NIT2

Summary

NIT2 (nitrilase family member 2, HGNC:29878) is a protein-coding gene on chromosome 3q12.2, encoding Omega-amidase NIT2 (Q9NQR4). Has omega-amidase activity.

Enables omega-amidase activity. Involved in asparagine metabolic process; glutamine metabolic process; and oxaloacetate metabolic process. Located in centrosome and cytosol.

Source: NCBI Gene 56954 — RefSeq curated summary.

At a glance

• GWAS associations: 2
• Clinical variants (ClinVar): 51 total
• Druggable target: yes
• MANE Select transcript: NM_020202

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:29878
Approved symbol	NIT2
Name	nitrilase family member 2
Location	3q12.2
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000114021
Ensembl biotype	protein_coding
OMIM	616769
Entrez	56954

Gene structure

Transcript identifiers

Ensembl transcripts: 11 — 6 protein_coding, 3 retained_intron, 2 nonsense_mediated_decay

ENST00000394140, ENST00000460317, ENST00000465368, ENST00000472392, ENST00000478856, ENST00000480073, ENST00000497785, ENST00000867929, ENST00000867930, ENST00000867931, ENST00000867932

RefSeq mRNA: 1 — MANE Select: NM_020202 NM_020202

CCDS: CCDS33806

Canonical transcript exons

ENST00000394140 — 10 exons

Exon	Start	End
ENSE00001227514	100355177	100361635
ENSE00001517603	100334757	100334798
ENSE00003571494	100348803	100348881
ENSE00003574715	100352404	100352502
ENSE00003575356	100341073	100341161
ENSE00003592881	100354772	100354827
ENSE00003633258	100339087	100339205
ENSE00003656199	100346181	100346255
ENSE00003670178	100339815	100339935
ENSE00003680812	100345585	100345678

Expression profiles

Bgee: expression breadth ubiquitous, 281 present calls, max score 97.98.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 33.4818 / max 151.3254, expressed in 1813 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
37605	32.3414	1811
37606	1.1404	721

Top tissues by expression

287 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
right lobe of liver	UBERON:0001114	97.98	gold quality
liver	UBERON:0002107	96.91	gold quality
mucosa of stomach	UBERON:0001199	96.90	gold quality
adult mammalian kidney	UBERON:0000082	96.41	gold quality
nephron tubule	UBERON:0001231	96.26	gold quality
kidney	UBERON:0002113	94.88	gold quality
skin of abdomen	UBERON:0001416	94.61	gold quality
gastrocnemius	UBERON:0001388	94.57	gold quality
skin of leg	UBERON:0001511	94.56	gold quality
adrenal tissue	UBERON:0018303	94.52	gold quality
cortex of kidney	UBERON:0001225	94.34	gold quality
muscle of leg	UBERON:0001383	94.32	gold quality
kidney epithelium	UBERON:0004819	94.31	gold quality
metanephros cortex	UBERON:0010533	94.22	gold quality
tibial nerve	UBERON:0001323	94.15	gold quality
gingival epithelium	UBERON:0001949	94.10	gold quality
zone of skin	UBERON:0000014	94.08	gold quality
apex of heart	UBERON:0002098	93.92	gold quality
subcutaneous adipose tissue	UBERON:0002190	93.90	gold quality
islet of Langerhans	UBERON:0000006	93.76	gold quality
hindlimb stylopod muscle	UBERON:0004252	93.50	gold quality
gingiva	UBERON:0001828	93.44	gold quality
right adrenal gland	UBERON:0001233	93.35	gold quality
esophagus mucosa	UBERON:0002469	93.34	gold quality
omental fat pad	UBERON:0010414	93.31	gold quality
right adrenal gland cortex	UBERON:0035827	93.31	gold quality
peritoneum	UBERON:0002358	93.28	gold quality
adipose tissue	UBERON:0001013	93.26	gold quality
adipose tissue of abdominal region	UBERON:0007808	93.25	gold quality
left lobe of thyroid gland	UBERON:0001120	93.23	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 3.

Experiment	Marker?	Max mean expression
E-HCAD-10	yes	32.62
E-ANND-3	yes	12.27
E-CURD-112	yes	9.28

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

20 targeting NIT2, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4476	100.00	68.18	2030
HSA-MIR-6876-5P	100.00	67.68	2126
HSA-MIR-186-5P	99.99	70.83	3707
HSA-MIR-4719	99.73	72.10	3329
HSA-MIR-891B	99.59	69.81	1083
HSA-MIR-766-3P	99.47	65.24	1811
HSA-MIR-3612	99.45	66.02	1333
HSA-MIR-650	99.45	65.77	1309
HSA-MIR-1206	99.30	69.32	1016
HSA-MIR-642A-3P	99.23	67.67	1258
HSA-MIR-642B-3P	99.23	67.67	1258
HSA-MIR-3925-5P	99.21	67.90	1466
HSA-MIR-4705	99.10	69.10	1091
HSA-MIR-589-5P	98.72	66.96	927
HSA-MIR-4733-3P	98.35	65.20	994
HSA-MIR-4443	98.02	66.25	1928
HSA-MIR-15B-3P	97.85	66.68	974
HSA-MIR-3162-5P	95.67	67.53	794
HSA-MIR-6800-5P	94.59	64.80	525
HSA-MIR-135A-3P	94.19	66.09	495

Literature-anchored findings (GeneRIF, showing 6)

• Genotype analysis in 4 types of tumor tissue showed allelic imbalance surrounding NIT2 locus; results show that NIT2 plays an important role in cell growth inhibition & links to malignancies, suggesting Nit2 may be a potential tumor suppressor candidate (PMID:17488281)
• The activity and substrate specificity of human Nit2 was determined with alpha-ketoglutaramate and succinamate as substrates. (PMID:22674578)
• Human NIT1/2 are not involved in the metabolism of vildagliptin. (PMID:25008847)
• Downregulation of NIT2 inhibits colon cancer cell proliferation and induces cell cycle arrest through the caspase-3 and PARP pathways (PMID:25738796)
• NIT2 overexpression predicts poor prognosis in tongue squamous cell carcinoma patients. (PMID:31925645)
• A novel efficient producer of human omega-amidase (Nit2) in Escherichia coli. (PMID:34391728)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	nit2	ENSDARG00000017590
mus_musculus	Nit2	ENSMUSG00000022751
rattus_norvegicus	Nit2	ENSRNOG00000027797
drosophila_melanogaster	CG8132	FBGN0037687

Paralogs (2): UPB1 (ENSG00000100024), NIT1 (ENSG00000158793)

Protein

Protein identifiers

Omega-amidase NIT2 — Q9NQR4 (reviewed: Q9NQR4)

Alternative names: Nitrilase homolog 2

All UniProt accessions (5): Q9NQR4, F8WF58, F8WF70, H7C579, V9HW91

UniProt curated annotations — full annotation on UniProt →

Function. Has omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting 2-oxoglutaramate and 2-oxosuccinamate to biologically useful 2-oxoglutarate and oxaloacetate, respectively.

Subunit / interactions. Homodimer.

Subcellular location. Cytoplasm.

Tissue specificity. Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.

Similarity. Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.

RefSeq proteins (1): NP_064587* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR003010	C-N_Hydrolase	Domain
IPR036526	C-N_Hydrolase_sf	Homologous_superfamily
IPR045254	Nit1/2_C-N_Hydrolase	Domain

Pfam: PF00795

Enzyme classification (BRENDA):

• EC 3.5.1.3 — omega-amidase (BRENDA: 20 organisms, 177 substrates, 34 inhibitors, 61 Km, 11 kcat entries)

Substrate kinetics (BRENDA)

23 substrates with measured Km, best-characterized 15. Km ranges are aggregated across organisms/conditions.

Substrate	Km (mM)	Measurements
2-OXOGLUTARAMATE	0.0054–9	10
HYDROXYLAMINE	1–100	8
SUCCINAMATE	0.14–17	6
GLUTARAMATE	1.27–25	5
2-OXOSUCCINAMATE	0.003–6.13	4
METHYL GLUTARATE	1.8–52	3
METHYLAMINE	30–55	3
ETHYL GLUTARATE	9.9–31	2
ETHYL SUCCINATE	8.3–17	2
METHYL SUCCINATE	0.7–8	2
PROPYL GLUTARATE	6.8–18	2
SUCCINATE	30–63	2
2-HYDROXYSUCCINAMATE	5.58	1
2-OXOGLUTARATE	3.3	1
GAMMA-ETHYL 2-OXOGLUTARATE	0.06	1

Catalyzed reactions (Rhea), 3 shown:

• a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH4(+) (RHEA:11716)
• 2-oxoglutaramate + H2O = 2-oxoglutarate + NH4(+) (RHEA:32963)
• 2-oxosuccinamate + H2O = oxaloacetate + NH4(+) (RHEA:59412)

UniProt features (15 total): modified residue 5, mutagenesis site 4, active site 3, chain 1, domain 1, sequence variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9NQR4-F1	97.62	0.98

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (3): 43 (proton acceptor); 112 (proton donor); 153 (nucleophile)

Post-translational modifications (5): 26, 68, 68, 123, 130

Mutagenesis-validated functional residues (4):

Position	Phenotype
43	loss of activity using succinamate as substrate.
112	loss of activity using succinamate as substrate.
116–128	less than 3% of wild-type activity using succinamate as substrate.
153	loss of activity using succinamate as substrate.

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-6798695	Neutrophil degranulation

MSigDB gene sets: 130 (showing top): REACTOME_INNATE_IMMUNE_SYSTEM, GOCC_SECRETORY_GRANULE, GOBP_ALPHA_AMINO_ACID_METABOLIC_PROCESS, GOBP_GLUTAMINE_FAMILY_AMINO_ACID_METABOLIC_PROCESS, GOBP_OXALOACETATE_METABOLIC_PROCESS, GOBP_DICARBOXYLIC_ACID_METABOLIC_PROCESS, GOBP_GLUTAMINE_METABOLIC_PROCESS, MARTINEZ_RB1_TARGETS_UP, RUTELLA_RESPONSE_TO_CSF2RB_AND_IL4_UP, RUTELLA_RESPONSE_TO_HGF_VS_CSF2RB_AND_IL4_DN, GOBP_ORGANIC_ACID_METABOLIC_PROCESS, MULLIGHAN_NPM1_SIGNATURE_3_DN, MARTINEZ_RB1_AND_TP53_TARGETS_UP, GRADE_COLON_AND_RECTAL_CANCER_UP, MULLIGHAN_MLL_SIGNATURE_2_DN

GO Biological Process (3): oxaloacetate metabolic process (GO:0006107), obsolete asparagine metabolic process (GO:0006528), L-glutamine metabolic process (GO:0006541)

GO Molecular Function (4): omega-amidase activity (GO:0050152), 2-oxoglutaramate amidase activity (GO:0106008), hydrolase activity (GO:0016787), hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides (GO:0016811)

GO Cellular Component (8): extracellular region (GO:0005576), mitochondrion (GO:0005739), centrosome (GO:0005813), cytosol (GO:0005829), specific granule lumen (GO:0035580), extracellular exosome (GO:0070062), tertiary granule lumen (GO:1904724), cytoplasm (GO:0005737)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
Innate Immune System	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
cytoplasm	2
dicarboxylic acid metabolic process	1
L-amino acid metabolic process	1
proteinogenic amino acid metabolic process	1
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	1
omega-amidase activity	1
catalytic activity	1
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	1
intracellular membrane-bounded organelle	1
centriole	1
microtubule organizing center	1
secretory granule lumen	1
specific granule	1
extracellular vesicle	1
intracellular organelle lumen	1
tertiary granule	1
intracellular anatomical structure	1

Protein interactions and networks

STRING

1588 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
NIT2	MDH2	P40926	490
NIT2	ISOC2	Q96AB3	487
NIT2	KYAT1	Q16773	484
NIT2	CIAO3	Q9H6Q4	463
NIT2	GLUL	P15104	449
NIT2	PKDCC	Q504Y2	428
NIT2	ISOC1	Q96CN7	417
NIT2	GPT2	Q8TD30	402
NIT2	GLUD1	P00367	401
NIT2	HARS1	P12081	400
NIT2	GULP1	Q9UBP9	398
NIT2	CDH8	P55286	387
NIT2	DMGDH	Q9UI17	383
NIT2	CINP	Q9BW66	381
NIT2	NOC2L	Q9Y3T9	377

IntAct

12 interactions, top by confidence:

A	B	Type	Score
LYRM4	NDUFAB1	psi-mi:“MI:0914”(association)	0.640
NIT2	PDIA4	psi-mi:“MI:0915”(physical association)	0.400
CFTR	NIT2	psi-mi:“MI:0915”(physical association)	0.370
CREB1	NFIX	psi-mi:“MI:0914”(association)	0.350
PRNP	WDR91	psi-mi:“MI:0914”(association)	0.350
COQ6	NDUFAB1	psi-mi:“MI:0914”(association)	0.350
rep	TMEM120B	psi-mi:“MI:0914”(association)	0.350
HLA-A	AIP	psi-mi:“MI:0914”(association)	0.350
AFG2B	MMP24OS	psi-mi:“MI:0914”(association)	0.350
NIT2		psi-mi:“MI:0915”(physical association)	0.000

BioGRID (89): LDHA (Co-fractionation), NIT2 (Co-fractionation), NIT2 (Co-fractionation), NIT2 (Co-fractionation), NIT2 (Co-fractionation), PARK7 (Co-fractionation), PRDX5 (Co-fractionation), PSMG4 (Co-fractionation), TPI1 (Co-fractionation), NIT2 (Affinity Capture-MS), NIT2 (Affinity Capture-MS), NIT2 (Affinity Capture-RNA), NIT2 (Co-fractionation), NIT2 (Co-fractionation), NIT2 (Co-fractionation)

ESM2 similar proteins: A0A140NCB4, A2R6M7, A2RA31, A4G3R1, B2JQY2, B6Q5I3, C6DKR8, G9AIU0, G9N4E3, O94660, P07874, P0DP64, P0DP66, P0DP68, P11444, P20960, P42593, P49954, P55175, P55176, P55178, P55357, P58054, P60327, P76008, Q0AJD9, Q216E0, Q221R3, Q2KXM7, Q3HVN1, Q44185, Q4VBV9, Q500U1, Q5S260, Q6INI7, Q6IR61, Q6N409, Q6RWG0, Q75SP7, Q82VS5

Diamond homologs: A0A140NCB4, A0A140NDS5, O31664, O76463, O76464, O94660, P0DP64, P0DP65, P0DP66, P0DP67, P55175, P55176, P55177, P55178, P58054, Q10166, Q28IE5, Q2T9R6, Q32LH4, Q47679, Q4VBV9, Q54JM9, Q557J5, Q5R4L6, Q6INI7, Q6IR61, Q75SP7, Q7TQ94, Q86X76, Q8RUF8, Q8VDK1, Q93NG1, Q94JV5, Q9JHW2, Q9NQR4, Q9UYV8, A6ZYQ3, B3LFZ1, B5VGI4, C4LYI2

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

51 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	32
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1644 predictions. Top by Δscore:

Variant	Effect	Δscore
3:100334794:GACCT:G	donor_gain	1.0000
3:100334796:CCT:C	donor_gain	1.0000
3:100334797:CT:C	donor_gain	1.0000
3:100334798:TGT:T	donor_loss	1.0000
3:100334799:G:GG	donor_gain	1.0000
3:100339083:TCAGC:T	acceptor_loss	1.0000
3:100339085:A:AG	acceptor_gain	1.0000
3:100339085:AGCTT:A	acceptor_loss	1.0000
3:100339086:G:GG	acceptor_gain	1.0000
3:100339086:GC:G	acceptor_gain	1.0000
3:100339086:GCT:G	acceptor_gain	1.0000
3:100339086:GCTTT:G	acceptor_gain	1.0000
3:100339202:GCCG:G	donor_gain	1.0000
3:100339203:CCGG:C	donor_loss	1.0000
3:100339206:G:C	donor_loss	1.0000
3:100339206:G:GG	donor_gain	1.0000
3:100339210:G:GG	donor_gain	1.0000
3:100339809:TTCTA:T	acceptor_loss	1.0000
3:100339810:TCTA:T	acceptor_loss	1.0000
3:100339811:CTAG:C	acceptor_loss	1.0000
3:100339812:TA:T	acceptor_loss	1.0000
3:100339813:A:T	acceptor_loss	1.0000
3:100339814:GGAAT:G	acceptor_gain	1.0000
3:100339899:G:GT	donor_gain	1.0000
3:100339911:G:GT	donor_gain	1.0000
3:100339912:A:T	donor_gain	1.0000
3:100341046:AT:A	acceptor_gain	1.0000
3:100341047:T:TA	acceptor_gain	1.0000
3:100341065:A:AG	acceptor_gain	1.0000
3:100341067:T:TA	acceptor_gain	1.0000

AlphaMissense

1781 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
3:100341160:A:C	K112T	0.997
3:100341161:G:C	K112N	0.997
3:100341161:G:T	K112N	0.997
3:100346222:T:C	F158L	0.997
3:100346224:T:A	F158L	0.997
3:100346224:T:G	F158L	0.997
3:100348832:T:C	F179L	0.997
3:100348834:T:A	F179L	0.997
3:100348834:T:G	F179L	0.997
3:100352483:A:C	S222R	0.997
3:100352485:C:A	S222R	0.997
3:100352485:C:G	S222R	0.997
3:100346207:T:C	C153R	0.996
3:100348881:G:C	R195P	0.996
3:100345594:T:C	F116L	0.995
3:100345596:T:A	F116L	0.995
3:100345596:T:G	F116L	0.995
3:100346208:G:A	C153Y	0.995
3:100346209:C:G	C153W	0.995
3:100341159:A:C	K112Q	0.994
3:100341160:A:T	K112M	0.994
3:100346214:A:T	D155V	0.994
3:100352406:C:A	A196D	0.994
3:100345631:A:T	E128V	0.993
3:100348812:T:C	L172P	0.993
3:100352430:C:A	A204D	0.993
3:100341157:G:T	R111I	0.992
3:100345632:A:C	E128D	0.992
3:100345632:A:T	E128D	0.992
3:100346213:G:C	D155H	0.992

dbSNP variants (sampled 300 via entrez): RS1000006047 (3:100361312 T>C), RS1000144310 (3:100361708 A>G,T), RS1000244018 (3:100335156 C>G,T), RS1000504748 (3:100354075 C>A), RS1000557352 (3:100347073 A>C), RS1000681261 (3:100360730 G>A), RS1000733108 (3:100360956 C>T), RS1000944250 (3:100334999 G>A), RS1000976662 (3:100335297 C>T), RS1000980951 (3:100354385 G>A,T), RS1001009368 (3:100359745 T>G), RS1001022879 (3:100347626 C>T), RS1001246667 (3:100340735 C>T), RS1001583289 (3:100353041 C>T), RS1001596087 (3:100339893 C>G,T)

Disease associations

OMIM: gene MIM:616769 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

Study	Trait	p-value
GCST009439_28	Age-related cognitive decline (language) (slope of z-scores)	6.000000e-07
GCST010002_434	Refractive error	5.000000e-25

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0007710	cognitive decline measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067107 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
8.58	Kd	2.637	nM	CHEMBL5653589
8.56	ED50	2.756	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148870: Binding affinity to human NIT2 incubated for 45 mins by Kinobead based pull down assay	kd	0.0026	uM

CTD chemical–gene interactions

40 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	decreases expression, increases expression	2
aristolochic acid I	decreases expression	1
bisphenol F	increases expression	1
TAK-243	increases sumoylation	1
dicrotophos	decreases expression	1
triphenyl phosphate	affects expression	1
trichostatin A	increases expression	1
arsenite	affects binding, increases reaction	1
microcystin RR	decreases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, increases expression	1
nutlin 3	affects cotreatment, increases secretion	1
ICG 001	increases expression	1
bisphenol B	increases expression	1
quinocetone	increases expression	1
Grape Seed Proanthocyanidins	affects cotreatment, decreases expression	1
bisphenol S	increases expression	1
jinfukang	affects cotreatment, increases expression	1
LDN 193189	increases expression, affects cotreatment	1
bisphenol AF	increases expression	1
Sunitinib	decreases expression	1
Acetaminophen	decreases expression	1
Air Pollutants	decreases expression, increases abundance	1
Atrazine	increases expression	1
Benzo(a)pyrene	affects methylation	1
Catechin	affects cotreatment, decreases expression	1
Cisplatin	affects cotreatment, increases expression	1
Dactinomycin	affects cotreatment, increases secretion	1
Dichlorodiphenyl Dichloroethylene	increases expression	1
Dexamethasone	increases expression	1
Doxorubicin	increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5651912	Binding	Binding affinity to human NIT2 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

1 cell lines: 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B3CE	Abcam HEK293T NIT2 KO	Transformed cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.