MISP

Summary

MISP (mitotic spindle positioning, HGNC:27000) is a protein-coding gene on chromosome 19p13.3, encoding Mitotic interactor and substrate of PLK1 (Q8IVT2). Plays a role in mitotic spindle orientation and mitotic progression.

The protein encoded by this gene is an actin-bundling protein involved in determining cell morphology and mitotic progression. The encoded protein is required for the proper positioning of the mitotic spindle. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.

Source: NCBI Gene 126353 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 224 total
• Druggable target: yes
• MANE Select transcript: NM_173481

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:27000
Approved symbol	MISP
Name	mitotic spindle positioning
Location	19p13.3
Locus type	gene with protein product
Status	Approved
Aliases	DKFZp686H18209, Caprice, MISP1
Ensembl gene	ENSG00000099812
Ensembl biotype	protein_coding
OMIM	615289
Entrez	126353

Gene structure

Transcript identifiers

Ensembl transcripts: 22 — 21 protein_coding, 1 protein_coding_CDS_not_defined

ENST00000215582, ENST00000614180, ENST00000871265, ENST00000871266, ENST00000871267, ENST00000871268, ENST00000871269, ENST00000871270, ENST00000871271, ENST00000871272, ENST00000871273, ENST00000871274, ENST00000871275, ENST00000871276, ENST00000871277, ENST00000871278, ENST00000871279, ENST00000871280, ENST00000871281, ENST00000871282, ENST00000871283, ENST00000871284

RefSeq mRNA: 1 — MANE Select: NM_173481 NM_173481

CCDS: CCDS12042

Canonical transcript exons

ENST00000215582 — 5 exons

Exon	Start	End
ENSE00000655141	759909	760039
ENSE00000655143	756890	758726
ENSE00000892413	761625	761663
ENSE00001311215	763501	764318
ENSE00001410788	751112	751171

Expression profiles

Bgee: expression breadth ubiquitous, 166 present calls, max score 99.00.

FANTOM5 (CAGE): breadth broad, TPM avg 6.4034 / max 134.0150, expressed in 348 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
172746	3.6109	318
172745	2.6957	313
208622	0.0969	73

Top tissues by expression

266 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
ileal mucosa	UBERON:0000331	99.00	gold quality
mucosa of transverse colon	UBERON:0004991	98.66	gold quality
colonic mucosa	UBERON:0000317	97.01	gold quality
duodenum	UBERON:0002114	96.88	gold quality
jejunal mucosa	UBERON:0000399	96.68	gold quality
mucosa of sigmoid colon	UBERON:0004993	96.61	gold quality
nasal cavity epithelium	UBERON:0005384	93.86	gold quality
rectum	UBERON:0001052	93.38	gold quality
small intestine	UBERON:0002108	90.91	gold quality
endometrium epithelium	UBERON:0004811	90.58	gold quality
small intestine Peyer’s patch	UBERON:0003454	90.55	gold quality
olfactory bulb	UBERON:0002264	89.75	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	89.34	gold quality
type B pancreatic cell	CL:0000169	89.11	silver quality
transverse colon	UBERON:0001157	88.71	gold quality
jejunum	UBERON:0002115	88.59	gold quality
mucosa of paranasal sinus	UBERON:0005030	87.74	gold quality
epithelium of bronchus	UBERON:0002031	87.56	gold quality
bronchus	UBERON:0002185	87.25	gold quality
palpebral conjunctiva	UBERON:0001812	85.89	gold quality
bronchial epithelial cell	CL:0002328	85.78	gold quality
right uterine tube	UBERON:0001302	85.68	gold quality
amniotic fluid	UBERON:0000173	83.27	gold quality
tongue squamous epithelium	UBERON:0006919	83.09	silver quality
gall bladder	UBERON:0002110	83.04	gold quality
diaphragm	UBERON:0001103	82.94	gold quality
nasal cavity mucosa	UBERON:0001826	82.68	gold quality
epithelial cell of pancreas	CL:0000083	81.99	silver quality
caecum	UBERON:0001153	81.80	gold quality
vermiform appendix	UBERON:0001154	81.17	gold quality

Single-cell (SCXA)

Detected in 5 experiment(s), a significant marker in 3.

Experiment	Marker?	Max mean expression
E-GEOD-125970	yes	70.34
E-MTAB-8410	yes	22.65
E-GEOD-86618	no	153.66
E-MTAB-6386	no	2.97
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

32 targeting MISP, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4713-3P	100.00	65.92	505
HSA-MIR-4267	99.96	66.53	2368
HSA-MIR-182-5P	99.87	74.03	2589
HSA-MIR-629-3P	99.85	67.99	1875
HSA-MIR-4447	99.85	67.81	2900
HSA-MIR-6842-5P	99.80	67.54	1587
HSA-MIR-7110-5P	99.80	67.84	1712
HSA-MIR-4713-5P	99.78	67.80	1794
HSA-MIR-6764-5P	99.75	67.89	2304
HSA-MIR-92A-2-5P	99.75	67.01	2164
HSA-MIR-4470	99.66	69.35	1767
HSA-MIR-1915-3P	99.58	66.79	1988
HSA-MIR-4761-5P	99.51	66.69	804
HSA-MIR-4641	99.28	66.64	744
HSA-MIR-4279	99.19	66.70	2437
HSA-MIR-6846-5P	98.81	65.86	1121
HSA-MIR-6848-5P	98.81	65.49	1126
HSA-MIR-4726-3P	98.49	63.89	1385
HSA-MIR-6864-5P	98.38	66.59	1079
HSA-MIR-4436B-3P	98.25	65.26	1494
HSA-MIR-6735-5P	98.24	65.36	1488
HSA-MIR-7843-5P	98.12	65.26	1421
HSA-MIR-4494	97.86	64.93	850
HSA-MIR-4632-5P	97.82	65.38	1470
HSA-MIR-6879-5P	97.77	65.52	1521
HSA-MIR-3127-5P	97.52	65.24	786
HSA-MIR-3622A-3P	97.06	66.43	1000
HSA-MIR-6729-3P	96.91	66.79	703
HSA-MIR-3622B-3P	96.82	66.36	988
HSA-MIR-203A-5P	96.33	65.03	714

Literature-anchored findings (GeneRIF, showing 10)

• Plk1 phosphorylates MISP, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning. (PMID:23509069)
• MISP links microtubules to the actin cytoskeleton and focal adhesions in order to properly position the mitotic spindle. (PMID:23574715)
• Bundles F-actin and balances stress fiber-filopodial formation (PMID:24475924)
• MISP is an effector for cell type-specific actin reorganization with its direct actin-binding properties and provides a novel model of cell morphology regulation by a non-ubiquitous single actin-bundling protein. (PMID:24475924)
• MISP directly interacts with ezrin and that SLK/LOK-activated ezrin ensures appropriate cortical MISP levels in mitosis by competing with MISP for actin-binding sites at the cell cortex. (PMID:29669740)
• Modulation of actin-binding and -bundling activities of MISP/Caprice by multiple phosphorylation. (PMID:34023777)
• PLK1 and its substrate MISP facilitate intrahepatic cholangiocarcinoma progression by promoting lymphatic invasion and impairing E-cadherin adherens junctions. (PMID:38057358)
• Plants distinguish different photoperiods to independently control seasonal flowering and growth. (PMID:38330117)
• Mitotic spindle positioning protein (MISP) preferentially binds to aged F-actin. (PMID:38588808)
• MISP Is Overexpressed in Intestinal Metaplasia and Gastric Cancer. (PMID:38785491)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Misp	ENSMUSG00000035852
rattus_norvegicus	Misp	ENSRNOG00000010423

Paralogs (1): MISP3 (ENSG00000141854)

Protein

Protein identifiers

Mitotic interactor and substrate of PLK1 — Q8IVT2 (reviewed: Q8IVT2)

Alternative names: Mitotic spindle positioning protein

All UniProt accessions (1): Q8IVT2

UniProt curated annotations — full annotation on UniProt →

Function. Plays a role in mitotic spindle orientation and mitotic progression. Regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning. May link microtubules to the actin cytospkeleton and focal adhesions. May be required for directed cell migration and centrosome orientation. May also be necessary for proper stacking of the Golgi apparatus.

Subunit / interactions. Associates with F-actin. Interacts with DCTN1; this interaction regulates DCTN1 distribution at the cell cortex. Interacts with PTK2/FAK and MAPRE1.

Subcellular location. Cell junction. Focal adhesion. Cytoplasm. Cytoskeleton. Cell cortex.

Post-translational modifications. Phosphorylated by CDK1 and PLK1. CDK1 is the priming kinase for PLK1 phosphorylation. Phosphorylation by PLK1 is required for proper spindle orientation at metaphase.

Similarity. Belongs to the MISP family.

RefSeq proteins (1): NP_775752* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR029304	AKAP2_C	Domain
IPR042779	MISP/MISP3-like	Family

Pfam: PF15304

UniProt features (67 total): modified residue 26, mutagenesis site 22, region of interest 6, compositionally biased region 6, sequence variant 5, chain 1, coiled-coil region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8IVT2-F1	54.42	0.07

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (26): 78, 156, 164, 172, 179, 214, 219, 224, 284, 287, 348, 377, 382, 394, 395, 397, 400, 430, 471, 541 …

Mutagenesis-validated functional residues (22):

Position	Phenotype
78	almost complete loss of cdk1 phosphorylation in vitro, loss of plk1-binding, no effect on cortical localization; when as
164	almost complete loss of cdk1 phosphorylation in vitro, loss of plk1-binding, no effect on cortical localization; when as
172	almost complete loss of cdk1 phosphorylation in vitro, loss of plk1-binding, no effect on cortical localization; when as
214	almost complete loss of cdk1 phosphorylation in vitro, loss of plk1-binding, no effect on cortical localization; when as
224	almost complete loss of cdk1 phosphorylation in vitro, loss of plk1-binding, no effect on cortical localization; when as
284	almost complete loss of cdk1 phosphorylation in vitro, loss of plk1-binding, no effect on cortical localization; when as
287	almost complete loss of cdk1 phosphorylation in vitro, loss of plk1-binding, no effect on cortical localization; when as
377	almost complete loss of cdk1 phosphorylation in vitro, loss of plk1-binding, no effect on cortical localization; when as
382	almost complete loss of cdk1 phosphorylation in vitro, loss of plk1-binding, no effect on cortical localization; when as
394	drastic reduction in plk1 phosphorylation in vitro, no effect on cortical localization; when associated with a-382; a-39
394	no effect on cortical localization; when associated with d-395; d-397; d-471; d-582 and d-586.
395	drastic reduction in plk1 phosphorylation in vitro, no effect on cortical localization; when associated with a-382; a-39
395	no effect on cortical localization; when associated with d-394; d-397; d-471; d-582 and d-586.
397	drastic reduction in plk1 phosphorylation in vitro, no effect on cortical localization; when associated with a-382; a-39
397	no effect on cortical localization; when associated with d-394; d-395; d-471; d-582 and d-586.
471	drastic reduction in plk1 phosphorylation in vitro, no effect on cortical localization; when associated with a-382; a-39
471	no effect on cortical localization; when associated with d-394; d-395; d-397; d-582 and d-586.
575	almost complete loss of cdk1 phosphorylation in vitro, loss of plk1-binding, no effect on cortical localization; when as
582	drastic reduction in plk1 phosphorylation in vitro, no effect on cortical localization; when associated with a-382; a-39
582	no effect on cortical localization; when associated with d-394; d-395; d-397; d-471 and d-586.
586	drastic reduction in plk1 phosphorylation in vitro, no effect on cortical localization; when associated with a-382; a-39
586	no effect on cortical localization; when associated with d-394; d-395; d-397; d-471 and d-582.

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 194 (showing top): GOBP_CHROMOSOME_ORGANIZATION, GOBP_ESTABLISHMENT_OF_SPINDLE_ORIENTATION, GOBP_CYTOPLASMIC_MICROTUBULE_ORGANIZATION, GOBP_ESTABLISHMENT_OR_MAINTENANCE_OF_CELL_POLARITY, GOBP_SPINDLE_LOCALIZATION, GGAMTNNNNNTCCY_UNKNOWN, CHANDRAN_METASTASIS_DN, GOCC_MICROTUBULE_ORGANIZING_CENTER, GOBP_ESTABLISHMENT_OF_CELL_POLARITY, MARTINEZ_RB1_TARGETS_UP, GOBP_MITOTIC_SPINDLE_ASSEMBLY, GOBP_ORGANELLE_FISSION, GOCC_CENTROSOME, GOBP_MITOTIC_NUCLEAR_DIVISION, GOBP_ORGANELLE_ASSEMBLY

GO Biological Process (9): establishment of mitotic spindle orientation (GO:0000132), cell migration (GO:0016477), cortical actin cytoskeleton organization (GO:0030866), astral microtubule organization (GO:0030953), cell division (GO:0051301), organelle localization (GO:0051640), establishment of centrosome localization (GO:0051660), mitotic spindle assembly (GO:0090307), regulation of protein localization to cell cortex (GO:1904776)

GO Molecular Function (3): actin filament binding (GO:0051015), actin binding (GO:0003779), protein binding (GO:0005515)

GO Cellular Component (10): actin filament (GO:0005884), plasma membrane (GO:0005886), focal adhesion (GO:0005925), cell cortex (GO:0005938), spindle pole centrosome (GO:0031616), cytoplasm (GO:0005737), cytoskeleton (GO:0005856), cortical actin cytoskeleton (GO:0030864), anchoring junction (GO:0070161), mitotic spindle astral microtubule end (GO:1905721)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
actin cytoskeleton	2
cell periphery	2
mitotic cell cycle	1
establishment of mitotic spindle localization	1
establishment of spindle orientation	1
cell motility	1
actin cytoskeleton organization	1
cortical cytoskeleton organization	1
spindle organization	1
cytoplasmic microtubule organization	1
cellular process	1
localization	1
centrosome localization	1
establishment of localization in cell	1
establishment of organelle localization	1
mitotic sister chromatid segregation	1
mitotic spindle organization	1
spindle assembly	1
mitotic nuclear division	1
protein localization to cell cortex	1
regulation of protein localization to cell periphery	1
actin binding	1
protein-containing complex binding	1
cytoskeletal protein binding	1
binding	1
polymeric cytoskeletal fiber	1
membrane	1
cell-substrate junction	1
cytoplasm	1
spindle pole	1
centrosome	1
intracellular anatomical structure	1
cellular anatomical structure	1
intracellular membraneless organelle	1
cortical cytoskeleton	1
cell junction	1
mitotic spindle astral microtubule	1
microtubule end	1

Protein interactions and networks

STRING

570 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
MISP	F8W876	F8W876	720
MISP	TULP4	Q9NRJ4	720
MISP	MASP2	O00187	623
MISP	MISP3	Q96FF7	599
MISP	PALM2AKAP2	Q9Y2D5	571
MISP	MYBL2	P10244	564
MISP	SAPCD2	Q86UD0	511
MISP	CDK1	P06493	457
MISP	PDZD11	Q5EBL8	446
MISP	PPP1R12A	O14974	443
MISP	NUMA1	Q14980	370
MISP	MIPEP	Q99797	355
MISP	CCT8	P50990	354
MISP	R3HDM4	Q96D70	348
MISP	FAXC	Q5TGI0	343

IntAct

273 interactions, top by confidence:

A	B	Type	Score
EZR	MISP	psi-mi:“MI:0915”(physical association)	0.740
MISP	BOLL	psi-mi:“MI:0915”(physical association)	0.560
MISP	SF3A2	psi-mi:“MI:0915”(physical association)	0.560
MISP	ZNF774	psi-mi:“MI:0915”(physical association)	0.560
MISP	PRR5L	psi-mi:“MI:0915”(physical association)	0.560
MISP	MAP1LC3C	psi-mi:“MI:0915”(physical association)	0.560
MISP	ZNF620	psi-mi:“MI:0915”(physical association)	0.560
MISP	BLZF1	psi-mi:“MI:0915”(physical association)	0.560
TRAF2	MISP	psi-mi:“MI:0915”(physical association)	0.560
MISP	IKZF3	psi-mi:“MI:0915”(physical association)	0.560
GOLGA2	MISP	psi-mi:“MI:0915”(physical association)	0.560
MISP	PIH1D2	psi-mi:“MI:0915”(physical association)	0.560
TERF1	MISP	psi-mi:“MI:0915”(physical association)	0.560
SF3A2	MISP	psi-mi:“MI:0915”(physical association)	0.560
ZNF774	MISP	psi-mi:“MI:0915”(physical association)	0.560
MISP	PNMA2	psi-mi:“MI:0915”(physical association)	0.560
LMO1	MISP	psi-mi:“MI:0915”(physical association)	0.560
TRIP6	MISP	psi-mi:“MI:0915”(physical association)	0.560
MISP	CEP76	psi-mi:“MI:0915”(physical association)	0.560
HSF2BP	MISP	psi-mi:“MI:0915”(physical association)	0.560
KRTAP12-3	MISP	psi-mi:“MI:0915”(physical association)	0.560
GPRASP3	MISP	psi-mi:“MI:0915”(physical association)	0.560
HNRNPK	MISP	psi-mi:“MI:0915”(physical association)	0.560
EFHC2	MISP	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (224): MISP (Affinity Capture-MS), MISP (Affinity Capture-MS), MISP (Affinity Capture-MS), MISP (Affinity Capture-MS), MISP (Affinity Capture-MS), MISP (Affinity Capture-MS), MISP (Affinity Capture-MS), MISP (Affinity Capture-MS), MISP (Affinity Capture-MS), MISP (Affinity Capture-MS), MISP (Affinity Capture-MS), MISP (Affinity Capture-MS), MISP (Affinity Capture-MS), MISP (Affinity Capture-MS), MISP (Affinity Capture-MS)

ESM2 similar proteins: A2A7S8, A2AI08, A5D7K1, B0BN13, O54931, O75128, Q13796, Q2NL68, Q32LQ1, Q3U2K0, Q499V8, Q4KMQ1, Q5JTD0, Q5JXC2, Q5NBX1, Q5RBH3, Q5SW24, Q5SX79, Q5T0Z8, Q5XHX2, Q68DK7, Q6P9J5, Q6PDH0, Q6PDM1, Q6PFX7, Q7TN08, Q7TT28, Q7Z591, Q86WR7, Q8BG26, Q8BI29, Q8BRV5, Q8C5R2, Q8CC35, Q8CCJ4, Q8IVT2, Q8IY92, Q8K124, Q8N3V7, Q8N7J2

Diamond homologs: Q5RBH3, Q8IVT2, Q96FF7, Q9D279

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 100 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Sensory processing of sound by inner hair cells of the cochlea	5	14.1×	2e-03
RHO GTPase Effectors	7	8.2×	2e-03
Signaling by Rho GTPases, Miro GTPases and RHOBTB3	9	5.2×	2e-03
Signaling by Rho GTPases	8	4.7×	7e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

224 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	186
Likely benign	16
Benign	9

Top pathogenic / likely-pathogenic (0)

SpliceAI

634 predictions. Top by Δscore:

Variant	Effect	Δscore
19:756888:A:AG	acceptor_gain	1.0000
19:756889:G:GG	acceptor_gain	1.0000
19:756889:GTAA:G	acceptor_gain	1.0000
19:759906:CAGG:C	acceptor_loss	1.0000
19:759907:A:AG	acceptor_gain	1.0000
19:759907:A:AT	acceptor_loss	1.0000
19:759907:AG:A	acceptor_gain	1.0000
19:759908:G:GG	acceptor_gain	1.0000
19:759908:GG:G	acceptor_gain	1.0000
19:759908:GGC:G	acceptor_gain	1.0000
19:759908:GGCA:G	acceptor_gain	1.0000
19:759908:GGCAT:G	acceptor_gain	1.0000
19:760038:GG:G	donor_gain	1.0000
19:760038:GGGTG:G	donor_loss	1.0000
19:760039:GG:G	donor_gain	1.0000
19:760039:GGT:G	donor_loss	1.0000
19:760040:G:GA	donor_loss	1.0000
19:760040:G:GG	donor_gain	1.0000
19:760042:GA:G	donor_loss	1.0000
19:761619:CTGTA:C	acceptor_loss	1.0000
19:761620:T:A	acceptor_gain	1.0000
19:761620:TGTAG:T	acceptor_loss	1.0000
19:761621:GTAGT:G	acceptor_loss	1.0000
19:761622:TA:T	acceptor_loss	1.0000
19:761623:A:AG	acceptor_gain	1.0000
19:761623:AGTA:A	acceptor_loss	1.0000
19:761623:AGTAC:A	acceptor_gain	1.0000
19:761624:G:A	acceptor_loss	1.0000
19:761624:G:GA	acceptor_gain	1.0000
19:761624:GT:G	acceptor_gain	1.0000

AlphaMissense

4383 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:757544:T:C	F200L	0.990
19:757546:C:A	F200L	0.990
19:757546:C:G	F200L	0.990
19:757836:T:C	I297T	0.988
19:757824:T:A	I293N	0.986
19:757830:G:C	R295P	0.986
19:763554:G:C	W668C	0.986
19:763554:G:T	W668C	0.986
19:757523:T:C	F193L	0.983
19:757525:C:A	F193L	0.983
19:757525:C:G	F193L	0.983
19:757839:G:C	R298P	0.978
19:757854:G:C	R303P	0.978
19:757545:T:C	F200S	0.976
19:757836:T:G	I297S	0.976
19:757866:T:C	L307P	0.973
19:763552:T:A	W668R	0.971
19:763552:T:C	W668R	0.971
19:757545:T:G	F200C	0.969
19:757853:C:A	R303S	0.968
19:758616:G:C	R557P	0.968
19:757844:G:C	A300P	0.966
19:757524:T:G	F193C	0.963
19:757859:G:C	A305P	0.963
19:757869:G:C	R308P	0.963
19:757524:T:C	F193S	0.962
19:757824:T:G	I293S	0.959
19:757848:A:C	Q301P	0.959
19:758666:T:C	F574L	0.957
19:758668:C:A	F574L	0.957

dbSNP variants (sampled 300 via entrez): RS1000340403 (19:753463 G>C), RS1000370787 (19:749415 G>A,C,T), RS1000590603 (19:748674 T>C), RS1000676447 (19:752424 T>C), RS1000677074 (19:757463 C>A), RS1001049191 (19:760121 T>G), RS1001106110 (19:748544 T>TC), RS1001127328 (19:756805 A>T), RS1001178613 (19:762195 A>C,G,T), RS1001232310 (19:751267 G>A), RS1001233224 (19:749511 G>A), RS1001567732 (19:756423 T>A,G), RS1001735713 (19:753359 G>A), RS1001786524 (19:757251 C>G), RS1001874673 (19:764042 A>C,G,T)

Disease associations

OMIM: gene MIM:615289 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL4295893 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

PharmGKB variants

1 variants.

Variant	Genes	Level	Score	#Clin annots	Drugs
rs8110536	MISP		0.00	0

CTD chemical–gene interactions

34 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Benzo(a)pyrene	affects methylation, decreases expression, decreases methylation	3
perfluorooctanoic acid	increases expression	2
Tetrachlorodibenzodioxin	increases expression	2
Tobacco Smoke Pollution	affects expression, increases expression	2
aristolochic acid I	increases expression	1
tris(2-butoxyethyl) phosphate	affects expression	1
arsenite	decreases methylation	1
tris(1,3-dichloro-2-propyl)phosphate	increases expression	1
sodium arsenite	increases expression	1
coumarin	increases phosphorylation	1
perfluorooctane sulfonic acid	decreases expression	1
perfluoro-n-nonanoic acid	increases expression	1
perfluorohexanesulfonic acid	increases expression	1
abrine	increases expression	1
Resveratrol	affects cotreatment, decreases expression	1
Arsenic Trioxide	decreases response to substance	1
Air Pollutants	decreases expression, increases abundance	1
Arsenic	affects methylation	1
Caffeine	affects phosphorylation	1
Cisplatin	decreases expression	1
Estradiol	affects cotreatment, increases expression	1
Ivermectin	decreases expression	1
Plant Extracts	affects cotreatment, decreases expression	1
Quercetin	decreases phosphorylation	1
Selenium	increases expression	1
Smoke	increases expression	1
Urethane	decreases expression	1
Valproic Acid	increases methylation	1
Cyclosporine	increases expression	1
Cadmium Chloride	increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4118966	Binding	Binding affinity to MISP in human NCI-H358 cells at 1 uM by mass spectrometry based pull down assay	Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors. — Bioorg Med Chem

Cellosaurus cell lines

4 cell lines: 4 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B1X4	Abcam HeLa MISP KO	Cancer cell line	Female
CVCL_E2CF	HAP1 MISP (-) 2	Cancer cell line	Male
CVCL_E2CG	HAP1 MISP (-) 3	Cancer cell line	Male
CVCL_XQ56	HAP1 MISP (-) 1	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.