MOB2

Summary

MOB2 (MOB kinase activator 2, HGNC:24904) is a protein-coding gene on chromosome 11p15.5, encoding MOB kinase activator 2 (Q70IA6). Stimulates the autophosphorylation and kinase activity of STK38 and STK38L.

Predicted to enable protein kinase activator activity. Predicted to be involved in signal transduction. Predicted to act upstream of or within actin cytoskeleton organization; positive regulation of neuron projection development; and positive regulation of protein phosphorylation. Located in cytosol; nucleolus; and nucleoplasm.

Source: NCBI Gene 81532 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 62 total
• MANE Select transcript: NM_001172223

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:24904
Approved symbol	MOB2
Name	MOB kinase activator 2
Location	11p15.5
Locus type	gene with protein product
Status	Approved
Aliases	HCCA2
Ensembl gene	ENSG00000182208
Ensembl biotype	protein_coding
OMIM	611969
Entrez	81532

Gene structure

Transcript identifiers

Ensembl transcripts: 6 — 4 retained_intron, 1 protein_coding, 1 protein_coding_CDS_not_defined

ENST00000329957, ENST00000526462, ENST00000526698, ENST00000529380, ENST00000529897, ENST00000531976

RefSeq mRNA: 2 — MANE Select: NM_001172223 NM_001172223, NM_053005

CCDS: CCDS53591

Canonical transcript exons

ENST00000329957 — 5 exons

Exon	Start	End
ENSE00002165278	1469457	1470488
ENSE00002192006	1486447	1486779
ENSE00003509391	1471295	1471419
ENSE00003592684	1480393	1480486
ENSE00003659103	1480725	1480885

Expression profiles

Bgee: expression breadth ubiquitous, 248 present calls, max score 97.62.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.1523 / max 18.1431, expressed in 61 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
117970	0.0878	38
117971	0.0645	38

Top tissues by expression

253 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
popliteal artery	UBERON:0002250	97.62	gold quality
tibial artery	UBERON:0007610	97.62	gold quality
right coronary artery	UBERON:0001625	97.30	gold quality
muscle layer of sigmoid colon	UBERON:0035805	97.06	gold quality
lower esophagus muscularis layer	UBERON:0035833	96.98	gold quality
lower esophagus	UBERON:0013473	96.97	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	96.81	gold quality
left coronary artery	UBERON:0001626	96.57	gold quality
aorta	UBERON:0000947	96.56	gold quality
coronary artery	UBERON:0001621	96.50	gold quality
skin of leg	UBERON:0001511	96.48	gold quality
mucosa of stomach	UBERON:0001199	95.99	gold quality
skin of abdomen	UBERON:0001416	95.98	gold quality
smooth muscle tissue	UBERON:0001135	95.34	gold quality
thoracic aorta	UBERON:0001515	95.27	gold quality
ascending aorta	UBERON:0001496	95.20	gold quality
descending thoracic aorta	UBERON:0002345	95.02	gold quality
zone of skin	UBERON:0000014	94.99	gold quality
left uterine tube	UBERON:0001303	94.97	gold quality
ectocervix	UBERON:0012249	94.87	gold quality
gastrocnemius	UBERON:0001388	94.80	gold quality
esophagus	UBERON:0001043	94.76	gold quality
apex of heart	UBERON:0002098	94.70	gold quality
right lung	UBERON:0002167	94.65	gold quality
granulocyte	CL:0000094	94.62	gold quality
lower esophagus mucosa	UBERON:0035834	94.59	gold quality
muscle of leg	UBERON:0001383	94.49	gold quality
body of uterus	UBERON:0009853	94.31	gold quality
hindlimb stylopod muscle	UBERON:0004252	94.17	gold quality
endocervix	UBERON:0000458	93.94	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	5.34
E-MTAB-7249	no	31.44

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

23 targeting MOB2, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-765	99.84	68.24	2442
HSA-MIR-11181-3P	99.75	66.38	2205
HSA-MIR-1827	99.63	68.57	3265
HSA-MIR-6836-5P	99.60	65.62	1538
HSA-MIR-6132	99.60	65.83	1554
HSA-MIR-3123	99.47	67.15	2693
HSA-MIR-5683	99.36	68.59	2083
HSA-MIR-760	98.81	66.65	1392
HSA-MIR-519A-2-5P	98.78	71.74	1401
HSA-MIR-520B-5P	98.78	71.74	1401
HSA-MIR-6761-5P	98.71	68.03	1504
HSA-MIR-3944-5P	98.50	67.55	997
HSA-MIR-4722-5P	98.46	66.34	1611
HSA-MIR-7158-3P	98.46	66.45	728
HSA-MIR-4684-5P	98.29	67.99	1650
HSA-MIR-6771-3P	98.20	66.53	971
HSA-MIR-93-3P	98.15	66.65	1309
HSA-MIR-1245B-3P	98.01	68.91	1387
HSA-MIR-3661	97.83	67.30	705
HSA-MIR-631	97.05	66.93	602
HSA-MIR-3189-3P	96.80	66.34	896
HSA-MIR-6782-5P	96.45	64.42	612
HSA-MIR-3130-3P	94.98	66.97	574

Literature-anchored findings (GeneRIF, showing 6)

• Binds to and stimulates the kinase activity of the related human serine-threonine kinases NDR1 and NDR2. (PMID:15067004)
• Data indicate that human MOB2 is a negative regulator of human NDR kinases in biochemical and biological settings. (PMID:20624913)
• MOB2 overexpression induces apoptosis and inhibits the growth of human hepatic cancer cells (PMID:23326134)
• hMOB2 interacts with RAD50, facilitating the recruitment of the MRE11-RAD50-NBS1 (MRN) DNA damage sensor complex and activated ATM to DNA damaged chromatin. (PMID:25460043)
• The altered cell-matrix adhesion and cell motility induced by hMOB2 expression was caused by the assembly of focal adhesions. (PMID:25779224)
• MOB2 suppresses GBM cell migration and invasion via regulation of FAK/Akt and cAMP/PKA signaling. (PMID:32286266)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	mob2b	ENSDARG00000012005
danio_rerio	mob2a	ENSDARG00000023214
mus_musculus	Mob2	ENSMUSG00000025147
rattus_norvegicus	Mob2	ENSRNOG00000020044
caenorhabditis_elegans		WBGENE00008601

Paralogs (6): MOB1A (ENSG00000114978), MOB4 (ENSG00000115540), MOB3B (ENSG00000120162), MOB3C (ENSG00000142961), MOB3A (ENSG00000172081), MOB1B (ENSG00000173542)

Protein

Protein identifiers

MOB kinase activator 2 — Q70IA6 (reviewed: Q70IA6)

Alternative names: HCCA2, Mob2 homolog, Mps one binder kinase activator-like 2

All UniProt accessions (1): Q70IA6

UniProt curated annotations — full annotation on UniProt →

Function. Stimulates the autophosphorylation and kinase activity of STK38 and STK38L.

Subunit / interactions. Binds STK38 and STK38L.

Subcellular location. Nucleus. Cytoplasm. Perinuclear region.

Post-translational modifications. Phosphorylated.

Similarity. Belongs to the MOB1/phocein family.

Isoforms (3)

UniProt ID	Names	Canonical?
Q70IA6-1	1	yes
Q70IA6-2	2
Q70IA6-3	3

RefSeq proteins (2): NP_001165694, NP_443731 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR005301	MOB_kinase_act_fam	Family
IPR036703	MOB_kinase_act_sf	Homologous_superfamily

Pfam: PF03637

UniProt features (13 total): binding site 4, splice variant 3, region of interest 2, sequence conflict 2, chain 1, compositionally biased region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q70IA6-F1	86.60	0.74

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (4): 78; 83; 157; 162

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 69 (showing top): GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_UP, IVANOVA_HEMATOPOIESIS_MATURE_CELL, GOMF_KINASE_ACTIVATOR_ACTIVITY, GOCC_NUCLEOLUS, LINDGREN_BLADDER_CANCER_CLUSTER_1_DN, BOCHKIS_FOXA2_TARGETS, GOMF_ENZYME_ACTIVATOR_ACTIVITY, GOMF_ENZYME_REGULATOR_ACTIVITY, GARGALOVIC_RESPONSE_TO_OXIDIZED_PHOSPHOLIPIDS_BLACK_UP, GSE10240_CTRL_VS_IL17_STIM_PRIMARY_BRONCHIAL_EPITHELIAL_CELLS_DN, ID2_TARGET_GENES, NFKBIA_TARGET_GENES, MIR5683, MIR1245B_3P, GSE11924_TH2_VS_TH17_CD4_TCELL_DN

GO Biological Process (1): signal transduction (GO:0007165)

GO Molecular Function (3): protein kinase activator activity (GO:0030295), metal ion binding (GO:0046872), protein binding (GO:0005515)

GO Cellular Component (6): nucleus (GO:0005634), nucleoplasm (GO:0005654), nucleolus (GO:0005730), cytoplasm (GO:0005737), cytosol (GO:0005829), perinuclear region of cytoplasm (GO:0048471)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	4
nuclear lumen	2
cytoplasm	2
cell communication	1
cellular process	1
signaling	1
regulation of cellular process	1
cellular response to stimulus	1
protein kinase activity	1
kinase activator activity	1
protein kinase regulator activity	1
cation binding	1
binding	1
intracellular membrane-bounded organelle	1
intracellular membraneless organelle	1
intracellular anatomical structure	1

Protein interactions and networks

STRING

1620 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
MOB2	STK38	Q15208	845
MOB2	STK38L	Q9Y2H1	791
MOB2	MOB4	Q9Y3A3	781
MOB2	NTSR1	P30989	506
MOB2	LATS1	O95835	485
MOB2	KRTAP5-1	Q6L8H4	477
MOB2	FRYL	O94915	474
MOB2	TSEN15	Q8WW01	454
MOB2	DIMT1	Q9UNQ2	446
MOB2	SAV1	Q9H4B6	433
MOB2	LATS2	Q9NRM7	419
MOB2	STK24	Q9Y6E0	394
MOB2	DCHS1	Q96JQ0	390
MOB2	SGIP1	Q9BQI5	378
MOB2	HSPBP1	Q9NZL4	377

IntAct

73 interactions, top by confidence:

A	B	Type	Score
STK38L	MOB2	psi-mi:“MI:0914”(association)	0.910
MOB2	STK38L	psi-mi:“MI:0915”(physical association)	0.910
MED4	MED19	psi-mi:“MI:0914”(association)	0.900
STK38	MOB2	psi-mi:“MI:0914”(association)	0.710
MOB2	STK38	psi-mi:“MI:0915”(physical association)	0.710
YWHAE	PIK3C2A	psi-mi:“MI:0914”(association)	0.570
MOB2	OR5H1	psi-mi:“MI:0915”(physical association)	0.560
MOB2	RETREG3	psi-mi:“MI:0915”(physical association)	0.560
MOB2	FAAP20	psi-mi:“MI:0915”(physical association)	0.560
MOB2	SFT2D2	psi-mi:“MI:0915”(physical association)	0.560
MOB2	MIIP	psi-mi:“MI:0915”(physical association)	0.560
MOB2	CCDC90B	psi-mi:“MI:0915”(physical association)	0.560
MOB2	FRA10AC1	psi-mi:“MI:0915”(physical association)	0.560
MOB2	ATG3	psi-mi:“MI:0915”(physical association)	0.560
MOB2	NTAQ1	psi-mi:“MI:0915”(physical association)	0.560
MIIP	MOB2	psi-mi:“MI:0915”(physical association)	0.560
OR5H1	MOB2	psi-mi:“MI:0915”(physical association)	0.560
FAAP20	MOB2	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (148): MOB2 (Affinity Capture-MS), MOB2 (Affinity Capture-MS), MOB2 (Affinity Capture-MS), MOB2 (Affinity Capture-MS), MOB2 (Proximity Label-MS), MOB2 (Affinity Capture-MS), MOB2 (Affinity Capture-MS), MOB2 (Affinity Capture-MS), MOB2 (Affinity Capture-MS), MOB2 (Affinity Capture-MS), MOB2 (Affinity Capture-MS), MOB2 (Affinity Capture-MS), MOB2 (Two-hybrid), MOB2 (Two-hybrid), MOB2 (Two-hybrid)

ESM2 similar proteins: A0A223HDI2, A2BG43, B0BLT4, B0BNM9, B0YN54, O22797, O64587, O94360, P40124, P68265, P68266, Q01518, Q08163, Q0VCQ0, Q2WBN3, Q3SYV4, Q3T1J9, Q4R4I6, Q54XJ0, Q5F495, Q5HZ92, Q5RAE0, Q5RDB1, Q5TA50, Q5XIS2, Q63ZQ3, Q66JG2, Q6DBQ8, Q6NLQ3, Q6NU44, Q6PEB6, Q6Z6S1, Q70IA6, Q7L9L4, Q8BPB0, Q8BS40, Q8GYX0, Q8VI63, Q921Y0, Q949G5

Diamond homologs: F4K494, F4K495, O74558, O94360, P40484, P43563, Q29RK9, Q2LZ59, Q2WBN3, Q3T1J9, Q54BM4, Q54CR8, Q54QV0, Q54XJ0, Q58D63, Q5ABC6, Q5EAA4, Q5R5Z0, Q5RAE0, Q70IA6, Q70IA8, Q7L9L4, Q86TA1, Q8BJG4, Q8BPB0, Q8BSU7, Q8GYX0, Q8IQG1, Q8VE04, Q8VI63, Q921Y0, Q949G5, Q95RA8, Q96BX8, Q9FHI1, Q9H8S9, Q9P601, Q9VL13

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

62 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	32
Likely benign	22
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1018 predictions. Top by Δscore:

Variant	Effect	Δscore
11:1471315:T:TA	donor_gain	1.0000
11:1471416:CTGT:C	acceptor_gain	1.0000
11:1471417:TGT:T	acceptor_gain	1.0000
11:1471418:GT:G	acceptor_gain	1.0000
11:1471420:C:CC	acceptor_gain	1.0000
11:1471421:T:G	acceptor_loss	1.0000
11:1480385:GCACT:G	donor_loss	1.0000
11:1480386:CACT:C	donor_loss	1.0000
11:1480387:ACTCA:A	donor_loss	1.0000
11:1480388:C:G	donor_loss	1.0000
11:1480389:TCA:T	donor_loss	1.0000
11:1480390:CACG:C	donor_loss	1.0000
11:1480391:A:AC	donor_gain	1.0000
11:1480391:A:AG	donor_loss	1.0000
11:1480392:C:A	donor_loss	1.0000
11:1480392:C:CT	donor_gain	1.0000
11:1480392:CGT:C	donor_gain	1.0000
11:1480392:CGTG:C	donor_gain	1.0000
11:1480482:CGTGG:C	acceptor_gain	1.0000
11:1480487:C:CC	acceptor_gain	1.0000
11:1480723:A:AC	donor_gain	1.0000
11:1480724:C:CC	donor_gain	1.0000
11:1480778:T:TA	donor_gain	1.0000
11:1470484:TCTGC:T	acceptor_loss	0.9900
11:1470487:GCC:G	acceptor_loss	0.9900
11:1470488:CCTG:C	acceptor_loss	0.9900
11:1470489:C:CC	acceptor_gain	0.9900
11:1471422:G:C	acceptor_gain	0.9900
11:1471422:G:GC	acceptor_gain	0.9900
11:1472771:T:TA	donor_gain	0.9900

AlphaMissense

1779 scored. Top likely-pathogenic:

dbSNP variants (sampled 300 via entrez): RS1000034567 (11:1532174 C>T), RS1000151139 (11:1498200 G>A), RS1000265720 (11:1526854 G>A,C), RS1000274828 (11:1509439 A>G), RS1000292172 (11:1492766 C>A), RS1000338520 (11:1534080 A>G), RS1000380954 (11:1502863 T>C), RS1000403535 (11:1484117 A>G), RS1000410060 (11:1501010 A>G), RS1000415143 (11:1492557 G>A), RS1000447345 (11:1470606 C>A,T), RS1000487310 (11:1485687 T>G), RS1000503294 (11:1523144 T>C), RS1000505270 (11:1519810 G>A), RS1000513204 (11:1489107 A>G)

Disease associations

OMIM: gene MIM:611969 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST006085_45	Prostate cancer	7.000000e-09
GCST010002_230	Refractive error	4.000000e-13
GCST010703_211	Brain morphology (MOSTest)	2.000000e-11
GCST011364_17	Myocardial infarction	3.000000e-08

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0004346	neuroimaging measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

28 total (human), top 28 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Arsenic	affects methylation, affects cotreatment, increases abundance, increases expression	2
aristolochic acid I	increases expression	1
GSK-J4	increases expression	1
dicrotophos	decreases expression	1
triphenyl phosphate	affects expression	1
bisphenol A	affects methylation, affects cotreatment, increases methylation	1
sodium arsenite	affects cotreatment, increases abundance, increases expression	1
manganese chloride	increases abundance, increases expression, affects cotreatment	1
benzo(e)pyrene	decreases methylation	1
aflatoxin B2	decreases methylation	1
abrine	increases expression	1
bisphenol S	affects methylation, affects cotreatment, increases methylation	1
PCI 5002	affects cotreatment, increases expression	1
Fulvestrant	increases methylation, affects methylation, affects cotreatment	1
Leflunomide	decreases expression	1
Aspirin	increases expression	1
Benzo(a)pyrene	affects methylation	1
Estradiol	decreases expression	1
Manganese	increases expression, affects cotreatment, increases abundance	1
Methapyrilene	decreases methylation	1
Smoke	decreases expression	1
Tobacco Smoke Pollution	increases expression	1
Urethane	increases expression	1
Zinc	affects cotreatment, increases expression	1
Cyclosporine	increases expression	1
Aflatoxin B1	increases methylation	1
Antirheumatic Agents	increases expression	1
Particulate Matter	increases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.