MRGPRF

Summary

MRGPRF (MAS related GPR family member F, HGNC:24828) is a protein-coding gene on chromosome 11q13.3, encoding Mas-related G-protein coupled receptor member F (Q96AM1). Orphan receptor.

Predicted to enable G protein-coupled receptor activity. Predicted to be involved in G protein-coupled receptor signaling pathway. Located in nuclear membrane; nucleoplasm; and plasma membrane.

Source: NCBI Gene 116535 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 75 total
• Druggable target: yes
• MANE Select transcript: NM_145015

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:24828
Approved symbol	MRGPRF
Name	MAS related GPR family member F
Location	11q13.3
Locus type	gene with protein product
Status	Approved
Aliases	MGC21621, mrgF
Ensembl gene	ENSG00000172935
Ensembl biotype	protein_coding
OMIM	607233
Entrez	116535

Gene structure

Transcript identifiers

Ensembl transcripts: 10 — 10 protein_coding

ENST00000309099, ENST00000320913, ENST00000885480, ENST00000885481, ENST00000885482, ENST00000885483, ENST00000885484, ENST00000885485, ENST00000925381, ENST00000964385

RefSeq mRNA: 2 — MANE Select: NM_145015 NM_001098515, NM_145015

CCDS: CCDS8188

Canonical transcript exons

ENST00000309099 — 3 exons

Exon	Start	End
ENSE00001241938	69004398	69006261
ENSE00001387845	69009854	69009957
ENSE00002243872	69013083	69013246

Expression profiles

Bgee: expression breadth ubiquitous, 214 present calls, max score 98.64.

FANTOM5 (CAGE): breadth broad, TPM avg 8.0469 / max 204.5209, expressed in 873 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
121023	6.0081	840
121022	1.7531	552
121024	0.2857	179

Top tissues by expression

247 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
mucosa of stomach	UBERON:0001199	98.64	gold quality
left uterine tube	UBERON:0001303	98.14	gold quality
saphenous vein	UBERON:0007318	97.77	gold quality
body of uterus	UBERON:0009853	97.73	gold quality
urethra	UBERON:0000057	97.58	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	97.27	gold quality
myometrium	UBERON:0001296	97.10	gold quality
muscle layer of sigmoid colon	UBERON:0035805	96.99	gold quality
cauda epididymis	UBERON:0004360	96.96	gold quality
lower esophagus muscularis layer	UBERON:0035833	96.89	gold quality
endocervix	UBERON:0000458	96.87	gold quality
lower esophagus	UBERON:0013473	96.82	gold quality
nipple	UBERON:0002030	96.52	gold quality
popliteal artery	UBERON:0002250	95.98	gold quality
tibial artery	UBERON:0007610	95.97	gold quality
aorta	UBERON:0000947	95.89	gold quality
right coronary artery	UBERON:0001625	95.86	gold quality
thoracic aorta	UBERON:0001515	95.82	gold quality
ascending aorta	UBERON:0001496	95.72	gold quality
descending thoracic aorta	UBERON:0002345	95.48	gold quality
pericardium	UBERON:0002407	94.98	gold quality
smooth muscle tissue	UBERON:0001135	94.74	gold quality
ectocervix	UBERON:0012249	94.53	gold quality
vena cava	UBERON:0004087	94.48	gold quality
left coronary artery	UBERON:0001626	93.94	gold quality
coronary artery	UBERON:0001621	93.78	gold quality
seminal vesicle	UBERON:0000998	93.07	gold quality
uterine cervix	UBERON:0000002	92.78	gold quality
penis	UBERON:0000989	91.99	gold quality
right ovary	UBERON:0002118	91.96	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 3.

Experiment	Marker?	Max mean expression
E-HCAD-10	yes	19.04
E-ANND-3	yes	9.03
E-HCAD-11	yes	7.89

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): HBP1, HIF1A, HOXB2, JUN, SP1, TBX10, ZGPAT

miRNA regulators (miRDB)

37 targeting MRGPRF, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-5582-3P	99.86	72.48	4221
HSA-MIR-4447	99.85	67.81	2900
HSA-MIR-6875-3P	99.82	70.26	2983
HSA-MIR-6756-5P	99.82	67.97	2466
HSA-MIR-4659A-3P	99.80	72.62	4248
HSA-MIR-4659B-3P	99.80	72.62	4248
HSA-MIR-6764-5P	99.75	67.89	2304
HSA-MIR-33A-3P	99.70	70.27	3362
HSA-MIR-6766-5P	99.68	67.70	2325
HSA-MIR-1915-3P	99.58	66.79	1988
HSA-MIR-1207-5P	99.49	69.11	2983
HSA-MIR-6081	99.48	66.07	1446
HSA-MIR-3182	99.40	68.15	2454
HSA-MIR-5580-5P	99.38	66.96	1139
HSA-MIR-4505	99.27	67.81	2678
HSA-MIR-5787	99.22	67.86	2628
HSA-MIR-485-5P	99.10	64.78	1889
HSA-MIR-6884-5P	99.10	64.50	1987
HSA-MIR-4763-3P	99.10	67.83	2649
HSA-MIR-511-5P	98.97	70.94	2268
HSA-MIR-6846-5P	98.81	65.86	1121
HSA-MIR-6848-5P	98.81	65.49	1126
HSA-MIR-1178-3P	98.57	67.09	890
HSA-MIR-518C-5P	98.53	69.20	1640
HSA-MIR-4468	98.01	66.85	1187
HSA-MIR-6748-3P	97.20	65.66	836
HSA-MIR-939-5P	97.10	65.80	1579
HSA-MIR-505-5P	97.01	65.54	778
HSA-MIR-3059-3P	96.71	67.08	606

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mrgprf	ENSMUSG00000031070
rattus_norvegicus	Mrgprf	ENSRNOG00000013426

Paralogs (10): MAS1 (ENSG00000130368), MRGPRX1 (ENSG00000170255), MRGPRD (ENSG00000172938), GPR152 (ENSG00000175514), MRGPRX4 (ENSG00000179817), MRGPRX3 (ENSG00000179826), MRGPRG (ENSG00000182170), MRGPRX2 (ENSG00000183695), MRGPRE (ENSG00000184350), MAS1L (ENSG00000204687)

Protein

Protein identifiers

Mas-related G-protein coupled receptor member F — Q96AM1 (reviewed: Q96AM1)

Alternative names: G-protein coupled receptor 140, G-protein coupled receptor 168

All UniProt accessions (2): Q96AM1, F5H5R4

UniProt curated annotations — full annotation on UniProt →

Function. Orphan receptor. May bind to a neuropeptide and may regulate nociceptor function and/or development, including the sensation or modulation of pain.

Subcellular location. Cell membrane.

Similarity. Belongs to the G-protein coupled receptor 1 family. Mas subfamily.

RefSeq proteins (2): NP_001091985, NP_659452* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000276	GPCR_Rhodpsn	Family
IPR017452	GPCR_Rhodpsn_7TM	Domain
IPR026228	MRGPCRF	Family
IPR026234	MRGPCRFAMILY	Family

Pfam: PF00001

UniProt features (19 total): topological domain 8, transmembrane region 7, chain 1, region of interest 1, glycosylation site 1, sequence variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q96AM1-F1	80.58	0.52

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Glycosylation sites (1): 4

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 96 (showing top): GSE45365_CTRL_VS_MCMV_INFECTION_NK_CELL_UP, RNGTGGGC_UNKNOWN, AP1_01, FREAC2_01, WWTAAGGC_UNKNOWN, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_DN, CHANDRAN_METASTASIS_DN, chr11q13, CAGCTG_AP4_Q5, MONNIER_POSTRADIATION_TUMOR_ESCAPE_UP, SRF_Q5_01, CREB_Q4, SRF_C, FREAC3_01, HEN1_01

GO Biological Process (2): G protein-coupled receptor signaling pathway (GO:0007186), signal transduction (GO:0007165)

GO Molecular Function (1): G protein-coupled receptor activity (GO:0004930)

GO Cellular Component (5): nucleoplasm (GO:0005654), plasma membrane (GO:0005886), nuclear membrane (GO:0031965), ciliary transition zone (GO:0035869), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
G protein-coupled receptor activity	1
signal transduction	1
cell communication	1
cellular process	1
signaling	1
regulation of cellular process	1
cellular response to stimulus	1
transmembrane signaling receptor activity	1
G protein-coupled receptor signaling pathway	1
nuclear lumen	1
membrane	1
cell periphery	1
nucleus	1
nuclear envelope	1
organelle membrane	1
cilium	1

Protein interactions and networks

STRING

832 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
MRGPRF	NPFF	O15130	822
MRGPRF	OR7C2	O60412	507
MRGPRF	COPZ2	Q9P299	458
MRGPRF	ZDHHC13	Q8IUH4	441
MRGPRF	OR5C1	Q8NGR4	440
MRGPRF	MYEOV	Q96EZ4	435
MRGPRF	VGLL3	A8MV65	422
MRGPRF	ATOH8	Q96SQ7	421
MRGPRF	PRRX2	Q99811	415
MRGPRF	EFEMP2	O95967	402
MRGPRF	GRM3	Q14832	399
MRGPRF	FKBP10	Q96AY3	398
MRGPRF	COL16A1	Q07092	398
MRGPRF	COL1A2	P02464	388
MRGPRF	TGFB1I1	O43294	388

IntAct

6 interactions, top by confidence:

A	B	Type	Score
MRGPRF	RAMP3	psi-mi:“MI:0915”(physical association)	0.400
	ESYT2	psi-mi:“MI:0914”(association)	0.350
E5	ESYT2	psi-mi:“MI:0914”(association)	0.350

BioGRID (8): MRGPRF (Affinity Capture-MS), MRGPRF (Affinity Capture-MS), MRGPRF (Affinity Capture-MS), MRGPRF (Affinity Capture-MS), USP45 (Affinity Capture-Western), MRGPRF (Affinity Capture-Western), MRGPRF (PCA), MRGPRF (Reconstituted Complex)

ESM2 similar proteins: P04201, P12526, P23749, P30554, P35410, P59534, P59535, Q3KNA1, Q3UG50, Q3UG61, Q4VHE7, Q645S5, Q645U4, Q645Y7, Q645Y8, Q645Y9, Q646A9, Q646B0, Q646C8, Q646D3, Q646F0, Q646F1, Q67ER9, Q67ES7, Q67ET0, Q67ET2, Q7TN38, Q7TN41, Q7TN44, Q7TN51, Q7TQA5, Q7TQA6, Q7TQB8, Q8CIP3, Q8R4G1, Q8TDS7, Q8VCJ6, Q91WW2, Q91WW3, Q91ZB8

Diamond homologs: B9VR26, O55197, O88680, P04201, P12526, P23749, P30554, P35410, Q16581, Q2LL16, Q3KNA1, Q3UG50, Q3UG61, Q4QXU0, Q4QXU2, Q4QXU3, Q4QXU4, Q4QXU5, Q4QXU6, Q4QXX9, Q5REI5, Q5U9D9, Q6L786, Q6TAC8, Q6XKD3, Q7TN38, Q7TN39, Q7TN40, Q7TN41, Q7TN44, Q7TN45, Q7TN49, Q7TN50, Q7TN51, Q86SM5, Q8CIP3, Q8NGA4, Q8R4G1, Q8TDS7, Q8VCJ6

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

75 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	66
Likely benign	2
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

459 predictions. Top by Δscore:

Variant	Effect	Δscore
11:69006257:CACAT:C	acceptor_gain	1.0000
11:69006259:CAT:C	acceptor_gain	1.0000
11:69006262:CT:C	acceptor_loss	1.0000
11:69009953:CCCAC:C	acceptor_gain	1.0000
11:69009954:CCACC:C	acceptor_gain	1.0000
11:69009956:ACCTG:A	acceptor_loss	1.0000
11:69009958:C:CA	acceptor_loss	1.0000
11:69006258:ACAT:A	acceptor_gain	0.9900
11:69006259:CATC:C	acceptor_gain	0.9900
11:69006260:AT:A	acceptor_gain	0.9900
11:69006262:C:CC	acceptor_gain	0.9900
11:69006265:C:CT	acceptor_gain	0.9900
11:69006267:CAGG:C	acceptor_gain	0.9900
11:69006270:G:C	acceptor_gain	0.9900
11:69006270:G:GC	acceptor_gain	0.9900
11:69009849:CTTA:C	donor_loss	0.9900
11:69009850:TTACC:T	donor_loss	0.9900
11:69009851:TA:T	donor_loss	0.9900
11:69009852:ACC:A	donor_loss	0.9900
11:69009955:CAC:C	acceptor_gain	0.9900
11:69009962:C:CT	acceptor_gain	0.9900
11:69009963:A:T	acceptor_gain	0.9900
11:69009970:C:CT	acceptor_gain	0.9900
11:69012412:A:AC	donor_gain	0.9900
11:69012413:C:CC	donor_gain	0.9900
11:69013078:GCTAC:G	donor_loss	0.9900
11:69013079:CTACC:C	donor_loss	0.9900
11:69013080:TACCT:T	donor_loss	0.9900
11:69013081:ACCT:A	donor_loss	0.9900
11:69013082:C:G	donor_loss	0.9900

AlphaMissense

2221 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
11:69005426:C:T	G295E	0.989
11:69006138:C:G	G58R	0.988
11:69005375:C:G	R312P	0.987
11:69005447:G:C	P288R	0.986
11:69005447:G:T	P288H	0.986
11:69005475:A:G	C279R	0.986
11:69005808:A:G	W168R	0.986
11:69005808:A:T	W168R	0.986
11:69005439:A:C	Y291D	0.984
11:69005455:G:C	S285R	0.984
11:69005455:G:T	S285R	0.984
11:69005457:T:G	S285R	0.984
11:69005566:G:C	F248L	0.984
11:69005566:G:T	F248L	0.984
11:69005568:A:G	F248L	0.984
11:69006147:A:G	C55R	0.984
11:69005441:A:T	V290D	0.983
11:69006025:G:C	S95R	0.983
11:69006025:G:T	S95R	0.983
11:69006027:T:G	S95R	0.983
11:69006137:C:T	G58D	0.983
11:69005427:C:G	G295R	0.981
11:69005427:C:T	G295R	0.981
11:69005453:G:T	A286D	0.981
11:69005469:A:G	C281R	0.981
11:69005896:G:C	S138R	0.981
11:69005896:G:T	S138R	0.981
11:69005898:T:G	S138R	0.981
11:69006124:G:C	N62K	0.981
11:69006124:G:T	N62K	0.981

dbSNP variants (sampled 300 via entrez): RS1000005074 (11:69014742 G>A,T), RS1000439978 (11:69015004 A>G), RS1000737094 (11:69012962 C>T), RS1000849518 (11:69011059 G>C), RS1001530743 (11:69008675 G>A,C), RS1001563367 (11:69008934 A>T), RS1002094691 (11:69013588 C>T), RS1002137097 (11:69007559 A>T), RS1002170229 (11:69011932 C>A,T), RS1002836416 (11:69013924 C>A,T), RS1003238970 (11:69007706 T>G), RS1003367817 (11:69012683 A>T), RS1003407068 (11:69008848 G>A), RS1003846107 (11:69008618 C>T), RS1004247755 (11:69009134 G>A)

Disease associations

OMIM: gene MIM:607233 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL4523903 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: gpcr — Class A Orphans with no pharmacology

CTD chemical–gene interactions

22 total (human), top 22 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	affects cotreatment, increases expression, decreases expression, increases methylation	4
entinostat	increases expression, affects cotreatment	2
Phenylmercuric Acetate	affects cotreatment, increases expression	2
methylmercuric chloride	increases expression	1
pirinixic acid	increases expression, affects binding, increases activity	1
sodium arsenite	increases expression, increases abundance	1
mercuric bromide	increases expression, affects cotreatment	1
perfluorooctane sulfonic acid	decreases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, increases expression	1
dorsomorphin	affects cotreatment, increases expression	1
Resveratrol	affects cotreatment, decreases expression	1
Temozolomide	decreases expression	1
Air Pollutants	decreases expression, increases abundance	1
Arsenic	increases abundance, increases expression	1
Benzo(a)pyrene	affects methylation, increases methylation	1
Carbamazepine	affects expression	1
Dexamethasone	increases expression	1
Estradiol	increases expression	1
Plant Extracts	affects cotreatment, decreases expression	1
Triclosan	decreases expression	1
Cadmium Chloride	increases expression	1
Particulate Matter	decreases expression, increases abundance	1

ChEMBL screening assays

2 unique, capped per target: 2 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4883533	Binding	PRESTO-Tango GPCRome screening (MRGPRF)	Data for DCP probe UCSF924

Cellosaurus cell lines

1 cell lines: 1 spontaneously immortalized cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_KY47	PathHunter CHO-K1 MRGPRF beta-arrestin	Spontaneously immortalized cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.