NDUFB1

Summary

NDUFB1 (NADH:ubiquinone oxidoreductase subunit B1, HGNC:7695) is a protein-coding gene on chromosome 14q31.3, encoding NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 (O75438). Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed not to be involved in catalysis. It is a selective cancer dependency (DepMap: 73.3% of cell lines).

Predicted to enable NADH dehydrogenase (ubiquinone) activity. Predicted to be involved in mitochondrial electron transport, NADH to ubiquinone and proton motive force-driven mitochondrial ATP synthesis. Located in mitochondrial inner membrane and nuclear speck. Part of respiratory chain complex I.

Source: NCBI Gene 4707 — RefSeq curated summary.

At a glance

• GWAS associations: 3
• Clinical variants (ClinVar): 12 total
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 73.3% of screened cell lines
• MANE Select transcript: NM_004545

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:7695
Approved symbol	NDUFB1
Name	NADH:ubiquinone oxidoreductase subunit B1
Location	14q31.3
Locus type	gene with protein product
Status	Approved
Aliases	MNLL, CI-MNLL
Ensembl gene	ENSG00000183648
Ensembl biotype	protein_coding
OMIM	603837
Entrez	4707

Gene structure

Transcript identifiers

Ensembl transcripts: 31 — 29 protein_coding, 1 retained_intron, 1 protein_coding_CDS_not_defined

ENST00000329559, ENST00000553514, ENST00000553666, ENST00000555441, ENST00000556555, ENST00000605997, ENST00000617122, ENST00000909059, ENST00000909060, ENST00000909061, ENST00000909062, ENST00000909063, ENST00000909064, ENST00000909065, ENST00000909066, ENST00000932813, ENST00000932814, ENST00000932815, ENST00000932816, ENST00000932817, ENST00000932818, ENST00000932819, ENST00000932820, ENST00000932821, ENST00000932822, ENST00000932823, ENST00000932824, ENST00000932825, ENST00000949683, ENST00000949684, ENST00000949685

RefSeq mRNA: 1 — MANE Select: NM_004545 NM_004545

CCDS: CCDS9901

Canonical transcript exons

ENST00000605997 — 3 exons

Exon	Start	End
ENSE00002475112	92116123	92116229
ENSE00003695332	92117498	92117642
ENSE00003697397	92121642	92121706

Expression profiles

Bgee: expression breadth ubiquitous, 288 present calls, max score 99.63.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 62.2639 / max 396.8956, expressed in 1822 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
144578	61.0300	1822
144579	1.2339	644

Top tissues by expression

288 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
cardiac ventricle	UBERON:0002082	99.63	gold quality
heart left ventricle	UBERON:0002084	99.63	gold quality
apex of heart	UBERON:0002098	99.63	gold quality
right atrium auricular region	UBERON:0006631	99.60	gold quality
cardiac atrium	UBERON:0002081	99.57	gold quality
heart right ventricle	UBERON:0002080	99.53	gold quality
heart	UBERON:0000948	99.51	gold quality
renal medulla	UBERON:0000362	99.46	gold quality
adenohypophysis	UBERON:0002196	99.40	gold quality
endothelial cell	CL:0000115	99.39	gold quality
left adrenal gland cortex	UBERON:0035825	99.38	gold quality
left adrenal gland	UBERON:0001234	99.36	gold quality
hindlimb stylopod muscle	UBERON:0004252	99.36	gold quality
mucosa of transverse colon	UBERON:0004991	99.36	gold quality
body of tongue	UBERON:0011876	99.36	gold quality
pituitary gland	UBERON:0000007	99.35	gold quality
nucleus accumbens	UBERON:0001882	99.35	gold quality
adrenal cortex	UBERON:0001235	99.34	gold quality
biceps brachii	UBERON:0001507	99.34	gold quality
caudate nucleus	UBERON:0001873	99.34	gold quality
calcaneal tendon	UBERON:0003701	99.34	gold quality
right adrenal gland	UBERON:0001233	99.33	gold quality
muscle of leg	UBERON:0001383	99.32	gold quality
amygdala	UBERON:0001876	99.32	gold quality
myocardium	UBERON:0002349	99.32	gold quality
gastrocnemius	UBERON:0001388	99.31	gold quality
left ventricle myocardium	UBERON:0006566	99.31	gold quality
cingulate cortex	UBERON:0003027	99.29	gold quality
anterior cingulate cortex	UBERON:0009835	99.29	gold quality
right adrenal gland cortex	UBERON:0035827	99.29	gold quality

Single-cell (SCXA)

Detected in 13 experiment(s), a significant marker in 6.

Experiment	Marker?	Max mean expression
E-GEOD-89232	yes	1995.18
E-ANND-3	yes	18.08
E-HCAD-10	yes	11.96
E-GEOD-135922	yes	8.23
E-MTAB-6678	yes	8.21
E-MTAB-10042	yes	7.26
E-CURD-79	no	378.59
E-MTAB-6075	no	246.80
E-MTAB-7606	no	165.38
E-GEOD-125970	no	14.88
E-MTAB-9388	no	13.56
E-CURD-112	no	8.82
E-GEOD-81547	no	4.43

Regulation

Is transcription factor: no

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 73.3% of screened cell lines.

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	ndufb1	ENSDARG00000087456
mus_musculus	Ndufb1	ENSMUSG00000113902
rattus_norvegicus	Ndufb1	ENSRNOG00000088065

Protein

Protein identifiers

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 — O75438 (reviewed: O75438)

Alternative names: Complex I-MNLL, NADH-ubiquinone oxidoreductase MNLL subunit

All UniProt accessions (1): O75438

UniProt curated annotations — full annotation on UniProt →

Function. Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subunit / interactions. Complex I is composed of 45 different subunits.

Subcellular location. Mitochondrion inner membrane.

Similarity. Belongs to the complex I NDUFB1 subunit family.

Isoforms (2)

UniProt ID	Names	Canonical?
O75438-1	1	yes
O75438-2	2

RefSeq proteins (1): NP_004536* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR012575	NDUB1	Family

Pfam: PF08040

UniProt features (4 total): initiator methionine 1, chain 1, transmembrane region 1, splice variant 1

Structure

Experimental structures (PDB)

7 structures.

PDB	Method	Resolution (Å)
9I4I	ELECTRON MICROSCOPY	2.63
9TI4	ELECTRON MICROSCOPY	2.66
9CWT	ELECTRON MICROSCOPY	3.44
5XTC	ELECTRON MICROSCOPY	3.7
5XTD	ELECTRON MICROSCOPY	3.7
5XTH	ELECTRON MICROSCOPY	3.9
5XTI	ELECTRON MICROSCOPY	17.4

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-O75438-F1	91.24	0.73

Function

Pathways and Gene Ontology

Reactome pathways

4 pathways

ID	Pathway
R-HSA-611105	Respiratory electron transport
R-HSA-6799198	Complex I biogenesis
R-HSA-1428517	Aerobic respiration and respiratory electron transport
R-HSA-1430728	Metabolism

MSigDB gene sets: 163 (showing top): MODULE_93, GGGNRMNNYCAT_UNKNOWN, MODULE_151, MODULE_77, GOBP_ORGANOPHOSPHATE_METABOLIC_PROCESS, GOBP_NUCLEOSIDE_PHOSPHATE_BIOSYNTHETIC_PROCESS, GOBP_ORGANOPHOSPHATE_BIOSYNTHETIC_PROCESS, GOBP_CARBOHYDRATE_DERIVATIVE_METABOLIC_PROCESS, GOBP_MONOATOMIC_CATION_TRANSPORT, GOBP_NUCLEOBASE_CONTAINING_SMALL_MOLECULE_METABOLIC_PROCESS, GOBP_GENERATION_OF_PRECURSOR_METABOLITES_AND_ENERGY, GOBP_ATP_BIOSYNTHETIC_PROCESS, GOBP_OXIDATIVE_PHOSPHORYLATION, GOBP_CARBOHYDRATE_DERIVATIVE_BIOSYNTHETIC_PROCESS, GOBP_NUCLEOSIDE_TRIPHOSPHATE_METABOLIC_PROCESS

GO Biological Process (5): mitochondrial electron transport, NADH to ubiquinone (GO:0006120), aerobic respiration (GO:0009060), proton motive force-driven mitochondrial ATP synthesis (GO:0042776), SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition (GO:0006617), proton transmembrane transport (GO:1902600)

GO Molecular Function (1): NADH dehydrogenase (ubiquinone) activity (GO:0008137)

GO Cellular Component (6): mitochondrion (GO:0005739), mitochondrial inner membrane (GO:0005743), nuclear speck (GO:0016607), respiratory chain complex I (GO:0045271), signal recognition particle, endoplasmic reticulum targeting (GO:0005786), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-3 pathways:

Category	Pathways
Aerobic respiration and respiratory electron transport	1
Respiratory electron transport	1
Metabolism	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
aerobic electron transport chain	1
mitochondrial ATP synthesis coupled electron transport	1
cellular respiration	1
mitochondrion	1
oxidative phosphorylation	1
proton motive force-driven ATP synthesis	1
SRP-dependent cotranslational protein targeting to membrane	1
protein-containing complex assembly	1
monoatomic cation transmembrane transport	1
NADH dehydrogenase activity	1
electron transfer activity	1
proton transmembrane transporter activity	1
oxidoreduction-driven active transmembrane transporter activity	1
oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor	1
active monoatomic ion transmembrane transporter activity	1
cytoplasm	1
intracellular membrane-bounded organelle	1
organelle inner membrane	1
mitochondrial membrane	1
nuclear ribonucleoprotein granule	1
NADH dehydrogenase complex	1
respiratory chain complex	1
transmembrane transporter complex	1
signal recognition particle	1
cellular anatomical structure	1

Protein interactions and networks

STRING

1104 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
NDUFB1	NDUFA2	O43678	920
NDUFB1	NDUFB5	O43674	897
NDUFB1	NDUFB11	Q9NX14	896
NDUFB1	NDUFB10	O96000	892
NDUFB1	NDUFB6	O95139	874
NDUFB1	NDUFB4	O95168	845
NDUFB1	MT-ND4	P03905	820
NDUFB1	NDUFA1	O15239	788
NDUFB1	COXFA4	O00483	750
NDUFB1	NDUFB9	Q9Y6M9	729
NDUFB1	NDUFA7	O95182	705
NDUFB1	NDUFA11	Q86Y39	691
NDUFB1	NDUFA5	Q16718	689
NDUFB1	NDUFB3	O43676	683
NDUFB1	NDUFS2	O75306	673

IntAct

62 interactions, top by confidence:

A	B	Type	Score
NDUFS3	NDUFS8	psi-mi:“MI:0914”(association)	0.730
NDUFS6	NDUFS8	psi-mi:“MI:0914”(association)	0.640
NDUFB5	NDUFB3	psi-mi:“MI:0914”(association)	0.640
NDUFAF4	NDUFS8	psi-mi:“MI:0914”(association)	0.640
NDUFA13	NDUFS8	psi-mi:“MI:0914”(association)	0.640
NDUFA9	NDUFS4	psi-mi:“MI:0914”(association)	0.640
NDUFA9	NDUFS8	psi-mi:“MI:0914”(association)	0.530
C3AR1	TMEM120B	psi-mi:“MI:0914”(association)	0.530
NDUFA8	NDUFS8	psi-mi:“MI:0914”(association)	0.530
ECSIT	NDUFS8	psi-mi:“MI:0914”(association)	0.530
SPPL2B	UQCRQ	psi-mi:“MI:0914”(association)	0.530
NDUFV2	NDUFS8	psi-mi:“MI:0914”(association)	0.530
NDUFC2	NDUFS4	psi-mi:“MI:0914”(association)	0.530
DNAJC30	NDUFS8	psi-mi:“MI:0914”(association)	0.530
	FER1L5	psi-mi:“MI:0915”(physical association)	0.400
NDUFB1	NDUFS5	psi-mi:“MI:0915”(physical association)	0.400
EWSR1	NDUFB1	psi-mi:“MI:0915”(physical association)	0.370
NDUFA5	NDUFB1	psi-mi:“MI:0915”(physical association)	0.370

BioGRID (93): NDUFB1 (Affinity Capture-MS), NDUFB1 (Affinity Capture-MS), NDUFB1 (Affinity Capture-MS), NDUFB1 (Two-hybrid), NDUFB1 (Two-hybrid), NDUFB1 (Affinity Capture-MS), NDUFB1 (Affinity Capture-MS), NDUFB1 (Affinity Capture-MS), NDUFB1 (Affinity Capture-MS), NDUFB1 (Affinity Capture-MS), NDUFB1 (Affinity Capture-MS), NDUFB1 (Affinity Capture-MS), NDUFB1 (Affinity Capture-MS), NDUFB1 (Affinity Capture-MS), NDUFB1 (Affinity Capture-MS)

ESM2 similar proteins: A0A096LP01, A6ZLK2, A6ZRY0, A7EJE5, A7TMN2, A7TN96, B3LMP9, B3LNV9, B5VQU2, C4XVW1, C5DDY0, C5DGX3, C5DQ18, C5DRK2, C5DRL9, C5E0J8, C5E381, C7GMW8, C7GR78, D3UF10, G2TRM8, O74383, O75438, P0CB73, P0CB74, P0DN34, P0DN35, P26310, P34526, P38341, P46998, P50087, P50945, P53224, Q02378, Q0MQC5, Q0MQC6, Q12082, Q6CLL5, Q6CTE6

Diamond homologs: O75438, P0CB73, P0CB74, P0DN34, P0DN35, Q02378, Q0MQC5, Q0MQC6

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
NDUFB1	“form complex”	“NADH-ubiquinone oxidoreductase-Mitochondrial respiratory chain complex I”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 45 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Complex I biogenesis	22	110.3×	4e-41
Respiratory electron transport	22	63.4×	2e-35
Aerobic respiration and respiratory electron transport	20	53.6×	2e-30

GO biological processes:

GO term	Partners	Fold	FDR
mitochondrial electron transport, NADH to ubiquinone	17	156.3×	3e-33
proton motive force-driven mitochondrial ATP synthesis	19	128.3×	5e-35
aerobic respiration	19	120.7×	1e-34
mitochondrial respiratory chain complex I assembly	10	105.4×	5e-17

Disease & clinical

Clinical variants and AI predictions

ClinVar

12 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	8
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

510 predictions. Top by Δscore:

Variant	Effect	Δscore
14:92117493:CTAA:C	donor_loss	1.0000
14:92117494:TAA:T	donor_loss	1.0000
14:92117496:A:AG	donor_loss	1.0000
14:92117497:C:CA	donor_loss	1.0000
14:92117512:A:AC	donor_gain	1.0000
14:92117513:C:CC	donor_gain	1.0000
14:92117513:CT:C	donor_gain	1.0000
14:92117640:TAG:T	acceptor_gain	1.0000
14:92117643:C:CC	acceptor_gain	1.0000
14:92116226:TTCC:T	acceptor_gain	0.9900
14:92116227:TCC:T	acceptor_gain	0.9900
14:92116228:CC:C	acceptor_gain	0.9900
14:92116228:CCC:C	acceptor_gain	0.9900
14:92116229:CC:C	acceptor_gain	0.9900
14:92116230:C:A	acceptor_loss	0.9900
14:92116242:C:CT	acceptor_gain	0.9900
14:92116243:A:T	acceptor_gain	0.9900
14:92117638:GATAG:G	acceptor_gain	0.9900
14:92117641:AG:A	acceptor_gain	0.9900
14:92117642:GCTAA:G	acceptor_loss	0.9900
14:92117644:T:A	acceptor_loss	0.9900
14:92121779:C:CT	donor_gain	0.9900
14:92121780:T:TT	donor_gain	0.9900
14:92116225:ATTCC:A	acceptor_gain	0.9800
14:92116230:C:CC	acceptor_gain	0.9800
14:92116232:G:C	acceptor_gain	0.9800
14:92116234:G:GC	acceptor_gain	0.9800
14:92117639:ATAG:A	acceptor_gain	0.9800
14:92121694:AAC:A	donor_gain	0.9800
14:92121720:TCGGG:T	donor_gain	0.9800

AlphaMissense

381 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
14:92117524:G:C	F38L	0.816
14:92117524:G:T	F38L	0.816
14:92117526:A:G	F38L	0.816
14:92117575:A:C	F21L	0.808
14:92117575:A:T	F21L	0.808
14:92117577:A:G	F21L	0.808
14:92117512:A:C	S42R	0.766
14:92117512:A:T	S42R	0.766
14:92117514:T:G	S42R	0.766
14:92117503:A:C	F45L	0.761
14:92117503:A:T	F45L	0.761
14:92117505:A:G	F45L	0.761
14:92117573:A:T	V22D	0.739
14:92117580:C:G	G20R	0.691
14:92117580:C:T	G20R	0.691
14:92116229:C:A	R47S	0.679
14:92116229:C:G	R47S	0.679
14:92117588:A:T	V17D	0.621
14:92116196:C:A	K58N	0.578
14:92116196:C:G	K58N	0.578
14:92117567:C:T	G24E	0.571
14:92117507:A:G	L44S	0.567
14:92117602:C:A	W12C	0.566
14:92117602:C:G	W12C	0.566

dbSNP variants (sampled 300 via entrez): RS1000066590 (14:92122001 C>G,T), RS1001472705 (14:92121377 C>G), RS1001524836 (14:92121682 A>C), RS1001573905 (14:92117459 A>G), RS1001686258 (14:92123099 T>C), RS1001735421 (14:92116082 C>T), RS1001809471 (14:92122439 A>C), RS1002083020 (14:92115822 A>C), RS1002784768 (14:92121711 G>A,T), RS1003094409 (14:92122006 G>A,C,T), RS1004237410 (14:92122029 C>G,T), RS1005066000 (14:92117846 A>C,G), RS1005130171 (14:92123359 C>T), RS1005359661 (14:92118123 T>C), RS1005954623 (14:92116800 T>C)

Disease associations

OMIM: gene MIM:603837 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

3 associations (top):

Study	Trait	p-value
GCST006291_57	Spherical equivalent or myopia (age of diagnosis)	7.000000e-11
GCST009614_3	LDL cholesterol levels x loop diuretics use interaction	4.000000e-07
GCST010002_158	Refractive error	4.000000e-24

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0004847	age at onset
EFO:0004611	low density lipoprotein cholesterol measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL2363065 (PROTEIN COMPLEX)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

8 potent at pChembl≥5 of 18 total, top 8 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
6.06	IC50	870	nM	R-(+)-MARMIN-6’-UNDECANOATE
6.04	IC50	920	nM	R-(+)-MARMIN-6’-LINOLEATE
5.63	IC50	2350	nM	R-(+)-MARMIN-6’-LINOLEATE
5.51	IC50	3080	nM	R-(+)-MARMIN-6’-OCTANOATE
5.43	IC50	3670	nM	R-(+)-MARMIN-6’-UNDECANOATE
5.43	IC50	3710	nM	R-(+)-MARMIN-6’-OCTANOATE
5.31	IC50	4900	nM	(+)-9’-ISOVALEROXYLARICIRESINOL
5.04	IC50	9100	nM	(+)-9’-ISOVALEROXYLARICIRESINOL

PubChem BioAssay actives

8 with measured affinity, of 28 total; 4 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
[(E,3R)-2-hydroxy-2,6-dimethyl-8-(2-oxochromen-7-yl)oxyoct-6-en-3-yl] undecanoate	739270: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	ic50	0.8700	uM
[(E,3R)-2-hydroxy-2,6-dimethyl-8-(2-oxochromen-7-yl)oxyoct-6-en-3-yl] (9Z,12Z)-octadeca-9,12-dienoate	739270: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	ic50	0.9200	uM
[(E,3R)-2-hydroxy-2,6-dimethyl-8-(2-oxochromen-7-yl)oxyoct-6-en-3-yl] octanoate	739270: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	ic50	3.0800	uM
[(2S,3R,4R)-2-(4-hydroxy-3-methoxyphenyl)-4-[(4-hydroxy-3-methoxyphenyl)methyl]oxolan-3-yl]methyl 3-methylbutanoate	739269: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1,10-phenanthroline-induced HIF1 activation incubated for 30 mins prior to 1,10-phenanthroline-challenge measured after 16 hrs by luciferase reporter assay	ic50	4.9000	uM

CTD chemical–gene interactions

55 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Acetaminophen	decreases expression, increases expression, affects cotreatment	4
bisphenol A	affects expression, decreases expression, increases expression	3
methylmercuric chloride	increases expression, decreases expression	2
mercuric bromide	affects cotreatment, decreases expression	2
entinostat	decreases expression, affects cotreatment	2
Panobinostat	affects cotreatment, decreases expression	2
Air Pollutants	affects expression, increases abundance, decreases expression	2
Phenylmercuric Acetate	affects cotreatment, decreases expression	2
Cyclosporine	decreases expression	2
p-Chloromercuribenzoic Acid	affects cotreatment, decreases expression	2
aristolochic acid I	decreases expression	1
bisphenol F	affects cotreatment, increases expression	1
dicrotophos	decreases expression	1
triphenyl phosphate	affects expression	1
arsenite	increases reaction, affects binding	1
tris(1,3-dichloro-2-propyl)phosphate	decreases expression	1
sodium arsenite	increases expression	1
cobaltous chloride	decreases expression	1
perfluorooctanoic acid	decreases expression	1
acipimox	decreases expression	1
CGP 52608	affects binding, increases reaction	1
perfluoro-n-nonanoic acid	decreases expression	1
OSW 1	increases expression	1
corosolic acid	decreases expression	1
K 7174	decreases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
dorsomorphin	affects cotreatment, decreases expression	1
Resveratrol	affects cotreatment, increases expression	1
Sunitinib	decreases expression	1
Atrazine	decreases expression	1

ChEMBL screening assays

4 unique, capped per target: 4 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL2353025	Binding	Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation at 30 uM incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	Semisynthetic studies identify mitochondria poisons from botanical dietary supplements–geranyloxycoumarins from Aegle marmelos. — Bioorg Med Chem

Cellosaurus cell lines

1 cell lines: 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B3C4	Abcam HEK293T NDUFB1 KO	Transformed cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.