NFXL1

Summary

NFXL1 (nuclear transcription factor, X-box binding like 1, HGNC:18726) is a protein-coding gene on chromosome 4p12, encoding NF-X1-type zinc finger protein NFXL1 (Q6ZNB6).

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of DNA-templated transcription. Located in membrane.

Source: NCBI Gene 152518 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 72 total
• Druggable target: yes
• MANE Select transcript: NM_001278624

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:18726
Approved symbol	NFXL1
Name	nuclear transcription factor, X-box binding like 1
Location	4p12
Locus type	gene with protein product
Status	Approved
Aliases	HOZFP
Ensembl gene	ENSG00000170448
Ensembl biotype	protein_coding
OMIM	620488
Entrez	152518

Gene structure

Transcript identifiers

Ensembl transcripts: 11 — 6 protein_coding, 4 retained_intron, 1 nonsense_mediated_decay

ENST00000329043, ENST00000381538, ENST00000464756, ENST00000502448, ENST00000507131, ENST00000507489, ENST00000508115, ENST00000511452, ENST00000906325, ENST00000927224, ENST00000927225

RefSeq mRNA: 3 — MANE Select: NM_001278624 NM_001278623, NM_001278624, NM_152995

CCDS: CCDS3478

Canonical transcript exons

ENST00000507489 — 23 exons

Exon	Start	End
ENSE00002029364	47914365	47914583
ENSE00003459175	47851095	47851148
ENSE00003477579	47878525	47878665
ENSE00003495571	47862846	47862915
ENSE00003498340	47885498	47885657
ENSE00003501264	47913969	47914205
ENSE00003502489	47851856	47851942
ENSE00003506795	47898757	47898857
ENSE00003515939	47879096	47879117
ENSE00003516958	47905237	47905346
ENSE00003557132	47903193	47903323
ENSE00003559443	47890613	47890703
ENSE00003559777	47855059	47855163
ENSE00003564327	47898959	47899120
ENSE00003587070	47875127	47875293
ENSE00003589130	47884346	47884437
ENSE00003618586	47894180	47894302
ENSE00003636057	47899370	47899548
ENSE00003644841	47896523	47896647
ENSE00003647644	47897967	47898081
ENSE00003648993	47885879	47885999
ENSE00003657537	47847240	47848336
ENSE00003689644	47910824	47910994

Expression profiles

Bgee: expression breadth ubiquitous, 241 present calls, max score 97.87.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 13.3563 / max 186.3378, expressed in 1766 samples.

FANTOM5 promoters (6 alternative TSS)

Promoter ID	TPM avg	Samples expressed
52046	7.8458	1698
52045	2.6720	1307
52047	2.4210	1188
52043	0.1627	45
52044	0.1450	47
52042	0.1098	33

Top tissues by expression

248 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
secondary oocyte	CL:0000655	97.87	gold quality
oocyte	CL:0000023	95.55	gold quality
epithelial cell of pancreas	CL:0000083	93.38	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	92.91	gold quality
sperm	CL:0000019	88.66	gold quality
corpus epididymis	UBERON:0004359	87.75	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	86.94	gold quality
calcaneal tendon	UBERON:0003701	86.46	gold quality
cartilage tissue	UBERON:0002418	86.31	gold quality
ventricular zone	UBERON:0003053	85.04	gold quality
body of pancreas	UBERON:0001150	84.71	gold quality
testis	UBERON:0000473	84.14	gold quality
left testis	UBERON:0004533	83.73	gold quality
endothelial cell	CL:0000115	83.62	silver quality
right testis	UBERON:0004534	83.50	gold quality
bone marrow cell	CL:0002092	83.05	gold quality
pancreas	UBERON:0001264	82.87	gold quality
endometrium	UBERON:0001295	82.81	gold quality
right lobe of liver	UBERON:0001114	82.73	gold quality
caput epididymis	UBERON:0004358	82.58	gold quality
colonic epithelium	UBERON:0000397	81.98	gold quality
right lobe of thyroid gland	UBERON:0001119	81.81	gold quality
oviduct epithelium	UBERON:0004804	81.77	gold quality
left lobe of thyroid gland	UBERON:0001120	81.57	gold quality
thyroid gland	UBERON:0002046	81.55	gold quality
right uterine tube	UBERON:0001302	81.30	gold quality
tendon	UBERON:0000043	81.28	gold quality
endocervix	UBERON:0000458	81.14	gold quality
pancreatic ductal cell	CL:0002079	81.02	silver quality
body of uterus	UBERON:0009853	81.02	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	6.10
E-ENAD-17	no	247.30

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

49 targeting NFXL1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-126-5P	100.00	72.71	3180
HSA-MIR-4682	100.00	68.89	1258
HSA-MIR-4795-3P	100.00	74.62	4024
HSA-MIR-6833-3P	100.00	70.63	3197
HSA-MIR-4768-5P	100.00	69.49	2861
HSA-MIR-3646	100.00	73.56	5283
HSA-MIR-5692B	100.00	71.32	2622
HSA-MIR-5692C	100.00	71.32	2622
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-196A-1-3P	99.99	72.15	2772
HSA-MIR-548P	99.98	72.25	3784
HSA-MIR-607	99.97	73.62	5593
HSA-MIR-302C-5P	99.97	72.56	3642
HSA-MIR-570-3P	99.96	72.41	4910
HSA-MIR-3658	99.96	73.87	4379
HSA-MIR-548AT-5P	99.96	70.83	2666
HSA-MIR-144-3P	99.94	73.98	2698
HSA-MIR-6835-3P	99.93	70.49	2904
HSA-MIR-6809-3P	99.91	71.45	3814
HSA-MIR-548E-5P	99.89	72.73	4486
HSA-MIR-576-5P	99.84	70.46	2582
HSA-MIR-1323	99.83	69.89	2471
HSA-MIR-548AZ-5P	99.83	69.94	3230
HSA-MIR-548T-5P	99.83	69.91	3220
HSA-MIR-944	99.82	70.85	3042
HSA-MIR-5002-5P	99.76	70.84	1763
HSA-MIR-548O-3P	99.74	69.30	2228
HSA-MIR-4428	99.73	66.41	1733
HSA-MIR-488-3P	99.61	68.79	1731

Literature-anchored findings (GeneRIF, showing 1)

• We conclude that coding variants within NFXL1 confer an increased risk of Specific Language Impairment within a complex genetic model (PMID:25781923)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	nfxl1	ENSDARG00000070127
mus_musculus	Nfxl1	ENSMUSG00000072889
rattus_norvegicus	Nfxl1	ENSRNOG00000058588
drosophila_melanogaster	CG15011	FBGN0035518

Paralogs (1): NFX1 (ENSG00000086102)

Protein

Protein identifiers

NF-X1-type zinc finger protein NFXL1 — Q6ZNB6 (reviewed: Q6ZNB6)

Alternative names: Ovarian zinc finger protein

All UniProt accessions (1): Q6ZNB6

UniProt curated annotations — full annotation on UniProt →

Subcellular location. Membrane.

Miscellaneous. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Similarity. Belongs to the NFX1 family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q6ZNB6-1	1	yes
Q6ZNB6-2	2

RefSeq proteins (3): NP_001265552, NP_001265553, NP_694540 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000967	Znf_NFX1	Domain
IPR001841	Znf_RING	Domain
IPR034078	NFX1_fam	Family

Pfam: PF01422

UniProt features (26 total): zinc finger region 11, modified residue 6, splice variant 2, chain 1, transmembrane region 1, region of interest 1, coiled-coil region 1, compositionally biased region 1, sequence variant 1, sequence conflict 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q6ZNB6-F1	74.40	0.07

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (6): 50, 65, 106, 107, 108, 109

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 120 (showing top): RRAGTTGT_UNKNOWN, GCAAGGA_MIR502, chr4p12, FOSTER_TOLERANT_MACROPHAGE_UP, ATGTCAC_MIR489, ACTWSNACTNY_UNKNOWN, CTGAGCC_MIR24, GOMF_SEQUENCE_SPECIFIC_DNA_BINDING, CHICAS_RB1_TARGETS_GROWING, SNACANNNYSYAGA_UNKNOWN, MTOR_UP.V1_DN, STK33_SKM_UP, STK33_UP, GOMF_TRANSCRIPTION_REGULATOR_ACTIVITY, E2F5_TARGET_GENES

GO Biological Process (2): regulation of DNA-templated transcription (GO:0006355), regulation of transcription by RNA polymerase II (GO:0006357)

GO Molecular Function (5): RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977), DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981), zinc ion binding (GO:0008270), DNA-binding transcription factor activity (GO:0003700), metal ion binding (GO:0046872)

GO Cellular Component (3): chromatin (GO:0000785), nucleus (GO:0005634), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
regulation of DNA-templated transcription	2
transcription cis-regulatory region binding	2
cellular anatomical structure	2
DNA-templated transcription	1
regulation of gene expression	1
regulation of RNA biosynthetic process	1
transcription by RNA polymerase II	1
chromatin	1
RNA polymerase II transcription regulatory region sequence-specific DNA binding	1
DNA-binding transcription factor activity	1
regulation of transcription by RNA polymerase II	1
transition metal ion binding	1
transcription regulator activity	1
cation binding	1
chromosome	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

1273 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
NFXL1	ATP2C2	O75185	690
NFXL1	CMIP	Q8IY22	649
NFXL1	DNAAF4	Q8WXU2	578
NFXL1	KIAA0319	Q5VV43	571
NFXL1	DCDC2	Q9UHG0	563
NFXL1	CNTNAP2	Q9UHC6	561
NFXL1	ROBO2	Q9HCK4	508
NFXL1	SLAIN2	Q9P270	496
NFXL1	CNGA1	P29973	494
NFXL1	OCIAD1	Q9NX40	471
NFXL1	ROBO1	Q9Y6N7	459
NFXL1	NIPAL1	Q6NVV3	447
NFXL1	SLC10A4	Q96EP9	433
NFXL1	LRRC66	Q68CR7	418
NFXL1	ZNF451	Q9Y4E5	416

IntAct

93 interactions, top by confidence:

A	B	Type	Score
TUBA1C	TXNDC9	psi-mi:“MI:0914”(association)	0.730
KLK5	DENND11	psi-mi:“MI:0914”(association)	0.640
SLC1A1	AGPAT2	psi-mi:“MI:0914”(association)	0.640
EGFR	NDUFA4	psi-mi:“MI:0914”(association)	0.530
RPN1	APBB1	psi-mi:“MI:0914”(association)	0.530
TUBB3	POTEF	psi-mi:“MI:0914”(association)	0.530
TUBA4A	PLD2	psi-mi:“MI:0914”(association)	0.530
NCEH1	CLGN	psi-mi:“MI:0914”(association)	0.530
GALNS	CLGN	psi-mi:“MI:0914”(association)	0.530
TUBA1A	TUBAL3	psi-mi:“MI:0914”(association)	0.420
NFXL1	TOMM20	psi-mi:“MI:0915”(physical association)	0.400
NFXL1	TPR	psi-mi:“MI:0915”(physical association)	0.400
FOXL1	IFRD1	psi-mi:“MI:0914”(association)	0.350
RIPK4	VWA8	psi-mi:“MI:0914”(association)	0.350
HSCB	RBP5	psi-mi:“MI:0914”(association)	0.350
SLC16A11	ESYT2	psi-mi:“MI:0914”(association)	0.350
NTRK1	ILVBL	psi-mi:“MI:0914”(association)	0.350
NTRK3	ILVBL	psi-mi:“MI:0914”(association)	0.350
GRHL1	POLRMT	psi-mi:“MI:0914”(association)	0.350
CANX	HLA-A	psi-mi:“MI:0914”(association)	0.350
DDOST	ATL3	psi-mi:“MI:0914”(association)	0.350
CCDC47	ESYT2	psi-mi:“MI:0914”(association)	0.350
OST4	ATL3	psi-mi:“MI:0914”(association)	0.350
RPN2	APBB1	psi-mi:“MI:0914”(association)	0.350
MGARP	BTAF1	psi-mi:“MI:0914”(association)	0.350
GLMP	RTL8C	psi-mi:“MI:0914”(association)	0.350
FFAR1	SLC12A8	psi-mi:“MI:0914”(association)	0.350
GPR182	SLC12A8	psi-mi:“MI:0914”(association)	0.350
RCCD1	IFT56	psi-mi:“MI:0914”(association)	0.350

BioGRID (152): NFXL1 (Affinity Capture-MS), NFXL1 (Affinity Capture-MS), NFXL1 (Affinity Capture-MS), NFXL1 (Reconstituted Complex), NFXL1 (Affinity Capture-MS), NFXL1 (Affinity Capture-MS), NFXL1 (Affinity Capture-MS), NFXL1 (Affinity Capture-MS), NFXL1 (Affinity Capture-MS), NFXL1 (Affinity Capture-MS), NFXL1 (Proximity Label-MS), NFXL1 (Affinity Capture-MS), NFXL1 (Affinity Capture-RNA), NFXL1 (Affinity Capture-MS), NFXL1 (Affinity Capture-MS)

ESM2 similar proteins: A0JMY5, A2YEZ6, A3BDI8, A6QLA0, A9YUB1, B1AY10, D4A4T9, E0X9N4, O74853, P40798, P47226, P53971, Q09YN8, Q0D5B9, Q108U9, Q12986, Q15326, Q17QE2, Q18034, Q29RL2, Q2IBC3, Q2LAP6, Q2QLA1, Q2QLG8, Q4R7U2, Q54BK0, Q5PXT2, Q5R966, Q5RD91, Q5U2P3, Q5ZA07, Q5ZML4, Q67YE6, Q6DIR5, Q6IDS6, Q6NUA0, Q6ZNB6, Q7ZXE9, Q8N6M9, Q8R5C8

Diamond homologs: A0JMY5, A6QLA0, B1AY10, Q12986, Q18034, Q6ZNB6, Q9FFK8, Q9P2E3, Q9SY59, O74853, P40798, Q54BK0, P53971

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
Ub:E2	“up-regulates activity”	NFXL1	ubiquitination

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 140 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane	8	47.8×	5e-10
Transport of connexons to the plasma membrane	8	47.8×	5e-10
Gap junction trafficking and regulation	8	41.8×	8e-10
Gap junction trafficking	8	41.8×	8e-10
Post-chaperonin tubulin folding pathway	8	41.8×	8e-10
Metal ion SLC transporters	6	39.6×	1e-07
Formation of tubulin folding intermediates by CCT/TriC	8	37.2×	2e-09
Cooperation of Prefoldin and TriC/CCT in actin and tubulin folding	8	35.9×	2e-09

GO biological processes:

GO term	Partners	Fold	FDR
zinc ion transmembrane transport	6	33.7×	1e-05
intracellular zinc ion homeostasis	5	19.3×	7e-04
protein N-linked glycosylation	6	12.6×	8e-04
microtubule cytoskeleton organization	10	9.7×	3e-05
mitotic cell cycle	8	8.6×	7e-04

Disease & clinical

Clinical variants and AI predictions

ClinVar

72 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	61
Likely benign	2
Benign	4

Top pathogenic / likely-pathogenic (0)

SpliceAI

3243 predictions. Top by Δscore:

Variant	Effect	Δscore
4:47848333:CAGC:C	acceptor_gain	1.0000
4:47848335:GCC:G	acceptor_loss	1.0000
4:47848337:C:CC	acceptor_gain	1.0000
4:47848338:T:C	acceptor_loss	1.0000
4:47851090:GTTA:G	donor_loss	1.0000
4:47851091:TTA:T	donor_loss	1.0000
4:47851092:TA:T	donor_loss	1.0000
4:47851093:A:C	donor_loss	1.0000
4:47851094:C:CT	donor_loss	1.0000
4:47851144:TTTAT:T	acceptor_gain	1.0000
4:47851145:TTAT:T	acceptor_gain	1.0000
4:47851147:ATCTG:A	acceptor_loss	1.0000
4:47851149:C:CC	acceptor_gain	1.0000
4:47851149:CT:C	acceptor_loss	1.0000
4:47851153:T:C	acceptor_gain	1.0000
4:47851153:T:TC	acceptor_gain	1.0000
4:47851155:A:AC	acceptor_gain	1.0000
4:47851155:A:C	acceptor_gain	1.0000
4:47851158:CACA:C	acceptor_gain	1.0000
4:47851160:C:CT	acceptor_gain	1.0000
4:47851161:A:C	acceptor_gain	1.0000
4:47855161:AAGC:A	acceptor_loss	1.0000
4:47855162:AGC:A	acceptor_loss	1.0000
4:47855163:GC:G	acceptor_loss	1.0000
4:47855164:C:CA	acceptor_loss	1.0000
4:47862911:TTTTT:T	acceptor_gain	1.0000
4:47862912:TTTT:T	acceptor_gain	1.0000
4:47862913:TTT:T	acceptor_gain	1.0000
4:47862914:TT:T	acceptor_gain	1.0000
4:47862916:C:CC	acceptor_gain	1.0000

AlphaMissense

5990 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
4:47890634:A:G	C508R	1.000
4:47903267:C:A	W191C	1.000
4:47903267:C:G	W191C	1.000
4:47903269:A:G	W191R	1.000
4:47903269:A:T	W191R	1.000
4:47885927:C:G	C539S	0.999
4:47885928:A:G	C539R	0.999
4:47885928:A:T	C539S	0.999
4:47885985:A:G	C520R	0.999
4:47885994:A:G	C517R	0.999
4:47890619:G:C	H513D	0.999
4:47890621:C:G	C512S	0.999
4:47890622:A:G	C512R	0.999
4:47890622:A:T	C512S	0.999
4:47890632:A:C	C508W	0.999
4:47890633:C:G	C508S	0.999
4:47890634:A:T	C508S	0.999
4:47890638:A:C	H506Q	0.999
4:47890638:A:T	H506Q	0.999
4:47890648:C:G	C503S	0.999
4:47890649:A:G	C503R	0.999
4:47890649:A:T	C503S	0.999
4:47890667:A:G	C497R	0.999
4:47890679:A:G	C493R	0.999
4:47894191:A:G	C481R	0.999
4:47894206:A:G	C476R	0.999
4:47894224:A:G	C470R	0.999
4:47894236:A:G	C466R	0.999
4:47899376:G:C	H274D	0.999
4:47899379:A:G	C273R	0.999

dbSNP variants (sampled 300 via entrez): RS1000055505 (4:47901700 TA>T), RS1000071439 (4:47878840 T>C), RS1000088810 (4:47866792 T>C), RS1000130155 (4:47914757 G>C,T), RS1000242218 (4:47850611 C>A,T), RS1000290311 (4:47865014 C>A,T), RS1000316337 (4:47851005 C>A,T), RS1000341516 (4:47907607 A>G), RS1000345265 (4:47851674 G>C), RS1000347890 (4:47907995 G>A,C,T), RS1000377198 (4:47900608 G>A,C), RS1000436478 (4:47873766 T>C), RS1000447646 (4:47852046 T>C), RS1000489743 (4:47904949 G>A), RS1000500021 (4:47879314 G>A,T)

Disease associations

OMIM: gene MIM:620488 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067386 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.50	Kd	3185	nM	CHEMBL5653589
5.50	ED50	3185	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148866: Binding affinity to human NFXL1 incubated for 45 mins by Kinobead based pull down assay	kd	3.1851	uM

CTD chemical–gene interactions

45 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Cyclosporine	decreases methylation, increases expression, affects cotreatment	5
Valproic Acid	decreases expression, increases expression, affects expression	3
Air Pollutants	decreases expression, increases abundance, increases expression	2
Estradiol	increases expression	2
Tunicamycin	increases expression	2
Particulate Matter	decreases expression, increases abundance, increases expression	2
FR900359	increases phosphorylation	1
triphenyl phosphate	affects expression	1
propionaldehyde	increases methylation	1
pirinixic acid	increases expression, affects binding, increases activity	1
bisphenol A	decreases expression	1
nonanal	increases methylation	1
titanium dioxide	increases methylation	1
n-hexanal	increases methylation	1
sodium arsenite	decreases expression	1
butyraldehyde	increases methylation	1
perfluorooctanoic acid	increases expression	1
coumarin	decreases phosphorylation	1
caprylic aldehyde	increases methylation	1
pentanal	increases methylation	1
heptanal	increases methylation	1
nefazodone	affects cotreatment, increases expression	1
di-n-butylphosphoric acid	affects expression	1
perfluorooctane sulfonic acid	increases expression	1
pentabromodiphenyl ether	increases expression	1
jinfukang	affects cotreatment, decreases expression	1
Atazanavir Sulfate	affects cotreatment, increases expression	1
Resveratrol	affects cotreatment, increases expression	1
Sunitinib	increases expression	1
Carbamazepine	affects expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5651908	Binding	Binding affinity to human NFXL1 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.