MIRLET7I

Summary

MIRLET7I (microRNA let-7i, HGNC:31486) is a microRNA gene on chromosome 12q14.1.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406891 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31486
Approved symbol	MIRLET7I
Name	microRNA let-7i
Location	12q14.1
Locus type	RNA, micro
Status	Approved
Aliases	hsa-let-7i
Ensembl gene	ENSG00000199179
Ensembl biotype	miRNA
OMIM	612148
Entrez	406891
RNAcentral	URS000075B982 — miRNA, 84 nt, 25 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362309

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362309 — 1 exons

Exon	Start	End
ENSE00001437072	62603686	62603769

Expression profiles

Bgee: expression breadth broad, 97 present calls, max score 83.21.

Top tissues by expression

97 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
calcaneal tendon	UBERON:0003701	83.21	gold quality
bone marrow	UBERON:0002371	80.39	gold quality
granulocyte	CL:0000094	78.84	gold quality
placenta	UBERON:0001987	78.34	gold quality
blood	UBERON:0000178	77.57	gold quality
adrenal tissue	UBERON:0018303	77.33	gold quality
renal glomerulus	UBERON:0000074	76.72	gold quality
apex of heart	UBERON:0002098	76.65	gold quality
muscle of leg	UBERON:0001383	75.65	gold quality
adult mammalian kidney	UBERON:0000082	75.47	gold quality
endometrium	UBERON:0001295	75.42	gold quality
monocyte	CL:0000576	74.44	gold quality
gastrocnemius	UBERON:0001388	72.82	gold quality
islet of Langerhans	UBERON:0000006	72.69	gold quality
adenohypophysis	UBERON:0002196	72.55	gold quality
kidney	UBERON:0002113	72.37	gold quality
uterus	UBERON:0000995	71.88	gold quality
left lobe of thyroid gland	UBERON:0001120	71.73	gold quality
heart left ventricle	UBERON:0002084	71.61	gold quality
heart	UBERON:0000948	70.83	gold quality
thyroid gland	UBERON:0002046	70.79	gold quality
right lobe of thyroid gland	UBERON:0001119	70.74	gold quality
fundus of stomach	UBERON:0001160	70.74	gold quality
right atrium auricular region	UBERON:0006631	70.16	gold quality
right lobe of liver	UBERON:0001114	70.13	gold quality
myometrium	UBERON:0001296	69.77	gold quality
stomach	UBERON:0000945	69.69	gold quality
body of stomach	UBERON:0001161	69.27	gold quality
omental fat pad	UBERON:0010414	69.22	gold quality
left adrenal gland	UBERON:0001234	69.14	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.79

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• Microbial infection promotes the formation of a NFkappaB p50-C/EBPbeta silencer complex in the regulatory sequence of microRNA let-7i. (PMID:19903813)
• let-7i is associated with colorectal cancer metastasis. (PMID:21625861)
• Tristetraprolin promotes an increase in expression of mature let-7, which leads to the inhibition of let-7 target gene CDC34 expression and suppresses cell growth. (PMID:22210895)
• low let-7i expression was an unfavorable prognostic factor of overall survival in gastric cancer. (PMID:23107361)
• In the HNSCC cell line OECM-1, knockdown of BMP4 reduced mesenchymal-mode migration and invasion in 3D culture. In clinical HNSCC samples, let-7i expression was inversely correlated with BMP4 expression. (PMID:23454123)
• Taenia crassiceps antigens repress LPS-induced Let-7i but not miR-146a and miR-155 in human dendritic cells (PMID:23509825)
• Expression of let-7i correlated positively with the Bath Ankylosing Spondylitis Radiology Index of lumbar spine in AS patients. It facilitated the T helper type 1 (IFN-gamma) immune response in T cells. (PMID:23607629)
• Findings demonstrate that repression of let-7i expression by mutant p53 has a key role in enhancing migration, invasion and metastasis. (PMID:24662829)
• Cryptosporidium parvum induces SIRT1 expression in host epithelial cells through downregulating let-7i. (PMID:24862934)
• Expression of SUMO1/2/3 is dramatically enhanced by interferons through an miRNA-based mechanism involving the Lin28/let-7. (PMID:24942926)
• Our data indicated that let-7i might control T cells fates in AS by targeting IGF1R. (PMID:25305490)
• our results indicate that changes in serum miRNAs are associated with cigarette smoking and lung cancer and that let-7i-3p and miR-154-5p are potential biomarkers of smoking-related lung cancer. (PMID:25386559)
• Loss of let-7i expression is associated with chemoresistance in ovarian cancer. (PMID:25451316)
• The above-mentioned data suggest that let-7b/i inhibit the invasive ability of glioma cells by directly downregulating IKBKE and indirectly upregulating E-cadherin. (PMID:25656572)
• Enhanced let-7i expression suppresses cell migration and invasion of osteosarcoma (OS) cells in vitro. Let-7i inhibits OS cell malignant phenotype partly by targeting Aurora-B. (PMID:25997616)
• genetic association study in population in Beijing, China: Data suggest 3 SNPs in mirnlet7 (rs10993081 mirnlet7d, rs10877887 mirnlet7i, rs17276588 mirnlet7f) on chromosomes 9, 12, and x are associated with metabolic syndrome in the population studied. (PMID:26178671)
• Overexpression or knockdown of let-7i in cultured cardiac fibroblasts demonstrated that let-7i played an inhibitory effect on the expression of its targets interleukin-6 and collagens. (PMID:26259595)
• BAG3-mediated miRNA let-7g and let-7i inhibit proliferation and enhance apoptosis of human esophageal carcinoma cells by targeting the drug transporter ABCC10 and modulates cisplatin resistance. (PMID:26655271)
• let-7i may control HCC tumorigenesis by regulating IGF1R directly and indirectly by interrupting the interplay between IGF1R and the IGF2BPs. (PMID:27126374)
• Data reported that let-7i upregulates IL-2 expression by targeting the promoter TATA-box region, which functions as a positive regulator. In HIV-1 infection, the expression of let-7i in CD4(+) T cells is decreased by attenuating its promoter activity. The reduced let-7i miRNA expression led to a decline in IL-2 levels which contributes to CD4(+) T cells death. (PMID:27145859)
• MIRlet-7i is able to regulate autophagic activity via regulating Atg4B expression, which might contribute to the pathogenesis of pre-eclampsia. (PMID:27770612)
• Study found that Let7i is decreased in circulating leukocytes of patients with acute ischemic stroke. Mechanisms by which let7i regulates inflammatory response post stroke include targeting CD86, CXCL8, and HMGB1. (PMID:27784773)
• study revealed a molecular pathway consisting of BMI1, miRNA let-7i, and ERK3, which controls the migration of head and neck cancer cells, and suggests that ERK3 kinase is a potential new therapeutic target in head and neck cancers, particularly those with BMI1 overexpression. (PMID:28079973)
• Using RNA of isolated chromatin combined with massive parallel sequencing (RICh-seq) we show that let-7i miRNA suppresses NET by methyl-CpG-binding protein 2 (MeCP2). (PMID:28352654)
• let-7i directly regulates expression of ST3GAL IV gene chronic myeloid leukemia cells. (PMID:28512058)
• Our research suggests that let-7i promotes OGD-induced inflammation via downregulating TLR4 expression. (PMID:28844675)
• The circulating miRNAs let-7i and miR-15a showed differential expression between AIS patients receiving thrombolysis treatment (rt-PA group) and those not receiving it (non-rt-PA group). Compared to the non-rt-PA group, the rt-PA group showed upregulation of let-7i and downregulation of miR-15a. (PMID:28892656)
• Data indicate that microRNA let-7i (let-7i) is markedly increased in patients with multiple sclerosis (MS). (PMID:29295981)
• In the risk study, the let-7i rs10877887 and let-7a-1/let-7f-1/let-7d rs13293512 were shown no significant association for the overall cancer risk. In the stratified analysis, the rs10877887 variant genotype was significantly associated with a decreased cancer risk in head and neck cancer. (PMID:29717029)
• The study provides strong evidence of suppressive effects of let-7i on gastric cancer development and progression. (PMID:29858755)
• Low LET-7I expression is associated with metastasis of colon cancer. (PMID:30015976)
• Plasma let-7i levels may serve as a biomarker for the diagnosis of ankylosing spondylitis in Mexican individuals (PMID:30911942)
• Propofol promotes apoptosis of colorectal cancer cells via alleviating the suppression of lncRNA HOXA11-AS on miRNA let-7i. (PMID:31013434)
• Let-7i-3p, acting as a negative regulator of the Wnt/beta-catenin pathway by targeting LEF1, inhibits the osteogenic differentiation of hASCs under cyclic strain in vitro. (PMID:31753039)
• Let-7i is down-regulated in drug-resistant breast cancer cells, and negatively correlated with chemotherapy resistance. (PMID:31878995)
• Study suggested that the expression levels of plasma let-7i were associated with the development of left ventricular remodeling. (PMID:31885737)
• Silencing TTTY15 mitigates hypoxia-induced mitochondrial energy metabolism dysfunction and cardiomyocytes apoptosis via TTTY15/let-7i-5p and TLR3/NF-kappaB pathways. (PMID:32926961)
• Extracellular vesicles-encapsulated let-7i shed from bone mesenchymal stem cells suppress lung cancer via KDM3A/DCLK1/FXYD3 axis. (PMID:33350586)
• microRNA let-7i-5p mediates the relationship between muscle fat infiltration and neck pain disability following motor vehicle collision: a preliminary study. (PMID:33542428)
• Let-7i-5p enhances cell proliferation, migration and invasion of ccRCC by targeting HABP4. (PMID:33775245)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	dre-let-7i	ENSDARG00000082738
mus_musculus	Mirlet7i	ENSMUSG00000065406
drosophila_melanogaster	mir-let7	FBGN0262406

Paralogs (10): MIRLET7E (ENSG00000198972), MIRLET7A2 (ENSG00000198975), MIRLET7C (ENSG00000199030), MIRLET7F1 (ENSG00000199072), MIRLET7D (ENSG00000199133), MIRLET7G (ENSG00000199150), MIRLET7A1 (ENSG00000199165), MIRLET7F2 (ENSG00000208012), MIR98 (ENSG00000271886), MIRLET7A3 (ENSG00000283990)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.