NDUFB5

Summary

NDUFB5 (NADH:ubiquinone oxidoreductase subunit B5, HGNC:7700) is a protein-coding gene on chromosome 3q26.33, encoding NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (O43674). Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. It is a selective cancer dependency (DepMap: 14.2% of cell lines).

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene.

Source: NCBI Gene 4711 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 51 total
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 14.2% of screened cell lines
• MANE Select transcript: NM_002492

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:7700
Approved symbol	NDUFB5
Name	NADH:ubiquinone oxidoreductase subunit B5
Location	3q26.33
Locus type	gene with protein product
Status	Approved
Aliases	SGDH, CI-SGDH, MGC12314
Ensembl gene	ENSG00000136521
Ensembl biotype	protein_coding
OMIM	603841
Entrez	4711

Gene structure

Transcript identifiers

Ensembl transcripts: 21 — 11 protein_coding, 5 retained_intron, 3 nonsense_mediated_decay, 2 protein_coding_CDS_not_defined

ENST00000259037, ENST00000359944, ENST00000468210, ENST00000471112, ENST00000472629, ENST00000473500, ENST00000476587, ENST00000477177, ENST00000480374, ENST00000482604, ENST00000488002, ENST00000491054, ENST00000493716, ENST00000493866, ENST00000496904, ENST00000611971, ENST00000906708, ENST00000926985, ENST00000926986, ENST00000926987, ENST00000947760

RefSeq mRNA: 3 — MANE Select: NM_002492 NM_001199957, NM_001199958, NM_002492

CCDS: CCDS3234, CCDS56297, CCDS75054

Canonical transcript exons

ENST00000259037 — 6 exons

Exon	Start	End
ENSE00001874142	179623920	179627647
ENSE00001948963	179604794	179604939
ENSE00003492573	179615983	179616049
ENSE00003506204	179614971	179615059
ENSE00003600442	179616983	179617044
ENSE00003626922	179618415	179618521

Expression profiles

Bgee: expression breadth ubiquitous, 303 present calls, max score 99.32.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 173.0284 / max 3146.6910, expressed in 1827 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
39935	172.8492	1827
39934	0.1145	28
39932	0.0518	6
39933	0.0130	5

Top tissues by expression

303 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
heart right ventricle	UBERON:0002080	99.32	gold quality
biceps brachii	UBERON:0001507	99.24	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	99.14	gold quality
pigmented layer of retina	UBERON:0001782	99.01	gold quality
retina	UBERON:0000966	98.98	gold quality
adrenal tissue	UBERON:0018303	98.98	gold quality
cardiac ventricle	UBERON:0002082	98.83	gold quality
heart left ventricle	UBERON:0002084	98.83	gold quality
right atrium auricular region	UBERON:0006631	98.63	gold quality
triceps brachii	UBERON:0001509	98.55	gold quality
cardiac atrium	UBERON:0002081	98.53	gold quality
vastus lateralis	UBERON:0001379	98.46	gold quality
right adrenal gland cortex	UBERON:0035827	98.46	gold quality
hindlimb stylopod muscle	UBERON:0004252	98.40	gold quality
skeletal muscle tissue	UBERON:0001134	98.37	gold quality
right adrenal gland	UBERON:0001233	98.37	gold quality
gastrocnemius	UBERON:0001388	98.37	gold quality
deltoid	UBERON:0001476	98.37	gold quality
heart	UBERON:0000948	98.36	gold quality
rectum	UBERON:0001052	98.36	gold quality
muscle organ	UBERON:0001630	98.35	gold quality
skeletal muscle organ	UBERON:0014892	98.35	gold quality
muscle of leg	UBERON:0001383	98.33	gold quality
left ventricle myocardium	UBERON:0006566	98.33	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	98.27	gold quality
quadriceps femoris	UBERON:0001377	98.26	gold quality
apex of heart	UBERON:0002098	98.25	gold quality
adult organism	UBERON:0007023	98.25	gold quality
monocyte	CL:0000576	98.20	gold quality
diaphragm	UBERON:0001103	98.20	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-MTAB-10042	yes	9.85
E-MTAB-8559	no	537.64
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

25 targeting NDUFB5, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3925-3P	100.00	69.95	1237
HSA-MIR-4668-3P	100.00	68.74	2635
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-548P	99.98	72.25	3784
HSA-MIR-302C-5P	99.97	72.56	3642
HSA-LET-7C-3P	99.95	73.42	2862
HSA-MIR-101-3P	99.94	75.03	2230
HSA-MIR-3143	99.93	71.96	3104
HSA-MIR-139-5P	99.80	69.50	1399
HSA-MIR-4645-3P	99.76	69.33	993
HSA-MIR-187-5P	99.74	70.26	1404
HSA-MIR-4804-3P	99.65	67.78	866
HSA-MIR-671-5P	99.52	67.11	1277
HSA-MIR-543	99.52	69.03	2595
HSA-MIR-3123	99.47	67.15	2693
HSA-MIR-6505-3P	99.34	67.39	1071
HSA-MIR-4311	99.31	70.47	3041
HSA-MIR-7151-3P	99.04	69.72	2370
HSA-MIR-4477A	98.83	69.75	2952
HSA-MIR-6761-5P	98.71	68.03	1504
HSA-MIR-450A-2-3P	97.91	67.56	1459
HSA-MIR-4749-3P	96.40	66.24	798
HSA-MIR-4653-3P	96.26	67.03	725
HSA-MIR-571	95.38	66.54	671
HSA-MIR-7109-3P	94.23	67.19	743

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 14.2% of screened cell lines.

Literature-anchored findings (GeneRIF, showing 3)

• This protein has been found differentially expressed in the temporal lobe from patients with schizophrenia. (PMID:19034380)
• Data suggest that the hub genes NADH:ubiquinone oxidoreductase subunit B5 (NDUFB5), translocase of inner mitochondrial membrane domain containing 1 (TIMMDC1), and voltage-dependent anion channel 3 (VDAC3) might serve as potential biomarkers for diagnosis and/or therapeutic targets for precise treatment of septic cardiomyopathy (SC) . (PMID:31794266)
• METTL3-mediated NDUFB5 m6A modification promotes cell migration and mitochondrial respiration to promote the wound healing of diabetic foot ulcer. (PMID:38982516)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	ndufb5	ENSDARG00000070824
mus_musculus	Ndufb5	ENSMUSG00000027673
rattus_norvegicus	Ndufb5	ENSRNOG00000011949
drosophila_melanogaster	ND-SGDH	FBGN0011455
caenorhabditis_elegans		WBGENE00016118

Protein

Protein identifiers

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial — O43674 (reviewed: O43674)

Alternative names: Complex I-SGDH, NADH-ubiquinone oxidoreductase SGDH subunit

All UniProt accessions (8): A0A087WYD0, E7EWP0, O43674, F8WDD2, F8WDZ2, H0Y886, H7C568, H7C5C1

UniProt curated annotations — full annotation on UniProt →

Function. Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subunit / interactions. Complex I is composed of 45 different subunits.

Subcellular location. Mitochondrion inner membrane.

Similarity. Belongs to the complex I NDUFB5 subunit family.

Isoforms (2)

UniProt ID	Names	Canonical?
O43674-1	1	yes
O43674-2	2

RefSeq proteins (3): NP_001186886, NP_001186887, NP_002483* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR019173	NADH_UbQ_OxRdtase_B5_su	Family

Pfam: PF09781

UniProt features (5 total): transit peptide 1, chain 1, transmembrane region 1, splice variant 1, sequence variant 1

Structure

Experimental structures (PDB)

7 structures.

PDB	Method	Resolution (Å)
9I4I	ELECTRON MICROSCOPY	2.63
9TI4	ELECTRON MICROSCOPY	2.66
9CWT	ELECTRON MICROSCOPY	3.44
5XTC	ELECTRON MICROSCOPY	3.7
5XTD	ELECTRON MICROSCOPY	3.7
5XTH	ELECTRON MICROSCOPY	3.9
5XTI	ELECTRON MICROSCOPY	17.4

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-O43674-F1	84.68	0.70

Function

Pathways and Gene Ontology

Reactome pathways

4 pathways

ID	Pathway
R-HSA-611105	Respiratory electron transport
R-HSA-6799198	Complex I biogenesis
R-HSA-1428517	Aerobic respiration and respiratory electron transport
R-HSA-1430728	Metabolism

MSigDB gene sets: 232 (showing top): MODULE_77, GOBP_ORGANOPHOSPHATE_METABOLIC_PROCESS, TGACCTY_ERR1_Q2, GOBP_NUCLEOSIDE_PHOSPHATE_BIOSYNTHETIC_PROCESS, RIZKI_TUMOR_INVASIVENESS_3D_DN, chr3q26, GOBP_ORGANOPHOSPHATE_BIOSYNTHETIC_PROCESS, GOBP_CARBOHYDRATE_DERIVATIVE_METABOLIC_PROCESS, GOBP_MONOATOMIC_CATION_TRANSPORT, GOBP_NUCLEOBASE_CONTAINING_SMALL_MOLECULE_METABOLIC_PROCESS, GOBP_GENERATION_OF_PRECURSOR_METABOLITES_AND_ENERGY, WATANABE_RECTAL_CANCER_RADIOTHERAPY_RESPONSIVE_UP, GOBP_ATP_BIOSYNTHETIC_PROCESS, GOBP_OXIDATIVE_PHOSPHORYLATION, GOBP_CARBOHYDRATE_DERIVATIVE_BIOSYNTHETIC_PROCESS

GO Biological Process (4): mitochondrial electron transport, NADH to ubiquinone (GO:0006120), aerobic respiration (GO:0009060), proton motive force-driven mitochondrial ATP synthesis (GO:0042776), proton transmembrane transport (GO:1902600)

GO Molecular Function (2): NADH dehydrogenase (ubiquinone) activity (GO:0008137), protein binding (GO:0005515)

GO Cellular Component (5): nucleoplasm (GO:0005654), mitochondrion (GO:0005739), mitochondrial inner membrane (GO:0005743), respiratory chain complex I (GO:0045271), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-3 pathways:

Category	Pathways
Aerobic respiration and respiratory electron transport	1
Respiratory electron transport	1
Metabolism	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
aerobic electron transport chain	1
mitochondrial ATP synthesis coupled electron transport	1
cellular respiration	1
mitochondrion	1
oxidative phosphorylation	1
proton motive force-driven ATP synthesis	1
monoatomic cation transmembrane transport	1
NADH dehydrogenase activity	1
electron transfer activity	1
proton transmembrane transporter activity	1
oxidoreduction-driven active transmembrane transporter activity	1
oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor	1
active monoatomic ion transmembrane transporter activity	1
binding	1
nuclear lumen	1
cytoplasm	1
intracellular membrane-bounded organelle	1
organelle inner membrane	1
mitochondrial membrane	1
NADH dehydrogenase complex	1
respiratory chain complex	1
transmembrane transporter complex	1

Protein interactions and networks

STRING

1924 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
NDUFB5	NDUFB10	O96000	966
NDUFB5	NDUFB3	O43676	947
NDUFB5	NDUFB11	Q9NX14	929
NDUFB5	NDUFA5	Q16718	915
NDUFB5	NDUFA2	O43678	907
NDUFB5	NDUFA6	P56556	904
NDUFB5	NDUFC1	O43677	902
NDUFB5	NDUFS6	O75380	901
NDUFB5	NDUFB1	O75438	897
NDUFB5	NDUFV2	P19404	887
NDUFB5	NDUFB4	O95168	884
NDUFB5	NDUFB6	O95139	884
NDUFB5	NDUFA7	O95182	860
NDUFB5	NDUFA8	P51970	821
NDUFB5	NDUFV3	P56181	820

IntAct

127 interactions, top by confidence:

A	B	Type	Score
NDUFS3	NDUFS8	psi-mi:“MI:0914”(association)	0.730
NDUFS6	NDUFS8	psi-mi:“MI:0914”(association)	0.640
NDUFA13	NDUFS8	psi-mi:“MI:0914”(association)	0.640
ENPP6	SCAMP1	psi-mi:“MI:0914”(association)	0.640
NDUFB5	NDUFB3	psi-mi:“MI:0914”(association)	0.640
NDUFAF4	NDUFS8	psi-mi:“MI:0914”(association)	0.640
NDUFA9	NDUFS4	psi-mi:“MI:0914”(association)	0.640
NDUFB5	SCAND1	psi-mi:“MI:0915”(physical association)	0.560
NDUFS5	NDUFS8	psi-mi:“MI:0914”(association)	0.530
NDUFA9	NDUFS8	psi-mi:“MI:0914”(association)	0.530
TIMMDC1	NDUFS8	psi-mi:“MI:0914”(association)	0.530
LPAR1	TMEM223	psi-mi:“MI:0914”(association)	0.530
C3AR1	TMEM120B	psi-mi:“MI:0914”(association)	0.530
FUT1	GOLIM4	psi-mi:“MI:0914”(association)	0.530
GALNT6	NDUFS4	psi-mi:“MI:0914”(association)	0.530
ITM2A	NDUFB5	psi-mi:“MI:0914”(association)	0.530
IPPK	TMEM223	psi-mi:“MI:0914”(association)	0.530
YIPF3	TMEM120B	psi-mi:“MI:0914”(association)	0.530
NDUFV2	NDUFS8	psi-mi:“MI:0914”(association)	0.530

BioGRID (210): NDUFB5 (Affinity Capture-MS), NDUFB5 (Affinity Capture-MS), NDUFB5 (Affinity Capture-MS), NDUFB5 (Affinity Capture-MS), ATP5F1 (Co-fractionation), EIF3I (Co-fractionation), MTCH2 (Co-fractionation), NDUFS1 (Co-fractionation), UQCRC2 (Co-fractionation), NDUFB5 (Affinity Capture-MS), NDUFB5 (Affinity Capture-MS), NDUFB5 (Affinity Capture-MS), NDUFB5 (Affinity Capture-MS), NDUFB5 (Affinity Capture-MS), NDUFB5 (Affinity Capture-MS)

ESM2 similar proteins: A1XQT2, A3KP48, A8KB87, A8WGU8, O09111, O43674, O49313, O74988, O93980, P00424, P00425, P09669, P0DKM0, P11950, P53077, P80971, Q02376, Q02380, Q0MQ88, Q0MQD6, Q0MQD7, Q0MQD8, Q0MQJ3, Q290M9, Q29DG0, Q2NKR7, Q3T0E3, Q4QQV3, Q4R4E0, Q5R504, Q5R7J0, Q6CCF6, Q6CHT7, Q6CK73, Q6CLM9, Q6DQX6, Q6FW43, Q75FA7, Q7JX57, Q7YRK3

Diamond homologs: O43674, Q02380, Q0MQD6, Q0MQD7, Q0MQD8, Q4R4E0, Q9CQH3

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
NDUFB5	“form complex”	“NADH-ubiquinone oxidoreductase-Mitochondrial respiratory chain complex I”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 112 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Complex I biogenesis	24	50.3×	1e-33
Respiratory electron transport	24	28.9×	3e-27
Aerobic respiration and respiratory electron transport	24	26.9×	1e-26

GO biological processes:

GO term	Partners	Fold	FDR
mitochondrial electron transport, NADH to ubiquinone	18	65.9×	1e-26
proton motive force-driven mitochondrial ATP synthesis	22	59.1×	2e-31
aerobic respiration	22	55.6×	6e-31
mitochondrial respiratory chain complex I assembly	11	46.1×	6e-14

Disease & clinical

Clinical variants and AI predictions

ClinVar

51 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	34
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1454 predictions. Top by Δscore:

Variant	Effect	Δscore
3:179615981:A:AG	acceptor_gain	1.0000
3:179615982:G:GG	acceptor_gain	1.0000
3:179615982:GA:G	acceptor_gain	1.0000
3:179616981:A:AG	acceptor_gain	1.0000
3:179616981:A:G	acceptor_loss	1.0000
3:179616982:G:GA	acceptor_loss	1.0000
3:179616982:G:GG	acceptor_gain	1.0000
3:179617040:ATAAG:A	donor_loss	1.0000
3:179617041:TAAG:T	donor_loss	1.0000
3:179617042:AAG:A	donor_loss	1.0000
3:179617043:AGG:A	donor_loss	1.0000
3:179617044:GG:G	donor_loss	1.0000
3:179617045:G:GC	donor_loss	1.0000
3:179617046:T:G	donor_loss	1.0000
3:179618410:TGTA:T	acceptor_loss	1.0000
3:179618411:GTA:G	acceptor_loss	1.0000
3:179618412:TAGC:T	acceptor_loss	1.0000
3:179618413:A:AG	acceptor_gain	1.0000
3:179618413:AGCAT:A	acceptor_loss	1.0000
3:179618414:G:GG	acceptor_gain	1.0000
3:179618414:GC:G	acceptor_gain	1.0000
3:179618414:GCA:G	acceptor_gain	1.0000
3:179618414:GCAT:G	acceptor_gain	1.0000
3:179618414:GCATC:G	acceptor_gain	1.0000
3:179619100:C:G	donor_gain	1.0000
3:179623918:AG:A	acceptor_gain	1.0000
3:179623919:GG:G	acceptor_gain	1.0000
3:179614557:T:G	donor_gain	0.9900
3:179615056:ATTGG:A	donor_loss	0.9900
3:179615058:TGG:T	donor_loss	0.9900

AlphaMissense

1216 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
3:179618422:T:A	I117K	0.992
3:179617035:A:C	E111D	0.990
3:179617035:A:T	E111D	0.990
3:179616005:G:A	G79E	0.988
3:179617030:T:A	W110R	0.988
3:179617030:T:C	W110R	0.988
3:179618430:T:A	W120R	0.987
3:179618430:T:C	W120R	0.987
3:179616004:G:A	G79R	0.986
3:179616004:G:C	G79R	0.986
3:179618422:T:G	I117R	0.986
3:179618416:A:G	H115R	0.984
3:179618484:G:C	A138P	0.984
3:179616026:T:A	I86K	0.983
3:179617022:C:A	P107Q	0.983
3:179617021:C:T	P107S	0.982
3:179618474:A:C	E134D	0.982
3:179618474:A:T	E134D	0.982
3:179615030:T:C	F62L	0.980
3:179615032:C:A	F62L	0.980
3:179615032:C:G	F62L	0.980
3:179616011:C:G	P81R	0.980
3:179617034:A:T	E111V	0.980
3:179617031:G:T	W110L	0.979
3:179617032:G:C	W110C	0.979
3:179617032:G:T	W110C	0.979
3:179616011:C:A	P81Q	0.978
3:179618417:T:A	H115Q	0.978
3:179618417:T:G	H115Q	0.978
3:179616039:T:A	N90K	0.977

dbSNP variants (sampled 300 via entrez): RS1000033690 (3:179616149 A>G,T), RS1000149418 (3:179615856 T>C), RS1000396752 (3:179608837 T>A), RS1000456215 (3:179615778 T>C), RS1000504213 (3:179622315 T>C), RS1000536743 (3:179611722 A>G), RS1000599819 (3:179611476 G>A,T), RS1000642297 (3:179605703 A>G), RS1000659402 (3:179623001 G>T), RS1000732963 (3:179605335 AAGACAGGTAATATGT>A), RS1000746439 (3:179609173 G>A,C), RS1001109990 (3:179623681 C>A), RS1001112596 (3:179622651 G>A), RS1001162667 (3:179617100 T>A), RS1001210667 (3:179605477 GT>G)

Disease associations

OMIM: gene MIM:603841 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (2): CHEMBL2363065 (PROTEIN COMPLEX), CHEMBL4337 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

8 potent at pChembl≥5 of 20 total, top 8 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
6.06	IC50	870	nM	R-(+)-MARMIN-6’-UNDECANOATE
6.04	IC50	920	nM	R-(+)-MARMIN-6’-LINOLEATE
5.63	IC50	2350	nM	R-(+)-MARMIN-6’-LINOLEATE
5.51	IC50	3080	nM	R-(+)-MARMIN-6’-OCTANOATE
5.43	IC50	3670	nM	R-(+)-MARMIN-6’-UNDECANOATE
5.43	IC50	3710	nM	R-(+)-MARMIN-6’-OCTANOATE
5.31	IC50	4900	nM	(+)-9’-ISOVALEROXYLARICIRESINOL
5.04	IC50	9100	nM	(+)-9’-ISOVALEROXYLARICIRESINOL

PubChem BioAssay actives

8 with measured affinity, of 30 total; 4 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
[(E,3R)-2-hydroxy-2,6-dimethyl-8-(2-oxochromen-7-yl)oxyoct-6-en-3-yl] undecanoate	739270: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	ic50	0.8700	uM
[(E,3R)-2-hydroxy-2,6-dimethyl-8-(2-oxochromen-7-yl)oxyoct-6-en-3-yl] (9Z,12Z)-octadeca-9,12-dienoate	739270: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	ic50	0.9200	uM
[(E,3R)-2-hydroxy-2,6-dimethyl-8-(2-oxochromen-7-yl)oxyoct-6-en-3-yl] octanoate	739270: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	ic50	3.0800	uM
[(2S,3R,4R)-2-(4-hydroxy-3-methoxyphenyl)-4-[(4-hydroxy-3-methoxyphenyl)methyl]oxolan-3-yl]methyl 3-methylbutanoate	739269: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1,10-phenanthroline-induced HIF1 activation incubated for 30 mins prior to 1,10-phenanthroline-challenge measured after 16 hrs by luciferase reporter assay	ic50	4.9000	uM

CTD chemical–gene interactions

35 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	decreases expression, affects cotreatment, increases abundance, increases expression	3
Acetaminophen	affects cotreatment, decreases expression, increases expression	3
bisphenol A	decreases expression, increases expression	2
Arsenic	increases abundance, increases expression, decreases expression, affects cotreatment	2
Particulate Matter	decreases expression, increases abundance, affects cotreatment	2
bisphenol F	increases expression	1
dicrotophos	decreases expression	1
bufotalin	decreases expression	1
O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate	affects expression, affects response to substance	1
tetrahydropalmatine	decreases expression	1
arsenite	affects binding, increases reaction	1
isobutyl alcohol	decreases expression, increases abundance, affects cotreatment	1
di-n-butylphosphoric acid	affects expression	1
chloropicrin	increases expression	1
corosolic acid	decreases expression	1
bisphenol AF	increases expression	1
Air Pollutants	decreases expression, increases abundance	1
Ethanol	decreases expression, increases abundance, affects cotreatment	1
Atrazine	decreases expression	1
Doxorubicin	decreases expression	1
Estradiol	decreases expression	1
Gasoline	affects cotreatment, decreases expression, increases abundance	1
Lipopolysaccharides	affects cotreatment, decreases expression	1
Polycyclic Aromatic Hydrocarbons	affects cotreatment, decreases expression, increases abundance	1
Rotenone	increases expression	1
Tetrachlorodibenzodioxin	decreases expression	1
Thiram	decreases expression	1
Urethane	decreases expression	1
Valproic Acid	affects expression	1
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide	increases expression	1

ChEMBL screening assays

5 unique, capped per target: 5 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL2353025	Binding	Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation at 30 uM incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	Semisynthetic studies identify mitochondria poisons from botanical dietary supplements–geranyloxycoumarins from Aegle marmelos. — Bioorg Med Chem

Cellosaurus cell lines

1 cell lines: 1 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B1YF	Abcam HeLa NDUFB5 KO	Cancer cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.