MYCBP

Summary

MYCBP (MYC binding protein, HGNC:7554) is a protein-coding gene on chromosome 1p34.3, encoding c-Myc-binding protein (Q99417). May control the transcriptional activity of MYC.

The protein encoded by this gene binds to the N-terminus of the oncogenic protein C-MYC, enhancing the ability of C-MYC to activate E box-dependent transcription. The encoded protein is normally found in the cytoplasm, but it translocates to the nucleus during S phase of the cell cycle and associates with C-MYC. This protein may be involved in spermatogenesis. This gene can be silenced by microRNA-22. Two transcript variants, one protein-coding and the other probably not protein-coding, have been found for this gene.

Source: NCBI Gene 26292 — RefSeq curated summary.

At a glance

• GWAS associations: 2
• Druggable target: yes
• MANE Select transcript: NM_012333

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:7554
Approved symbol	MYCBP
Name	MYC binding protein
Location	1p34.3
Locus type	gene with protein product
Status	Approved
Aliases	AMY-1
Ensembl gene	ENSG00000214114
Ensembl biotype	protein_coding
OMIM	606535
Entrez	26292

Gene structure

Transcript identifiers

Ensembl transcripts: 7 — 5 protein_coding, 2 protein_coding_CDS_not_defined

ENST00000397572, ENST00000465771, ENST00000494695, ENST00000495043, ENST00000922069, ENST00000922070, ENST00000922071

RefSeq mRNA: 1 — MANE Select: NM_012333 NM_012333

CCDS: CCDS431

Canonical transcript exons

ENST00000397572 — 5 exons

Exon	Start	End
ENSE00001459249	38862493	38864714
ENSE00001926647	38873291	38873348
ENSE00003502757	38867562	38867610
ENSE00003529436	38873018	38873090
ENSE00003611018	38866880	38867009

Expression profiles

Bgee: expression breadth ubiquitous, 224 present calls, max score 94.71.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 25.5526 / max 642.6341, expressed in 1809 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
11844	25.5526	1809

Top tissues by expression

273 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
olfactory segment of nasal mucosa	UBERON:0005386	94.71	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	94.68	gold quality
monocyte	CL:0000576	91.98	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	91.88	gold quality
mononuclear cell	CL:0000842	91.85	gold quality
right testis	UBERON:0004534	91.83	gold quality
mucosa of transverse colon	UBERON:0004991	91.83	gold quality
leukocyte	CL:0000738	91.75	gold quality
left testis	UBERON:0004533	91.48	gold quality
rectum	UBERON:0001052	90.78	gold quality
testis	UBERON:0000473	90.09	gold quality
lower esophagus mucosa	UBERON:0035834	89.48	gold quality
esophagus mucosa	UBERON:0002469	89.47	gold quality
right uterine tube	UBERON:0001302	88.92	gold quality
adrenal tissue	UBERON:0018303	88.66	gold quality
granulocyte	CL:0000094	88.20	gold quality
body of pancreas	UBERON:0001150	88.17	gold quality
islet of Langerhans	UBERON:0000006	88.04	gold quality
esophagus	UBERON:0001043	87.70	gold quality
pancreas	UBERON:0001264	87.10	gold quality
transverse colon	UBERON:0001157	86.57	gold quality
right adrenal gland	UBERON:0001233	86.36	gold quality
lower esophagus	UBERON:0013473	85.91	gold quality
lower esophagus muscularis layer	UBERON:0035833	85.89	gold quality
right adrenal gland cortex	UBERON:0035827	85.75	gold quality
left adrenal gland	UBERON:0001234	85.69	gold quality
body of stomach	UBERON:0001161	85.68	gold quality
epithelium of bronchus	UBERON:0002031	85.38	gold quality
minor salivary gland	UBERON:0001830	85.20	gold quality
oocyte	CL:0000023	85.19	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-GEOD-134144	yes	28.06
E-ANND-3	yes	9.13
E-GEOD-100618	no	357.24

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): CTNNB1, LEF1, MYC

miRNA regulators (miRDB)

124 targeting MYCBP, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-574-5P	100.00	66.01	989
HSA-MIR-4713-3P	100.00	65.92	505
HSA-MIR-4510	100.00	66.60	2050
HSA-MIR-6127	100.00	66.76	2188
HSA-MIR-6129	100.00	66.46	2080
HSA-MIR-6130	100.00	66.69	2012
HSA-MIR-6133	100.00	66.48	2064
HSA-MIR-7110-3P	100.00	73.18	2486
HSA-MIR-8485	100.00	77.57	4731
HSA-MIR-513A-5P	100.00	69.77	2465
HSA-MIR-4500	99.99	72.72	2367
HSA-MIR-511-3P	99.99	68.85	1467
HSA-MIR-3662	99.99	73.82	5684
HSA-LET-7A-5P	99.98	72.29	1790
HSA-LET-7B-5P	99.98	72.31	1790
HSA-LET-7C-5P	99.98	72.29	1790
HSA-LET-7E-5P	99.98	72.29	1790
HSA-LET-7F-5P	99.98	72.56	1784
HSA-LET-7G-5P	99.98	72.37	1784
HSA-LET-7I-5P	99.98	72.37	1788
HSA-MIR-98-5P	99.98	72.33	1787
HSA-LET-7F-2-3P	99.98	70.98	2588
HSA-MIR-1185-1-3P	99.98	71.04	2593
HSA-MIR-1185-2-3P	99.98	71.04	2593
HSA-MIR-499A-5P	99.98	70.79	1323
HSA-MIR-302C-5P	99.97	72.56	3642
HSA-MIR-548AA	99.96	70.64	3753
HSA-MIR-548AP-3P	99.96	70.64	3753
HSA-MIR-548T-3P	99.96	70.64	3753
HSA-MIR-5688	99.96	73.23	4504

Literature-anchored findings (GeneRIF, showing 16)

• AMY-1 was localized in the mitochondria of HeLa and sperm in association with AKAP149 and S-AKAP84, respectively. These results suggest that AMY-1 plays a role in spermatogenesis (PMID:11483602)
• identified a novel AMY-1-binding protein, AAT-1, and determied its expression profiles, genes, and cellular localization; results showed that AAT-1 was specifically expressed in the testis throughout spermatogenesis and was also present in mature sperm (PMID:12223483)
• interacts with S-AKAP84 and AKAP95 in the cytoplasm and the nucleus, respectively, and inhibits enzyme acdtivity by preventing binding of its catalytic subunit to A-kinase-anchoring protein (AKAP) complex (PMID:12414807)
• AMY-1 coordinates ADP-Ribosylation Factors-mediated membrane trafficking and signaling pathways. (PMID:16866877)
• An important role for MBP-1 in the altered cell proliferation and energy utilization that occur in response to an altered glucose concentration. (PMID:17595061)
• Suppression of telomerase activity mediated by PinX1 is involved in the Mad1/c-Myc pathway. (PMID:20544396)
• miR-22 acts as a tumor suppressor through direct repression of MYCBP expression and subsequent reduction of oncogenic c-Myc activities. (PMID:20562918)
• c-Myc expression is reduced in pterygium compared with normal conjunctiva. (PMID:22050566)
• Weighted gene co-expression network analysis identifies specific modules and hub genes related to subsyndromal symptomatic depression. (PMID:30489189)
• lnc-XLEC1 had inhibitive effects that may result from its collaboration with MBP-1 (MYCBP). (PMID:30698832)
• Through activating MYCBP and by sponging miR-574-5p. (PMID:31094023)
• Data found that MYCBP was highly expressed in hepatocellular carcinoma (HCC) tissues. Its overexpression is correlated with poor prognosis which indicates that MYCBP might play a cancer-promoting role in HCC. Further results found that EYA4 suppressed HCC tumor cell growth by repressing MYCBP by dephosphorylating beta-catenin S552. (PMID:31385398)
• lncRNA LUNAR1 accelerates colorectal cancer progression by targeting the miR4953p/MYCBP axis. (PMID:33300052)
• TAZ is indispensable for c-MYC-induced hepatocarcinogenesis. (PMID:34464659)
• MiR-26b-5p inhibits cell proliferation and EMT by targeting MYCBP in triple-negative breast cancer. (PMID:34895159)
• Circ_0002669 promotes osteosarcoma tumorigenesis through directly binding to MYCBP and sponging miR-889-3p. (PMID:38570856)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	mycbp	ENSDARG00000091001
mus_musculus	Mycbp	ENSMUSG00000028647
rattus_norvegicus	Mycbp	ENSRNOG00000065724

Protein

Protein identifiers

c-Myc-binding protein — Q99417 (reviewed: Q99417)

Alternative names: Associate of Myc 1

All UniProt accessions (2): A0A0D9SEI7, Q99417

UniProt curated annotations — full annotation on UniProt →

Function. May control the transcriptional activity of MYC. Stimulates the activation of E box-dependent transcription by MYC.

Subunit / interactions. Binds via its C-terminal region to the N-terminal region of MYC. Associates with AKAP1/S-AKAP84. Interacts with MYCBPAP. Interacts with CFAP91. Interacts with CFAP65 in a MYCBPAP-dependent manner. Interacts with OTUD4.

Subcellular location. Cytoplasm. Nucleus. Mitochondrion.

Tissue specificity. Highly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung.

Similarity. Belongs to the AMY1 family.

RefSeq proteins (1): NP_036465* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR026060	AMY1	Family

UniProt features (7 total): helix 2, initiator methionine 1, chain 1, sequence conflict 1, strand 1, turn 1

Structure

Experimental structures (PDB)

1 structures.

PDB	Method	Resolution (Å)
2YY0	X-RAY DIFFRACTION	2.4

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q99417-F1	86.24	0.66

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 174 (showing top): BUYTAERT_PHOTODYNAMIC_THERAPY_STRESS_DN, TGCGCANK_UNKNOWN, GCANCTGNY_MYOD_Q6, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_UP, GRAESSMANN_RESPONSE_TO_MC_AND_DOXORUBICIN_UP, IVANOVA_HEMATOPOIESIS_LATE_PROGENITOR, THEILGAARD_NEUTROPHIL_AT_SKIN_WOUND_DN, GOBP_MALE_GAMETE_GENERATION, MODULE_16, CTATGCA_MIR153, CAGCTG_AP4_Q5, MILI_PSEUDOPODIA_HAPTOTAXIS_UP, CCTGTGA_MIR513, chr1p34, AAAGACA_MIR511

GO Biological Process (3): regulation of DNA-templated transcription (GO:0006355), spermatogenesis (GO:0007283), positive regulation of DNA-templated transcription (GO:0045893)

GO Molecular Function (2): transcription coactivator activity (GO:0003713), protein binding (GO:0005515)

GO Cellular Component (4): nucleus (GO:0005634), nucleoplasm (GO:0005654), cytoplasm (GO:0005737), mitochondrion (GO:0005739)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
DNA-templated transcription	2
intracellular membrane-bounded organelle	2
cellular anatomical structure	2
regulation of gene expression	1
regulation of RNA biosynthetic process	1
developmental process involved in reproduction	1
male gamete generation	1
regulation of DNA-templated transcription	1
positive regulation of RNA biosynthetic process	1
transcription coregulator activity	1
positive regulation of DNA-templated transcription	1
binding	1
nuclear lumen	1
intracellular anatomical structure	1
cytoplasm	1

Protein interactions and networks

STRING

1289 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
MYCBP	MYCBPAP	Q8TBZ2	942
MYCBP	MYC	P01106	918
MYCBP	CFAP91	Q7Z4T9	915
MYCBP	AKAP1	Q92667	891
MYCBP	ENO1	P06733	506
MYCBP	CRELD1	Q96HD1	442
MYCBP	PLA2G4A	P47712	439
MYCBP	GIPC2	Q8TF65	436
MYCBP	PRKAR2B	P31323	424
MYCBP	MYCBP2	O75592	410
MYCBP	ESR1	P03372	406
MYCBP	GADD45G	O95257	404
MYCBP	PTN	P21246	401
MYCBP	POLR3E	Q9NVU0	396
MYCBP	MXI1	P50539	392

IntAct

103 interactions, top by confidence:

A	B	Type	Score
YWHAB	PIK3C2A	psi-mi:“MI:0914”(association)	0.800
ARFGEF2	MYCBP	psi-mi:“MI:0915”(physical association)	0.790
MYCBP	ARFGEF1	psi-mi:“MI:0915”(physical association)	0.790
MYCBP	ARFGEF2	psi-mi:“MI:0915”(physical association)	0.790
ARFGEF1	MYCBP	psi-mi:“MI:0915”(physical association)	0.790
MYCBP	ARFGEF2	psi-mi:“MI:0403”(colocalization)	0.790
ARFGEF1	MYCBP	psi-mi:“MI:0403”(colocalization)	0.790
HEXIM2	AHCYL1	psi-mi:“MI:0914”(association)	0.740
DYNLL1	BLTP3B	psi-mi:“MI:0914”(association)	0.730
KDM5A	SIN3B	psi-mi:“MI:0914”(association)	0.640
YWHAG	BLTP3B	psi-mi:“MI:0914”(association)	0.640
DYNLL2	BLTP3B	psi-mi:“MI:0914”(association)	0.640
MYCBP	MYCBPAP	psi-mi:“MI:0915”(physical association)	0.620
MYCBPAP	MYCBP	psi-mi:“MI:0915”(physical association)	0.620
CFAP91	MYCBP	psi-mi:“MI:0915”(physical association)	0.610
MYCBP	CFAP91	psi-mi:“MI:0407”(direct interaction)	0.610
CFAP91	MYCBP	psi-mi:“MI:0403”(colocalization)	0.610
MYCBP	CFAP91	psi-mi:“MI:0403”(colocalization)	0.610
YWHAE	PIK3C2A	psi-mi:“MI:0914”(association)	0.570
YWHAH	BLTP3B	psi-mi:“MI:0914”(association)	0.570
YWHAZ	PIK3C2A	psi-mi:“MI:0914”(association)	0.570
AKAP8	MYCBP	psi-mi:“MI:0915”(physical association)	0.550
MYCBP	AKAP8	psi-mi:“MI:0914”(association)	0.550

BioGRID (138): MYCBP (Affinity Capture-MS), EIF5AL1 (Co-fractionation), MYCBP (Co-fractionation), MYCBP (Co-fractionation), MYCBP (Co-fractionation), MYCBP (Co-fractionation), TPT1 (Co-fractionation), MYCBP (Affinity Capture-MS), MYCBP (Affinity Capture-MS), MYCBP (Affinity Capture-MS), MYCBP (Reconstituted Complex), MYCBP (Affinity Capture-MS), MYCBP (Affinity Capture-MS), MYCBP (Affinity Capture-MS), MYCBP (Affinity Capture-MS)

ESM2 similar proteins: A2XK00, B2RXB2, B5X3I1, C0HMA2, D4A9E1, G0RY74, G0RYE9, O14348, O17453, O75506, O95295, P60192, Q0V9C8, Q0VCF3, Q13503, Q16RX1, Q178E6, Q17NH9, Q2TBP7, Q2TBU8, Q3ZC22, Q4R6N3, Q5R561, Q5R7A8, Q5R8J5, Q5RDI2, Q5RE46, Q5RGJ6, Q5XJ97, Q5ZKJ4, Q68EW7, Q6ASS9, Q6DDT0, Q6DF11, Q6P255, Q7QB45, Q7T338, Q7ZTI5, Q8GW48, Q8K3X8

Diamond homologs: Q2TBP7, Q54I57, Q5R7A8, Q99417, Q9EQS3, Q9VG76

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 111 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Activation of BAD and translocation to mitochondria	5	52.1×	5e-06
Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex	5	46.0×	6e-06
SARS-CoV-1 targets host intracellular signalling and regulatory pathways	5	46.0×	6e-06
Activation of BH3-only proteins	5	34.0×	2e-05
RHO GTPases activate PKNs	5	21.7×	1e-04
Intrinsic Pathway for Apoptosis	5	20.1×	1e-04
Apoptosis	6	13.8×	1e-04
Translocation of SLC2A4 (GLUT4) to the plasma membrane	6	12.7×	2e-04

Disease & clinical

Clinical variants and AI predictions

ClinVar

0 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	0
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

919 predictions. Top by Δscore:

Variant	Effect	Δscore
1:38867534:AAAG:A	donor_gain	1.0000
1:38873016:AC:A	donor_gain	1.0000
1:38873017:CC:C	donor_gain	1.0000
1:38873289:A:AC	donor_gain	1.0000
1:38873290:C:CC	donor_gain	1.0000
1:38864711:CAAG:C	acceptor_gain	0.9900
1:38866875:TTTA:T	donor_loss	0.9900
1:38866876:TTA:T	donor_loss	0.9900
1:38866877:TA:T	donor_loss	0.9900
1:38866878:AC:A	donor_loss	0.9900
1:38866879:CCTT:C	donor_loss	0.9900
1:38873327:G:C	donor_gain	0.9900
1:38864715:C:CC	acceptor_gain	0.9800
1:38867547:G:T	donor_gain	0.9800
1:38873013:CTCA:C	donor_loss	0.9800
1:38873014:TCA:T	donor_loss	0.9800
1:38873015:CA:C	donor_loss	0.9800
1:38873016:A:AC	donor_gain	0.9800
1:38873016:A:AG	donor_loss	0.9800
1:38873017:C:CC	donor_gain	0.9800
1:38873285:CCTCA:C	donor_loss	0.9800
1:38873286:CTCA:C	donor_loss	0.9800
1:38873287:TCA:T	donor_loss	0.9800
1:38873288:CACTT:C	donor_loss	0.9800
1:38873289:ACTTT:A	donor_loss	0.9800
1:38864714:GC:G	acceptor_loss	0.9700
1:38864715:C:CA	acceptor_loss	0.9700
1:38866877:TAC:T	donor_loss	0.9700
1:38866879:CCTTT:C	donor_loss	0.9700
1:38873012:GCTCA:G	donor_loss	0.9700

AlphaMissense

663 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:38867006:A:C	F47L	1.000
1:38867006:A:T	F47L	1.000
1:38867008:A:G	F47L	1.000
1:38873053:A:G	L18S	1.000
1:38873064:G:C	F14L	1.000
1:38873064:G:T	F14L	1.000
1:38873065:A:G	F14S	1.000
1:38873066:A:G	F14L	1.000
1:38867577:G:T	P41H	0.999
1:38867578:G:A	P41S	0.999
1:38867598:A:G	L34S	0.999
1:38867607:A:G	L31S	0.999
1:38873042:C:A	G22W	0.999
1:38873053:A:C	L18W	0.999
1:38873062:C:G	R15P	0.999
1:38873065:A:C	F14C	0.999
1:38873075:G:A	R11C	0.999
1:38873075:G:T	R11S	0.999
1:38867568:G:T	A44D	0.998
1:38867577:G:A	P41L	0.998
1:38867579:T:A	K40N	0.998
1:38867579:T:G	K40N	0.998
1:38867596:A:G	Y35H	0.998
1:38873023:G:A	T28I	0.998
1:38873026:A:G	L27P	0.998
1:38873041:C:A	G22V	0.998
1:38873041:C:T	G22E	0.998
1:38873051:C:T	E19K	0.998
1:38873052:C:A	L18F	0.998
1:38873052:C:G	L18F	0.998

dbSNP variants (sampled 300 via entrez): RS1000003932 (1:38867170 G>A), RS1000060196 (1:38864286 G>C), RS1000657994 (1:38862595 A>G), RS1001491394 (1:38863936 G>C,T), RS1001546920 (1:38866694 C>T), RS1001744444 (1:38871428 T>C), RS1002084368 (1:38874208 A>G), RS1002429876 (1:38868533 A>G), RS1002481204 (1:38862112 T>G), RS1002491833 (1:38872852 G>A), RS1002649774 (1:38865083 G>T), RS1003087400 (1:38873166 C>A,G,T), RS1003403133 (1:38867339 G>C), RS1003488645 (1:38871076 C>T), RS1003684903 (1:38870078 G>A)

Disease associations

OMIM: gene MIM:606535 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

Study	Trait	p-value
GCST003475_2	Beard thickness	8.000000e-06
GCST005312_27	Menopause (age at onset)	5.000000e-21

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0004704	age at menopause

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067196 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

46 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
methylmercuric chloride	decreases expression	3
bisphenol A	decreases expression, increases expression	3
Air Pollutants	affects cotreatment, increases abundance, increases expression, affects expression	3
sodium arsenite	decreases expression, increases expression	2
Cisplatin	decreases expression, decreases reaction, increases expression	2
Ozone	affects cotreatment, increases expression, increases abundance, affects expression	2
Cyclosporine	decreases expression	2
triphenyl phosphate	affects expression	1
alpha-pinene	affects cotreatment, increases expression, increases abundance	1
methylparaben	decreases expression	1
tris(1,3-dichloro-2-propyl)phosphate	decreases expression	1
cobaltous chloride	decreases expression	1
2,3-bis(3’-hydroxybenzyl)butyrolactone	affects cotreatment, increases expression	1
methacrylaldehyde	increases expression, increases abundance, affects cotreatment	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
abrine	decreases expression	1
jinfukang	decreases expression	1
LDN 193189	affects cotreatment, decreases expression	1
NSC 689534	affects binding, decreases expression	1
Acrolein	affects cotreatment, increases expression, increases abundance	1
Benzo(a)pyrene	increases methylation	1
Copper	affects binding, decreases expression	1
Coumestrol	affects cotreatment, increases expression	1
Doxorubicin	affects response to substance	1
Estradiol	increases expression	1
Formaldehyde	decreases expression	1
Methyl Methanesulfonate	decreases expression	1
Phenobarbital	affects expression	1
Piroxicam	decreases expression, decreases reaction	1
Silicon Dioxide	decreases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652935	Binding	Binding affinity to human MYCBP incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.