OR2T33

Summary

OR2T33 (olfactory receptor family 2 subfamily T member 33, HGNC:31255) is a protein-coding gene on chromosome 1q44, encoding Olfactory receptor 2T33 (Q8NG76). Odorant receptor. It is a selective cancer dependency (DepMap: 60.0% of cell lines).

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome.

Source: NCBI Gene 391195 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 71 total
• Cancer dependency (DepMap): dependent in 60.0% of screened cell lines
• MANE Select transcript: NM_001004695

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31255
Approved symbol	OR2T33
Name	olfactory receptor family 2 subfamily T member 33
Location	1q44
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000177212
Ensembl biotype	protein_coding
Entrez	391195

Gene structure

Transcript identifiers

Ensembl transcripts: 2 — 2 protein_coding

ENST00000318021, ENST00000641220

RefSeq mRNA: 1 — MANE Select: NM_001004695 NM_001004695

CCDS: CCDS31109

Canonical transcript exons

ENST00000641220 — 2 exons

Exon	Start	End
ENSE00003814154	248277765	248277976
ENSE00003814191	248269917	248273822

Expression profiles

Bgee: expression breadth broad, 22 present calls, max score 76.66.

Top tissues by expression

105 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	76.66	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	65.26	gold quality
monocyte	CL:0000576	55.06	gold quality
leukocyte	CL:0000738	54.29	gold quality
stromal cell of endometrium	CL:0002255	47.89	gold quality
colonic epithelium	UBERON:0000397	45.97	gold quality
bone marrow	UBERON:0002371	43.94	gold quality
blood	UBERON:0000178	43.66	gold quality
endometrium	UBERON:0001295	43.54	gold quality
vermiform appendix	UBERON:0001154	43.29	gold quality
placenta	UBERON:0001987	42.81	gold quality
left lobe of thyroid gland	UBERON:0001120	41.78	gold quality
thyroid gland	UBERON:0002046	41.54	gold quality
skeletal muscle tissue	UBERON:0001134	40.86	gold quality
ganglionic eminence	UBERON:0004023	40.56	gold quality
smooth muscle tissue	UBERON:0001135	40.48	silver quality
cortical plate	UBERON:0005343	40.00	gold quality
ventricular zone	UBERON:0003053	39.90	gold quality
prefrontal cortex	UBERON:0000451	39.78	gold quality
right lobe of thyroid gland	UBERON:0001119	39.68	silver quality
muscle of leg	UBERON:0001383	38.66	gold quality
islet of Langerhans	UBERON:0000006	38.58	gold quality
gall bladder	UBERON:0002110	38.38	gold quality
lymph node	UBERON:0000029	38.30	gold quality
liver	UBERON:0002107	38.23	gold quality
primary visual cortex	UBERON:0002436	38.16	silver quality
lower esophagus mucosa	UBERON:0035834	38.02	gold quality
gastrocnemius	UBERON:0001388	37.87	gold quality
frontal cortex	UBERON:0001870	36.67	gold quality
granulocyte	CL:0000094	36.33	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	3.77
E-MTAB-6386	no	79.77

Regulation

Is transcription factor: no

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 60.0% of screened cell lines.

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
mus_musculus	Or2t44	ENSMUSG00000059504
rattus_norvegicus	Or2t44b	ENSRNOG00000033492
rattus_norvegicus	Or2t44	ENSRNOG00000084778
rattus_norvegicus	Olr1454	ENSRNOG00000089012

Paralogs (92): OR2B6 (ENSG00000124657), OR13C9 (ENSG00000136839), OR13C4 (ENSG00000148136), OR2M5 (ENSG00000162727), OR10A5 (ENSG00000166363), OR2D2 (ENSG00000166368), OR2B2 (ENSG00000168131), OR2C1 (ENSG00000168158), OR13J1 (ENSG00000168828), OR10G3 (ENSG00000169208), OR10A3 (ENSG00000170683), OR10A4 (ENSG00000170782), OR10A2 (ENSG00000170790), OR13H1 (ENSG00000171054), OR2K2 (ENSG00000171133), OR2M4 (ENSG00000171180), OR10H2 (ENSG00000171942), OR10H5 (ENSG00000172519), OR10AD1 (ENSG00000172640), OR2Y1 (ENSG00000174339), OR10AG1 (ENSG00000174970), OR2T1 (ENSG00000175143), OR10P1 (ENSG00000175398), OR2T35 (ENSG00000177151), OR2M7 (ENSG00000177186), OR2T12 (ENSG00000177201), OR2AJ1 (ENSG00000177275), OR2T8 (ENSG00000177462), OR2G3 (ENSG00000177476), OR2G2 (ENSG00000177489), OR2B11 (ENSG00000177535), OR2D3 (ENSG00000178358), OR13D1 (ENSG00000179055), OR10A7 (ENSG00000179919), OR2Z1 (ENSG00000181733), OR2V2 (ENSG00000182613), OR10G7 (ENSG00000182634), OR2T29 (ENSG00000182783), OR2T34 (ENSG00000183310), OR2T10 (ENSG00000184022)

Protein

Protein identifiers

Olfactory receptor 2T33 — Q8NG76 (reviewed: Q8NG76)

Alternative names: Olfactory receptor OR1-56

All UniProt accessions (1): Q8NG76

UniProt curated annotations — full annotation on UniProt →

Function. Odorant receptor.

Subcellular location. Cell membrane.

Similarity. Belongs to the G-protein coupled receptor 1 family.

RefSeq proteins (1): NP_001004695* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000276	GPCR_Rhodpsn	Family
IPR000725	Olfact_rcpt	Family
IPR017452	GPCR_Rhodpsn_7TM	Domain

Pfam: PF13853

UniProt features (19 total): topological domain 8, transmembrane region 7, chain 1, glycosylation site 1, disulfide bond 1, sequence variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8NG76-F1	88.47	0.61

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Disulfide bonds (1): 95–187

Glycosylation sites (1): 17

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-9752946	Expression and translocation of olfactory receptors

MSigDB gene sets: 25 (showing top): GOBP_SENSORY_PERCEPTION_OF_CHEMICAL_STIMULUS, KEGG_OLFACTORY_TRANSDUCTION, GOBP_DETECTION_OF_STIMULUS, GOBP_SENSORY_PERCEPTION, chr1q44, GOMF_OLFACTORY_RECEPTOR_ACTIVITY, GOMF_TRANSMEMBRANE_SIGNALING_RECEPTOR_ACTIVITY, GOMF_G_PROTEIN_COUPLED_RECEPTOR_ACTIVITY, GOBP_SENSORY_PERCEPTION_OF_SMELL, GOBP_G_PROTEIN_COUPLED_RECEPTOR_SIGNALING_PATHWAY, GOBP_DETECTION_OF_STIMULUS_INVOLVED_IN_SENSORY_PERCEPTION, REACTOME_OLFACTORY_SIGNALING_PATHWAY, LAMB3_TARGET_GENES, REACTOME_SENSORY_PERCEPTION, GSE24102_GRANULOCYSTIC_MDSC_VS_NEUTROPHIL_DN

GO Biological Process (4): detection of chemical stimulus involved in sensory perception of smell (GO:0050911), signal transduction (GO:0007165), G protein-coupled receptor signaling pathway (GO:0007186), sensory perception of smell (GO:0007608)

GO Molecular Function (2): G protein-coupled receptor activity (GO:0004930), olfactory receptor activity (GO:0004984)

GO Cellular Component (2): plasma membrane (GO:0005886), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
Olfactory Signaling Pathway	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
transmembrane signaling receptor activity	2
sensory perception of smell	1
detection of chemical stimulus involved in sensory perception	1
cell communication	1
cellular process	1
signaling	1
regulation of cellular process	1
cellular response to stimulus	1
G protein-coupled receptor activity	1
signal transduction	1
sensory perception of chemical stimulus	1
G protein-coupled receptor signaling pathway	1
detection of chemical stimulus involved in sensory perception of smell	1
membrane	1
cell periphery	1
cellular anatomical structure	1

Protein interactions and networks

STRING

158 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
OR2T33	TAS2R13	Q9NYV9	528
OR2T33	TAS2R5	Q9NYW4	505
OR2T33	TAS2R50	P59544	505
OR2T33	VN1R4	Q7Z5H5	479
OR2T33	GOLGA6L6	A8MZA4	474
OR2T33	GOLGA6L1	Q8N7Z2	450
OR2T33	TAS2R10	Q9NYW0	447
OR2T33	VN1R2	Q8NFZ6	447
OR2T33	TAS2R14	Q9NYV8	447
OR2T33	TAS2R43	P59537	446
OR2T33	PPIAL4G	P0DN37	436
OR2T33	VN1R1	Q9GZP7	391
OR2T33	TRIML1	Q8N9V2	370
OR2T33	LYPD5	Q6UWN5	370
OR2T33	TAS2R4	Q9NYW5	360

IntAct

2 interactions, top by confidence:

A	B	Type	Score
OR2T33	ANKLE2	psi-mi:“MI:0914”(association)	0.350

BioGRID (2): ANKLE2 (Affinity Capture-MS), OR2T12 (Affinity Capture-MS)

ESM2 similar proteins: A6NH00, O60403, O95371, O95918, P23266, P23267, P23275, P34984, P47881, P47888, P47893, Q15619, Q5JQS5, Q5TZ20, Q60885, Q60891, Q60894, Q6IEZ7, Q7Z3T1, Q8N628, Q8NG76, Q8NG77, Q8NG97, Q8NGA6, Q8NGC4, Q8NGE3, Q8NGE9, Q8NGQ2, Q8NGQ4, Q8NGR4, Q8NGS0, Q8NGT9, Q8NGX9, Q8NGY1, Q8NGZ6, Q8NH02, Q8NH03, Q8NH04, Q8NHB1, Q8VGD6

Diamond homologs: A3KFT3, A4D2G3, A6NH00, A6NL26, A6NM03, O43869, O76000, O76001, O76002, O95006, O95221, O95371, O95918, P0C7N1, P0C7T2, P23274, P23275, P34984, P59922, Q13606, Q13607, Q5TZ20, Q60880, Q60890, Q6IEZ7, Q6IF00, Q8N127, Q8N349, Q8N628, Q8NG75, Q8NG76, Q8NG77, Q8NG80, Q8NG81, Q8NG83, Q8NG84, Q8NG85, Q8NG97, Q8NGC0, Q8NGE5

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

71 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	66
Likely benign	4
Benign	1

Top pathogenic / likely-pathogenic (0)

SpliceAI

395 predictions. Top by Δscore:

Variant	Effect	Δscore
1:248273166:CA:C	acceptor_gain	0.7700
1:248272949:C:CC	acceptor_gain	0.7200
1:248273167:A:C	acceptor_gain	0.7200
1:248273554:A:AC	donor_gain	0.7100
1:248273555:C:CC	donor_gain	0.7100
1:248273223:A:AC	donor_gain	0.6900
1:248273224:C:CC	donor_gain	0.6900
1:248273167:A:AC	acceptor_gain	0.6800
1:248273495:C:CT	donor_gain	0.6800
1:248272945:CACA:C	acceptor_gain	0.6700
1:248273496:C:CT	donor_gain	0.6700
1:248273557:T:TA	donor_gain	0.6700
1:248272947:CA:C	acceptor_gain	0.6600
1:248273167:A:T	acceptor_gain	0.6500
1:248273268:CCAG:C	donor_gain	0.6500
1:248273154:C:CT	acceptor_gain	0.6300
1:248273267:A:AC	donor_gain	0.6300
1:248273268:C:CC	donor_gain	0.6300
1:248273555:CTT:C	donor_gain	0.6200
1:248273165:CCA:C	acceptor_gain	0.6100
1:248273262:AACG:A	donor_loss	0.6100
1:248273263:ACGC:A	donor_loss	0.6100
1:248273264:CGCA:C	donor_loss	0.6100
1:248273265:GCA:G	donor_loss	0.6100
1:248273266:CA:C	donor_loss	0.6100
1:248273725:CAGA:C	donor_gain	0.6100
1:248273726:AGAA:A	donor_gain	0.6100
1:248273127:C:CC	acceptor_gain	0.6000
1:248273261:AAAC:A	donor_loss	0.6000
1:248272946:ACA:A	acceptor_gain	0.5800

AlphaMissense

2098 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:248272948:A:C	S289R	0.880
1:248272948:A:T	S289R	0.880
1:248272950:T:G	S289R	0.880
1:248273785:A:C	F10L	0.878
1:248273785:A:T	F10L	0.878
1:248273787:A:G	F10L	0.878
1:248273290:G:C	F175L	0.864
1:248273290:G:T	F175L	0.864
1:248273292:A:G	F175L	0.864
1:248273236:G:C	F193L	0.841
1:248273236:G:T	F193L	0.841
1:248273238:A:G	F193L	0.841
1:248273068:A:C	F249L	0.832
1:248273068:A:T	F249L	0.832
1:248273070:A:G	F249L	0.832
1:248273287:G:C	F176L	0.826
1:248273287:G:T	F176L	0.826
1:248273289:A:G	F176L	0.826
1:248273467:C:A	M116I	0.795
1:248273467:C:G	M116I	0.795
1:248273467:C:T	M116I	0.795
1:248273722:A:C	S31R	0.794
1:248273722:A:T	S31R	0.794
1:248273724:T:G	S31R	0.794
1:248273767:A:C	F16L	0.773
1:248273767:A:T	F16L	0.773
1:248273769:A:G	F16L	0.773
1:248272990:G:C	F275L	0.769
1:248272990:G:T	F275L	0.769
1:248272992:A:G	F275L	0.769

dbSNP variants (sampled 300 via entrez): RS1000342772 (1:248269634 T>C), RS1000774481 (1:248269882 T>C), RS1000802094 (1:248279432 C>G), RS1002350253 (1:248273105 G>A), RS1002381457 (1:248273714 A>C,G), RS1002718260 (1:248275135 T>C), RS1004124574 (1:248269530 T>C), RS1004319955 (1:248275575 C>T), RS1005697453 (1:248270406 C>T), RS1005797378 (1:248271019 A>T), RS1005952955 (1:248278987 T>C), RS1006027997 (1:248271751 T>C), RS1006028905 (1:248277435 T>C), RS1006336283 (1:248278713 A>G), RS1006714197 (1:248277544 C>G,T)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

4 total (human), top 4 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Resveratrol	affects cotreatment, decreases expression	2
2-palmitoylglycerol	increases expression	1
Copper	affects cotreatment, decreases expression	1
Plant Extracts	affects cotreatment, decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.