NOX3

Summary

NOX3 (NADPH oxidase 3, HGNC:7890) is a protein-coding gene on chromosome 6q25.3, encoding NADPH oxidase 3 (Q9HBY0). NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor, upon formation of a complex with CYBA/p22phox.

This gene encodes a member of the NOX family of NADPH oxidases. These enzymes have the capacity to generate superoxide and other reactive oxygen species (ROS) and transport electrons across the plasma membrane. The ROS generated by family members have been implicated in numerous biological functions including host defense, posttranlational processing of proteins, cellular signaling, regulation of gene expression, and cell differentiation. The protein encoded by this gene is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity.

Source: NCBI Gene 50508 — RefSeq curated summary.

At a glance

• GWAS associations: 11
• Clinical variants (ClinVar): 100 total
• Druggable target: yes
• MANE Select transcript: NM_015718

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:7890
Approved symbol	NOX3
Name	NADPH oxidase 3
Location	6q25.3
Locus type	gene with protein product
Status	Approved
Aliases	GP91-3
Ensembl gene	ENSG00000074771
Ensembl biotype	protein_coding
OMIM	607105
Entrez	50508

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000159060

RefSeq mRNA: 1 — MANE Select: NM_015718 NM_015718

CCDS: CCDS5250

Canonical transcript exons

ENST00000159060 — 14 exons

Exon	Start	End
ENSE00000765456	155407130	155407254
ENSE00000765457	155411214	155411360
ENSE00000765458	155422694	155422856
ENSE00000765459	155428794	155429047
ENSE00000765460	155430843	155430935
ENSE00000765462	155436418	155436547
ENSE00000765463	155439956	155440137
ENSE00000765464	155443273	155443418
ENSE00000765467	155455034	155455129
ENSE00000813881	155395368	155395574
ENSE00000813882	155396809	155396962
ENSE00000813883	155453404	155453488
ENSE00000813884	155454811	155454921
ENSE00000813885	155455753	155455839

Expression profiles

Bgee: expression breadth tissue_specific, 4 present calls, max score 40.70.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.0881 / max 78.5640, expressed in 19 samples.

FANTOM5 promoters (5 alternative TSS)

Promoter ID	TPM avg	Samples expressed
76340	0.0539	9
76342	0.0226	7
76341	0.0048	1
76343	0.0045	2
76339	0.0024	1

Top tissues by expression

115 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
sural nerve	UBERON:0015488	40.70	gold quality
stromal cell of endometrium	CL:0002255	39.19	gold quality
primary visual cortex	UBERON:0002436	37.48	gold quality
colonic epithelium	UBERON:0000397	37.20	gold quality
ventricular zone	UBERON:0003053	36.48	gold quality
cortical plate	UBERON:0005343	36.47	gold quality
bone marrow cell	CL:0002092	36.16	gold quality
ganglionic eminence	UBERON:0004023	35.49	gold quality
prefrontal cortex	UBERON:0000451	33.84	gold quality
skeletal muscle tissue	UBERON:0001134	33.38	gold quality
superior frontal gyrus	UBERON:0002661	32.70	gold quality
muscle tissue	UBERON:0002385	32.27	gold quality
hindlimb stylopod muscle	UBERON:0004252	32.15	gold quality
bone marrow	UBERON:0002371	31.74	gold quality
frontal cortex	UBERON:0001870	31.06	silver quality
cerebral cortex	UBERON:0000956	31.01	silver quality
calcaneal tendon	UBERON:0003701	30.92	gold quality
Ammon’s horn	UBERON:0001954	30.69	silver quality
monocyte	CL:0000576	30.59	gold quality
leukocyte	CL:0000738	30.32	gold quality
anterior cingulate cortex	UBERON:0009835	29.16	gold quality
liver	UBERON:0002107	29.01	gold quality
urinary bladder	UBERON:0001255	28.77	gold quality
adenohypophysis	UBERON:0002196	28.21	gold quality
spleen	UBERON:0002106	28.15	gold quality
duodenum	UBERON:0002114	28.14	gold quality
smooth muscle tissue	UBERON:0001135	27.91	gold quality
lymph node	UBERON:0000029	27.57	gold quality
placenta	UBERON:0001987	27.56	gold quality
right frontal lobe	UBERON:0002810	27.12	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	3.76
E-HCAD-30	no	96.66

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

21 targeting NOX3, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-1277-5P	100.00	73.95	5056
HSA-MIR-205-3P	99.92	69.92	3165
HSA-MIR-30A-3P	99.87	69.74	2928
HSA-MIR-30D-3P	99.87	69.92	2917
HSA-MIR-30E-3P	99.87	69.68	2942
HSA-MIR-944	99.82	70.85	3042
HSA-MIR-4307	99.82	70.45	3374
HSA-MIR-33A-3P	99.70	70.27	3362
HSA-MIR-494-3P	99.70	71.45	2795
HSA-MIR-4677-5P	99.70	70.09	1940
HSA-MIR-545-5P	99.66	70.18	2308
HSA-MIR-7156-5P	99.64	68.81	1369
HSA-MIR-6739-3P	99.22	68.84	1843
HSA-MIR-367-5P	98.84	67.18	902
HSA-MIR-1267	98.24	69.05	837
HSA-MIR-6880-5P	98.08	65.59	1282
HSA-MIR-3116	97.07	65.78	1324
HSA-MIR-6726-5P	95.97	63.72	841
HSA-MIR-920	95.97	63.95	811
HSA-MIR-4300	95.85	64.56	1003
HSA-MIR-5591-5P	95.85	64.76	1002

Literature-anchored findings (GeneRIF, showing 13)

• Nox3 is regulated by NOXO1, p47phox, and p67phox (PMID:15181005)
• Nox3 functions together with p22(phox) as an enzyme constitutively producing superoxide (PMID:15824103)
• Rac1 regulates both oxidases Nox1 and Nox3 through the Nox activators (PMID:16507994)
• In conclusion, the results demonstrate that NOX3, a reactive oxygen species generating NADPH oxidase, plays an integral role in insulin-induced p42/44 Mitogen-activated protein kinase signal transmission and VEGF-A production. (PMID:16949073)
• Roles for p22phox at several distinct points in the maturation and assembly of a functionally competent Nox3 in the plasma membrane. (PMID:17140397)
• Potential candidate genes in these regions that have been implicated in diabetic nephropathy and/or renal damage in models of hypertension include CYBA (or P22PHOX) (16q24), NOX1 (10q22), and NOX3 (6q25.1-q26). (PMID:17336700)
• Down-regulation of NOX3 using siRNA prevented TNF-alpha-induced decrease of cellular glycogen. (PMID:20102709)
• palmitate-induced ROS generation and that NOX3-derived ROS may drive palmitate-induced hepatic insulin resistance through JNK and p38(MAPK) pathways. (PMID:20647313)
• Reduced matrigel invasion was mediated by reduced ROS levels coinciding with decreased expression of NADPH oxidase 2, 3, 4 and 5 involved in ROS production. (PMID:21901141)
• In lung transplants, our study highlights a donor-recipient interaction of NOX3 and NFE2L2 that increases the risk of PGD. (PMID:25439478)
• genetic variants in NOX3 may confer susceptibility to antithyroid drug-induced apoptosis of granulocytes. (PMID:28486791)
• The results suggested that rs6557421 variant in Nox3 and rs3744439 variant in Tbx4 might have potential effect on individual susceptibility to pulmonary hypertension. (PMID:30290780)
• Associations of noise kurtosis, genetic variations in NOX3 and lifestyle factors with noise-induced hearing loss. (PMID:32014026)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Nox3	ENSMUSG00000023802
rattus_norvegicus	Nox3	ENSRNOG00000016490

Paralogs (6): NOX1 (ENSG00000007952), NOX4 (ENSG00000086991), DUOX1 (ENSG00000137857), DUOX2 (ENSG00000140279), CYBB (ENSG00000165168), NOX5 (ENSG00000255346)

Protein

Protein identifiers

NADPH oxidase 3 — Q9HBY0 (reviewed: Q9HBY0)

Alternative names: Mitogenic oxidase 2, gp91phox homolog 3

All UniProt accessions (1): Q9HBY0

UniProt curated annotations — full annotation on UniProt →

Function. NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor, upon formation of a complex with CYBA/p22phox. Plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity.

Subunit / interactions. Interacts with CYBA/p22phox. Heterodimerization with CYBA/p22phox is essential for its activity and cell membrane localization.

Subcellular location. Cell membrane.

Post-translational modifications. N-glycosylated in a CYBA/p22phox-dependent manner.

Activity regulation. Activated by the ototoxic drug cisplatin. Activated by NOXO1. Cooperatively activated by NCF1 and NCF2 or NOXA1 in a phorbol 12-myristate 13-acetate (PMA)-dependent manner. Inhibited by diphenyleneiodonium chloride.

RefSeq proteins (1): NP_056533* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000778	Cyt_b245_heavy_chain	Family
IPR013112	FAD-bd_8	Domain
IPR013121	Fe_red_NAD-bd_6	Domain
IPR013130	Fe3_Rdtase_TM_dom	Domain
IPR017927	FAD-bd_FR_type	Domain
IPR017938	Riboflavin_synthase-like_b-brl	Homologous_superfamily
IPR039261	FNR_nucleotide-bd	Homologous_superfamily
IPR050369	RBOH/FRE	Family

Pfam: PF01794, PF08022, PF08030

Catalyzed reactions (Rhea), 1 shown:

• NADPH + 2 O2 = 2 superoxide + NADP(+) + H(+) (RHEA:63180)

UniProt features (21 total): topological domain 7, transmembrane region 6, domain 2, glycosylation site 2, chain 1, sequence variant 1, mutagenesis site 1, sequence conflict 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9HBY0-F1	89.84	0.66

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Glycosylation sites (2): 163, 238

Mutagenesis-validated functional residues (1):

Position	Phenotype
413	loss of catalytic activity.

Function

Pathways and Gene Ontology

Reactome pathways

3 pathways

ID	Pathway
R-HSA-5668599	RHO GTPases Activate NADPH Oxidases
R-HSA-9013149	RAC1 GTPase cycle
R-HSA-9013423	RAC3 GTPase cycle

MSigDB gene sets: 67 (showing top): RRAGTTGT_UNKNOWN, GOBP_SUPEROXIDE_METABOLIC_PROCESS, GOBP_RESPONSE_TO_GRAVITY, chr6q25, GOBP_EAR_DEVELOPMENT, NF1_Q6_01, GOBP_RESPONSE_TO_ABIOTIC_STIMULUS, TATA_C, AACTTT_UNKNOWN, GOBP_DETECTION_OF_ABIOTIC_STIMULUS, GOBP_DETECTION_OF_STIMULUS, GOBP_SUPEROXIDE_ANION_GENERATION, GOBP_MULTICELLULAR_ORGANISMAL_LEVEL_HOMEOSTASIS, GOMF_OXIDOREDUCTASE_ACTIVITY_ACTING_ON_NAD_P_H, GOBP_SENSORY_ORGAN_DEVELOPMENT

GO Biological Process (6): temperature homeostasis (GO:0001659), defense response (GO:0006952), detection of gravity (GO:0009590), superoxide anion generation (GO:0042554), otolith development (GO:0048840), response to gravity (GO:0009629)

GO Molecular Function (8): NAD(P)H oxidase H2O2-forming activity (GO:0016174), superoxide-generating NAD(P)H oxidase activity (GO:0016175), heme binding (GO:0020037), metal ion binding (GO:0046872), superoxide-generating NADPH oxidase activity (GO:0106292), protein binding (GO:0005515), oxidoreductase activity (GO:0016491), oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor (GO:0050664)

GO Cellular Component (5): cytoplasm (GO:0005737), plasma membrane (GO:0005886), NADPH oxidase complex (GO:0043020), extracellular exosome (GO:0070062), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
RHO GTPase cycle	2
RHO GTPase Effectors	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor	2
cellular anatomical structure	2
multicellular organismal-level homeostasis	1
response to stress	1
detection of external stimulus	1
detection of abiotic stimulus	1
response to gravity	1
superoxide metabolic process	1
inner ear development	1
anatomical structure development	1
response to abiotic stimulus	1
tetrapyrrole binding	1
cation binding	1
superoxide-generating NAD(P)H oxidase activity	1
binding	1
catalytic activity	1
oxidoreductase activity, acting on NAD(P)H	1
intracellular anatomical structure	1
membrane	1
cell periphery	1
plasma membrane protein complex	1
oxidoreductase complex	1
extracellular vesicle	1

Protein interactions and networks

STRING

902 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
NOX3	CYBA	P13498	996
NOX3	NOXO1	Q8NFA2	992
NOX3	NOX5	Q96PH1	986
NOX3	CYBB	P04839	982
NOX3	NOX4	Q9NPH5	982
NOX3	NOX1	Q9Y5S8	982
NOX3	DUOX1	Q9NRD9	981
NOX3	NOXA1	Q86UR1	971
NOX3	NCF2	P19878	964
NOX3	NCF1	P14598	956
NOX3	DUOX2	Q9NRD8	888
NOX3	NCF4	Q15080	856
NOX3	OC90	Q02509	784
NOX3	SH3PXD2B	A1X283	747
NOX3	SH3PXD2A	Q5TCZ1	738

IntAct

20 interactions, top by confidence:

A	B	Type	Score
CYBC1	NOX3	psi-mi:“MI:0915”(physical association)	0.560
BRAF	NOX3	psi-mi:“MI:2364”(proximity)	0.270
FBXW7	NOX3	psi-mi:“MI:2364”(proximity)	0.270
SMAD4	NOX3	psi-mi:“MI:2364”(proximity)	0.270
SPOP	NOX3	psi-mi:“MI:2364”(proximity)	0.270
NOX3	SPOP	psi-mi:“MI:2364”(proximity)	0.270
NOX3	EGFR	psi-mi:“MI:2364”(proximity)	0.270
NOX3	PTEN	psi-mi:“MI:2364”(proximity)	0.270
NOX3	PTPN11	psi-mi:“MI:2364”(proximity)	0.270
NOX3	TP53	psi-mi:“MI:2364”(proximity)	0.270
NOX3	AKT1	psi-mi:“MI:2364”(proximity)	0.270
CYBC1	NOX3	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (1): C17orf62 (Two-hybrid)

ESM2 similar proteins: A2AE42, A3A9H6, A3KPR5, A5D9A7, C4IYS8, O14569, P10897, P34465, P49447, Q3T130, Q497B2, Q4V8K1, Q503V1, Q53TN4, Q5CZL8, Q5E965, Q5RAJ4, Q5RCZ2, Q5RKJ2, Q5RKL5, Q5U2W7, Q60720, Q641Y1, Q658P3, Q67ZF6, Q687X5, Q6DDR3, Q6I681, Q6P1H1, Q6ZNA5, Q7XMK3, Q8CI59, Q8L856, Q8N8Q1, Q8NBI2, Q8VCZ2, Q91577, Q923B6, Q925G2, Q95204

Diamond homologs: O46522, O48538, O81210, O81211, P04839, P52649, Q2HXK9, Q2HXL0, Q5R5C5, Q5ZAJ0, Q61093, Q672J9, Q672K1, Q6J2K5, Q86GL4, Q8CIZ9, Q924V1, Q948T9, Q95L74, Q9FIJ0, Q9HBY0, Q9JHI8, Q9NPH5, Q9SBI0, Q9SW17, Q9WV87, Q9XYS3, Q9Y5S8, Q948U0, Q9NRD8, O81209, Q9NRD9, Q8CIY2, Q8HZK2, Q8HZK3, Q9MZF4, Q54F44, Q8VY13, Q8W110, Q96PH1

SIGNOR signaling

4 interactions.

A	Effect	B	Mechanism
NOXA1	“up-regulates activity”	NOX3	binding
NOX3	up-regulates	ROS
NOX3	“up-regulates quantity”	superoxide	“chemical modification”
NCF1	“up-regulates activity”	NOX3	binding

Disease & clinical

Clinical variants and AI predictions

ClinVar

100 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	89
Likely benign	4
Benign	1

Top pathogenic / likely-pathogenic (0)

SpliceAI

2285 predictions. Top by Δscore:

Variant	Effect	Δscore
6:155443271:A:AC	donor_gain	1.0000
6:155443272:C:CC	donor_gain	1.0000
6:155453487:CA:C	acceptor_gain	1.0000
6:155453489:C:CC	acceptor_gain	1.0000
6:155454926:CCACA:C	acceptor_gain	1.0000
6:155454927:CACA:C	acceptor_gain	1.0000
6:155454929:CA:C	acceptor_gain	1.0000
6:155454930:A:AC	acceptor_gain	1.0000
6:155454930:A:C	acceptor_gain	1.0000
6:155455028:ACTT:A	donor_loss	1.0000
6:155455029:CTTA:C	donor_loss	1.0000
6:155455032:A:AC	donor_gain	1.0000
6:155455032:A:AT	donor_loss	1.0000
6:155455032:ACAC:A	donor_gain	1.0000
6:155455032:ACACC:A	donor_gain	1.0000
6:155455033:C:CC	donor_gain	1.0000
6:155455033:CA:C	donor_gain	1.0000
6:155455033:CACC:C	donor_gain	1.0000
6:155455033:CACCC:C	donor_gain	1.0000
6:155455126:AGAGC:A	acceptor_loss	1.0000
6:155455127:GAGC:G	acceptor_loss	1.0000
6:155455128:AGCT:A	acceptor_loss	1.0000
6:155455129:GCTAG:G	acceptor_loss	1.0000
6:155455130:C:CC	acceptor_gain	1.0000
6:155455131:T:A	acceptor_loss	1.0000
6:155455749:TTA:T	donor_loss	1.0000
6:155455750:TA:T	donor_loss	1.0000
6:155455751:A:AC	donor_gain	1.0000
6:155455752:C:CG	donor_gain	1.0000
6:155455752:CT:C	donor_gain	1.0000

AlphaMissense

3717 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
6:155436436:A:C	F260L	0.982
6:155436436:A:T	F260L	0.982
6:155436438:A:G	F260L	0.982
6:155407170:A:G	W514R	0.981
6:155407170:A:T	W514R	0.981
6:155436437:A:G	F260S	0.975
6:155454902:C:G	R55P	0.973
6:155428933:G:C	H336D	0.968
6:155454899:G:T	A56E	0.968
6:155455123:A:G	W19R	0.967
6:155455123:A:T	W19R	0.967
6:155454874:G:C	N64K	0.966
6:155454874:G:T	N64K	0.966
6:155454884:A:G	L61P	0.966
6:155430926:A:G	W270R	0.964
6:155430926:A:T	W270R	0.964
6:155436437:A:C	F260C	0.964
6:155454909:A:G	W53R	0.962
6:155454909:A:T	W53R	0.962
6:155411348:A:G	W441R	0.961
6:155411348:A:T	W441R	0.961
6:155411238:A:C	F477L	0.960
6:155411238:A:T	F477L	0.960
6:155411240:A:G	F477L	0.960
6:155411318:A:G	W451R	0.960
6:155411318:A:T	W451R	0.960
6:155436469:C:A	W249C	0.960
6:155436469:C:G	W249C	0.960
6:155428925:G:C	F338L	0.959
6:155428925:G:T	F338L	0.959

dbSNP variants (sampled 300 via entrez): RS1000100450 (6:155419305 T>C,G), RS1000107379 (6:155444137 T>G), RS1000145454 (6:155430305 C>T), RS1000184232 (6:155433737 G>A), RS1000204584 (6:155419860 T>A,C), RS1000246185 (6:155413562 C>T), RS1000268721 (6:155451368 A>T), RS1000333444 (6:155449602 C>T), RS1000407278 (6:155439128 T>C), RS1000417974 (6:155445127 A>C,G), RS1000470519 (6:155395640 A>G,T), RS1000479326 (6:155425160 A>G), RS1000504898 (6:155401888 G>A,C,T), RS1000613270 (6:155457465 A>G), RS1000623817 (6:155440518 A>G)

Disease associations

OMIM: gene MIM:607105 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

11 associations (top):

Study	Trait	p-value
GCST003127_7	Lipoprotein (a) levels	1.000000e-09
GCST004749_107	Lung cancer in ever smokers	7.000000e-06
GCST005756_4	Dimensional psychopathology (Negative)	1.000000e-06
GCST005758_3	Dimensional psychopathology (Arousal)	9.000000e-07
GCST007297_18	Psychological distress	6.000000e-06
GCST008529_63	Tea consumption	2.000000e-06
GCST009391_214	Metabolite levels	4.000000e-06
GCST010135_22	Oily fish consumption	8.000000e-10
GCST010140_14	Pork consumption	8.000000e-10
GCST010988_381	Adult body size	5.000000e-09
GCST90000582_16	Spontaneous coronary artery dissection	1.000000e-06

EFO canonical traits (6, from GWAS)

EFO ID	Trait name
EFO:0006925	lipoprotein A measurement
EFO:0009096	negative domain measurement
EFO:0009099	arousal domain measurement
EFO:0007006	depressive symptom measurement
EFO:0010091	tea consumption measurement
EFO:0008111	diet measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL1741216 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

PharmGKB variants

2 variants.

Variant	Genes	Level	Score	#Clin annots	Drugs
rs200245496	NOX3		0.00	0
rs117412760	NOX3		0.00	0

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: enzyme — NADPH oxidases

ChEMBL bioactivities

6 potent at pChembl≥5 of 7 total, top 5 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.90	IC50	1250	nM	CHEMBL5220686
5.52	IC50	3000	nM	CHEMBL407734
5.50	IC50	3200	nM	CHEMBL5218591
5.50	IC50	3200	nM	CHEMBL5398181
5.12	IC50	7500	nM	CHEMBL541018

PubChem BioAssay actives

4 with measured affinity, of 5 total; 4 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
2-N-(3,4-dimethylphenyl)-6-[[4-(3-methoxyphenyl)piperazin-1-yl]methyl]-1,3,5-triazine-2,4-diamine	1917071: Inhibition of human NOX3	ic50	1.2500	uM
1-(10H-phenothiazin-2-yl)ethanone	2013297: Inhibition of human NOX3 transfected in HEK293T cells incubated for 16 hrs ans measured after 30 mins by luminol-chemiluminescence based assay	ic50	3.0000	uM
2-N-(3,4-dimethylphenyl)-6-[[4-(4-methylphenyl)piperazin-1-yl]methyl]-1,3,5-triazine-2,4-diamine	2013309: Inhibition of NOX3 (unknown origin)	ic50	3.2000	uM
2-N-(3,4-dimethylphenyl)-6-[4-(4-methylphenyl)piperazin-1-yl]-1,3,5-triazine-2,4-diamine	1917071: Inhibition of human NOX3	ic50	3.2000	uM

CTD chemical–gene interactions

12 total (human), top 12 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
ethyl-p-hydroxybenzoate	increases expression	1
perfluorooctanoic acid	increases expression	1
chromium hexavalent ion	increases expression	1
perfluorooctane sulfonic acid	increases expression	1
CGP 52608	affects binding, increases reaction	1
perfluoro-n-nonanoic acid	increases expression	1
Benzo(a)pyrene	affects methylation, decreases methylation	1
Progesterone	decreases expression	1
Silicon Dioxide	decreases expression	1
Tretinoin	decreases expression	1
Aflatoxin B1	increases methylation	1
Palmitic Acid	increases expression	1

ChEMBL screening assays

5 unique, capped per target: 4 binding, 1 unclassified

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL1738360	Unclassified	PUBCHEM_BIOASSAY: Late stage results from the probe development effort to identify inhibitors of (NADPH oxidase 1) NOX1: Family selectivity: Set 2. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID1792, AID1796,	PubChem BioAssay data set
CHEMBL5216593	Binding	Inhibition of human NOX3	A closer look at N2,6-substituted 1,3,5-triazine-2,4-diamines: Advances in synthesis and biological activities. — Eur J Med Chem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.