MRPS17

Summary

MRPS17 (mitochondrial ribosomal protein S17, HGNC:14047) is a protein-coding gene on chromosome 7p11.2, encoding Small ribosomal subunit protein uS17m (Q9Y2R5). It is a selective cancer dependency (DepMap: 15.1% of cell lines).

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S17P family. The encoded protein is moderately conserved between human mitochondrial and prokaryotic ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 1p, 3p, 6q, 14p, 18q, and Xq.

Source: NCBI Gene 51373 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 22 total
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 15.1% of screened cell lines
• MANE Select transcript: NM_015969

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:14047
Approved symbol	MRPS17
Name	mitochondrial ribosomal protein S17
Location	7p11.2
Locus type	gene with protein product
Status	Approved
Aliases	HSPC011, RPMS17, MRP-S17, uS17m
Ensembl gene	ENSG00000239789
Ensembl biotype	protein_coding
OMIM	611980
Entrez	51373

Gene structure

Transcript identifiers

Ensembl transcripts: 16 — 16 protein_coding

ENST00000285298, ENST00000443449, ENST00000909935, ENST00000909936, ENST00000914644, ENST00000914645, ENST00000914646, ENST00000914647, ENST00000914648, ENST00000914649, ENST00000914650, ENST00000914651, ENST00000914652, ENST00000914653, ENST00000914654, ENST00000943621

RefSeq mRNA: 1 — MANE Select: NM_015969 NM_015969

CCDS: CCDS5520

Canonical transcript exons

ENST00000285298 — 3 exons

Exon	Start	End
ENSE00001175066	55954909	55956500
ENSE00001596608	55951877	55951930
ENSE00003675267	55953179	55953318

Expression profiles

Bgee: expression breadth ubiquitous, 139 present calls, max score 89.71.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 20.9950 / max 208.2075, expressed in 1801 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
78763	20.6332	1801
78764	0.3618	194

Top tissues by expression

139 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
gastrocnemius	UBERON:0001388	89.71	gold quality
hindlimb stylopod muscle	UBERON:0004252	89.24	gold quality
muscle of leg	UBERON:0001383	89.21	gold quality
skeletal muscle organ	UBERON:0014892	89.19	gold quality
skeletal muscle tissue	UBERON:0001134	88.58	gold quality
muscle tissue	UBERON:0002385	86.81	gold quality
heart left ventricle	UBERON:0002084	86.59	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	86.33	gold quality
mucosa of transverse colon	UBERON:0004991	85.94	gold quality
islet of Langerhans	UBERON:0000006	85.45	gold quality
heart	UBERON:0000948	84.76	gold quality
prefrontal cortex	UBERON:0000451	84.50	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	84.25	gold quality
lower esophagus	UBERON:0013473	84.18	gold quality
lower esophagus muscularis layer	UBERON:0035833	84.18	gold quality
right lobe of liver	UBERON:0001114	84.02	gold quality
frontal cortex	UBERON:0001870	83.89	gold quality
right atrium auricular region	UBERON:0006631	83.79	gold quality
pancreas	UBERON:0001264	83.73	gold quality
cerebral cortex	UBERON:0000956	83.64	gold quality
Brodmann (1909) area 9	UBERON:0013540	83.64	gold quality
anterior cingulate cortex	UBERON:0009835	83.52	gold quality
right adrenal gland	UBERON:0001233	83.43	gold quality
apex of heart	UBERON:0002098	83.31	gold quality
left adrenal gland cortex	UBERON:0035825	83.29	gold quality
left adrenal gland	UBERON:0001234	83.27	gold quality
right frontal lobe	UBERON:0002810	83.24	gold quality
cortical plate	UBERON:0005343	83.20	gold quality
body of pancreas	UBERON:0001150	83.15	gold quality
nucleus accumbens	UBERON:0001882	83.07	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	8.30
E-MTAB-9689	no	291.91
E-MTAB-4850	no	139.41

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): MYCN

miRNA regulators (miRDB)

21 targeting MRPS17, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-223-3P	99.99	70.14	1140
HSA-MIR-4803	99.98	71.99	3117
HSA-MIR-548AA	99.96	70.64	3753
HSA-MIR-548AP-3P	99.96	70.64	3753
HSA-MIR-548T-3P	99.96	70.64	3753
HSA-MIR-548D-3P	99.87	70.67	4362
HSA-MIR-3919	99.87	69.45	2489
HSA-MIR-548BB-3P	99.86	70.58	4354
HSA-MIR-548AC	99.84	70.77	4351
HSA-MIR-548H-3P	99.84	70.80	4349
HSA-MIR-548Z	99.84	70.80	4349
HSA-MIR-204-5P	99.79	71.62	2439
HSA-MIR-211-5P	99.79	71.65	2440
HSA-MIR-4446-5P	99.72	69.19	2544
HSA-MIR-5006-3P	99.71	70.26	2728
HSA-MIR-4755-5P	99.71	70.34	2716
HSA-MIR-587	99.64	70.86	2611
HSA-MIR-4328	99.57	71.06	4094
HSA-MIR-4738-3P	98.98	67.98	1846
HSA-MIR-4638-3P	97.90	65.75	905
HSA-MIR-3654	96.43	66.55	646

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 15.1% of screened cell lines.

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	mrps17	ENSDARG00000054164
mus_musculus	Mrps17	ENSMUSG00000034211
rattus_norvegicus	Mrps17	ENSRNOG00000066020
drosophila_melanogaster	mRpS17	FBGN0034986
caenorhabditis_elegans		WBGENE00015487

Protein

Protein identifiers

Small ribosomal subunit protein uS17m — Q9Y2R5 (reviewed: Q9Y2R5)

Alternative names: 28S ribosomal protein S17, mitochondrial

All UniProt accessions (2): E9PE17, Q9Y2R5

UniProt curated annotations — full annotation on UniProt →

Subunit / interactions. Component of the mitochondrial small ribosomal subunit (mt-SSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins.

Subcellular location. Mitochondrion.

Similarity. Belongs to the universal ribosomal protein uS17 family.

RefSeq proteins (1): NP_057053* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000266	Ribosomal_uS17	Family
IPR012340	NA-bd_OB-fold	Homologous_superfamily
IPR039193	Ribosomal_uS17m_metazoa	Family

Pfam: PF00366

UniProt features (12 total): strand 7, turn 2, transit peptide 1, chain 1, helix 1

Structure

Experimental structures (PDB)

77 structures, top 30 by resolution.

PDB	Method	Resolution (Å)
7QI4	ELECTRON MICROSCOPY	2.21
8CSS	ELECTRON MICROSCOPY	2.36
7P2E	ELECTRON MICROSCOPY	2.4
8RRI	ELECTRON MICROSCOPY	2.4
9OLF	ELECTRON MICROSCOPY	2.46
9OJM	ELECTRON MICROSCOPY	2.5
8CSQ	ELECTRON MICROSCOPY	2.54
8CSR	ELECTRON MICROSCOPY	2.54
6ZM6	ELECTRON MICROSCOPY	2.59
7QI5	ELECTRON MICROSCOPY	2.63
8CSP	ELECTRON MICROSCOPY	2.66
7PNX	ELECTRON MICROSCOPY	2.76
8ANY	ELECTRON MICROSCOPY	2.85
8CST	ELECTRON MICROSCOPY	2.85
6ZM5	ELECTRON MICROSCOPY	2.89
7PO0	ELECTRON MICROSCOPY	2.9
8K2A	ELECTRON MICROSCOPY	2.9
9PGL	ELECTRON MICROSCOPY	2.9
7PO1	ELECTRON MICROSCOPY	2.92
7PO3	ELECTRON MICROSCOPY	2.92
9PGF	ELECTRON MICROSCOPY	2.93
6VMI	ELECTRON MICROSCOPY	2.96
6RW4	ELECTRON MICROSCOPY	2.97
6VLZ	ELECTRON MICROSCOPY	2.97
7QI6	ELECTRON MICROSCOPY	2.98
8QRN	ELECTRON MICROSCOPY	2.98
9PSM	ELECTRON MICROSCOPY	2.98
8OIS	ELECTRON MICROSCOPY	3
9G5C	ELECTRON MICROSCOPY	3
9G5D	ELECTRON MICROSCOPY	3

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9Y2R5-F1	93.08	0.78

Function

Pathways and Gene Ontology

Reactome pathways

7 pathways

ID	Pathway
R-HSA-5368286	Mitochondrial translation initiation
R-HSA-5389840	Mitochondrial translation elongation
R-HSA-5419276	Mitochondrial translation termination
R-HSA-9937383	Mitochondrial ribosome-associated quality control
R-HSA-392499	Metabolism of proteins
R-HSA-5368287	Mitochondrial translation
R-HSA-72766	Translation

MSigDB gene sets: 94 (showing top): RODRIGUES_THYROID_CARCINOMA_ANAPLASTIC_UP, BUYTAERT_PHOTODYNAMIC_THERAPY_STRESS_DN, STARK_PREFRONTAL_CORTEX_22Q11_DELETION_DN, GOBP_MITOCHONDRIAL_TRANSLATION, GOBP_TRANSLATION, GOMF_STRUCTURAL_CONSTITUENT_OF_RIBOSOME, GOCC_MITOCHONDRIAL_ENVELOPE, GARY_CD5_TARGETS_DN, DODD_NASOPHARYNGEAL_CARCINOMA_UP, WONG_MITOCHONDRIA_GENE_MODULE, GOCC_RIBOSOME, GOCC_SMALL_RIBOSOMAL_SUBUNIT, GOCC_ORGANELLAR_RIBOSOME, GOCC_MITOCHONDRIAL_MATRIX, GOCC_ORGANELLE_INNER_MEMBRANE

GO Biological Process (2): translation (GO:0006412), mitochondrial translation (GO:0032543)

GO Molecular Function (4): structural constituent of ribosome (GO:0003735), rRNA binding (GO:0019843), RNA binding (GO:0003723), protein binding (GO:0005515)

GO Cellular Component (5): mitochondrion (GO:0005739), mitochondrial inner membrane (GO:0005743), mitochondrial small ribosomal subunit (GO:0005763), ribosome (GO:0005840), ribonucleoprotein complex (GO:1990904)

Reactome top-level categories

Rollup of top-3 pathways:

Category	Pathways
Mitochondrial translation	4
Translation	1
Metabolism of proteins	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
peptidyltransferase activity	1
translational initiation	1
translational elongation	1
translational termination	1
macromolecule biosynthetic process	1
protein metabolic process	1
protein biosynthetic process	1
mitochondrion	1
translation	1
mitochondrial gene expression	1
structural molecule activity	1
ribosome	1
RNA binding	1
nucleic acid binding	1
binding	1
cytoplasm	1
intracellular membrane-bounded organelle	1
organelle inner membrane	1
mitochondrial membrane	1
organellar small ribosomal subunit	1
mitochondrial ribosome	1
mitochondrial protein-containing complex	1
intracellular membraneless organelle	1
protein-containing complex	1

Protein interactions and networks

STRING

1366 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
MRPS17	TUBB6	Q9BUF5	654
MRPS17	CCT6A	P40227	634
MRPS17	MRPS15	P82914	515
MRPS17	ACOT7	O00154	507
MRPS17	MRPL45	Q9BRJ2	472
MRPS17	ZNF713	Q8N859	470
MRPS17	PGAM4	Q8N0Y7	467
MRPS17	MRPL12	P52815	461
MRPS17	NIPSNAP2	O75323	442
MRPS17	MPV17L2	Q567V2	441
MRPS17	MRPS18B	Q9Y676	432
MRPS17	PGAM1	P18669	431
MRPS17	MRPL11	Q9Y3B7	422
MRPS17	P4HA1	P13674	419
MRPS17	MRPS27	Q92552	419
MRPS17	NDRG1	Q92597	419

IntAct

84 interactions, top by confidence:

A	B	Type	Score
KIFAP3	KIF3B	psi-mi:“MI:0914”(association)	0.900
MED4	MED19	psi-mi:“MI:0914”(association)	0.900
FBL	NOP56	psi-mi:“MI:0914”(association)	0.800
ERBB3	PIK3R2	psi-mi:“MI:0914”(association)	0.700
ESR1	TRIM24	psi-mi:“MI:0914”(association)	0.640
MRPS17	GRN	psi-mi:“MI:0915”(physical association)	0.560
MRPS17	WFS1	psi-mi:“MI:0915”(physical association)	0.560
NP	KPNA6	psi-mi:“MI:0914”(association)	0.550
MRPS6	STAT5A	psi-mi:“MI:0914”(association)	0.550
MRPS18B	MRPS14	psi-mi:“MI:0914”(association)	0.530
MRPS18C	MRPS14	psi-mi:“MI:0914”(association)	0.530
TFB1M	HSPD1	psi-mi:“MI:0914”(association)	0.530
MRPS15	PRKACG	psi-mi:“MI:0914”(association)	0.530
MRPS17	MRPS22	psi-mi:“MI:0914”(association)	0.530
MRPS26	MRPS10	psi-mi:“MI:0914”(association)	0.530
AURKAIP1	NRDC	psi-mi:“MI:0914”(association)	0.480
EN1	NFIB	psi-mi:“MI:2364”(proximity)	0.470
ECSIT	NDUFS2	psi-mi:“MI:0914”(association)	0.350
NP	HNRNPDL	psi-mi:“MI:0914”(association)	0.350
NP	KPNA6	psi-mi:“MI:0914”(association)	0.350
NP	TRIM66	psi-mi:“MI:0914”(association)	0.350
DLST		psi-mi:“MI:0914”(association)	0.350
DND1	RPSA2	psi-mi:“MI:0914”(association)	0.350

BioGRID (272): MRPS17 (Affinity Capture-MS), MRPS17 (Affinity Capture-MS), MRPS17 (Affinity Capture-MS), MRPS17 (Affinity Capture-MS), MRPS17 (Affinity Capture-MS), MRPS17 (Affinity Capture-MS), MRPS17 (Affinity Capture-MS), MRPS17 (Affinity Capture-MS), MRPS17 (Affinity Capture-MS), MRPS17 (Affinity Capture-MS), KIFAP3 (Affinity Capture-MS), MRPS17 (Affinity Capture-MS), MRPS17 (Affinity Capture-MS), MRPS17 (Affinity Capture-MS), MRPS17 (Affinity Capture-MS)

ESM2 similar proteins: A0A0R0IHP4, A2Y5T7, F4K6X0, O22795, O49937, O80361, O80504, O82234, P16180, P17092, P23326, P24613, P30956, P42732, P49163, P51412, P82130, P82137, P82162, P82245, P82248, P82278, P82403, P82413, P82916, P92959, Q5KQI6, Q6NPF9, Q84W65, Q8L7S9, Q8RXX5, Q8VY88, Q8VY91, Q8VZ55, Q8W463, Q94B52, Q96326, Q9ASV6, Q9FHL4, Q9FJP3

Diamond homologs: A0KRN3, A0LIJ9, A0Q4J2, A0T0I8, A0T0Y2, A1S227, A1TYK6, A1V894, A1VEA7, A2C4Z0, A2S7I5, A3MRW3, A3NEH0, A3P0A4, A3Q991, A4IZS5, A4JAP9, A4SUX0, A4VHN9, A4XZ81, A5FZV6, A5IHQ5, A5IM92, A5USI0, A6LLM2, A6UZJ7, A7N9T4, A7NR54, A8F4S0, A8G1D9, A8ZV66, A9BH96, B0RU73, B0U0Y0, B0U5K8, B1LBN1, B1XSR0, B1YRN8, B2I8H8, B2SDX6

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
MRPS17	“form complex”	“28S mitochondrial small ribosomal subunit”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 106 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Mitochondrial translation	18	31.8×	8e-21
Mitochondrial translation initiation	19	30.9×	1e-21
Mitochondrial translation elongation	19	30.9×	1e-21
Mitochondrial ribosome-associated quality control	19	29.9×	2e-21
Mitochondrial translation termination	19	26.8×	1e-20
Translation	21	16.7×	1e-18
rRNA processing	5	9.4×	8e-03
Mitochondrial protein degradation	6	8.8×	3e-03

GO biological processes:

GO term	Partners	Fold	FDR
mitochondrial translation	19	33.3×	2e-21
translation	12	12.5×	8e-08

Disease & clinical

Clinical variants and AI predictions

ClinVar

22 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	16
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

439 predictions. Top by Δscore:

Variant	Effect	Δscore
7:55951929:AG:A	donor_loss	1.0000
7:55951932:T:A	donor_loss	1.0000
7:55953173:TTTCA:T	acceptor_loss	1.0000
7:55953174:TTCA:T	acceptor_loss	1.0000
7:55953174:TTCAG:T	acceptor_loss	1.0000
7:55953175:TCAGG:T	acceptor_loss	1.0000
7:55953176:CAGGT:C	acceptor_loss	1.0000
7:55953177:AGGTG:A	acceptor_loss	1.0000
7:55953178:G:T	acceptor_loss	1.0000
7:55953315:AAAG:A	donor_loss	1.0000
7:55953316:AAGG:A	donor_loss	1.0000
7:55953319:GT:G	donor_loss	1.0000
7:55953320:T:G	donor_loss	1.0000
7:55954905:ACAG:A	acceptor_loss	1.0000
7:55954906:CAGT:C	acceptor_loss	1.0000
7:55954907:A:AG	acceptor_gain	1.0000
7:55954907:A:T	acceptor_loss	1.0000
7:55954908:G:GC	acceptor_gain	1.0000
7:55954908:G:GG	acceptor_gain	1.0000
7:55954908:G:T	acceptor_loss	1.0000
7:55954908:GT:G	acceptor_gain	1.0000
7:55954908:GTA:G	acceptor_gain	1.0000
7:55954908:GTATT:G	acceptor_gain	1.0000
7:55951927:GGAG:G	donor_gain	0.9900
7:55951928:GAG:G	donor_gain	0.9900
7:55951928:GAGG:G	donor_gain	0.9900
7:55951931:G:GG	donor_gain	0.9900
7:55951933:GAG:G	donor_loss	0.9900
7:55953177:A:AG	acceptor_gain	0.9900
7:55953177:AGGT:A	acceptor_gain	0.9900

AlphaMissense

826 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
7:55954937:C:A	A51D	0.996
7:55953238:G:T	G15W	0.994
7:55953272:C:A	A26D	0.994
7:55954970:A:T	D62V	0.994
7:55954967:G:T	G61V	0.993
7:55954976:T:A	V64E	0.993
7:55954982:T:A	L66H	0.993
7:55953238:G:A	G15R	0.991
7:55953238:G:C	G15R	0.991
7:55953239:G:A	G15E	0.991
7:55954970:A:C	D62A	0.991
7:55954979:T:C	L65P	0.990
7:55954969:G:C	D62H	0.988
7:55954970:A:G	D62G	0.988
7:55953239:G:T	G15V	0.987
7:55955039:T:A	V85D	0.986
7:55954982:T:C	L66P	0.984
7:55953271:G:C	A26P	0.983
7:55953278:T:A	V28E	0.983
7:55954959:C:G	C58W	0.982
7:55955051:G:T	G89V	0.980
7:55953244:G:A	V17M	0.979
7:55954957:T:C	C58R	0.979
7:55954982:T:G	L66R	0.979
7:55953245:T:A	V17E	0.977
7:55955021:A:C	H79P	0.976
7:55954909:T:G	Y42D	0.975
7:55954969:G:T	D62Y	0.975
7:55955020:C:G	H79D	0.975
7:55954966:G:A	G61R	0.974

dbSNP variants (sampled 300 via entrez): RS1000605941 (7:55956426 A>G), RS1000720848 (7:55956381 A>C), RS1001936043 (7:55951714 C>G), RS1002310820 (7:55951433 C>T), RS1002833693 (7:55954098 A>G), RS1003976141 (7:55951866 G>A), RS1004264508 (7:55951748 G>C,T), RS1004349149 (7:55950979 G>A,T), RS1004634846 (7:55955578 C>T), RS1004673128 (7:55952024 T>A), RS1005385890 (7:55950230 G>A,T), RS1006058414 (7:55952978 G>A), RS1006308926 (7:55956806 C>T), RS1006673906 (7:55954496 G>A,T), RS1006788173 (7:55953587 C>T)

Disease associations

OMIM: gene MIM:611980 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST007382_28	Plasma free amino acid levels (adjusted for twenty other PFAAs)	4.000000e-20
GCST007385_22	Plasma free amino acid levels	2.000000e-16
GCST012251_16	Macular telangiectasia type 2	3.000000e-07
GCST012252_7	Macular telangiectasia type 2	6.000000e-09

EFO canonical traits (3, from GWAS)

EFO ID	Trait name
EFO:0005134	amino acid measurement
EFO:0009774	serine measurement
EFO:1002009	macular telangiectasia type 2

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067387 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

4 potent at pChembl≥5 of 4 total, top 4 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.54	Kd	28.48	nM	CHEMBL5653589
7.52	ED50	30.46	nM	CHEMBL5653589
6.41	Kd	393.3	nM	CHEMBL3752910
6.38	ED50	420.7	nM	CHEMBL3752910

PubChem BioAssay actives

2 with measured affinity, of 4 total; 2 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148774: Binding affinity to human MRPS17 incubated for 45 mins by Kinobead based pull down assay	kd	0.0285	uM
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148774: Binding affinity to human MRPS17 incubated for 45 mins by Kinobead based pull down assay	kd	0.3933	uM

CTD chemical–gene interactions

44 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol F	affects cotreatment, increases expression	2
bisphenol A	decreases expression	2
Leflunomide	decreases expression	2
Particulate Matter	decreases expression, increases abundance, affects cotreatment	2
dicrotophos	decreases expression	1
triphenyl phosphate	affects expression	1
glycidyl methacrylate	increases expression	1
tris(2-butoxyethyl) phosphate	affects expression	1
arsenite	affects binding, decreases reaction	1
sodium arsenite	decreases expression	1
beta-methylcholine	affects expression	1
di-n-butylphosphoric acid	affects expression	1
azoxystrobin	increases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
pyrimidifen	increases expression	1
bisphenol B	increases expression	1
LDN 193189	affects cotreatment, decreases expression	1
picoxystrobin	increases expression	1
(+)-JQ1 compound	increases expression	1
Temozolomide	increases expression	1
Air Pollutants	decreases expression, increases abundance	1
Arsenic	affects methylation	1
Atrazine	decreases expression	1
Caffeine	decreases phosphorylation	1
Dexamethasone	affects cotreatment, increases expression	1
Gasoline	decreases expression, increases abundance, affects cotreatment	1
Hydrogen Peroxide	affects expression	1
Indomethacin	increases expression, affects cotreatment	1
Ivermectin	decreases expression	1
Polycyclic Aromatic Hydrocarbons	increases abundance, affects cotreatment, decreases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5651816	Binding	Binding affinity to human MRPS17 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.