NLRP4

Summary

NLRP4 (NLR family pyrin domain containing 4, HGNC:22943) is a protein-coding gene on chromosome 19q13.43, encoding NACHT, LRR and PYD domains-containing protein 4 (Q96MN2). May be involved in inflammation and recognition of cytosolic pathogen-associated molecular patterns (PAMPs) not intercepted by membrane-bound receptors.

The protein encoded by this gene is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family, and is predicted to contain an N-terminal pyrin effector domain (PYD), a centrally-located nucleotide-binding and oligomerization domain (NACHT) and C-terminal leucine-rich repeats (LRR). This gene product has a demonstrated role as a negative regulator of autophagy and type I interferon signaling pathways as a result of protein interactions with its NACHT domain. The PYD domain has also been shown to be important in the inhibition of NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells).

Source: NCBI Gene 147945 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 176 total
• Druggable target: yes
• MANE Select transcript: NM_134444

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:22943
Approved symbol	NLRP4
Name	NLR family pyrin domain containing 4
Location	19q13.43
Locus type	gene with protein product
Status	Approved
Aliases	PYPAF4, FLJ32126, PAN2, RNH2, CLR19.5, CT58
Ensembl gene	ENSG00000160505
Ensembl biotype	protein_coding
OMIM	609645
Entrez	147945

Gene structure

Transcript identifiers

Ensembl transcripts: 4 — 4 protein_coding

ENST00000301295, ENST00000587464, ENST00000587891, ENST00000589437

RefSeq mRNA: 1 — MANE Select: NM_134444 NM_134444

CCDS: CCDS12936

Canonical transcript exons

ENST00000301295 — 10 exons

Exon	Start	End
ENSE00001052721	55870827	55870997
ENSE00001052724	55876996	55877166
ENSE00001115563	55861992	55862159
ENSE00001115570	55878794	55878964
ENSE00001115573	55857674	55859249
ENSE00001115576	55861386	55861547
ENSE00001115579	55867709	55867876
ENSE00001373388	55852016	55852360
ENSE00001714943	55836540	55836934
ENSE00002901863	55881470	55881855

Expression profiles

Bgee: expression breadth broad, 42 present calls, max score 100.00.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.0884 / max 73.3754, expressed in 27 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
177695	0.0687	20
208944	0.0116	6
177694	0.0081	1

Top tissues by expression

230 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
secondary oocyte	CL:0000655	100.00	gold quality
oocyte	CL:0000023	99.97	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	86.76	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	84.72	gold quality
sperm	CL:0000019	80.44	gold quality
kidney epithelium	UBERON:0004819	74.68	gold quality
epithelial cell of pancreas	CL:0000083	74.45	gold quality
testis	UBERON:0000473	67.65	gold quality
right testis	UBERON:0004534	67.22	gold quality
left testis	UBERON:0004533	66.73	gold quality
left ventricle myocardium	UBERON:0006566	56.68	gold quality
lymph node	UBERON:0000029	56.26	gold quality
cardiac muscle of right atrium	UBERON:0003379	54.94	gold quality
adult organism	UBERON:0007023	54.02	gold quality
upper arm skin	UBERON:0004263	53.52	gold quality
quadriceps femoris	UBERON:0001377	53.18	gold quality
vastus lateralis	UBERON:0001379	52.48	gold quality
buccal mucosa cell	CL:0002336	52.45	gold quality
pancreatic ductal cell	CL:0002079	52.17	silver quality
myocardium	UBERON:0002349	51.55	gold quality
vermiform appendix	UBERON:0001154	51.19	gold quality
tonsil	UBERON:0002372	51.10	gold quality
caecum	UBERON:0001153	48.68	gold quality
deltoid	UBERON:0001476	48.29	gold quality
layer of synovial tissue	UBERON:0007616	48.14	gold quality
tibialis anterior	UBERON:0001385	47.74	silver quality
inferior vagus X ganglion	UBERON:0005363	47.23	gold quality
ileal mucosa	UBERON:0000331	47.11	silver quality
tendon of biceps brachii	UBERON:0008188	47.06	gold quality
nasal cavity epithelium	UBERON:0005384	47.03	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-MTAB-3929	yes	1982.89
E-ANND-3	yes	3.60

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

31 targeting NLRP4, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-548AJ-3P	99.96	73.38	5345
HSA-MIR-548X-3P	99.96	73.38	5345
HSA-MIR-548J-3P	99.94	72.61	4881
HSA-MIR-548AE-3P	99.93	72.66	4867
HSA-MIR-548AH-3P	99.93	72.54	4872
HSA-MIR-548AM-3P	99.93	72.54	4872
HSA-MIR-548AQ-3P	99.93	72.66	4867
HSA-MIR-5680	99.91	69.83	3421
HSA-MIR-4493	99.90	66.48	977
HSA-MIR-6499-3P	99.90	66.38	1212
HSA-MIR-548D-3P	99.87	70.67	4362
HSA-MIR-548BB-3P	99.86	70.58	4354
HSA-MIR-548AC	99.84	70.77	4351
HSA-MIR-548H-3P	99.84	70.80	4349
HSA-MIR-548Z	99.84	70.80	4349
HSA-MIR-497-3P	99.61	69.71	1990
HSA-MIR-4762-5P	99.57	68.54	1424
HSA-MIR-4276	99.56	67.66	2514
HSA-MIR-6733-3P	99.54	67.80	1281
HSA-MIR-5190	99.15	67.76	1234
HSA-MIR-4664-5P	98.17	65.07	1020
HSA-MIR-1285-5P	98.01	68.71	779
HSA-MIR-6742-3P	97.95	64.50	1490
HSA-MIR-3665	97.73	65.08	975
HSA-MIR-3121-5P	97.30	66.62	1146
HSA-MIR-5194	96.77	63.91	1021
HSA-MIR-339-5P	96.73	66.01	820
HSA-MIR-6738-5P	96.33	63.61	815
HSA-MIR-6734-5P	95.70	65.56	950
HSA-MIR-1914-3P	95.07	63.37	762

Literature-anchored findings (GeneRIF, showing 11)

• NLRP4 is recruited to and accumulates in bacteria-containing phagosomes and transiently dissociates from beclin1 following group A streptococcus infection, thereby controlling the autophagic flux crucial for cell-autonomous antibacterial resistance. (PMID:21209283)
• Our results provide molecular insight into the mechanisms by which NLRP4-DTX4 targets TBK1 for degradation. (PMID:22388039)
• Structural and functional analysis of the NLRP4 pyrin domain. (PMID:22928810)
• Findings suggest that NLR family pyrin domain containing 2 (NLRP2P) is a processed pseudogene that regulates NF-kappaB RelA/p65 activity and thus represents the newest member of the POP family, pyrin-only protein 4 (POP4; NLRP4). (PMID:24871464)
• This study describes conformational stability of native NLRP14 and mutants in their monomeric and dimeric states and compares them to NLRP4, a representative of a native pyrin domain fold. (PMID:25403012)
• NLRP4 is an important mediator of cardiac remodeling in vitro and in vivo. (PMID:28486191)
• NLRP4 binds ARHGDIA (Rho GDP dissociation inhibitor alpha) to regulate Rho GTPase signaling and facilitate actin-mediated xenophagy. (PMID:29099277)
• The minor allele of rs16986718G > A in NLRP4 may be a genetic marker that predicts asthma exacerbation in adult asthmatics who smoke. (PMID:30526007)
• Somatic Mutation of NLRP Genes in Gastric and Colonic Cancers. (PMID:34257569)
• NLRP4 negatively regulates type I interferon response and influences the outcome in anti-programmed cell death protein (PD)-1/PD-ligand 1 therapy. (PMID:34927309)
• The hyper-expression of NLRP4 characterizes the occurrence of macrophage activation syndrome assessing STING pathway in adult-onset Still’s disease. (PMID:35467709)

Cross-species orthologs

10 orthologs

Organism	Symbol	Gene ID
mus_musculus	Nlrp4f	ENSMUSG00000032999
mus_musculus	Nlrp4b	ENSMUSG00000034087
mus_musculus	Nlrp4c	ENSMUSG00000034690
mus_musculus	Nlrp4a	ENSMUSG00000040601
mus_musculus	Nlrp4e	ENSMUSG00000045693
rattus_norvegicus	Nlrp4b	ENSRNOG00000015527
rattus_norvegicus	Nlrp4	ENSRNOG00000021996
rattus_norvegicus	Nlrp4f	ENSRNOG00000027705
rattus_norvegicus	Nlrp4a	ENSRNOG00000042030
rattus_norvegicus	Nlrp4al1	ENSRNOG00000059733

Paralogs (20): NLRP2 (ENSG00000022556), NLRP1 (ENSG00000091592), NOD1 (ENSG00000106100), NLRC5 (ENSG00000140853), NLRP12 (ENSG00000142405), NLRP14 (ENSG00000158077), NLRX1 (ENSG00000160703), NLRP3 (ENSG00000162711), NOD2 (ENSG00000167207), NLRP7 (ENSG00000167634), NLRC3 (ENSG00000167984), NLRP5 (ENSG00000171487), NLRP13 (ENSG00000173572), NLRP6 (ENSG00000174885), CIITA (ENSG00000179583), NLRP8 (ENSG00000179709), NLRP11 (ENSG00000179873), NLRP10 (ENSG00000182261), NLRP9 (ENSG00000185792), PYDC2 (ENSG00000253548)

Protein

Protein identifiers

NACHT, LRR and PYD domains-containing protein 4 — Q96MN2 (reviewed: Q96MN2)

Alternative names: Cancer/testis antigen 58, PAAD and NACHT-containing protein 2, PYRIN and NACHT-containing protein 2, PYRIN-containing APAF1-like protein 4, Ribonuclease inhibitor 2

All UniProt accessions (3): Q96MN2, K7ES09, K7ESK5

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in inflammation and recognition of cytosolic pathogen-associated molecular patterns (PAMPs) not intercepted by membrane-bound receptors. Acts as a negative regulator of the type I interferon signaling pathway by serving as an adapter to promote DTX4-mediated ubiquitination of activated TBK1, and its subsequent degradation. Suppresses NF-kappaB induction by the cytokines TNFA and IL1B, suggesting that it operates at a point of convergence in these two cytokine signaling pathways.

Subunit / interactions. Interacts with CHUK/IKKA, inhibiting its kinase activity.

Domain organisation. The pyrin domain is sufficient for suppression of NF-kappaB activity, it adopts a typical death domain fold, but in contrast to several NLRP family pyrin domains it doesn’t bind homotypically to the ASC adapter, which supports the observation that NLRP4 has no effect on IL1B activation.

Similarity. Belongs to the NLRP family.

Isoforms (3)

UniProt ID	Names	Canonical?
Q96MN2-1	1	yes
Q96MN2-2	2
Q96MN2-3	3

RefSeq proteins (1): NP_604393* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001611	Leu-rich_rpt	Repeat
IPR004020	DAPIN	Domain
IPR007111	NACHT_NTPase	Domain
IPR011029	DEATH-like_dom_sf	Homologous_superfamily
IPR027417	P-loop_NTPase	Homologous_superfamily
IPR032675	LRR_dom_sf	Homologous_superfamily
IPR041075	NOD1/2_WH	Domain
IPR041267	NLRP_HD2	Domain
IPR050637	NLRP_innate_immun_reg	Family

Pfam: PF02758, PF05729, PF13516, PF17776, PF17779

UniProt features (32 total): repeat 8, sequence conflict 7, sequence variant 6, helix 5, domain 2, splice variant 2, chain 1, binding site 1

Structure

Experimental structures (PDB)

1 structures.

PDB	Method	Resolution (Å)
4EWI	X-RAY DIFFRACTION	2.28

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q96MN2-F1	89.08	0.66

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (1): 155–162

Function

Pathways and Gene Ontology

Reactome pathways

8 pathways

ID	Pathway
R-HSA-1606341	IRF3 mediated activation of type 1 IFN
R-HSA-3134975	Regulation of innate immune responses to cytosolic DNA
R-HSA-3270619	IRF3-mediated induction of type I IFN
R-HSA-1606322	ZBP1(DAI) mediated induction of type I IFNs
R-HSA-168249	Innate Immune System
R-HSA-168256	Immune System
R-HSA-1834941	STING mediated induction of host immune responses
R-HSA-1834949	Cytosolic sensors of pathogen-associated DNA

MSigDB gene sets: 64 (showing top): REACTOME_INNATE_IMMUNE_SYSTEM, GOBP_INFLAMMATORY_RESPONSE, GOBP_NEGATIVE_REGULATION_OF_INNATE_IMMUNE_RESPONSE, GOBP_NEGATIVE_REGULATION_OF_RESPONSE_TO_BIOTIC_STIMULUS, GOBP_REGULATION_OF_IMMUNE_RESPONSE, GOBP_DEFENSE_RESPONSE_TO_OTHER_ORGANISM, GOBP_REGULATION_OF_RESPONSE_TO_STRESS, GOBP_NEGATIVE_REGULATION_OF_DEFENSE_RESPONSE, GOBP_REGULATION_OF_RESPONSE_TO_EXTERNAL_STIMULUS, AFFAR_YY1_TARGETS_DN, GOBP_REGULATION_OF_RESPONSE_TO_BIOTIC_STIMULUS, GOBP_REGULATION_OF_INFLAMMATORY_RESPONSE, GOBP_REGULATION_OF_DEFENSE_RESPONSE, GOBP_NEGATIVE_REGULATION_OF_IMMUNE_RESPONSE, GOBP_NEGATIVE_REGULATION_OF_RESPONSE_TO_EXTERNAL_STIMULUS

GO Biological Process (7): inflammatory response (GO:0006954), negative regulation of innate immune response (GO:0045824), regulation of inflammatory response (GO:0050727), proteasome-mediated ubiquitin-dependent protein catabolic process (GO:0043161), protein K48-linked ubiquitination (GO:0070936), antiviral innate immune response (GO:0140374), protein K33-linked deubiquitination (GO:1990168)

GO Molecular Function (4): ATP binding (GO:0005524), molecular adaptor activity (GO:0060090), nucleotide binding (GO:0000166), protein binding (GO:0005515)

GO Cellular Component (2): cytoplasm (GO:0005737), cytosol (GO:0005829)

Reactome top-level categories

Rollup of top-5 pathways:

Category	Pathways
Cytosolic sensors of pathogen-associated DNA	3
ZBP1(DAI) mediated induction of type I IFNs	1
STING mediated induction of host immune responses	1
Immune System	1
Innate Immune System	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
innate immune response	2
binding	2
cellular anatomical structure	2
defense response	1
negative regulation of response to biotic stimulus	1
negative regulation of defense response	1
negative regulation of response to external stimulus	1
regulation of innate immune response	1
negative regulation of immune response	1
inflammatory response	1
regulation of defense response	1
regulation of response to external stimulus	1
ubiquitin-dependent protein catabolic process	1
proteasomal protein catabolic process	1
protein polyubiquitination	1
defense response to virus	1
protein deubiquitination	1
adenyl ribonucleotide binding	1
purine ribonucleoside triphosphate binding	1
molecular_function	1
nucleoside phosphate binding	1
heterocyclic compound binding	1
intracellular anatomical structure	1
cytoplasm	1

Protein interactions and networks

STRING

619 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
NLRP4	BECN1	Q14457	986
NLRP4	DTX4	Q9Y2E6	983
NLRP4	AIM2	O14862	860
NLRP4	NLRP3	Q96P20	854
NLRP4	NLRP1	Q9C000	792
NLRP4	MEFV	O15553	761
NLRP4	NLRP12	P59046	758
NLRP4	NLRC4	Q9NPP4	754
NLRP4	PYCARD	Q9ULZ3	742
NLRP4	CASP1	P29466	714
NLRP4	NLRP7	Q8WX94	698
NLRP4	NLRP2	Q9NX02	691
NLRP4	TBK1	Q9UHD2	640
NLRP4	NLRP6	P59044	570
NLRP4	NOD1	Q9Y239	541

IntAct

4 interactions, top by confidence:

A	B	Type	Score
NLRP4	SUGT1	psi-mi:“MI:0915”(physical association)	0.400
NLRP4	PYDC2	psi-mi:“MI:0915”(physical association)	0.370
ATG16L1	ESYT2	psi-mi:“MI:0914”(association)	0.350

BioGRID (11): NLRP4 (Co-fractionation), NLRP4 (Affinity Capture-Western), NLRP4 (Affinity Capture-Western), NLRP4 (Affinity Capture-MS), NLRP4 (Affinity Capture-MS), NLRP4 (Affinity Capture-Western), TBK1 (Affinity Capture-Western), NLRP4 (Affinity Capture-Western), HSP90AA1 (Affinity Capture-Western), SUGT1 (Affinity Capture-Western), NLRP4 (Affinity Capture-Western)

ESM2 similar proteins: A1Z198, A6QLE5, B0FPE9, D4A523, D9I2F9, D9I2G1, D9I2G3, D9I2G4, D9I2H0, E9Q5G7, E9Q5R7, P59045, P59046, P59047, Q0GKD5, Q288C4, Q2LKU9, Q2LKV2, Q2LKV5, Q2LKW6, Q3TKR3, Q3UWY1, Q63035, Q647I9, Q66X01, Q66X03, Q66X05, Q66X19, Q66X22, Q6B966, Q7RTR0, Q7TPX8, Q86W24, Q86W25, Q86W26, Q86W28, Q8BU40, Q8BVP1, Q8C6J9, Q8CCN1

Diamond homologs: A0A2H5Q1B8, P59046, Q6Q4D0, Q96MN2, A0A386CAB9, A1Z198, A6QLE5, B0FPE9, D4A523, D9I2F9, D9I2G1, D9I2G3, D9I2G4, D9I2H0, E9Q5R7, O15553, P10775, P13489, P29315, P59044, P59045, Q0GKD5, Q288C4, Q2LKU9, Q2LKV2, Q2LKV5, Q2LKW6, Q3TKR3, Q63035, Q647I9, Q66X01, Q66X03, Q66X19, Q6B966, Q7RTR0, Q86W24, Q86W25, Q86W26, Q86W28, Q8BU40

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

176 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	134
Likely benign	16
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

1690 predictions. Top by Δscore:

Variant	Effect	Δscore
19:55859250:G:GG	donor_gain	1.0000
19:55861990:A:AG	acceptor_gain	1.0000
19:55861991:G:GG	acceptor_gain	1.0000
19:55861991:GA:G	acceptor_gain	1.0000
19:55861991:GAATA:G	acceptor_gain	1.0000
19:55862155:CTAGC:C	donor_gain	1.0000
19:55862158:GC:G	donor_gain	1.0000
19:55862160:G:GG	donor_gain	1.0000
19:55867877:G:GG	donor_gain	1.0000
19:55870823:ATAG:A	acceptor_gain	1.0000
19:55870825:A:AG	acceptor_gain	1.0000
19:55870825:AG:A	acceptor_gain	1.0000
19:55870825:AGGTT:A	acceptor_gain	1.0000
19:55870826:G:GG	acceptor_gain	1.0000
19:55870826:GG:G	acceptor_gain	1.0000
19:55870826:GGT:G	acceptor_gain	1.0000
19:55870826:GGTT:G	acceptor_gain	1.0000
19:55870826:GGTTG:G	acceptor_gain	1.0000
19:55870995:GTG:G	donor_gain	1.0000
19:55876994:AGTTT:A	acceptor_gain	1.0000
19:55876995:GTTTG:G	acceptor_gain	1.0000
19:55836731:G:GG	donor_gain	0.9900
19:55840899:A:AG	acceptor_gain	0.9900
19:55852107:T:TA	acceptor_gain	0.9900
19:55852350:G:GT	donor_gain	0.9900
19:55855425:A:T	donor_gain	0.9900
19:55855432:G:GT	donor_gain	0.9900
19:55857657:T:A	acceptor_gain	0.9900
19:55857664:A:AG	acceptor_gain	0.9900
19:55857664:ACT:A	acceptor_gain	0.9900

AlphaMissense

6602 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:55852161:A:C	K27N	0.996
19:55852161:A:T	K27N	0.996
19:55858741:T:C	F450L	0.989
19:55858742:T:C	F450S	0.989
19:55858743:C:A	F450L	0.989
19:55858743:C:G	F450L	0.989
19:55852133:T:C	L18P	0.987
19:55857937:T:C	F182L	0.986
19:55857939:C:A	F182L	0.986
19:55857939:C:G	F182L	0.986
19:55852169:T:C	L30P	0.985
19:55858601:C:A	A403D	0.985
19:55858633:T:C	F414L	0.985
19:55858635:T:A	F414L	0.985
19:55858635:T:G	F414L	0.985
19:55867789:T:C	L756P	0.985
19:55857875:A:T	K161I	0.984
19:55858076:T:C	F228S	0.984
19:55858634:T:C	F414S	0.984
19:55852157:T:C	F26S	0.983
19:55852160:A:T	K27I	0.983
19:55852133:T:A	L18H	0.982
19:55858761:G:C	Q456H	0.982
19:55858761:G:T	Q456H	0.982
19:55858768:T:C	C459R	0.982
19:55857856:G:A	G155R	0.981
19:55857856:G:C	G155R	0.981
19:55858507:T:G	Y372D	0.981
19:55870839:C:G	C789W	0.981
19:55852323:A:C	R81S	0.980

dbSNP variants (sampled 300 via entrez): RS1000002806 (19:55854826 G>A), RS1000045768 (19:55878635 C>A), RS1000056240 (19:55873316 G>A), RS1000104138 (19:55839938 C>T), RS1000109631 (19:55873033 T>C), RS1000237096 (19:55868778 T>C), RS1000241939 (19:55837339 G>A), RS1000340965 (19:55863752 G>A), RS1000412518 (19:55850779 G>A), RS1000456821 (19:55864029 A>G), RS1000587522 (19:55845891 T>C), RS1000640507 (19:55854572 A>G), RS1000643681 (19:55879850 T>A), RS1000682626 (19:55841154 T>A), RS1000713400 (19:55840967 G>A,T)

Disease associations

OMIM: gene MIM:609645 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST008369_18	Plasma anti-thyroglobulin levels	9.000000e-07

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL5465345 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: catalytic receptor — NOD-like receptor family

CTD chemical–gene interactions

5 total (human), top 5 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
propionaldehyde	decreases expression	1
CGP 52608	affects binding, increases reaction	1
abrine	increases expression	1
theaflavin-3,3’-digallate	affects expression	1
Permethrin	increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5374772	Binding	Inhibition of NLRP4 inflammasome activation in human PBMC assessed as reduction in IL-1beta secretion at 3 nM preincubated with LPS for 2.5 hrs followed by compound addition for 30 mins by ELISA	Discovery of Clinical Candidate NT-0796, a Brain-Penetrant and Highly Potent NLRP3 Inflammasome Inhibitor for Neuroinflammatory Disorders. — J Med Chem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.