MLEC

Summary

MLEC (malectin, HGNC:28973) is a protein-coding gene on chromosome 12q24.31, encoding Malectin (Q14165). Carbohydrate-binding protein with a strong ligand preference for Glc2-N-glycan.

This gene encodes the carbohydrate-binding protein malectin which is a Type I membrane-anchored endoplasmic reticulum protein. This protein has an affinity for Glc2Man9GlcNAc2 (G2M9) N-glycans and is involved in regulating glycosylation in the endoplasmic reticulum. This protein has also been shown to interact with ribophorin I and may be involved in the directing the degradation of misfolded proteins. Alternate splicing results in multiple transcript variants.

Source: NCBI Gene 9761 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 30 total
• Druggable target: yes
• MANE Select transcript: NM_014730

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:28973
Approved symbol	MLEC
Name	malectin
Location	12q24.31
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000110917
Ensembl biotype	protein_coding
OMIM	613802
Entrez	9761

Gene structure

Transcript identifiers

Ensembl transcripts: 9 — 8 protein_coding, 1 protein_coding_CDS_not_defined

ENST00000228506, ENST00000412616, ENST00000535413, ENST00000535656, ENST00000545525, ENST00000873883, ENST00000873884, ENST00000873885, ENST00000915485

RefSeq mRNA: 3 — MANE Select: NM_014730 NM_001303627, NM_001303628, NM_014730

CCDS: CCDS76609, CCDS9206

Canonical transcript exons

ENST00000228506 — 5 exons

Exon	Start	End
ENSE00000756534	120696316	120701859
ENSE00001249670	120695095	120695152
ENSE00001249678	120694824	120695000
ENSE00001249688	120694091	120694269
ENSE00002306378	120687149	120687531

Expression profiles

Bgee: expression breadth ubiquitous, 303 present calls, max score 98.61.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 71.5404 / max 1451.4290, expressed in 1822 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
128376	71.4961	1822
128378	0.0443	8

Top tissues by expression

303 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
mucosa of sigmoid colon	UBERON:0004993	98.61	gold quality
corpus epididymis	UBERON:0004359	98.53	gold quality
pancreatic ductal cell	CL:0002079	98.34	gold quality
pylorus	UBERON:0001166	98.24	gold quality
colonic mucosa	UBERON:0000317	98.04	gold quality
rectum	UBERON:0001052	97.99	gold quality
adrenal tissue	UBERON:0018303	97.97	gold quality
ileal mucosa	UBERON:0000331	97.80	gold quality
right adrenal gland cortex	UBERON:0035827	97.77	gold quality
cauda epididymis	UBERON:0004360	97.69	gold quality
adrenal cortex	UBERON:0001235	97.68	gold quality
left adrenal gland	UBERON:0001234	97.67	gold quality
right adrenal gland	UBERON:0001233	97.62	gold quality
adrenal gland	UBERON:0002369	97.61	gold quality
left adrenal gland cortex	UBERON:0035825	97.59	gold quality
mucosa of transverse colon	UBERON:0004991	97.53	gold quality
stromal cell of endometrium	CL:0002255	97.39	gold quality
caput epididymis	UBERON:0004358	97.31	gold quality
cardia of stomach	UBERON:0001162	97.14	gold quality
medial globus pallidus	UBERON:0002477	96.98	gold quality
duodenum	UBERON:0002114	96.96	gold quality
body of pancreas	UBERON:0001150	96.87	gold quality
decidua	UBERON:0002450	96.76	gold quality
colonic epithelium	UBERON:0000397	96.66	gold quality
islet of Langerhans	UBERON:0000006	96.41	gold quality
seminal vesicle	UBERON:0000998	96.31	gold quality
pancreas	UBERON:0001264	96.28	gold quality
right testis	UBERON:0004534	96.21	gold quality
left testis	UBERON:0004533	96.20	gold quality
renal medulla	UBERON:0000362	96.12	gold quality

Single-cell (SCXA)

Detected in 11 experiment(s), a significant marker in 9.

Experiment	Marker?	Max mean expression
E-GEOD-125970	yes	470.13
E-MTAB-9467	yes	48.77
E-HCAD-1	yes	43.33
E-HCAD-6	yes	23.72
E-CURD-122	yes	22.22
E-MTAB-9067	yes	14.22
E-CURD-112	yes	5.80
E-GEOD-81608	yes	4.43
E-MTAB-4850	no	236.29
E-HCAD-13	no	2.99
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

227 targeting MLEC, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4533	100.00	69.48	2758
HSA-MIR-6758-5P	100.00	66.21	1470
HSA-MIR-6856-5P	100.00	65.47	1298
HSA-MIR-5193	100.00	67.26	1744
HSA-MIR-4262	100.00	73.26	3931
HSA-MIR-1252-5P	100.00	69.80	2774
HSA-MIR-4673	100.00	66.64	1490
HSA-MIR-8485	100.00	77.57	4731
HSA-MIR-3925-3P	100.00	69.95	1237
HSA-MIR-6867-5P	100.00	82.21	3464
HSA-MIR-3667-3P	99.99	67.17	1636
HSA-MIR-371B-5P	99.99	75.34	4759
HSA-MIR-520G-5P	99.99	66.76	658
HSA-MIR-3662	99.99	73.82	5684
HSA-MIR-150-5P	99.99	66.69	1976
HSA-MIR-181A-5P	99.99	72.96	2995
HSA-MIR-181B-5P	99.99	72.97	2996
HSA-MIR-181C-5P	99.99	72.95	2996
HSA-MIR-181D-5P	99.99	73.04	2997
HSA-MIR-373-5P	99.98	75.36	4753
HSA-MIR-616-5P	99.98	75.58	4775
HSA-MIR-4645-5P	99.98	65.81	1284
HSA-MIR-607	99.97	73.62	5593
HSA-MIR-5688	99.96	73.23	4504
HSA-MIR-495-3P	99.96	72.81	4197
HSA-MIR-548AA	99.96	70.64	3753
HSA-MIR-548AP-3P	99.96	70.64	3753
HSA-MIR-548T-3P	99.96	70.64	3753
HSA-MIR-551B-5P	99.96	71.28	3493
HSA-MIR-3605-5P	99.96	67.12	932

Literature-anchored findings (GeneRIF, showing 5)

• Malectin is a novel carbohydrate-binding protein of the endoplasmic reticulum and a candidate player in the early steps of protein N-glycosylation (PMID:18524852)
• These observations suggest a possible role of malectin in regulating newly synthesized glycoproteins via G2M9 recognition. (PMID:21813736)
• Data indicate that malectin functions by forming a complex with ribophorin I. (PMID:22988243)
• These results clearly demonstrate that the association of malectin with ribophorin I is required to capture misfolded alpha1-antitrypsin and direct them to the degradation pathway. (PMID:25451265)
• The results of in vitro studies suggest that C alleles of rs10431386 and rs7964786 on MLEC promotes cerebral palsy (CP) by inhibiting M1 to M2 macrophage polarization. Generally, this work suggested the contribution of MLEC gene polymorphisms to the pathogenesis of CP (PMID:28972276)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	mlec	ENSDARG00000059630
mus_musculus	Mlec	ENSMUSG00000048578
rattus_norvegicus	Mlec	ENSRNOG00000064601
drosophila_melanogaster	CG9257	FBGN0032916
caenorhabditis_elegans		WBGENE00018423

Protein

Protein identifiers

Malectin — Q14165 (reviewed: Q14165)

All UniProt accessions (4): Q14165, F5GX14, F5H1S8, H0YG07

UniProt curated annotations — full annotation on UniProt →

Function. Carbohydrate-binding protein with a strong ligand preference for Glc2-N-glycan. May play a role in the early steps of protein N-glycosylation.

Subunit / interactions. Interacts with the oligosaccharyltransferase (OST) complex.

Subcellular location. Endoplasmic reticulum membrane.

Similarity. Belongs to the malectin family.

RefSeq proteins (3): NP_001290556, NP_001290557, NP_055545* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR021720	Malectin_dom	Domain
IPR039155	MLEC	Family

Pfam: PF11721

UniProt features (17 total): binding site 5, turn 2, topological domain 2, compositionally biased region 2, signal peptide 1, chain 1, glycosylation site 1, helix 1, transmembrane region 1, region of interest 1

Structure

Experimental structures (PDB)

5 structures.

PDB	Method	Resolution (Å)
9IL3	X-RAY DIFFRACTION	1.45
9ILA	X-RAY DIFFRACTION	1.56
9ILF	X-RAY DIFFRACTION	1.56
9IKP	X-RAY DIFFRACTION	1.68
6S7T	ELECTRON MICROSCOPY	3.5

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q14165-F1	81.03	0.63

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (5): 131; 132; 201; 82; 104

Glycosylation sites (1): 268

Function

Pathways and Gene Ontology

Reactome pathways

7 pathways

ID	Pathway
R-HSA-532668	N-glycan trimming in the ER and Calnexin/Calreticulin cycle
R-HSA-6798695	Neutrophil degranulation
R-HSA-168249	Innate Immune System
R-HSA-168256	Immune System
R-HSA-392499	Metabolism of proteins
R-HSA-446203	Asparagine N-linked glycosylation
R-HSA-597592	Post-translational protein modification

MSigDB gene sets: 361 (showing top): REACTOME_INNATE_IMMUNE_SYSTEM, KAAB_FAILED_HEART_ATRIUM_DN, GOCC_SECRETORY_GRANULE, GTTAAAG_MIR302B, MORF_HDAC2, PRAMOONJAGO_SOX4_TARGETS_DN, MONNIER_POSTRADIATION_TUMOR_ESCAPE_UP, COUP_01, CTCTAGA_MIR526C_MIR518F_MIR526A, GTGCCTT_MIR506, WEI_MYCN_TARGETS_WITH_E_BOX, GATA3_01, HNF4_DR1_Q3, MORF_RFC4, MORF_PRKDC

GO Biological Process (0):

GO Molecular Function (4): enzyme binding (GO:0019899), carbohydrate binding (GO:0030246), protein-folding chaperone binding (GO:0051087), protein binding (GO:0005515)

GO Cellular Component (6): endoplasmic reticulum (GO:0005783), endoplasmic reticulum membrane (GO:0005789), plasma membrane (GO:0005886), oligosaccharyltransferase complex (GO:0008250), membrane (GO:0016020), specific granule membrane (GO:0035579)

Reactome top-level categories

Rollup of top-5 pathways:

Category	Pathways
Asparagine N-linked glycosylation	1
Innate Immune System	1
Immune System	1
Post-translational protein modification	1
Metabolism of proteins	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
protein binding	2
binding	2
cytoplasm	1
endomembrane system	1
intracellular membrane-bounded organelle	1
organelle membrane	1
nuclear outer membrane-endoplasmic reticulum membrane network	1
endoplasmic reticulum subcompartment	1
membrane	1
cell periphery	1
endoplasmic reticulum membrane	1
membrane protein complex	1
endoplasmic reticulum protein-containing complex	1
transferase complex	1
cellular anatomical structure	1
secretory granule membrane	1
specific granule	1

Protein interactions and networks

STRING

672 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
MLEC	RPN1	P04843	801
MLEC	ERLEC1	Q96DZ1	727
MLEC	LMAN2	Q12907	628
MLEC	UGGT1	Q9NYU2	618
MLEC	EDEM2	Q9BV94	570
MLEC	CANX	P27824	547
MLEC	MOGS	Q13724	543
MLEC	CALR	P27797	522
MLEC	EDEM1	Q92611	519
MLEC	STT3A	P46977	513
MLEC	EDEM3	Q9BZQ6	503
MLEC	RPN2	P04844	492
MLEC	STT3B	Q8TCJ2	477
MLEC	OS9	Q13438	475
MLEC	UGGT2	Q9NYU1	474

IntAct

109 interactions, top by confidence:

A	B	Type	Score
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710
RPN1	MLEC	psi-mi:“MI:0915”(physical association)	0.690
MLEC	RPN1	psi-mi:“MI:0915”(physical association)	0.690
STT3A	RPN1	psi-mi:“MI:0914”(association)	0.560
RPN2	SMPD2	psi-mi:“MI:0914”(association)	0.530
DAD1	RPN1	psi-mi:“MI:0915”(physical association)	0.530
CEP78	CEP43	psi-mi:“MI:0914”(association)	0.530
RPN1	APBB1	psi-mi:“MI:0914”(association)	0.530
MLEC	UMOD	psi-mi:“MI:0407”(direct interaction)	0.440
	FER1L5	psi-mi:“MI:0915”(physical association)	0.400
Dctn3		psi-mi:“MI:0914”(association)	0.350
Nedd1		psi-mi:“MI:0914”(association)	0.350
TSNAX		psi-mi:“MI:0914”(association)	0.350
RAB7A		psi-mi:“MI:0914”(association)	0.350
Tmed2		psi-mi:“MI:0914”(association)	0.350
Trip11	PPL	psi-mi:“MI:0914”(association)	0.350
GOLT1B		psi-mi:“MI:0914”(association)	0.350
	ESYT2	psi-mi:“MI:0914”(association)	0.350
E5	ESYT2	psi-mi:“MI:0914”(association)	0.350
HSCB	RBP5	psi-mi:“MI:0914”(association)	0.350
DLST		psi-mi:“MI:0914”(association)	0.350

BioGRID (159): CTSD (Co-fractionation), RBM8A (Co-fractionation), MLEC (Affinity Capture-MS), MLEC (Proximity Label-MS), MLEC (Proximity Label-MS), MLEC (Proximity Label-MS), MLEC (Proximity Label-MS), MLEC (Proximity Label-MS), MLEC (Affinity Capture-MS), MLEC (Affinity Capture-MS), MLEC (Affinity Capture-MS), MLEC (Affinity Capture-MS), MLEC (Affinity Capture-MS), MLEC (Affinity Capture-MS), MLEC (Affinity Capture-MS)

ESM2 similar proteins: A0A7C9FSB8, A2TLM1, A6H7H7, B8BKI7, B9N1F9, B9SQI7, D2XV59, E0CSI1, F1N9S8, O00178, O08582, O35586, O35760, O48964, O48965, O76031, O81770, P11029, P11497, P58044, P69341, Q0J035, Q13085, Q13907, Q14165, Q1LZ95, Q1LZ96, Q28559, Q2R483, Q38929, Q39471, Q39472, Q39664, Q3UMR5, Q42553, Q4R4W5, Q5NVE1, Q5R8R6, Q5SWU9, Q5U2U0

Diamond homologs: A9C3P0, Q14165, Q5FVQ4, Q6INX3, Q6ZQI3, Q8AVF4

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
MLEC	“form complex”	“OST-B complex”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 115 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
PD-L1(CD274) glycosylation and translocation to plasma membrane	10	68.3×	3e-14
Maturation of spike protein	11	38.4×	4e-13
Regulation of CDH1 posttranslational processing and trafficking to plasma membrane	8	35.4×	5e-09
Maturation of DENV proteins	12	33.4×	2e-13
Translation of Structural Proteins	5	26.8×	8e-05
Late SARS-CoV-2 Infection Events	5	19.3×	3e-04
Degradation of CDH1	5	12.9×	2e-03
Anchoring of the basal body to the plasma membrane	7	10.4×	3e-04

GO biological processes:

GO term	Partners	Fold	FDR
obsolete protein N-linked glycosylation via asparagine	10	69.5×	6e-14
protein N-linked glycosylation	11	29.9×	3e-11
ERAD pathway	7	13.1×	3e-04
transmembrane transport	6	10.4×	5e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

30 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	17
Likely benign	0
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

739 predictions. Top by Δscore:

Variant	Effect	Δscore
12:120694082:T:A	acceptor_gain	1.0000
12:120694089:A:AG	acceptor_gain	1.0000
12:120694090:G:GT	acceptor_gain	1.0000
12:120694090:GC:G	acceptor_gain	1.0000
12:120694090:GCC:G	acceptor_gain	1.0000
12:120694090:GCCT:G	acceptor_gain	1.0000
12:120694090:GCCTC:G	acceptor_gain	1.0000
12:120694207:G:GT	donor_gain	1.0000
12:120694211:GGGAC:G	donor_gain	1.0000
12:120694268:AGG:A	donor_loss	1.0000
12:120694270:G:GA	donor_loss	1.0000
12:120694814:A:AG	acceptor_gain	1.0000
12:120694814:ATCCT:A	acceptor_gain	1.0000
12:120694815:T:G	acceptor_gain	1.0000
12:120694818:T:A	acceptor_gain	1.0000
12:120694819:G:A	acceptor_gain	1.0000
12:120694822:A:AG	acceptor_gain	1.0000
12:120694822:AG:A	acceptor_gain	1.0000
12:120694823:G:A	acceptor_gain	1.0000
12:120694823:G:GG	acceptor_gain	1.0000
12:120694823:GGT:G	acceptor_gain	1.0000
12:120694823:GGTA:G	acceptor_gain	1.0000
12:120694823:GGTAT:G	acceptor_gain	1.0000
12:120694999:AGG:A	donor_loss	1.0000
12:120695000:GGTA:G	donor_loss	1.0000
12:120695002:T:G	donor_loss	1.0000
12:120695089:TCCTA:T	acceptor_loss	1.0000
12:120695090:CCTAG:C	acceptor_loss	1.0000
12:120695091:CTA:C	acceptor_loss	1.0000
12:120695092:TA:T	acceptor_loss	1.0000

AlphaMissense

1891 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
12:120687460:G:A	G55D	1.000
12:120687525:G:C	G77R	1.000
12:120694093:T:C	S80P	1.000
12:120694102:G:C	G83R	1.000
12:120694103:G:A	G83D	1.000
12:120694103:G:T	G83V	1.000
12:120694163:G:C	R103P	1.000
12:120694237:G:C	A128P	1.000
12:120694240:G:A	E129K	1.000
12:120694241:A:T	E129V	1.000
12:120694242:G:C	E129D	1.000
12:120694242:G:T	E129D	1.000
12:120694246:T:G	Y131D	1.000
12:120694249:T:A	F132I	1.000
12:120694249:T:C	F132L	1.000
12:120694249:T:G	F132V	1.000
12:120694250:T:C	F132S	1.000
12:120694250:T:G	F132C	1.000
12:120694251:T:A	F132L	1.000
12:120694251:T:G	F132L	1.000
12:120694267:A:G	K138E	1.000
12:120694269:G:C	K138N	1.000
12:120694269:G:T	K138N	1.000
12:120694827:T:C	F140L	1.000
12:120694828:T:C	F140S	1.000
12:120694829:T:A	F140L	1.000
12:120694829:T:G	F140L	1.000
12:120694869:G:C	D154H	1.000
12:120694870:A:T	D154V	1.000
12:120694875:T:C	F156L	1.000

dbSNP variants (sampled 300 via entrez): RS1000066995 (12:120691916 T>C), RS1000293643 (12:120691532 T>C,G), RS1000317612 (12:120699049 G>C), RS1000586845 (12:120696798 C>T), RS1000663938 (12:120691287 C>T), RS1000737325 (12:120688862 C>T), RS1001089126 (12:120686774 T>C,G), RS1001120538 (12:120686923 C>T), RS1001193095 (12:120692765 G>A,T), RS1001422854 (12:120687764 CTCA>C), RS1002226760 (12:120694749 G>A,T), RS1002602082 (12:120685542 G>A), RS1002941647 (12:120691474 A>G,T), RS1003073200 (12:120698615 G>C,T), RS1003096789 (12:120689269 T>C,G)

Disease associations

OMIM: gene MIM:613802 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST005666_4	Fractional excretion of metabolites in chronic kidney disease	3.000000e-14
GCST006249_30	Serum metabolite levels	4.000000e-177
GCST012020_371	Serum metabolite levels	3.000000e-161
GCST012020_372	Serum metabolite levels	1.000000e-31

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0005116	urinary metabolite measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067153 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 4 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.15	Kd	7055	nM	CHEMBL5653589
5.15	ED50	7055	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 4 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148754: Binding affinity to human MLEC incubated for 45 mins by Kinobead based pull down assay	kd	7.0548	uM

CTD chemical–gene interactions

42 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	affects expression, increases expression	3
bisphenol A	increases expression	2
Acetaminophen	decreases expression, increases expression	2
Tunicamycin	increases expression	2
bisphenol F	affects cotreatment, increases expression	1
testosterone enanthate	affects expression	1
triphenyl phosphate	affects expression	1
sodium arsenate	decreases expression	1
tris(1,3-dichloro-2-propyl)phosphate	decreases expression	1
di-n-butylphosphoric acid	affects expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	increases expression	1
nutlin 3	affects cotreatment, increases secretion	1
abrine	decreases expression	1
bisphenol AF	increases expression	1
Leflunomide	decreases expression	1
Air Pollutants	decreases expression	1
Arsenic	affects methylation	1
Cisplatin	increases expression	1
Dactinomycin	affects cotreatment, increases secretion	1
Dexamethasone	affects cotreatment, increases expression	1
Diazinon	increases methylation	1
Diuron	decreases expression	1
Indomethacin	affects cotreatment, increases expression	1
Ivermectin	decreases expression	1
Methyl Methanesulfonate	decreases expression	1
Ribonucleotides	affects binding	1
Rotenone	increases expression	1
Selenium	affects cotreatment, decreases expression, increases expression	1
Dihydrotestosterone	increases expression	1
Dronabinol	increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5651796	Binding	Binding affinity to human MLEC incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

1 cell lines: 1 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B1X7	Abcam HeLa MLEC KO	Cancer cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.