NDUFB7

Summary

NDUFB7 (NADH:ubiquinone oxidoreductase subunit B7, HGNC:7702) is a protein-coding gene on chromosome 19p13.12, encoding NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (P17568). Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. It is a selective cancer dependency (DepMap: 47.9% of cell lines).

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Source: NCBI Gene 4713 — RefSeq curated summary.

At a glance

• Gene–disease (curated): mitochondrial complex I deficiency, nuclear type 39 (Moderate, GenCC) — +1 more curated relationship
• GWAS associations: 1
• Clinical variants (ClinVar): 30 total — 1 likely-pathogenic
• Phenotypes (HPO): 17
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 47.9% of screened cell lines
• MANE Select transcript: NM_004146

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:7702
Approved symbol	NDUFB7
Name	NADH:ubiquinone oxidoreductase subunit B7
Location	19p13.12
Locus type	gene with protein product
Status	Approved
Aliases	B18, CI-B18, MGC2480
Ensembl gene	ENSG00000099795
Ensembl biotype	protein_coding
OMIM	603842
Entrez	4713

Gene structure

Transcript identifiers

Ensembl transcripts: 4 — 3 protein_coding, 1 nonsense_mediated_decay

ENST00000215565, ENST00000593353, ENST00000897442, ENST00000928508

RefSeq mRNA: 1 — MANE Select: NM_004146 NM_004146

CCDS: CCDS12314

Canonical transcript exons

ENST00000215565 — 3 exons

Exon	Start	End
ENSE00000655642	14566765	14566933
ENSE00003526432	14566078	14566265
ENSE00003845910	14571889	14572066

Expression profiles

Bgee: expression breadth ubiquitous, 285 present calls, max score 99.48.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 107.2537 / max 376.3587, expressed in 1828 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
179667	105.8109	1828
179666	1.4429	815

Top tissues by expression

295 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
apex of heart	UBERON:0002098	99.48	gold quality
mucosa of transverse colon	UBERON:0004991	99.20	gold quality
right atrium auricular region	UBERON:0006631	99.17	gold quality
hindlimb stylopod muscle	UBERON:0004252	98.92	gold quality
muscle layer of sigmoid colon	UBERON:0035805	98.86	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	98.80	gold quality
transverse colon	UBERON:0001157	98.76	gold quality
gastrocnemius	UBERON:0001388	98.74	gold quality
body of stomach	UBERON:0001161	98.72	gold quality
adenohypophysis	UBERON:0002196	98.72	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	98.70	gold quality
left coronary artery	UBERON:0001626	98.67	gold quality
lower esophagus	UBERON:0013473	98.66	gold quality
lower esophagus muscularis layer	UBERON:0035833	98.66	gold quality
metanephros cortex	UBERON:0010533	98.64	gold quality
thoracic aorta	UBERON:0001515	98.59	gold quality
right frontal lobe	UBERON:0002810	98.59	gold quality
left adrenal gland cortex	UBERON:0035825	98.59	gold quality
ascending aorta	UBERON:0001496	98.58	gold quality
triceps brachii	UBERON:0001509	98.58	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	98.58	gold quality
popliteal artery	UBERON:0002250	98.56	gold quality
tibial artery	UBERON:0007610	98.56	gold quality
lower esophagus mucosa	UBERON:0035834	98.56	gold quality
right adrenal gland	UBERON:0001233	98.55	gold quality
left adrenal gland	UBERON:0001234	98.55	gold quality
aorta	UBERON:0000947	98.52	gold quality
muscle of leg	UBERON:0001383	98.51	gold quality
right lobe of thyroid gland	UBERON:0001119	98.50	gold quality
descending thoracic aorta	UBERON:0002345	98.50	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 47.9% of screened cell lines.

Literature-anchored findings (GeneRIF, showing 3)

• Intra-molecular disulfide bridges and the inter-membrane space localization of three Cx(9)C-containing subunits in human: NDUFS5, NDUFB7 and NDUFA8, are proposed. (PMID:21310150)
• This subunit of complex I is localized in the mitochondrial inter-membrane space. The protein contains intra-molecular disulfide bridges in the twin Cx(9)C motif. (PMID:21310150)
• Protein N-myristoylation plays a critical role in the mitochondrial localization of human mitochondrial complex I accessory subunit NDUFB7. (PMID:38151566)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	ndufb7	ENSDARG00000033789
mus_musculus	Ndufb7	ENSMUSG00000033938
rattus_norvegicus	Ndufb7	ENSRNOG00000028717
drosophila_melanogaster	ND-B18	FBGN0030605
caenorhabditis_elegans	ndub-7	WBGENE00008414

Protein

Protein identifiers

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 — P17568 (reviewed: P17568)

Alternative names: Cell adhesion protein SQM1, Complex I-B18, NADH-ubiquinone oxidoreductase B18 subunit

All UniProt accessions (2): P17568, M0R3B8

UniProt curated annotations — full annotation on UniProt →

Function. Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subunit / interactions. Complex I is composed of 45 different subunits.

Subcellular location. Mitochondrion inner membrane. Mitochondrion intermembrane space.

Disease relevance. Mitochondrial complex I deficiency, nuclear type 39 (MC1DN39) [MIM:620135] A form of mitochondrial complex I deficiency, the most common biochemical signature of mitochondrial disorders, a group of highly heterogeneous conditions characterized by defective oxidative phosphorylation, which collectively affects 1 in 5-10000 live births. Clinical disorders have variable severity, ranging from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. MC1DN39 is an autosomal recessive form characterized by intrauterine growth retardation, anemia, and postpartum hypertrophic cardiomyopathy, lactic acidosis, encephalopathy, and a severe complex I defect with a fatal outcome. The disease is caused by variants affecting the gene represented in this entry.

Domain organisation. Contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.

Similarity. Belongs to the complex I NDUFB7 subunit family.

RefSeq proteins (1): NP_004137* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR008698	NDUB7	Family

Pfam: PF05676

UniProt features (11 total): short sequence motif 2, disulfide bond 2, initiator methionine 1, chain 1, sequence variant 1, domain 1, region of interest 1, modified residue 1, lipid moiety-binding region 1

Structure

Experimental structures (PDB)

7 structures.

PDB	Method	Resolution (Å)
9I4I	ELECTRON MICROSCOPY	2.63
9TI4	ELECTRON MICROSCOPY	2.66
9CWT	ELECTRON MICROSCOPY	3.44
5XTC	ELECTRON MICROSCOPY	3.7
5XTD	ELECTRON MICROSCOPY	3.7
5XTH	ELECTRON MICROSCOPY	3.9
5XTI	ELECTRON MICROSCOPY	17.4

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P17568-F1	88.98	0.73

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (2): 73, 2

Disulfide bonds (2): 59–90, 69–80

Function

Pathways and Gene Ontology

Reactome pathways

4 pathways

ID	Pathway
R-HSA-611105	Respiratory electron transport
R-HSA-6799198	Complex I biogenesis
R-HSA-1428517	Aerobic respiration and respiratory electron transport
R-HSA-1430728	Metabolism

MSigDB gene sets: 254 (showing top): MODULE_93, BUYTAERT_PHOTODYNAMIC_THERAPY_STRESS_DN, MODULE_151, MODULE_77, ENK_UV_RESPONSE_KERATINOCYTE_UP, DACOSTA_UV_RESPONSE_VIA_ERCC3_UP, GOBP_ORGANOPHOSPHATE_METABOLIC_PROCESS, GOBP_NUCLEOSIDE_PHOSPHATE_BIOSYNTHETIC_PROCESS, MODULE_308, GOBP_ORGANOPHOSPHATE_BIOSYNTHETIC_PROCESS, GOBP_CARBOHYDRATE_DERIVATIVE_METABOLIC_PROCESS, GOBP_MONOATOMIC_CATION_TRANSPORT, GOBP_NUCLEOBASE_CONTAINING_SMALL_MOLECULE_METABOLIC_PROCESS, GOBP_GENERATION_OF_PRECURSOR_METABOLITES_AND_ENERGY, GALE_APL_WITH_FLT3_MUTATED_DN

GO Biological Process (4): mitochondrial electron transport, NADH to ubiquinone (GO:0006120), aerobic respiration (GO:0009060), proton motive force-driven mitochondrial ATP synthesis (GO:0042776), proton transmembrane transport (GO:1902600)

GO Molecular Function (2): NADH dehydrogenase (ubiquinone) activity (GO:0008137), protein binding (GO:0005515)

GO Cellular Component (5): mitochondrion (GO:0005739), mitochondrial inner membrane (GO:0005743), mitochondrial intermembrane space (GO:0005758), respiratory chain complex I (GO:0045271), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-3 pathways:

Category	Pathways
Aerobic respiration and respiratory electron transport	1
Respiratory electron transport	1
Metabolism	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
aerobic electron transport chain	1
mitochondrial ATP synthesis coupled electron transport	1
cellular respiration	1
mitochondrion	1
oxidative phosphorylation	1
proton motive force-driven ATP synthesis	1
monoatomic cation transmembrane transport	1
NADH dehydrogenase activity	1
electron transfer activity	1
proton transmembrane transporter activity	1
oxidoreduction-driven active transmembrane transporter activity	1
oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor	1
active monoatomic ion transmembrane transporter activity	1
binding	1
cytoplasm	1
intracellular membrane-bounded organelle	1
organelle inner membrane	1
mitochondrial membrane	1
mitochondrial envelope	1
organelle envelope lumen	1
NADH dehydrogenase complex	1
respiratory chain complex	1
transmembrane transporter complex	1
cellular anatomical structure	1

Protein interactions and networks

STRING

3372 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
NDUFB7	NDUFB3	O43676	945
NDUFB7	NDUFA2	O43678	940
NDUFB7	NDUFB8	O95169	852
NDUFB7	NDUFB2	O95178	829
NDUFB7	NDUFB10	O96000	828
NDUFB7	NDUFB9	Q9Y6M9	828
NDUFB7	NDUFV1	P49821	820
NDUFB7	NDUFA8	P51970	807
NDUFB7	NDUFB5	O43674	803
NDUFB7	UQCRH	P07919	799
NDUFB7	NDUFAB1	O14561	787
NDUFB7	NDUFS5	O43920	787
NDUFB7	NDUFS8	O00217	784
NDUFB7	NDUFA13	Q9P0J0	783
NDUFB7	NDUFS6	O75380	778

IntAct

185 interactions, top by confidence:

A	B	Type	Score
NDUFS3	NDUFS8	psi-mi:“MI:0914”(association)	0.730
NDUFS6	NDUFS8	psi-mi:“MI:0914”(association)	0.640
NDUFB5	NDUFB3	psi-mi:“MI:0914”(association)	0.640
NDUFS7	NDUFS8	psi-mi:“MI:0914”(association)	0.640
NDUFA9	NDUFS4	psi-mi:“MI:0914”(association)	0.640
CHCHD4	SSNA1	psi-mi:“MI:0914”(association)	0.640
CLCNKA	NDUFB7	psi-mi:“MI:0915”(physical association)	0.560
ZNF417	NDUFB7	psi-mi:“MI:0915”(physical association)	0.560
TFAP4	NDUFB7	psi-mi:“MI:0915”(physical association)	0.560
ZNF330	NDUFB7	psi-mi:“MI:0915”(physical association)	0.560
CHIC2	NDUFB7	psi-mi:“MI:0915”(physical association)	0.560
CDCA7L	NDUFB7	psi-mi:“MI:0915”(physical association)	0.560
RECK	NDUFB7	psi-mi:“MI:0915”(physical association)	0.560
NFKBID	NDUFB7	psi-mi:“MI:0915”(physical association)	0.560
FAM90A1	NDUFB7	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (151): NDUFB7 (Affinity Capture-MS), ATP5F1 (Co-fractionation), ATP6V0D1 (Co-fractionation), COX4I1 (Co-fractionation), NDUFA10 (Co-fractionation), NDUFB7 (Co-fractionation), NDUFB7 (Co-fractionation), NDUFB9 (Co-fractionation), PHB2 (Co-fractionation), UQCRC2 (Co-fractionation), NDUFB7 (Affinity Capture-MS), NDUFB7 (Affinity Capture-MS), NDUFB7 (Affinity Capture-MS), NDUFB7 (Affinity Capture-MS), NDUFB7 (Affinity Capture-MS)

ESM2 similar proteins: A2BD89, A6ZZI5, B5RTE0, C4YIM0, C5DT65, C5E268, O42921, O43920, O60200, O94581, P0CB87, P0CB88, P0CM70, P0CM71, P17568, P36064, P90789, Q01519, Q02373, Q02379, Q02772, Q0MQE2, Q0MQE3, Q0MQE4, Q0MQH3, Q0MQH4, Q0P3X7, Q0UWF1, Q17Q91, Q1HPL8, Q1K8U2, Q208S3, Q28GG4, Q2M2S5, Q2TAP8, Q3E7A4, Q4R3M6, Q6C4R1, Q756Q5, Q7S4H6

Diamond homologs: P17568, P90789, Q02368, Q0MQE2, Q0MQE3, Q0MQE4, Q54V61, Q9CR61, Q9SKC9

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
NDUFB7	“form complex”	“NADH-ubiquinone oxidoreductase-Mitochondrial respiratory chain complex I”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 92 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Complex I biogenesis	19	52.4×	2e-26
Respiratory electron transport	21	33.3×	3e-25
Aerobic respiration and respiratory electron transport	20	29.5×	5e-23

GO biological processes:

GO term	Partners	Fold	FDR
mitochondrial electron transport, NADH to ubiquinone	17	78.2×	9e-27
proton motive force-driven mitochondrial ATP synthesis	19	64.1×	1e-27
aerobic respiration	19	60.4×	2e-27
mitochondrial respiratory chain complex I assembly	8	42.2×	1e-09

Disease & clinical

Clinical variants and AI predictions

ClinVar

30 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	1
Uncertain significance	21
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (1)

Variant ID	HGVS	Classification
997769	NM_004146.6(NDUFB7):c.113-10C>G	Likely pathogenic

SpliceAI

435 predictions. Top by Δscore:

Variant	Effect	Δscore
19:14566261:CATAG:C	acceptor_gain	1.0000
19:14566262:ATAG:A	acceptor_gain	1.0000
19:14566263:TAG:T	acceptor_gain	1.0000
19:14566264:AG:A	acceptor_gain	1.0000
19:14566266:C:CA	acceptor_loss	1.0000
19:14566266:C:CC	acceptor_gain	1.0000
19:14566759:GCTCA:G	donor_loss	1.0000
19:14566760:CTCAC:C	donor_loss	1.0000
19:14566761:TCA:T	donor_loss	1.0000
19:14566762:CA:C	donor_loss	1.0000
19:14566763:A:AC	donor_gain	1.0000
19:14566763:ACT:A	donor_gain	1.0000
19:14566764:C:CT	donor_gain	1.0000
19:14566764:CT:C	donor_gain	1.0000
19:14566764:CTC:C	donor_gain	1.0000
19:14566764:CTCG:C	donor_gain	1.0000
19:14566929:CATCT:C	acceptor_gain	1.0000
19:14566930:ATCT:A	acceptor_gain	1.0000
19:14566934:C:CC	acceptor_gain	1.0000
19:14571877:A:AC	donor_gain	1.0000
19:14571878:C:CC	donor_gain	1.0000
19:14571888:CCGCG:C	donor_gain	1.0000
19:14571894:T:A	donor_gain	1.0000
19:14566932:CT:C	acceptor_gain	0.9900
19:14566934:CTGCA:C	acceptor_loss	0.9900
19:14571857:C:CA	donor_gain	0.9900
19:14571883:CCTCA:C	donor_loss	0.9900
19:14571884:CTCAC:C	donor_loss	0.9900
19:14571885:TCA:T	donor_loss	0.9900
19:14571886:CA:C	donor_loss	0.9900

AlphaMissense

898 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:14566870:C:G	C59S	0.990
19:14566870:C:T	C59Y	0.990
19:14566871:A:T	C59S	0.990
19:14566241:A:C	F102L	0.988
19:14566241:A:T	F102L	0.988
19:14566243:A:G	F102L	0.988
19:14566840:C:G	C69S	0.988
19:14566841:A:T	C69S	0.988
19:14566777:C:T	C90Y	0.987
19:14566869:G:C	C59W	0.985
19:14566870:C:A	C59F	0.985
19:14566230:C:G	R106P	0.984
19:14566777:C:G	C90S	0.984
19:14566778:A:T	C90S	0.984
19:14566247:C:A	K100N	0.983
19:14566247:C:G	K100N	0.983
19:14566807:C:G	C80S	0.982
19:14566808:A:T	C80S	0.982
19:14566875:G:C	D57E	0.982
19:14566875:G:T	D57E	0.982
19:14566867:G:T	A60D	0.981
19:14566871:A:G	C59R	0.980
19:14566777:C:A	C90F	0.979
19:14566807:C:T	C80Y	0.979
19:14566785:C:A	W87C	0.978
19:14566785:C:G	W87C	0.978
19:14566876:T:A	D57V	0.978
19:14566224:A:G	L108P	0.977
19:14566924:G:T	A41D	0.976
19:14566226:C:A	R107S	0.975

dbSNP variants (sampled 300 via entrez): RS1000068699 (19:14567207 G>A), RS1000190046 (19:14572114 C>T), RS1000242267 (19:14572538 C>T), RS1000560003 (19:14568341 G>A,C), RS1000799953 (19:14568575 G>T), RS1001458783 (19:14573396 G>A), RS1001474696 (19:14568372 C>G,T), RS1001582747 (19:14566628 G>A,C), RS1001769221 (19:14573787 G>A,T), RS1001822836 (19:14569237 A>G), RS1002372327 (19:14574021 C>A,T), RS1002652537 (19:14568031 G>A,C), RS1002853309 (19:14565734 T>C), RS1002984459 (19:14570451 C>T), RS1003285293 (19:14570571 A>C,G,T)

Disease associations

OMIM: gene MIM:603842 | disease phenotypes: MIM:620135

GenCC curated gene-disease

Disease	Classification	Inheritance
mitochondrial complex I deficiency, nuclear type 39	Moderate	Autosomal recessive

ClinGen Gene-Disease Validity (1)

Expert-panel classifications — Definitive > Strong > Moderate > Limited > Disputed > Refuted.

Disease	Classification	Inheritance
mitochondrial disease	Limited	AR

Mondo (1): mitochondrial complex I deficiency, nuclear type 39 (MONDO:0859320)

Orphanet (1): Mitochondrial disorder due to a defect in assembly or maturation of the respiratory chain complexes (Orphanet:309136)

HPO phenotypes

17 total (17 of 17 shown, HPO-id order):

HPO	Term
HP:0000007	Autosomal recessive inheritance
HP:0000028	Cryptorchidism
HP:0000047	Hypospadias
HP:0001511	Intrauterine growth retardation
HP:0001518	Small for gestational age
HP:0001562	Oligohydramnios
HP:0001631	Atrial septal defect
HP:0001639	Hypertrophic cardiomyopathy
HP:0001640	Cardiomegaly
HP:0001903	Anemia
HP:0003128	Lactic acidosis
HP:0006989	Dysplastic corpus callosum
HP:0011682	Perimembranous ventricular septal defect
HP:0011923	Decreased activity of mitochondrial complex I
HP:0012707	Elevated brain lactate level by MRS
HP:0012708	Reduced brain N-acetyl aspartate level by MRS
HP:0034198	Second trimester onset

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST012490_68	Femur bone mineral density x serum urate levels interaction	7.000000e-11

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0004531	urate measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (2): CHEMBL2357 (SINGLE PROTEIN), CHEMBL2363065 (PROTEIN COMPLEX)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

10 potent at pChembl≥5 of 20 total, top 10 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
6.06	IC50	870	nM	R-(+)-MARMIN-6’-UNDECANOATE
6.04	IC50	920	nM	R-(+)-MARMIN-6’-LINOLEATE
5.63	IC50	2350	nM	R-(+)-MARMIN-6’-LINOLEATE
5.51	IC50	3080	nM	R-(+)-MARMIN-6’-OCTANOATE
5.43	IC50	3670	nM	R-(+)-MARMIN-6’-UNDECANOATE
5.43	IC50	3710	nM	R-(+)-MARMIN-6’-OCTANOATE
5.31	IC50	4900	nM	(+)-9’-ISOVALEROXYLARICIRESINOL
5.15	Kd	7124	nM	CHEMBL5653589
5.13	ED50	7411	nM	CHEMBL5653589
5.04	IC50	9100	nM	(+)-9’-ISOVALEROXYLARICIRESINOL

PubChem BioAssay actives

9 with measured affinity, of 30 total; 5 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
[(E,3R)-2-hydroxy-2,6-dimethyl-8-(2-oxochromen-7-yl)oxyoct-6-en-3-yl] undecanoate	739270: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	ic50	0.8700	uM
[(E,3R)-2-hydroxy-2,6-dimethyl-8-(2-oxochromen-7-yl)oxyoct-6-en-3-yl] (9Z,12Z)-octadeca-9,12-dienoate	739270: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	ic50	0.9200	uM
[(E,3R)-2-hydroxy-2,6-dimethyl-8-(2-oxochromen-7-yl)oxyoct-6-en-3-yl] octanoate	739270: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	ic50	3.0800	uM
[(2S,3R,4R)-2-(4-hydroxy-3-methoxyphenyl)-4-[(4-hydroxy-3-methoxyphenyl)methyl]oxolan-3-yl]methyl 3-methylbutanoate	739269: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1,10-phenanthroline-induced HIF1 activation incubated for 30 mins prior to 1,10-phenanthroline-challenge measured after 16 hrs by luciferase reporter assay	ic50	4.9000	uM
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148848: Binding affinity to human NDUFB7 incubated for 45 mins by Kinobead based pull down assay	kd	7.1238	uM

CTD chemical–gene interactions

46 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	increases expression, affects cotreatment, increases abundance	3
Acetaminophen	affects cotreatment, decreases expression	2
Air Pollutants	decreases expression, increases abundance, increases expression	2
Cyclosporine	decreases expression	2
Cadmium Chloride	decreases expression, increases abundance, increases expression	2
Particulate Matter	decreases expression, increases abundance, increases expression	2
FR900359	affects phosphorylation	1
bisphenol F	increases expression	1
bisphenol A	affects expression	1
deoxynivalenol	decreases expression	1
arsenite	affects binding, increases reaction	1
tris(1,3-dichloro-2-propyl)phosphate	decreases expression	1
cobaltous chloride	decreases expression	1
manganese chloride	affects cotreatment, increases abundance, increases expression	1
ochratoxin A	increases expression	1
tris(chloroethyl)phosphate	increases abundance, decreases expression	1
di-n-butylphosphoric acid	affects expression	1
corosolic acid	decreases expression	1
2-palmitoylglycerol	increases expression	1
K 7174	decreases expression	1
mono(2-ethyl-5-hydroxyhexyl) phthalate	increases expression	1
bisphenol B	increases expression	1
bisphenol S	affects expression	1
(+)-JQ1 compound	decreases expression	1
bisphenol AF	increases expression	1
Resveratrol	affects cotreatment, decreases expression	1
Temozolomide	decreases expression	1
Arsenic	increases expression, affects cotreatment, increases abundance	1
Atrazine	decreases expression	1
Cadmium	increases abundance, increases expression	1

ChEMBL screening assays

5 unique, capped per target: 5 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5651890	Binding	Binding affinity to human NDUFB7 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

1 cell lines: 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_D9L3	Ubigene HEK293 NDUFB7 KO	Transformed cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.