NFILZ

Summary

NFILZ (NFIL3 like basic leucine zipper, HGNC:52681) is a protein-coding gene on chromosome 19p13.2, encoding NFIL3 like protein (A0A5F9ZHS7).

Predicted to enable DNA binding activity and DNA-binding transcription factor activity. Predicted to be involved in circadian rhythm and regulation of DNA-templated transcription. Predicted to be active in nucleus.

Source: NCBI Gene 105372267 — RefSeq curated summary.

At a glance

• MANE Select transcript: NM_001378600

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:52681
Approved symbol	NFILZ
Name	NFIL3 like basic leucine zipper
Location	19p13.2
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000268480
Ensembl biotype	protein_coding
Entrez	105372267

Gene structure

Transcript identifiers

Ensembl transcripts: 6 — 4 protein_coding, 2 protein_coding_CDS_not_defined

ENST00000570582, ENST00000593792, ENST00000663339, ENST00000671902, ENST00000673603, ENST00000691075

RefSeq mRNA: 3 — MANE Select: NM_001378600 NM_001378599, NM_001378600, NM_001378601

CCDS: CCDS92505

Canonical transcript exons

ENST00000691075 — 6 exons

Exon	Start	End
ENSE00002640251	8676751	8681151
ENSE00003147153	8635650	8635746
ENSE00003172134	8632476	8632625
ENSE00003888709	8674551	8674600
ENSE00003891378	8676359	8676456
ENSE00003933609	8630633	8630744

Expression profiles

Bgee: expression breadth broad, 48 present calls, max score 91.24.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.0439 / max 15.7880, expressed in 20 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
173688	0.0301	17
173687	0.0137	3

Top tissues by expression

93 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
thymus	UBERON:0002370	91.24	silver quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	89.23	gold quality
cerebellar vermis	UBERON:0004720	81.72	gold quality
quadriceps femoris	UBERON:0001377	79.70	gold quality
left testis	UBERON:0004533	70.05	gold quality
testis	UBERON:0000473	69.88	gold quality
right testis	UBERON:0004534	69.22	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	68.08	gold quality
granulocyte	CL:0000094	49.49	silver quality
endometrium	UBERON:0001295	46.83	gold quality
placenta	UBERON:0001987	43.82	gold quality
stromal cell of endometrium	CL:0002255	43.26	silver quality
mucosa of transverse colon	UBERON:0004991	42.14	silver quality
blood	UBERON:0000178	42.10	gold quality
vermiform appendix	UBERON:0001154	39.81	silver quality
lymph node	UBERON:0000029	39.44	gold quality
bone marrow	UBERON:0002371	38.91	silver quality
bone marrow cell	CL:0002092	38.51	gold quality
calcaneal tendon	UBERON:0003701	38.39	gold quality
colonic epithelium	UBERON:0000397	37.20	gold quality
ventricular zone	UBERON:0003053	36.48	gold quality
cortical plate	UBERON:0005343	36.47	gold quality
leukocyte	CL:0000738	36.20	silver quality
hindlimb stylopod muscle	UBERON:0004252	35.92	silver quality
ganglionic eminence	UBERON:0004023	35.49	gold quality
liver	UBERON:0002107	34.93	silver quality
gall bladder	UBERON:0002110	34.38	silver quality
monocyte	CL:0000576	34.33	gold quality
skeletal muscle tissue	UBERON:0001134	34.04	silver quality
rectum	UBERON:0001052	32.79	silver quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-MTAB-6678	yes	25.46
E-ANND-3	no	1.48

Regulation

Is transcription factor: no

Cross-species orthologs

6 orthologs

Organism	Symbol	Gene ID
danio_rerio	si:dkey-172o19.2	ENSDARG00000071398
danio_rerio	nfil3-4	ENSDARG00000092346
mus_musculus	Nfilz	ENSMUSG00000117495
rattus_norvegicus	Nfilz	ENSRNOG00000063608
drosophila_melanogaster	vri	FBGN0016076
caenorhabditis_elegans		WBGENE00000220

Paralogs (1): NFIL3 (ENSG00000165030)

Protein

Protein identifiers

NFIL3 like protein — A0A5F9ZHS7 (reviewed: A0A5F9ZHS7)

Alternative names: NFIL3 like basic leucine zipper

All UniProt accessions (1): A0A5F9ZHS7

UniProt curated annotations — full annotation on UniProt →

Subcellular location. Nucleus.

Similarity. Belongs to the bZIP family. NFIL3 subfamily.

RefSeq proteins (3): NP_001365528, NP_001365529, NP_001365530 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR004827	bZIP	Domain
IPR046347	bZIP_sf	Homologous_superfamily
IPR047106	NFIL3-like_bZIP	Domain
IPR047229	NFIL3-like	Family

Pfam: PF07716

UniProt features (5 total): region of interest 3, chain 1, domain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-A0A5F9ZHS7-F1	59.84	0.20

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 8 (showing top): GOBP_CIRCADIAN_RHYTHM, chr19p13, GOBP_RHYTHMIC_PROCESS, GOMF_TRANSCRIPTION_REGULATOR_ACTIVITY, SMN1_SMN2_TARGET_GENES, DESCARTES_MAIN_FETAL_ELF3_AGBL2_POSITIVE_CELLS, GOMF_DNA_BINDING_TRANSCRIPTION_FACTOR_ACTIVITY, ZNF248_TARGET_GENES

GO Biological Process (2): regulation of DNA-templated transcription (GO:0006355), circadian rhythm (GO:0007623)

GO Molecular Function (2): DNA binding (GO:0003677), DNA-binding transcription factor activity (GO:0003700)

GO Cellular Component (1): nucleus (GO:0005634)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
DNA-templated transcription	1
regulation of gene expression	1
regulation of RNA biosynthetic process	1
rhythmic process	1
nucleic acid binding	1
transcription cis-regulatory region binding	1
regulation of DNA-templated transcription	1
transcription regulator activity	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

498 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
NFILZ	CEBPE	Q15744	353
NFILZ	NRL	P54845	353
NFILZ	USF1	P22415	348
NFILZ	NR2F1	P10589	338
NFILZ	CEBPD	P49716	333
NFILZ	BACE2	Q9Y5Z0	307
NFILZ	TIMELESS	Q9UNS1	306
NFILZ	F8	P00451	253
NFILZ	BMAL2	Q8WYA1	244
NFILZ	BMAL1	O00327	244
NFILZ	CRY1	Q16526	241
NFILZ	CRY2	Q49AN0	241
NFILZ	DDIT4	Q9NX09	223
NFILZ	NAA80	Q93015	213
NFILZ	GSK3A	P49840	200
NFILZ	GSK3B	P49841	200

IntAct

0 interactions, top by confidence:

ESM2 similar proteins: A0A1B0GUS0, A0A5F9ZHS7, A7E346, A7MB34, A8MZG2, B2RU40, D4A9R4, O08574, O75593, P0C1Z6, P0CG20, Q0VG99, Q0ZCJ7, Q17QH7, Q29RM2, Q2KIS6, Q2M2S6, Q2M3G4, Q2NL68, Q32LE6, Q3U1J1, Q5JXC2, Q5R815, Q5SW24, Q61660, Q63247, Q6NZ36, Q6PBC9, Q6ZN01, Q6ZRI6, Q7TN08, Q7Z591, Q80VF6, Q86WR7, Q8BG26, Q8BP99, Q8BXQ8, Q8IYS4, Q8N9Y4, Q8NAV2

Diamond homologs: A0A5F9ZHS7, O08750, P16443, P41224, P97516, Q08D88, Q10586, Q10587, Q16534, Q16649, Q32PF6, Q5FW38, Q60925, Q64709, Q66J36, Q68EL6, Q6IMZ0, Q8BW74, Q90Z72, Q92172, Q94126, Q9JLC6

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

0 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	0
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

0 predictions. Top by Δscore:

AlphaMissense

0 scored. Top likely-pathogenic:

dbSNP variants (sampled 300 via entrez): RS1000060030 (19:8658096 T>A), RS1000094141 (19:8664358 C>T), RS1000278136 (19:8681420 A>T), RS1000311669 (19:8642210 T>C), RS1000411088 (19:8635961 A>C,G,T), RS1000484704 (19:8636167 C>T), RS1000490178 (19:8676123 A>G), RS1000528316 (19:8664495 C>G,T), RS1000574209 (19:8654288 A>C), RS1000630646 (19:8648590 G>C), RS1000660410 (19:8648941 A>G), RS1000776651 (19:8648194 A>G,T), RS1000874457 (19:8631676 G>A,T), RS1000928252 (19:8671170 G>T), RS1000929074 (19:8670949 T>C)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.