NUDT4

Summary

NUDT4 (nudix hydrolase 4, HGNC:8051) is a protein-coding gene on chromosome 12q22, encoding Diphosphoinositol polyphosphate phosphohydrolase 2 (Q9NZJ9). Cleaves the beta-phosphate from diphosphoinositol polyphosphates such as PP-InsP5 (diphosphoinositol pentakisphosphate), PP-InsP4 (diphosphoinositol tetrakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduc….

The protein encoded by this gene regulates the turnover of diphosphoinositol polyphosphates. The turnover of these high-energy diphosphoinositol polyphosphates represents a molecular switching activity with important regulatory consequences. Molecular switching by diphosphoinositol polyphosphates may contribute to regulating intracellular trafficking. Several alternatively spliced transcript variants have been described, but the full-length nature of some variants has not been determined. Isoforms DIPP2alpha and DIPP2beta are distinguishable from each other solely by DIPP2beta possessing one additional amino acid due to intron boundary skidding in alternate splicing.

Source: NCBI Gene 11163 — RefSeq curated summary.

At a glance

• GWAS associations: 2
• Clinical variants (ClinVar): 16 total
• Druggable target: yes
• MANE Select transcript: NM_019094

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:8051
Approved symbol	NUDT4
Name	nudix hydrolase 4
Location	12q22
Locus type	gene with protein product
Status	Approved
Aliases	DIPP2, HDCMB47P, KIAA0487, DIPP2alpha, DIPP2beta
Ensembl gene	ENSG00000173598
Ensembl biotype	protein_coding
OMIM	609229
Entrez	11163

Gene structure

Transcript identifiers

Ensembl transcripts: 12 — 12 protein_coding

ENST00000337179, ENST00000415493, ENST00000546925, ENST00000547014, ENST00000548662, ENST00000549992, ENST00000550056, ENST00000884346, ENST00000884347, ENST00000884348, ENST00000913352, ENST00000945061

RefSeq mRNA: 5 — MANE Select: NM_019094 NM_001301022, NM_001301023, NM_001301024, NM_019094, NM_199040

CCDS: CCDS44952, CCDS73504, CCDS76584, CCDS9044

Canonical transcript exons

ENST00000415493 — 5 exons

Exon	Start	End
ENSE00001197938	93395489	93395533
ENSE00001416265	93377925	93378421
ENSE00001601742	93398771	93398855
ENSE00003655199	93394609	93394719
ENSE00003742280	93399177	93408146

Expression profiles

Bgee: expression breadth ubiquitous, 292 present calls, max score 99.51.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 35.0393 / max 551.0308, expressed in 1816 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
127346	31.9388	1810
127347	2.5878	1183
127348	0.5127	227

Top tissues by expression

295 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
heart right ventricle	UBERON:0002080	99.51	gold quality
parotid gland	UBERON:0001831	99.24	gold quality
left ventricle myocardium	UBERON:0006566	98.75	gold quality
renal medulla	UBERON:0000362	98.74	gold quality
myocardium	UBERON:0002349	98.50	gold quality
caput epididymis	UBERON:0004358	98.34	gold quality
corpus epididymis	UBERON:0004359	98.10	gold quality
cardiac muscle of right atrium	UBERON:0003379	97.96	gold quality
gall bladder	UBERON:0002110	97.94	gold quality
cardiac ventricle	UBERON:0002082	97.79	gold quality
trabecular bone tissue	UBERON:0002483	97.78	gold quality
heart left ventricle	UBERON:0002084	97.74	gold quality
mucosa of sigmoid colon	UBERON:0004993	97.48	gold quality
urethra	UBERON:0000057	97.36	gold quality
heart	UBERON:0000948	97.31	gold quality
colonic mucosa	UBERON:0000317	97.30	gold quality
left ovary	UBERON:0002119	97.27	gold quality
right ovary	UBERON:0002118	97.23	gold quality
choroid plexus epithelium	UBERON:0003911	97.20	gold quality
colonic epithelium	UBERON:0000397	97.16	gold quality
gluteal muscle	UBERON:0002000	97.16	gold quality
ganglionic eminence	UBERON:0004023	97.14	gold quality
secondary oocyte	CL:0000655	97.12	gold quality
bronchial epithelial cell	CL:0002328	97.05	gold quality
buccal mucosa cell	CL:0002336	96.96	gold quality
cauda epididymis	UBERON:0004360	96.75	gold quality
cardiac atrium	UBERON:0002081	96.71	gold quality
ventricular zone	UBERON:0003053	96.67	gold quality
adrenal tissue	UBERON:0018303	96.66	gold quality
left coronary artery	UBERON:0001626	96.64	gold quality

Single-cell (SCXA)

Detected in 11 experiment(s), a significant marker in 10.

Experiment	Marker?	Max mean expression
E-MTAB-6819	yes	886.92
E-MTAB-10287	yes	49.04
E-GEOD-137537	yes	29.76
E-MTAB-7316	yes	28.20
E-MTAB-9221	yes	16.53
E-MTAB-9388	yes	11.09
E-MTAB-5061	yes	9.40
E-GEOD-134144	yes	8.50
E-HCAD-9	yes	6.72
E-MTAB-6108	no	373.87
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

207 targeting NUDT4, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-LET-7A-3P	100.00	74.03	3932
HSA-LET-7B-3P	100.00	74.08	3913
HSA-LET-7F-1-3P	100.00	74.02	3928
HSA-MIR-98-3P	100.00	74.08	3907
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-518D-5P	100.00	67.51	979
HSA-MIR-518E-5P	100.00	67.66	954
HSA-MIR-518F-5P	100.00	67.51	979
HSA-MIR-519A-5P	100.00	67.66	954
HSA-MIR-519B-5P	100.00	67.66	954
HSA-MIR-519C-5P	100.00	67.66	954
HSA-MIR-520C-5P	100.00	67.51	979
HSA-MIR-522-5P	100.00	67.66	954
HSA-MIR-523-5P	100.00	67.66	954
HSA-MIR-526A-5P	100.00	67.51	979
HSA-MIR-200B-3P	100.00	73.31	2693
HSA-MIR-200C-3P	100.00	73.35	2685
HSA-MIR-429	100.00	73.44	2698
HSA-MIR-6867-5P	100.00	82.21	3464
HSA-MIR-5692A	100.00	74.40	6850
HSA-MIR-4682	100.00	68.89	1258
HSA-MIR-340-5P	100.00	72.50	4437
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-3662	99.99	73.82	5684
HSA-MIR-371B-5P	99.99	75.34	4759
HSA-MIR-4282	99.99	75.36	6408
HSA-LET-7F-2-3P	99.98	70.98	2588
HSA-MIR-1185-1-3P	99.98	71.04	2593
HSA-MIR-1185-2-3P	99.98	71.04	2593

Literature-anchored findings (GeneRIF, showing 1)

• This paper demonstrates that KIAA0487 (AB007956) is the 3’-UTR for human diphosphoinositol polyphosphate phosphohydrolase type II (PMID:10777568)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	nudt4b	ENSDARG00000045878
danio_rerio	nudt4a	ENSDARG00000057767
mus_musculus	Nudt4	ENSMUSG00000020029
rattus_norvegicus	Nudt4	ENSRNOG00000009094
drosophila_melanogaster	Aps	FBGN0036111

Paralogs (4): NUDT10 (ENSG00000122824), NUDT4B (ENSG00000177144), NUDT11 (ENSG00000196368), NUDT3 (ENSG00000272325)

Protein

Protein identifiers

Diphosphoinositol polyphosphate phosphohydrolase 2 — Q9NZJ9 (reviewed: Q9NZJ9)

Alternative names: Diadenosine 5’,5’’’-P1,P6-hexaphosphate hydrolase 2, Nucleoside diphosphate-linked moiety X motif 4

All UniProt accessions (4): Q9NZJ9, A0A0C4DGJ4, F8VRL4, F8VRR0

UniProt curated annotations — full annotation on UniProt →

Function. Cleaves the beta-phosphate from diphosphoinositol polyphosphates such as PP-InsP5 (diphosphoinositol pentakisphosphate), PP-InsP4 (diphosphoinositol tetrakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction. Diadenosine polyphosphates, particularly Ap6A (P(1),P(6)-bis(5a-adenosyl) hexaphosphate) and Ap5A (P(1),P(5)-bis(5’-adenosyl) pentaphosphate) are downstream effectors of a signaling cascade that regulates cardiac KATP channels, can also be substrates, although with lower preference than the diphosphoinositol polyphosphates. Can also catalyze the hydrolysis of 5-phosphoribose 1-diphosphate, generating the glycolytic activator ribose 1,5-bisphosphate. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.

Subcellular location. Cytoplasm.

Tissue specificity. Expressed in heart and, at lower level in skeletal muscle, pancreas and kidney.

Cofactor. Binds 3 Mg(2+) or Mn(2+) ions per subunit.

Miscellaneous. the additional CAG codon in the isoform 2 (DIPP2beta) mRNA introduces a single, in-frame amino acid residue (Gln86) into the protein. This insertion engenders the DIPP2beta protein with 2- to 5-fold lower catalytic activity than isoform 1 (DIPP2alpha) for the diphosphoinositol polyphosphates and Ap6A, while granting activity towards the Ap5A.

Similarity. Belongs to the Nudix hydrolase family. DIPP subfamily.

Isoforms (3)

UniProt ID	Names	Canonical?
Q9NZJ9-1	1, Alpha, DIPP2alpha	yes
Q9NZJ9-2	2, Beta, DIPP2beta
Q9NZJ9-3	3

RefSeq proteins (5): NP_001287951, NP_001287952, NP_001287953, NP_061967, NP_950241 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000086	NUDIX_hydrolase_dom	Domain
IPR015797	NUDIX_hydrolase-like_dom_sf	Homologous_superfamily
IPR020084	NUDIX_hydrolase_CS	Conserved_site
IPR047198	DDP-like_NUDIX	Domain

Pfam: PF00293

Catalyzed reactions (Rhea), 6 shown:

• 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + H2O = 1D-myo-inositol hexakisphosphate + phosphate + H(+) (RHEA:22384)
• P(1),P(5)-bis(5’-adenosyl) pentaphosphate + H2O = ADP + ATP + 2 H(+) (RHEA:30527)
• P(1),P(6)-bis(5’-adenosyl) hexaphosphate + H2O = 2 ATP + 2 H(+) (RHEA:32043)
• 3,5-bis(diphospho)-1D-myo-inositol 1,2,4,6-tetrakisphosphate + H2O = 3-diphospho-1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate + 2 H(+) (RHEA:56312)
• 5-diphospho-1D-myo-inositol 1,3,4,6-tetrakisphosphate + H2O = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + phosphate + H(+) (RHEA:59500)
• 5-phospho-alpha-D-ribose 1-diphosphate + H2O = alpha-D-ribose 1,5-bisphosphate + phosphate + H(+) (RHEA:80811)

UniProt features (32 total): binding site 10, strand 6, sequence conflict 5, helix 3, splice variant 2, chain 1, domain 1, modified residue 1, short sequence motif 1, turn 1, active site 1

Structure

Experimental structures (PDB)

2 structures.

PDB	Method	Resolution (Å)
7NNJ	X-RAY DIFFRACTION	1.75
5LTU	X-RAY DIFFRACTION	2.23

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9NZJ9-F1	86.25	0.76

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (1): 69 (proton acceptor)

Ligand- & substrate-binding residues (10): 70; 89–91; 115; 133; 10; 18–20; 39–41; 50; 66; 66

Post-translational modifications (1): 1

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-1855167	Synthesis of pyrophosphates in the cytosol

MSigDB gene sets: 285 (showing top): GSE45365_NK_CELL_VS_CD8_TCELL_DN, GSE18804_SPLEEN_MACROPHAGE_VS_COLON_TUMORAL_MACROPHAGE_DN, GOBP_INOSITOL_PHOSPHATE_METABOLIC_PROCESS, AAGCCAT_MIR135A_MIR135B, GOBP_POLYOL_METABOLIC_PROCESS, GCM_ZNF198, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_DN, DITTMER_PTHLH_TARGETS_UP, GOBP_CARBOHYDRATE_DERIVATIVE_CATABOLIC_PROCESS, IVANOVA_HEMATOPOIESIS_MATURE_CELL, GCM_PPM1D, GOBP_ORGANOPHOSPHATE_METABOLIC_PROCESS, CTATGCA_MIR153, GOBP_CARBOHYDRATE_DERIVATIVE_METABOLIC_PROCESS, RODWELL_AGING_KIDNEY_NO_BLOOD_DN

GO Biological Process (6): calcium-mediated signaling (GO:0019722), intracellular signal transduction (GO:0035556), diphosphoinositol polyphosphate metabolic process (GO:0071543), diadenosine pentaphosphate catabolic process (GO:1901907), diadenosine hexaphosphate catabolic process (GO:1901909), adenosine 5’-(hexahydrogen pentaphosphate) catabolic process (GO:1901911)

GO Molecular Function (13): endopolyphosphatase activity (GO:0000298), diphosphoinositol-polyphosphate diphosphatase activity (GO:0008486), snoRNA binding (GO:0030515), bis(5’-adenosyl)-hexaphosphatase activity (GO:0034431), bis(5’-adenosyl)-pentaphosphatase activity (GO:0034432), metal ion binding (GO:0046872), inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity (GO:0052845), inositol-3,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity (GO:0052848), inositol-5-diphosphate-1,3,4,6-tetrakisphosphate diphosphatase activity (GO:0106211), RNA binding (GO:0003723), protein binding (GO:0005515), pyrophosphatase activity (GO:0016462), hydrolase activity (GO:0016787)

GO Cellular Component (3): nucleus (GO:0005634), cytoplasm (GO:0005737), cytosol (GO:0005829)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
Inositol phosphate metabolism	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
pyrophosphatase activity	4
intracellular anatomical structure	2
diadenosine polyphosphate catabolic process	2
cellular anatomical structure	2
intracellular signaling cassette	1
signal transduction	1
inositol phosphate metabolic process	1
purine ribonucleotide catabolic process	1
RNA binding	1
cation binding	1
inositol diphosphate pentakisphosphate diphosphatase activity	1
inositol bisdiphosphate tetrakisphosphate diphosphatase activity	1
inositol diphosphate tetrakisphosphate diphosphatase activity	1
nucleic acid binding	1
binding	1
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	1
catalytic activity	1
intracellular membrane-bounded organelle	1
cytoplasm	1

Protein interactions and networks

STRING

0 interactions, top by confidence (×1000):

IntAct

23 interactions, top by confidence:

A	B	Type	Score
NUDT4	KEAP1	psi-mi:“MI:0915”(physical association)	0.560
KEAP1	NUDT4	psi-mi:“MI:0915”(physical association)	0.560
NUDT4	HSPB1	psi-mi:“MI:0915”(physical association)	0.560
NUDT4	WFS1	psi-mi:“MI:0915”(physical association)	0.560
HTT	NUDT4	psi-mi:“MI:0915”(physical association)	0.560
	TK2	psi-mi:“MI:0915”(physical association)	0.400
NUDT4	PCNA	psi-mi:“MI:0915”(physical association)	0.370
DISC1	AGRN	psi-mi:“MI:0914”(association)	0.350
	AGPS	psi-mi:“MI:0914”(association)	0.350
SLC6A2	CLGN	psi-mi:“MI:0914”(association)	0.350
SWSAP1	NACA	psi-mi:“MI:2364”(proximity)	0.270
SF3B4	MED19	psi-mi:“MI:2364”(proximity)	0.270

BioGRID (19): NUDT4 (Two-hybrid), NUDT4 (Affinity Capture-MS), NUDT4 (Affinity Capture-MS), NUDT4 (Affinity Capture-MS), NUDT4 (Affinity Capture-MS), NUDT4 (Positive Genetic), NUDT4 (Affinity Capture-MS), NUDT4 (Affinity Capture-MS), NUDT4 (Affinity Capture-MS), NUDT4 (Proximity Label-MS), NUDT4 (Affinity Capture-MS), NUDT4 (Proximity Label-MS), NUDT4 (Proximity Label-MS), NUDT4 (Proximity Label-MS), NUDT4 (Affinity Capture-MS)

ESM2 similar proteins: A0A024RBG1, A2VE79, A3KMI0, A7E320, B0R160, B6CHA3, F4JLK2, F6UA42, O22951, O45830, O59761, O95989, P0C027, P0C028, P32271, Q08C92, Q09790, Q566C7, Q58CW0, Q5RAF0, Q5RDX4, Q5U243, Q6NPD7, Q6P5D3, Q7TMI3, Q7TPK1, Q7YTB0, Q8BJM7, Q8CIG3, Q8L7W2, Q8NB78, Q8NFP7, Q8R2U6, Q8VDF2, Q8VHT6, Q91WU5, Q96G61, Q96PU4, Q96T88, Q99321

Diamond homologs: A0A024RBG1, A2VE79, O95989, P0C027, P0C028, P53370, P70563, P96590, Q09790, Q52K88, Q566C7, Q58CW0, Q5RAF0, Q8CH40, Q8NFP7, Q8R2U6, Q8VY81, Q93ZY7, Q96G61, Q99321, Q99MY2, Q9JI46, Q9LE73, Q9LHK1, Q9LQU5, Q9NZJ9, Q9ZU95, Q9RVK2, P32091, P59659, A1AA28, A1WVE9, A6W1S0, A7ZKS4, A7ZZ89, B0RN07, B1IUD3, B1LI09, B1XA44, B2FJU2

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

16 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	16
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

873 predictions. Top by Δscore:

Variant	Effect	Δscore
12:93378413:G:GT	donor_gain	1.0000
12:93378416:G:GT	donor_gain	1.0000
12:93378419:GAGG:G	donor_loss	1.0000
12:93378420:AGGT:A	donor_loss	1.0000
12:93378421:GGT:G	donor_loss	1.0000
12:93378422:G:C	donor_loss	1.0000
12:93378423:T:G	donor_loss	1.0000
12:93394606:CAGG:C	acceptor_loss	1.0000
12:93394607:A:AG	acceptor_gain	1.0000
12:93394607:AGG:A	acceptor_loss	1.0000
12:93394608:G:GG	acceptor_gain	1.0000
12:93394608:GGT:G	acceptor_gain	1.0000
12:93394705:G:GT	donor_gain	1.0000
12:93394705:G:T	donor_gain	1.0000
12:93394716:GGAG:G	donor_gain	1.0000
12:93394717:GAG:G	donor_gain	1.0000
12:93394717:GAGG:G	donor_gain	1.0000
12:93394718:AGG:A	donor_loss	1.0000
12:93394720:GTG:G	donor_loss	1.0000
12:93395487:A:AG	acceptor_gain	1.0000
12:93395488:G:GG	acceptor_gain	1.0000
12:93395532:AGG:A	donor_loss	1.0000
12:93395534:G:GA	donor_loss	1.0000
12:93398766:A:G	acceptor_gain	1.0000
12:93398767:GCA:G	acceptor_gain	1.0000
12:93398853:TTGGT:T	donor_loss	1.0000
12:93398855:GGT:G	donor_loss	1.0000
12:93398856:G:A	donor_loss	1.0000
12:93398857:T:G	donor_loss	1.0000
12:93399263:C:G	donor_gain	1.0000

AlphaMissense

1167 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
12:93378387:C:A	A22E	1.000
12:93378389:T:C	C23R	1.000
12:93378390:G:A	C23Y	1.000
12:93378391:C:G	C23W	1.000
12:93378395:T:C	C25R	1.000
12:93378396:G:A	C25Y	1.000
12:93378397:C:G	C25W	1.000
12:93394613:T:C	L35P	1.000
12:93394616:T:A	L36Q	1.000
12:93394616:T:C	L36P	1.000
12:93394619:T:A	V37E	1.000
12:93394645:T:A	W46R	1.000
12:93394645:T:C	W46R	1.000
12:93394647:G:C	W46C	1.000
12:93394647:G:T	W46C	1.000
12:93394652:T:A	V48D	1.000
12:93394654:C:T	P49S	1.000
12:93394655:C:A	P49Q	1.000
12:93394655:C:G	P49R	1.000
12:93394658:G:A	G50E	1.000
12:93394660:G:A	G51R	1.000
12:93394660:G:C	G51R	1.000
12:93394661:G:A	G51E	1.000
12:93394664:G:A	G52E	1.000
12:93394697:C:A	A63D	1.000
12:93394703:G:C	R65T	1.000
12:93394703:G:T	R65M	1.000
12:93394704:G:C	R65S	1.000
12:93394704:G:T	R65S	1.000
12:93394706:A:G	E66G	1.000

dbSNP variants (sampled 300 via entrez): RS1000036192 (12:93393531 A>C), RS1000094935 (12:93402348 T>A,C), RS1000319837 (12:93407558 G>T), RS1000325312 (12:93393942 A>G), RS1000368933 (12:93405759 T>A,C), RS1000591747 (12:93396377 C>T), RS1000897841 (12:93377885 A>C,G), RS1000900433 (12:93388852 AT>A), RS1001201046 (12:93382429 G>A), RS1001272032 (12:93408614 G>A), RS1001330313 (12:93377672 C>T), RS1001521714 (12:93377702 A>C), RS1001574137 (12:93377394 C>A,G), RS1001810451 (12:93396833 C>G), RS1001975900 (12:93390095 A>G)

Disease associations

OMIM: gene MIM:609229 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

Study	Trait	p-value
GCST90002387_122	Immature fraction of reticulocytes	3.000000e-13
GCST90002404_141	Red cell distribution width	3.000000e-12

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0009188	Red cell distribution width

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL4295967 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

52 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Benzo(a)pyrene	affects methylation, decreases expression	4
Valproic Acid	affects expression, decreases expression, decreases methylation, increases expression	4
trichostatin A	affects cotreatment, increases expression	3
Vorinostat	affects cotreatment, increases expression	2
Panobinostat	affects cotreatment, increases expression	2
Acetaminophen	increases expression, decreases expression	2
Doxorubicin	decreases expression, affects response to substance	2
Tretinoin	decreases expression, increases expression	2
3-((6-(2-methoxyphenyl)pyrimidin-4-yl)amino)phenyl)methane sulfonamide	decreases expression	1
bisphenol F	affects cotreatment, increases expression	1
methylmercuric chloride	increases expression	1
triphenyl phosphate	affects expression	1
pirinixic acid	affects binding, decreases expression, increases activity	1
bisphenol A	affects cotreatment, increases expression	1
2-methyl-4-isothiazolin-3-one	increases expression	1
beta-lapachone	decreases expression	1
potassium chromate(VI)	affects cotreatment, increases expression	1
4-aminophenylarsenoxide	affects binding, decreases reaction	1
epigallocatechin gallate	affects cotreatment, increases expression	1
K 7174	decreases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, increases expression	1
bisphenol B	increases expression	1
dorsomorphin	affects cotreatment, increases expression	1
bisphenol S	affects cotreatment, increases expression	1
Arsenic Trioxide	affects binding, decreases reaction	1
Leflunomide	increases expression	1
Air Pollutants	decreases expression, increases abundance	1
Carbamazepine	affects expression	1
Dexamethasone	affects cotreatment, increases expression	1
Diethylstilbestrol	increases expression	1

ChEMBL screening assays

4 unique, capped per target: 2 admet, 2 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4259507	ADMET	Substrate activity at N-terminal His-tagged DIPP2 (unknown origin) expressed in Escherichia coli assessed as enzyme-mediated test compound metabolism after 20 mins in presence of Mg2+ by malachite green dye based assay	A synthetic diphosphoinositol phosphate analogue of inositol trisphosphate. — Medchemcomm
CHEMBL4259516	Binding	Stabilization of N-terminal His-tagged DIPP2 (unknown origin) expressed in Escherichia coli assessed as increase in melting temperature at 0.1 mM by SYPRO orange dye based DSF method	A synthetic diphosphoinositol phosphate analogue of inositol trisphosphate. — Medchemcomm

Cellosaurus cell lines

3 cell lines: 3 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_E2F2	HAP1 NUDT4 (-) 1	Cancer cell line	Male
CVCL_E2F3	HAP1 NUDT4 (-) 2	Cancer cell line	Male
CVCL_E2F4	HAP1 NUDT4 (-) 3	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.