MRPL9

Summary

MRPL9 (mitochondrial ribosomal protein L9, HGNC:14277) is a protein-coding gene on chromosome 1q21.3, encoding Large ribosomal subunit protein bL9m (Q9BYD2). It is a selective cancer dependency (DepMap: 40.1% of cell lines).

This is a nuclear gene encoding a protein component of the 39S subunit of the mitochondrial ribosome. Alternative splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.

Source: NCBI Gene 65005 — RefSeq curated summary.

At a glance

• GWAS associations: 2
• Clinical variants (ClinVar): 70 total
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 40.1% of screened cell lines
• MANE Select transcript: NM_031420

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:14277
Approved symbol	MRPL9
Name	mitochondrial ribosomal protein L9
Location	1q21.3
Locus type	gene with protein product
Status	Approved
Aliases	bL9m
Ensembl gene	ENSG00000143436
Ensembl biotype	protein_coding
OMIM	611824
Entrez	65005

Gene structure

Transcript identifiers

Ensembl transcripts: 11 — 4 nonsense_mediated_decay, 3 protein_coding, 2 retained_intron, 2 protein_coding_CDS_not_defined

ENST00000368829, ENST00000368830, ENST00000461182, ENST00000462783, ENST00000467306, ENST00000468006, ENST00000478926, ENST00000481777, ENST00000486707, ENST00000492684, ENST00000495867

RefSeq mRNA: 2 — MANE Select: NM_031420 NM_001300733, NM_031420

CCDS: CCDS1003, CCDS72916

Canonical transcript exons

ENST00000368830 — 7 exons

Exon	Start	End
ENSE00001819085	151759647	151760181
ENSE00001941155	151763327	151763496
ENSE00003533593	151760816	151760899
ENSE00003544764	151762105	151762155
ENSE00003568110	151762990	151763146
ENSE00003585016	151761451	151761552
ENSE00003674457	151762376	151762500

Expression profiles

Bgee: expression breadth ubiquitous, 295 present calls, max score 97.90.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 60.5274 / max 363.7967, expressed in 1822 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
14458	59.5252	1821
14457	1.0021	420

Top tissues by expression

295 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
endometrium epithelium	UBERON:0004811	97.90	gold quality
parotid gland	UBERON:0001831	97.35	gold quality
ganglionic eminence	UBERON:0004023	96.96	gold quality
ventricular zone	UBERON:0003053	96.94	gold quality
hindlimb stylopod muscle	UBERON:0004252	96.82	gold quality
right lobe of thyroid gland	UBERON:0001119	96.51	gold quality
left lobe of thyroid gland	UBERON:0001120	96.39	gold quality
cervix squamous epithelium	UBERON:0006922	96.36	gold quality
thyroid gland	UBERON:0002046	96.11	gold quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	95.86	gold quality
skin of abdomen	UBERON:0001416	95.71	gold quality
cerebellar cortex	UBERON:0002129	95.68	gold quality
cerebellar hemisphere	UBERON:0002245	95.67	gold quality
body of uterus	UBERON:0009853	95.60	gold quality
right hemisphere of cerebellum	UBERON:0014890	95.58	gold quality
metanephros cortex	UBERON:0010533	95.52	gold quality
body of pancreas	UBERON:0001150	95.49	gold quality
tendon of biceps brachii	UBERON:0008188	95.49	gold quality
skin of leg	UBERON:0001511	95.47	gold quality
prostate gland	UBERON:0002367	95.44	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	95.44	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	95.43	gold quality
biceps brachii	UBERON:0001507	95.42	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	95.40	gold quality
vastus lateralis	UBERON:0001379	95.40	gold quality
spleen	UBERON:0002106	95.36	gold quality
mucosa of transverse colon	UBERON:0004991	95.33	gold quality
right lung	UBERON:0002167	95.30	gold quality
muscle layer of sigmoid colon	UBERON:0035805	95.28	gold quality
embryo	UBERON:0000922	95.27	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

25 targeting MRPL9, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-432-3P	100.00	67.86	705
HSA-MIR-1193	100.00	65.93	529
HSA-MIR-3662	99.99	73.82	5684
HSA-MIR-6809-3P	99.91	71.45	3814
HSA-MIR-4753-3P	99.90	71.03	3786
HSA-MIR-3133	99.81	70.92	3506
HSA-MIR-4645-3P	99.76	69.33	993
HSA-MIR-6752-3P	99.72	66.71	1587
HSA-MIR-4762-5P	99.57	68.54	1424
HSA-MIR-217-5P	99.49	69.93	1419
HSA-MIR-766-5P	99.47	67.91	2225
HSA-MIR-520F-5P	99.34	70.40	1632
HSA-MIR-10522-5P	99.26	68.50	2087
HSA-MIR-4426	99.17	66.74	1949
HSA-MIR-6071	99.16	67.77	1780
HSA-MIR-6807-3P	99.15	69.23	1275
HSA-MIR-1843	98.97	66.07	838
HSA-MIR-4802-5P	98.97	66.26	833
HSA-MIR-4272	98.76	68.74	1810
HSA-MIR-4742-3P	98.73	69.82	1803
HSA-MIR-4646-3P	98.65	66.98	693
HSA-MIR-2117	98.48	67.97	1307
HSA-MIR-4778-5P	97.96	68.06	1634
HSA-MIR-6828-3P	96.06	67.61	1155
HSA-MIR-4433B-5P	95.91	66.56	727

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 40.1% of screened cell lines.

Literature-anchored findings (GeneRIF, showing 2)

• Interaction of MRPL9 and GGCT Promotes Cell Proliferation and Migration by Activating the MAPK/ERK Pathway in Papillary Thyroid Cancer. (PMID:36233293)
• Identification of a novel therapeutic target for lung cancer: Mitochondrial ribosome protein L9. (PMID:37343379)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	mrpl9	ENSDARG00000063249
mus_musculus	Mrpl9	ENSMUSG00000028140
rattus_norvegicus	mrpl9	ENSRNOG00000020869
drosophila_melanogaster	mRpL9	FBGN0038319
caenorhabditis_elegans	mrpl-9	WBGENE00015025

Protein

Protein identifiers

Large ribosomal subunit protein bL9m — Q9BYD2 (reviewed: Q9BYD2)

Alternative names: 39S ribosomal protein L9, mitochondrial

All UniProt accessions (7): Q9BYD2, A0A1W2PPE5, A0A1W2PPK1, A0A1W2PQB6, A0A1W2PRK9, A0A1W2PS92, Q5SZR1

UniProt curated annotations — full annotation on UniProt →

Subunit / interactions. Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins.

Subcellular location. Mitochondrion.

Similarity. Belongs to the bacterial ribosomal protein bL9 family.

RefSeq proteins (2): NP_001287662, NP_113608* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000244	Ribosomal_bL9	Family
IPR009027	Ribosomal_bL9/RNase_H1_N	Homologous_superfamily
IPR020070	Ribosomal_bL9_N	Domain
IPR036935	Ribosomal_bL9_N_sf	Homologous_superfamily
IPR054302	Ribosomal_bL9m_C	Domain
IPR056864	MRP-L9_N	Domain

Pfam: PF01281, PF22078, PF25131

UniProt features (17 total): strand 7, helix 4, turn 2, sequence variant 2, transit peptide 1, chain 1

Structure

Experimental structures (PDB)

86 structures, top 30 by resolution.

PDB	Method	Resolution (Å)
7OF0	ELECTRON MICROSCOPY	2.2
7QI4	ELECTRON MICROSCOPY	2.21
8RRI	ELECTRON MICROSCOPY	2.4
8QU5	ELECTRON MICROSCOPY	2.42
9OLF	ELECTRON MICROSCOPY	2.46
7OF7	ELECTRON MICROSCOPY	2.5
7PO4	ELECTRON MICROSCOPY	2.56
6ZM6	ELECTRON MICROSCOPY	2.59
7O9M	ELECTRON MICROSCOPY	2.6
7OF6	ELECTRON MICROSCOPY	2.6
9CN3	ELECTRON MICROSCOPY	2.62
7QI5	ELECTRON MICROSCOPY	2.63
7OF2	ELECTRON MICROSCOPY	2.7
7OF3	ELECTRON MICROSCOPY	2.7
7OF4	ELECTRON MICROSCOPY	2.7
8QU1	ELECTRON MICROSCOPY	2.74
9PR4	ELECTRON MICROSCOPY	2.77
9PRA	ELECTRON MICROSCOPY	2.83
8ANY	ELECTRON MICROSCOPY	2.85
6ZM5	ELECTRON MICROSCOPY	2.89
7QH7	ELECTRON MICROSCOPY	2.89
7ODR	ELECTRON MICROSCOPY	2.9
7OF5	ELECTRON MICROSCOPY	2.9
8K2A	ELECTRON MICROSCOPY	2.9
8OIT	ELECTRON MICROSCOPY	2.9
9PGL	ELECTRON MICROSCOPY	2.9
9PGF	ELECTRON MICROSCOPY	2.93
6VMI	ELECTRON MICROSCOPY	2.96
6VLZ	ELECTRON MICROSCOPY	2.97
7QI6	ELECTRON MICROSCOPY	2.98

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9BYD2-F1	82.36	0.61

Function

Pathways and Gene Ontology

Reactome pathways

7 pathways

ID	Pathway
R-HSA-5368286	Mitochondrial translation initiation
R-HSA-5389840	Mitochondrial translation elongation
R-HSA-5419276	Mitochondrial translation termination
R-HSA-9937383	Mitochondrial ribosome-associated quality control
R-HSA-392499	Metabolism of proteins
R-HSA-5368287	Mitochondrial translation
R-HSA-72766	Translation

MSigDB gene sets: 171 (showing top): GSE45365_NK_CELL_VS_CD8A_DC_MCMV_INFECTION_DN, MORF_MTA1, MORF_UBE2I, MORF_HDAC1, MORF_UBE2N, GOBP_MITOCHONDRIAL_TRANSLATION, MORF_RAD21, MORF_HDAC2, VETTER_TARGETS_OF_PRKCA_AND_ETS1_DN, GOBP_TRANSLATION, MORF_SKP1A, MORF_RAF1, MORF_CCNI, MORF_CTBP1, MORF_RAB6A

GO Biological Process (2): translation (GO:0006412), mitochondrial translation (GO:0032543)

GO Molecular Function (3): RNA binding (GO:0003723), structural constituent of ribosome (GO:0003735), protein binding (GO:0005515)

GO Cellular Component (6): mitochondrion (GO:0005739), mitochondrial inner membrane (GO:0005743), mitochondrial ribosome (GO:0005761), mitochondrial large ribosomal subunit (GO:0005762), ribosome (GO:0005840), ribonucleoprotein complex (GO:1990904)

Reactome top-level categories

Rollup of top-3 pathways:

Category	Pathways
Mitochondrial translation	4
Translation	1
Metabolism of proteins	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
peptidyltransferase activity	1
translational initiation	1
translational elongation	1
translational termination	1
macromolecule biosynthetic process	1
protein metabolic process	1
protein biosynthetic process	1
mitochondrion	1
translation	1
mitochondrial gene expression	1
nucleic acid binding	1
structural molecule activity	1
ribosome	1
binding	1
cytoplasm	1
intracellular membrane-bounded organelle	1
organelle inner membrane	1
mitochondrial membrane	1
organellar ribosome	1
mitochondrial matrix	1
organellar large ribosomal subunit	1
mitochondrial ribosome	1
mitochondrial protein-containing complex	1
intracellular membraneless organelle	1
protein-containing complex	1

Protein interactions and networks

STRING

2153 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
MRPL9	MRPL47	Q9HD33	661
MRPL9	MRPS10	P82664	614
MRPL9	MRPL24	Q96A35	604
MRPL9	MRPL21	Q7Z2W9	595
MRPL9	MRPL19	P49406	589
MRPL9	MRPL23	Q16540	585
MRPL9	A8MYK1	A8MYK1	556
MRPL9	MRPL13	Q9BYD1	551
MRPL9	MRPL17	Q9NRX2	542
MRPL9	MRPL11	Q9Y3B7	540
MRPL9	MRPL12	P52815	535
MRPL9	MRPL2	Q5T653	534
MRPL9	MRPL36	Q9P0J6	530
MRPL9	MRPL20	Q9BYC9	521
MRPL9	MRPL3	P09001	515

IntAct

194 interactions, top by confidence:

A	B	Type	Score
YBX1	HNRNPR	psi-mi:“MI:0914”(association)	0.770
EZH2	EPOP	psi-mi:“MI:0914”(association)	0.730
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
MRPL37	HSPD1	psi-mi:“MI:0914”(association)	0.710
MALSU1	NDUFAB1	psi-mi:“MI:0914”(association)	0.710
MRPS30	GTPBP10	psi-mi:“MI:0914”(association)	0.640
LIN28A	IGF2BP3	psi-mi:“MI:0914”(association)	0.640
FAM9B	MRPL9	psi-mi:“MI:0915”(physical association)	0.560
MRPL9	FAM9B	psi-mi:“MI:0915”(physical association)	0.560
HNRNPK	MRPL9	psi-mi:“MI:0915”(physical association)	0.560
CDC37	MRPL9	psi-mi:“MI:0915”(physical association)	0.560
NP	KPNA6	psi-mi:“MI:0914”(association)	0.550
MRPL50	GTPBP10	psi-mi:“MI:0914”(association)	0.530
MRPL10	ZZEF1	psi-mi:“MI:0914”(association)	0.530
MRPL42	GATC	psi-mi:“MI:0914”(association)	0.530
MRPL28	MRPL3	psi-mi:“MI:0914”(association)	0.530
MRPL41	MRPL3	psi-mi:“MI:0914”(association)	0.530
MRPL2	GTPBP10	psi-mi:“MI:0914”(association)	0.530
THAP3	CASC3	psi-mi:“MI:0914”(association)	0.530
H1-4	RRP8	psi-mi:“MI:0914”(association)	0.530
HNRNPA1	PTCD1	psi-mi:“MI:0914”(association)	0.530
IGF2BP3	PTCD1	psi-mi:“MI:0914”(association)	0.530
MRPL13	GTPBP10	psi-mi:“MI:0914”(association)	0.530
MRPL18	GTPBP10	psi-mi:“MI:0914”(association)	0.530
ABT1	ZNF316	psi-mi:“MI:0914”(association)	0.530

BioGRID (306): FAM9B (Two-hybrid), MRPL9 (Affinity Capture-MS), MRPL9 (Affinity Capture-MS), MRPL9 (Affinity Capture-MS), MRPL9 (Affinity Capture-MS), MRPL9 (Affinity Capture-MS), MRPL9 (Affinity Capture-MS), MRPL9 (Affinity Capture-MS), MRPL9 (Affinity Capture-MS), MRPL9 (Affinity Capture-MS), MRPL9 (Affinity Capture-MS), MRPL9 (Affinity Capture-MS), MRPL9 (Affinity Capture-MS), MRPL9 (Affinity Capture-MS), MRPL9 (Affinity Capture-MS)

ESM2 similar proteins: A6QPQ5, A9X1A9, O95707, P0C2C1, P82664, P82670, P82675, P82915, P82919, P82933, Q08BI9, Q0P5E7, Q0VFH6, Q2KIB9, Q2KID9, Q2KIJ6, Q2TBK2, Q2TBR2, Q3MHY7, Q3SYS0, Q3T040, Q58DQ5, Q58DV5, Q5C9Z4, Q5R7B0, Q5REJ1, Q5REY4, Q5RFM3, Q641X9, Q7Z2W9, Q7Z7H8, Q8N3Z3, Q8TCC3, Q924T2, Q99N85, Q99N87, Q99N94, Q9BSH4, Q9BYD2, Q9BYD6

Diamond homologs: A0LAG5, A0LWU2, A0M0D9, A0Q6H2, A0Q8Y8, A0R7F6, A1AM21, A1SA57, A1THY4, A1U390, A1US53, A1WUS4, A3D8Q2, A3QI49, A4QI23, A4T4P0, A4VQM7, A4XQ00, A5FE75, A5FYN6, A5W9R5, A6H0P1, A6KWD5, A6WJ82, A7H6J2, A8FR98, A8H8K9, A9B527, A9IRT7, A9L123, A9X1A9, B0K5L4, B0K8F8, B0KKY0, B0RUY7, B0RZP8, B0TUU6, B0U5H0, B1MMH9, B1XN09

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
MRPL9	“form complex”	“39S mitochondrial large ribosomal subunit”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 156 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Mitochondrial ribosome-associated quality control	39	39.9×	5e-52
Mitochondrial translation	33	37.8×	5e-43
Mitochondrial translation initiation	35	37.0×	3e-45
Mitochondrial translation elongation	35	37.0×	3e-45
Mitochondrial translation termination	35	32.0×	8e-43
Translation	36	18.6×	1e-34
Transport of Mature Transcript to Cytoplasm	5	15.9×	2e-04
Peptide chain elongation	14	14.8×	2e-11

GO biological processes:

GO term	Partners	Fold	FDR
mitochondrial translation	38	45.5×	1e-51
mitochondrial large ribosomal subunit assembly	5	34.2×	5e-05
translation	32	22.7×	4e-32
cytoplasmic translation	14	17.9×	1e-11
regulation of alternative mRNA splicing, via spliceosome	7	11.8×	3e-04
negative regulation of translation	7	9.5×	1e-03
mRNA processing	9	4.9×	8e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

70 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	36
Likely benign	4
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

915 predictions. Top by Δscore:

Variant	Effect	Δscore
1:151760811:CTCA:C	donor_loss	1.0000
1:151760812:TCA:T	donor_loss	1.0000
1:151760813:CA:C	donor_loss	1.0000
1:151760814:A:AC	donor_gain	1.0000
1:151760814:A:AG	donor_loss	1.0000
1:151760815:C:A	donor_loss	1.0000
1:151760815:C:CC	donor_gain	1.0000
1:151760815:CCGT:C	donor_gain	1.0000
1:151760895:CCAAG:C	acceptor_gain	1.0000
1:151760896:CAAG:C	acceptor_gain	1.0000
1:151760896:CAAGC:C	acceptor_gain	1.0000
1:151760897:AAG:A	acceptor_gain	1.0000
1:151760898:AG:A	acceptor_gain	1.0000
1:151760899:GC:G	acceptor_loss	1.0000
1:151760900:C:CC	acceptor_gain	1.0000
1:151761445:A:AC	donor_gain	1.0000
1:151761446:C:CC	donor_gain	1.0000
1:151761446:CT:C	donor_gain	1.0000
1:151761446:CTCA:C	donor_gain	1.0000
1:151761449:A:AC	donor_gain	1.0000
1:151761450:C:CC	donor_gain	1.0000
1:151761450:CATT:C	donor_gain	1.0000
1:151761549:CTGT:C	acceptor_gain	1.0000
1:151762151:CTCAG:C	acceptor_gain	1.0000
1:151762153:CAG:C	acceptor_gain	1.0000
1:151762155:GCTAG:G	acceptor_loss	1.0000
1:151762156:C:CA	acceptor_loss	1.0000
1:151762156:C:CC	acceptor_gain	1.0000
1:151762362:T:TA	donor_gain	1.0000
1:151762370:GCTCA:G	donor_loss	1.0000

AlphaMissense

1692 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:151761473:A:T	V189D	0.995
1:151761495:A:G	W182R	0.994
1:151761495:A:T	W182R	0.994
1:151760144:A:T	V237D	0.993
1:151761493:C:A	W182C	0.991
1:151761493:C:G	W182C	0.991
1:151760827:A:G	C221R	0.990
1:151760886:A:T	V201D	0.989
1:151762425:G:T	A129D	0.988
1:151763013:A:G	L96P	0.988
1:151763013:A:T	L96H	0.988
1:151761539:A:G	L167P	0.984
1:151760156:A:T	V233E	0.983
1:151761467:C:G	R191P	0.983
1:151762452:C:G	R120P	0.982
1:151760825:A:C	C221W	0.981
1:151760134:A:C	F240L	0.980
1:151760134:A:T	F240L	0.980
1:151760136:A:G	F240L	0.980
1:151760180:A:T	V225E	0.979
1:151762455:C:T	G119D	0.979
1:151760892:A:T	V199D	0.977
1:151763007:A:T	L98Q	0.976
1:151761494:C:A	W182L	0.975
1:151760820:A:T	V223E	0.974
1:151761524:A:G	L172P	0.974
1:151761539:A:T	L167Q	0.974
1:151763128:G:T	R58S	0.974
1:151763062:A:C	Y80D	0.973
1:151760150:A:T	M235K	0.971

dbSNP variants (sampled 300 via entrez): RS1000681460 (1:151761067 T>C), RS1001020223 (1:151759515 A>C), RS1001105807 (1:151765278 T>C), RS1001130916 (1:151764478 C>G,T), RS1001159552 (1:151764943 C>T), RS1001225777 (1:151764007 G>C), RS1002357469 (1:151759484 ACAT>A), RS1003456080 (1:151761349 G>A,C), RS1003809841 (1:151761658 A>G), RS1004579396 (1:151761766 G>C), RS1004637141 (1:151761821 A>C), RS1004990215 (1:151761500 A>C), RS1005486035 (1:151763218 C>A,T), RS1005807516 (1:151764754 A>AGAT), RS1006249179 (1:151759918 G>A,C)

Disease associations

OMIM: gene MIM:611824 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

Study	Trait	p-value
GCST008916_130	Asthma	1.000000e-12
GCST008916_88	Asthma	1.000000e-25

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067282 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.02	Kd	9584	nM	CHEMBL3752910
5.02	ED50	9584	nM	CHEMBL3752910

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149973: Binding affinity to human MRPL9 incubated for 45 mins by Kinobead based pull down assay	kd	9.5843	uM

CTD chemical–gene interactions

21 total (human), top 21 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Acetaminophen	decreases expression	2
Tretinoin	affects cotreatment, decreases expression	2
Valproic Acid	decreases expression, affects expression	2
aristolochic acid I	increases expression	1
bisphenol A	decreases expression	1
arsenite	affects binding, increases reaction	1
sodium arsenite	affects cotreatment, decreases expression	1
cylindrospermopsin	increases expression	1
bisphenol B	increases expression	1
STA 9090	decreases expression	1
bisphenol AF	increases expression	1
Atrazine	decreases expression	1
Carbamazepine	affects expression	1
Ivermectin	decreases expression	1
Lead	affects expression	1
Ribonucleotides	affects binding	1
Selenium	increases expression	1
Smoke	decreases expression	1
Vitamin E	increases expression	1
Antirheumatic Agents	increases expression	1
Particulate Matter	increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5653015	Binding	Binding affinity to human MRPL9 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.