b-5p expression analyzed with sequencing methodology may correlate with tumor progression in tyrosine kinase inhibitorI-treated clear cell renal cell carcinoma patients patients. (PMID:27738339)
• ARID3A was down-regulated by ZEB1 in a miR-99b/let-7e/miR-125a dependent mannerto promotes invasion and metastasis in esophageal squamous cell carcinoma. (PMID:28408353)
• MiR-99b targets IGF-1R and functions as a tumor suppressor in gastric cancer. (PMID:29973668)
• our findings identified the miR-125a99blet-7e cluster as a new key intracellular mediator used by the master anti-inflammatory cytokine IL-10 to dampen down the pro-inflammatory TLR pathway and provides a new perspective in the understanding of mechanisms by which the inflammatory response is regulated in phagocytes. (PMID:30245693)
• Fractions enriched in nanosized RNAcarriers were separated from the plasma of young participants in this study with Down syndrome and their nontrisomic siblings and miRNAs were extracted. A microarraybased analysis on a small cohort of samples led to the identification of the three most abundant miRNAs, namely miR165p, miR99b5p and miR1443p. (PMID:31017260)
• Circulating MiR-99b-5p, in particular, may serve as a possible predictor for erosion progression in early rheumatoid arthritis. (PMID:31273939)
• Identification of PBMC-expressed miRNAs for rheumatoid arthritis. (PMID:31588838)
• aberrantly expressed miR-99b serves a prognostic biomarker for OS patients (PMID:31651329)
• Plasma level of miR-99b may serve as potential diagnostic and short-term prognostic markers in patients with acute cerebral infarction. (PMID:31967688)
• MiR-99b regulates cerebral ischemia neuronal injury through targeting IGF1R. (PMID:32343508)
• MicroRNA-99b-5p downregulates protein synthesis in human primary myotubes. (PMID:32608991)
• MicroRNA-99b inhibits NSCLC cell invasion and migration by directly targeting NIPBL. (PMID:33660799)
• MFG-E8 regulated by miR-99b-5p protects against osteoarthritis by targeting chondrocyte senescence and macrophage reprogramming via the NF-kappaB pathway. (PMID:34031369)
• MiR-99b-5p suppressed proliferation of human osteoblasts by targeting FGFR3 in osteoporosis. (PMID:34156640)
• Identification of miR-199a-5p, miR-214-3p and miR-99b-5p as Fibrosis-Specific Extracellular Biomarkers and Promoters of HSC Activation. (PMID:34575957)
• Human bone marrow mesenchymal stem cells-derived exosomes attenuated prostate cancer progression via the miR-99b-5p/IGF1R axis. (PMID:35030978)
• MicroRNA-99b-5p targets mTOR/AR axis, induces autophagy and inhibits prostate cancer cell proliferation. (PMID:35811549)
• Human endothelial cell-derived exosomal microRNA-99a/b drives a sustained inflammatory response during sepsis by inhibiting mTOR expression. (PMID:36061862)
• Downregulation of miR-99b-5p and Upregulation of Nuclear mTOR Cooperatively Promotes the Tumor Aggressiveness and Drug Resistance in African American Prostate Cancer. (PMID:36077039)
• Effect of the MiR-99b and MiR-135b on peritoneal carcinomatosis and liver metastasis in colorectal cancer. (PMID:37639911)
• Drug-resistant exosome miR-99b-3p induces macrophage polarization and confers chemoresistance on sensitive cells by targeting PPP2CA. (PMID:39298813)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir99b	ENSMUSG00000065396
rattus_norvegicus	Mir99b	ENSRNOG00000035654

Paralogs (7): MIR99A (ENSG00000207638), MIR10B (ENSG00000207744), MIR125B2 (ENSG00000207863), MIR125B1 (ENSG00000207971), MIR100 (ENSG00000207994), MIR125A (ENSG00000208008), MIR10A (ENSG00000284038)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.