NDUFS5

Summary

NDUFS5 (NADH:ubiquinone oxidoreductase subunit S5, HGNC:7712) is a protein-coding gene on chromosome 1p34.3, encoding NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 (O43920). Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. It is a selective cancer dependency (DepMap: 72.2% of cell lines).

This gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. Alternative splicing results in multiple transcript variants and pseudogenes have been identified on chromosomes 1, 4 and 17.

Source: NCBI Gene 4725 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 21 total
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 72.2% of screened cell lines
• MANE Select transcript: NM_004552

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:7712
Approved symbol	NDUFS5
Name	NADH:ubiquinone oxidoreductase subunit S5
Location	1p34.3
Locus type	gene with protein product
Status	Approved
Aliases	CI-15k
Ensembl gene	ENSG00000168653
Ensembl biotype	protein_coding
OMIM	603847
Entrez	4725

Gene structure

Transcript identifiers

Ensembl transcripts: 10 — 10 protein_coding

ENST00000372967, ENST00000372969, ENST00000926958, ENST00000926959, ENST00000926960, ENST00000926961, ENST00000926962, ENST00000926963, ENST00000926964, ENST00000926965

RefSeq mRNA: 2 — MANE Select: NM_004552 NM_001184979, NM_004552

CCDS: CCDS434

Canonical transcript exons

ENST00000372969 — 3 exons

Exon	Start	End
ENSE00001459192	39028723	39028940
ENSE00001459193	39026350	39026402
ENSE00003849021	39034392	39034615

Expression profiles

Bgee: expression breadth ubiquitous, 295 present calls, max score 99.75.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 442.8928 / max 15977.2127, expressed in 1827 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
2257	442.8928	1827

Top tissues by expression

295 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
apex of heart	UBERON:0002098	99.75	gold quality
heart left ventricle	UBERON:0002084	99.67	gold quality
cardiac ventricle	UBERON:0002082	99.66	gold quality
right atrium auricular region	UBERON:0006631	99.66	gold quality
cardiac atrium	UBERON:0002081	99.61	gold quality
cranial nerve II	UBERON:0000941	99.60	gold quality
heart	UBERON:0000948	99.59	gold quality
right frontal lobe	UBERON:0002810	99.58	gold quality
Brodmann (1909) area 9	UBERON:0013540	99.53	gold quality
nucleus accumbens	UBERON:0001882	99.52	gold quality
caudate nucleus	UBERON:0001873	99.50	gold quality
prefrontal cortex	UBERON:0000451	99.49	gold quality
adenohypophysis	UBERON:0002196	99.49	gold quality
amygdala	UBERON:0001876	99.48	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	99.48	gold quality
cingulate cortex	UBERON:0003027	99.46	gold quality
muscle layer of sigmoid colon	UBERON:0035805	99.46	gold quality
lower esophagus muscularis layer	UBERON:0035833	99.46	gold quality
anterior cingulate cortex	UBERON:0009835	99.45	gold quality
lower esophagus	UBERON:0013473	99.45	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	99.45	gold quality
gastrocnemius	UBERON:0001388	99.44	gold quality
muscle of leg	UBERON:0001383	99.43	gold quality
stromal cell of endometrium	CL:0002255	99.42	gold quality
putamen	UBERON:0001874	99.42	gold quality
mucosa of transverse colon	UBERON:0004991	99.42	gold quality
heart right ventricle	UBERON:0002080	99.41	gold quality
hindlimb stylopod muscle	UBERON:0004252	99.41	gold quality
pituitary gland	UBERON:0000007	99.38	gold quality
ganglionic eminence	UBERON:0004023	99.38	gold quality

Single-cell (SCXA)

Detected in 10 experiment(s), a significant marker in 5.

Experiment	Marker?	Max mean expression
E-GEOD-36552	yes	349.05
E-HCAD-6	yes	18.33
E-CURD-122	yes	18.23
E-MTAB-7316	yes	6.73
E-MTAB-8559	no	1189.80
E-CURD-10	no	1067.59
E-MTAB-7606	no	663.82
E-MTAB-8271	no	7.65
E-CURD-114	no	7.19
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

11 targeting NDUFS5, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3173-3P	99.98	66.49	1217
HSA-MIR-6891-5P	99.98	66.53	1372
HSA-MIR-3919	99.87	69.45	2489
HSA-MIR-3941	99.86	70.54	2735
HSA-MIR-3121-3P	99.82	71.96	3630
HSA-MIR-4516	99.61	67.78	3390
HSA-MIR-2392	99.43	67.50	708
HSA-MIR-501-5P	98.77	68.88	1328
HSA-MIR-603	98.58	68.28	1603
HSA-MIR-216B-3P	98.55	67.19	1223
HSA-MIR-4773	98.35	67.30	1710

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 72.2% of screened cell lines.

Literature-anchored findings (GeneRIF, showing 3)

• This subunit of complex I is localized in the mitochondrial inter-membrane space. The protein contains intra-molecular disulfide bridges in the twin Cx(9)C motif. Does not contain an Fe-S cluster (despite what’s suggested by the name). (PMID:21310150)
• Novel myocardial markers GADD45G and NDUFS5 identified by RNA-sequencing predicts left ventricular reverse remodeling in advanced non-ischemic heart failure: a retrospective cohort study. (PMID:32138671)
• AIFM1 is a component of the mitochondrial disulfide relay that drives complex I assembly through efficient import of NDUFS5. (PMID:35859387)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	ndufs5	ENSDARG00000006290
mus_musculus	Ndufs5	ENSMUSG00000028648
rattus_norvegicus	Ndufs5-ps2	ENSRNOG00000026646
rattus_norvegicus	Ndufs5	ENSRNOG00000029339
rattus_norvegicus	Ndufs5-ps1	ENSRNOG00000052764

Protein

Protein identifiers

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 — O43920 (reviewed: O43920)

Alternative names: Complex I-15 kDa, NADH-ubiquinone oxidoreductase 15 kDa subunit

All UniProt accessions (2): O43920, Q6IBA0

UniProt curated annotations — full annotation on UniProt →

Function. Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subunit / interactions. Mammalian complex I is composed of 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.

Subcellular location. Mitochondrion inner membrane. Mitochondrion intermembrane space.

Domain organisation. Contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.

Similarity. Belongs to the complex I NDUFS5 subunit family.

RefSeq proteins (2): NP_001171908, NP_004543* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR019342	NADH_UbQ_OxRdtase_FeS-su5	Family

Pfam: PF10200

UniProt features (8 total): short sequence motif 2, disulfide bond 2, chain 1, domain 1, region of interest 1, sequence variant 1

Structure

Experimental structures (PDB)

7 structures.

PDB	Method	Resolution (Å)
9I4I	ELECTRON MICROSCOPY	2.63
9TI4	ELECTRON MICROSCOPY	2.66
9CWT	ELECTRON MICROSCOPY	3.44
5XTC	ELECTRON MICROSCOPY	3.7
5XTD	ELECTRON MICROSCOPY	3.7
5XTH	ELECTRON MICROSCOPY	3.9
5XTI	ELECTRON MICROSCOPY	17.4

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-O43920-F1	94.51	0.91

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Disulfide bonds (2): 33–66, 43–56

Function

Pathways and Gene Ontology

Reactome pathways

4 pathways

ID	Pathway
R-HSA-611105	Respiratory electron transport
R-HSA-6799198	Complex I biogenesis
R-HSA-1428517	Aerobic respiration and respiratory electron transport
R-HSA-1430728	Metabolism

MSigDB gene sets: 197 (showing top): MODULE_151, MODULE_77, MORF_HDAC1, GOBP_MITOCHONDRIAL_RESPIRATORY_CHAIN_COMPLEX_ASSEMBLY, GOBP_ORGANOPHOSPHATE_METABOLIC_PROCESS, GOBP_NUCLEOSIDE_PHOSPHATE_BIOSYNTHETIC_PROCESS, GOBP_ORGANOPHOSPHATE_BIOSYNTHETIC_PROCESS, GOBP_CARBOHYDRATE_DERIVATIVE_METABOLIC_PROCESS, GOBP_MONOATOMIC_CATION_TRANSPORT, GOBP_NUCLEOBASE_CONTAINING_SMALL_MOLECULE_METABOLIC_PROCESS, GOBP_GENERATION_OF_PRECURSOR_METABOLITES_AND_ENERGY, GOBP_ATP_BIOSYNTHETIC_PROCESS, GOBP_OXIDATIVE_PHOSPHORYLATION, GOBP_CARBOHYDRATE_DERIVATIVE_BIOSYNTHETIC_PROCESS, GOBP_NUCLEOSIDE_TRIPHOSPHATE_METABOLIC_PROCESS

GO Biological Process (5): mitochondrial electron transport, NADH to ubiquinone (GO:0006120), aerobic respiration (GO:0009060), mitochondrial respiratory chain complex I assembly (GO:0032981), proton motive force-driven mitochondrial ATP synthesis (GO:0042776), proton transmembrane transport (GO:1902600)

GO Molecular Function (2): NADH dehydrogenase (ubiquinone) activity (GO:0008137), protein binding (GO:0005515)

GO Cellular Component (5): mitochondrion (GO:0005739), mitochondrial inner membrane (GO:0005743), mitochondrial intermembrane space (GO:0005758), respiratory chain complex I (GO:0045271), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-3 pathways:

Category	Pathways
Aerobic respiration and respiratory electron transport	1
Respiratory electron transport	1
Metabolism	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
aerobic electron transport chain	1
mitochondrial ATP synthesis coupled electron transport	1
cellular respiration	1
NADH dehydrogenase complex assembly	1
mitochondrial respiratory chain complex assembly	1
mitochondrion	1
oxidative phosphorylation	1
proton motive force-driven ATP synthesis	1
monoatomic cation transmembrane transport	1
NADH dehydrogenase activity	1
electron transfer activity	1
proton transmembrane transporter activity	1
oxidoreduction-driven active transmembrane transporter activity	1
oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor	1
active monoatomic ion transmembrane transporter activity	1
binding	1
cytoplasm	1
intracellular membrane-bounded organelle	1
organelle inner membrane	1
mitochondrial membrane	1
mitochondrial envelope	1
organelle envelope lumen	1
NADH dehydrogenase complex	1
respiratory chain complex	1
transmembrane transporter complex	1
cellular anatomical structure	1

Protein interactions and networks

STRING

2000 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
NDUFS5	NDUFS1	P28331	903
NDUFS5	NDUFS6	O75380	894
NDUFS5	NDUFA5	Q16718	880
NDUFS5	NDUFS2	O75306	858
NDUFS5	NDUFS4	O43181	845
NDUFS5	NDUFS3	O75489	838
NDUFS5	NDUFA1	O15239	811
NDUFS5	NDUFA8	P51970	791
NDUFS5	NDUFB7	P17568	787
NDUFS5	NDUFB10	O96000	763
NDUFS5	NDUFA13	Q9P0J0	728
NDUFS5	NDUFA10	O95299	718
NDUFS5	NDUFC2	O95298	717
NDUFS5	NDUFV1	P49821	700
NDUFS5	NDUFB4	O95168	697

IntAct

94 interactions, top by confidence:

A	B	Type	Score
NDUFS3	NDUFS8	psi-mi:“MI:0914”(association)	0.730
NDUFS5	HTT	psi-mi:“MI:0915”(physical association)	0.670
NDUFS6	NDUFS8	psi-mi:“MI:0914”(association)	0.640
NDUFAF4	NDUFS8	psi-mi:“MI:0914”(association)	0.640
NDUFA9	NDUFS4	psi-mi:“MI:0914”(association)	0.640
NDUFAF1	NDUFS5	psi-mi:“MI:0914”(association)	0.640
NDUFS5		psi-mi:“MI:0915”(physical association)	0.560
NDUFS5	ARFGAP3	psi-mi:“MI:0915”(physical association)	0.560
NDUFS5	SGSM1	psi-mi:“MI:0915”(physical association)	0.560
NDUFS5	SPATS1	psi-mi:“MI:0915”(physical association)	0.560
NDUFS5	NDUFS8	psi-mi:“MI:0914”(association)	0.530
TIMMDC1	NDUFS8	psi-mi:“MI:0914”(association)	0.530
NDUFS5	NDUFS4	psi-mi:“MI:0914”(association)	0.530

BioGRID (225): NDUFS5 (Co-fractionation), ECSIT (Affinity Capture-MS), NDUFAF1 (Affinity Capture-MS), ACAD9 (Affinity Capture-MS), TIMMDC1 (Affinity Capture-MS), NDUFB7 (Affinity Capture-MS), NDUFB9 (Affinity Capture-MS), NDUFAF4 (Affinity Capture-MS), TMEM70 (Affinity Capture-MS), NDUFAF3 (Affinity Capture-MS), NDUFB5 (Affinity Capture-MS), NDUFA13 (Affinity Capture-MS), NDUFB6 (Affinity Capture-MS), FOXRED1 (Affinity Capture-MS), NDUFV3 (Affinity Capture-MS)

ESM2 similar proteins: A1L3N6, A6ZMQ6, A9ULB4, B3LM82, B5FXK1, C8ZF59, O42921, O43715, O43920, O60200, O94581, P00429, P0CB87, P0CB88, P0CT19, P14854, P48504, P56277, P56391, Q01519, Q02379, Q0MQH3, Q0MQH4, Q0P451, Q208S3, Q28BU7, Q28CA1, Q2NKR3, Q3E7A9, Q3ZCK8, Q4R374, Q4R3M6, Q53CG4, Q5RCT0, Q5RFJ0, Q6DD38, Q6DHJ6, Q6INR6, Q6YFQ2, Q7S4H6

Diamond homologs: O43920, P0CB87, P0CB88, Q02379, Q0MQH3, Q0MQH4, Q4R3M6, Q99LY9

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
NDUFS5	“form complex”	“NADH-ubiquinone oxidoreductase-Mitochondrial respiratory chain complex I”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 82 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Complex I biogenesis	20	56.1×	1e-28
Respiratory electron transport	19	30.6×	8e-22
Aerobic respiration and respiratory electron transport	18	27.0×	1e-19
Mitochondrial protein degradation	5	9.7×	7e-03

GO biological processes:

GO term	Partners	Fold	FDR
mitochondrial electron transport, NADH to ubiquinone	15	78.0×	1e-22
mitochondrial respiratory chain complex I assembly	10	59.6×	1e-13
proton motive force-driven mitochondrial ATP synthesis	15	57.2×	1e-20
aerobic respiration	15	53.9×	2e-20

Disease & clinical

Clinical variants and AI predictions

ClinVar

21 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	12
Likely benign	2
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

291 predictions. Top by Δscore:

Variant	Effect	Δscore
1:39026400:T:A	donor_loss	1.0000
1:39028720:C:G	acceptor_gain	1.0000
1:39028721:A:AG	acceptor_gain	1.0000
1:39028721:AGCC:A	acceptor_loss	1.0000
1:39028722:G:GG	acceptor_gain	1.0000
1:39028722:GC:G	acceptor_gain	1.0000
1:39028722:GCC:G	acceptor_gain	1.0000
1:39028722:GCCA:G	acceptor_gain	1.0000
1:39028722:GCCAT:G	acceptor_gain	1.0000
1:39028938:ACGG:A	donor_loss	1.0000
1:39028940:GGTA:G	donor_loss	1.0000
1:39028941:G:A	donor_loss	1.0000
1:39028941:G:GG	donor_gain	1.0000
1:39028942:T:G	donor_loss	1.0000
1:39026397:GG:G	donor_gain	0.9900
1:39026398:GG:G	donor_gain	0.9900
1:39026399:G:GG	donor_gain	0.9900
1:39028707:A:AG	acceptor_gain	0.9900
1:39028708:A:G	acceptor_gain	0.9900
1:39028716:A:AG	acceptor_gain	0.9900
1:39028717:T:G	acceptor_gain	0.9900
1:39028719:A:AG	acceptor_gain	0.9900
1:39028938:ACG:A	donor_gain	0.9900
1:39034387:GACA:G	acceptor_loss	0.9900
1:39034388:ACAGA:A	acceptor_loss	0.9900
1:39034389:CAG:C	acceptor_loss	0.9900
1:39034390:A:AG	acceptor_gain	0.9900
1:39034390:AG:A	acceptor_loss	0.9900
1:39034390:AGAT:A	acceptor_gain	0.9900
1:39034391:G:GA	acceptor_gain	0.9900

AlphaMissense

698 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:39028821:T:A	C33S	0.988
1:39028822:G:C	C33S	0.988
1:39028890:T:A	C56S	0.987
1:39028891:G:C	C56S	0.987
1:39028821:T:C	C33R	0.986
1:39028830:T:C	F36L	0.986
1:39028832:T:A	F36L	0.986
1:39028832:T:G	F36L	0.986
1:39028842:T:A	W40R	0.984
1:39028842:T:C	W40R	0.984
1:39028851:T:A	C43S	0.982
1:39028852:G:C	C43S	0.982
1:39028920:T:C	C66R	0.981
1:39028822:G:A	C33Y	0.978
1:39028890:T:C	C56R	0.978
1:39028891:G:A	C56Y	0.978
1:39028892:C:G	C56W	0.978
1:39034398:C:A	R75S	0.978
1:39028854:G:C	A44P	0.977
1:39028910:T:A	D62E	0.977
1:39028910:T:G	D62E	0.977
1:39028922:T:G	C66W	0.976
1:39028851:T:C	C43R	0.975
1:39028831:T:G	F36C	0.974
1:39028891:G:T	C56F	0.974
1:39028823:C:G	C33W	0.973
1:39028920:T:A	C66S	0.973
1:39028921:G:C	C66S	0.973
1:39028822:G:T	C33F	0.972
1:39028878:G:C	A52P	0.972

dbSNP variants (sampled 300 via entrez): RS1000034728 (1:39031936 T>C), RS1000375737 (1:39025453 C>T), RS1000477408 (1:39028507 T>C), RS1000534158 (1:39028252 T>C), RS1000685340 (1:39025091 G>A), RS1000721152 (1:39034891 G>A,C), RS1001086632 (1:39034560 G>A), RS1001117403 (1:39026742 C>G,T), RS1001364043 (1:39029571 A>G), RS1001581421 (1:39031441 T>G), RS1001725620 (1:39031843 T>C), RS1002063798 (1:39033389 T>C), RS1002121657 (1:39026185 C>G), RS1002432226 (1:39027566 A>G,T), RS1002557754 (1:39025925 T>C)

Disease associations

OMIM: gene MIM:603847 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST003475_2	Beard thickness	8.000000e-06

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL2363065 (PROTEIN COMPLEX)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

8 potent at pChembl≥5 of 18 total, top 8 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
6.06	IC50	870	nM	R-(+)-MARMIN-6’-UNDECANOATE
6.04	IC50	920	nM	R-(+)-MARMIN-6’-LINOLEATE
5.63	IC50	2350	nM	R-(+)-MARMIN-6’-LINOLEATE
5.51	IC50	3080	nM	R-(+)-MARMIN-6’-OCTANOATE
5.43	IC50	3670	nM	R-(+)-MARMIN-6’-UNDECANOATE
5.43	IC50	3710	nM	R-(+)-MARMIN-6’-OCTANOATE
5.31	IC50	4900	nM	(+)-9’-ISOVALEROXYLARICIRESINOL
5.04	IC50	9100	nM	(+)-9’-ISOVALEROXYLARICIRESINOL

PubChem BioAssay actives

8 with measured affinity, of 28 total; 4 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
[(E,3R)-2-hydroxy-2,6-dimethyl-8-(2-oxochromen-7-yl)oxyoct-6-en-3-yl] undecanoate	739270: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	ic50	0.8700	uM
[(E,3R)-2-hydroxy-2,6-dimethyl-8-(2-oxochromen-7-yl)oxyoct-6-en-3-yl] (9Z,12Z)-octadeca-9,12-dienoate	739270: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	ic50	0.9200	uM
[(E,3R)-2-hydroxy-2,6-dimethyl-8-(2-oxochromen-7-yl)oxyoct-6-en-3-yl] octanoate	739270: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	ic50	3.0800	uM
[(2S,3R,4R)-2-(4-hydroxy-3-methoxyphenyl)-4-[(4-hydroxy-3-methoxyphenyl)methyl]oxolan-3-yl]methyl 3-methylbutanoate	739269: Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1,10-phenanthroline-induced HIF1 activation incubated for 30 mins prior to 1,10-phenanthroline-challenge measured after 16 hrs by luciferase reporter assay	ic50	4.9000	uM

CTD chemical–gene interactions

39 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	decreases expression, increases expression	3
bisphenol A	affects expression, decreases expression	2
Acetaminophen	affects cotreatment, decreases expression, affects expression	2
Air Pollutants	decreases expression, increases abundance	2
Particulate Matter	decreases expression, increases abundance	2
dicrotophos	decreases expression	1
triphenyl phosphate	affects expression	1
deoxynivalenol	decreases expression	1
arsenite	affects binding, increases reaction	1
methylparaben	decreases expression	1
perfluorooctanoic acid	decreases expression	1
CD 437	decreases expression	1
chloropicrin	affects expression	1
K 7174	decreases expression	1
3-(4’-hydroxy-3’-adamantylbiphenyl-4-yl)acrylic acid	decreases expression	1
Sunitinib	decreases expression	1
Atrazine	decreases expression	1
Cadmium	increases abundance, increases expression	1
Doxorubicin	affects expression	1
Hydralazine	affects cotreatment, increases expression	1
Isoniazid	decreases expression	1
Ivermectin	decreases expression	1
Lipopolysaccharides	affects cotreatment, decreases expression	1
Mitoxantrone	affects response to substance	1
Phenobarbital	decreases expression	1
Smoke	decreases expression	1
Thiram	decreases expression	1
Tobacco Smoke Pollution	increases expression	1
Tretinoin	affects cotreatment, increases expression	1
Tunicamycin	decreases expression	1

ChEMBL screening assays

4 unique, capped per target: 4 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL2353025	Binding	Inhibition of mitochondrial ETC complex 1 in human T47D cells assessed as inhibition of 1% O2-induced HIF1 activation at 30 uM incubated for 30 mins prior to 1% O2-challenge measured after 16 hrs by luciferase reporter assay	Semisynthetic studies identify mitochondria poisons from botanical dietary supplements–geranyloxycoumarins from Aegle marmelos. — Bioorg Med Chem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.