OR2T35

Summary

OR2T35 (olfactory receptor family 2 subfamily T member 35 (gene/pseudogene), HGNC:31257) is a protein-coding gene on chromosome 1q44, encoding Olfactory receptor 2T35 (Q8NGX2). Odorant receptor.

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome.

Source: NCBI Gene 403244 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 54 total
• MANE Select transcript: NM_001001827

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31257
Approved symbol	OR2T35
Name	olfactory receptor family 2 subfamily T member 35 (gene/pseudogene)
Location	1q44
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000177151
Ensembl biotype	protein_coding
Entrez	403244

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000641268

RefSeq mRNA: 1 — MANE Select: NM_001001827 NM_001001827

CCDS: CCDS31123

Canonical transcript exons

ENST00000641268 — 2 exons

Exon	Start	End
ENSE00003812515	248645247	248645278
ENSE00003813522	248636356	248639280

Expression profiles

Bgee: expression breadth tissue_specific, 2 present calls, max score 38.05.

Top tissues by expression

128 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
bone marrow cell	CL:0002092	38.05	gold quality
colonic epithelium	UBERON:0000397	37.20	gold quality
ventricular zone	UBERON:0003053	36.48	gold quality
cortical plate	UBERON:0005343	36.47	gold quality
ganglionic eminence	UBERON:0004023	35.49	gold quality
bone marrow	UBERON:0002371	34.87	gold quality
skeletal muscle tissue	UBERON:0001134	33.38	gold quality
hindlimb stylopod muscle	UBERON:0004252	32.15	gold quality
prefrontal cortex	UBERON:0000451	31.23	gold quality
muscle tissue	UBERON:0002385	31.06	gold quality
placenta	UBERON:0001987	30.88	gold quality
tonsil	UBERON:0002372	30.83	gold quality
stromal cell of endometrium	CL:0002255	29.87	gold quality
kidney	UBERON:0002113	29.00	silver quality
lymph node	UBERON:0000029	28.71	gold quality
right uterine tube	UBERON:0001302	28.59	gold quality
liver	UBERON:0002107	28.52	gold quality
duodenum	UBERON:0002114	28.14	gold quality
monocyte	CL:0000576	27.79	gold quality
leukocyte	CL:0000738	27.77	gold quality
primary visual cortex	UBERON:0002436	27.36	gold quality
islet of Langerhans	UBERON:0000006	26.88	gold quality
superior frontal gyrus	UBERON:0002661	26.79	gold quality
adult mammalian kidney	UBERON:0000082	26.52	silver quality
vermiform appendix	UBERON:0001154	26.42	gold quality
gall bladder	UBERON:0002110	25.98	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	25.89	gold quality
cortex of kidney	UBERON:0001225	25.85	gold quality
urinary bladder	UBERON:0001255	25.72	gold quality
frontal cortex	UBERON:0001870	25.63	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.46

Regulation

Is transcription factor: no

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Or2t35	ENSMUSG00000091809
rattus_norvegicus	Or2t35	ENSRNOG00000084001

Paralogs (92): OR2B6 (ENSG00000124657), OR13C9 (ENSG00000136839), OR13C4 (ENSG00000148136), OR2M5 (ENSG00000162727), OR10A5 (ENSG00000166363), OR2D2 (ENSG00000166368), OR2B2 (ENSG00000168131), OR2C1 (ENSG00000168158), OR13J1 (ENSG00000168828), OR10G3 (ENSG00000169208), OR10A3 (ENSG00000170683), OR10A4 (ENSG00000170782), OR10A2 (ENSG00000170790), OR13H1 (ENSG00000171054), OR2K2 (ENSG00000171133), OR2M4 (ENSG00000171180), OR10H2 (ENSG00000171942), OR10H5 (ENSG00000172519), OR10AD1 (ENSG00000172640), OR2Y1 (ENSG00000174339), OR10AG1 (ENSG00000174970), OR2T1 (ENSG00000175143), OR10P1 (ENSG00000175398), OR2M7 (ENSG00000177186), OR2T12 (ENSG00000177201), OR2T33 (ENSG00000177212), OR2AJ1 (ENSG00000177275), OR2T8 (ENSG00000177462), OR2G3 (ENSG00000177476), OR2G2 (ENSG00000177489), OR2B11 (ENSG00000177535), OR2D3 (ENSG00000178358), OR13D1 (ENSG00000179055), OR10A7 (ENSG00000179919), OR2Z1 (ENSG00000181733), OR2V2 (ENSG00000182613), OR10G7 (ENSG00000182634), OR2T29 (ENSG00000182783), OR2T34 (ENSG00000183310), OR2T10 (ENSG00000184022)

Protein

Protein identifiers

Olfactory receptor 2T35 — Q8NGX2 (reviewed: Q8NGX2)

Alternative names: Olfactory receptor OR1-66

All UniProt accessions (1): Q8NGX2

UniProt curated annotations — full annotation on UniProt →

Function. Odorant receptor.

Subcellular location. Cell membrane.

Similarity. Belongs to the G-protein coupled receptor 1 family.

RefSeq proteins (1): NP_001001827* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000276	GPCR_Rhodpsn	Family
IPR000725	Olfact_rcpt	Family
IPR017452	GPCR_Rhodpsn_7TM	Domain

Pfam: PF13853

UniProt features (18 total): topological domain 8, transmembrane region 7, chain 1, glycosylation site 1, disulfide bond 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8NGX2-F1	89.01	0.68

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Disulfide bonds (1): 98–189

Glycosylation sites (1): 9

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-9752946	Expression and translocation of olfactory receptors

MSigDB gene sets: 19 (showing top): GOBP_SENSORY_PERCEPTION_OF_CHEMICAL_STIMULUS, ACEVEDO_LIVER_CANCER_WITH_H3K27ME3_UP, GOBP_DETECTION_OF_STIMULUS, GOBP_SENSORY_PERCEPTION, chr1q44, ACEVEDO_METHYLATED_IN_LIVER_CANCER_DN, GOMF_OLFACTORY_RECEPTOR_ACTIVITY, GOMF_TRANSMEMBRANE_SIGNALING_RECEPTOR_ACTIVITY, GOMF_G_PROTEIN_COUPLED_RECEPTOR_ACTIVITY, GOBP_SENSORY_PERCEPTION_OF_SMELL, GOBP_G_PROTEIN_COUPLED_RECEPTOR_SIGNALING_PATHWAY, GOBP_DETECTION_OF_STIMULUS_INVOLVED_IN_SENSORY_PERCEPTION, REACTOME_OLFACTORY_SIGNALING_PATHWAY, GAUCHER_PBMC_YF_VAX_STAMARIL_UNKNOWN_AGE_60DY_DN, REACTOME_SENSORY_PERCEPTION

GO Biological Process (4): detection of chemical stimulus involved in sensory perception of smell (GO:0050911), signal transduction (GO:0007165), G protein-coupled receptor signaling pathway (GO:0007186), sensory perception of smell (GO:0007608)

GO Molecular Function (2): G protein-coupled receptor activity (GO:0004930), olfactory receptor activity (GO:0004984)

GO Cellular Component (2): plasma membrane (GO:0005886), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
Olfactory Signaling Pathway	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
transmembrane signaling receptor activity	2
sensory perception of smell	1
detection of chemical stimulus involved in sensory perception	1
cell communication	1
cellular process	1
signaling	1
regulation of cellular process	1
cellular response to stimulus	1
G protein-coupled receptor activity	1
signal transduction	1
sensory perception of chemical stimulus	1
G protein-coupled receptor signaling pathway	1
detection of chemical stimulus involved in sensory perception of smell	1
membrane	1
cell periphery	1
cellular anatomical structure	1

Protein interactions and networks

STRING

170 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
OR2T35	CCDC74A	Q96AQ1	507
OR2T35	KRTAP4-5	Q9BYR2	417
OR2T35	VCF2	Q5XKR9	417
OR2T35	OVCH2	Q7RTZ1	397
OR2T35	FRG2C	A6NGY1	393
OR2T35	ANKRD36B	Q8N2N9	391
OR2T35	IRGQ	Q8WZA9	376
OR2T35	ZNF417	Q8TAU3	359
OR2T35	CYLC1	P35663	348
OR2T35	TMEM212	A6NML5	336
OR2T35	AHNAK2	Q8IVF2	314
OR2T35	AKR1B15	C9JRZ8	312
OR2T35	SMTNL2	Q2TAL5	311
OR2T35	GXYLT1	Q4G148	310
OR2T35	CORO6	Q6QEF8	307

IntAct

2 interactions, top by confidence:

A	B	Type	Score
CUL3	PXDNL	psi-mi:“MI:0914”(association)	0.350

BioGRID (3): OR2T35 (Affinity Capture-MS), OR2T35 (Affinity Capture-MS), OR2T35 (Affinity Capture-Luminescence)

ESM2 similar proteins: A6NL26, O43749, O76001, O76002, O76100, O95006, P0C7T2, P0DMU2, P23272, P23274, P30955, P58173, P59922, P70526, Q13607, Q60890, Q6IEU7, Q6IEZ7, Q6IF00, Q6IF36, Q6UXT6, Q8NGE5, Q8NGI7, Q8NGL2, Q8NGN7, Q8NGP8, Q8NGQ2, Q8NGS0, Q8NGS2, Q8NGX2, Q8NGX3, Q8NGZ4, Q8NGZ5, Q8NH01, Q8NH02, Q8NH04, Q8NH80, Q8NHA6, Q8VEY3, Q8VG44

Diamond homologs: A3KFT3, A4D2G3, A6NH00, A6NL26, A6NM03, O43869, O76000, O76001, O76002, O95006, O95221, O95371, O95918, P0C7N1, P0C7T2, P23274, P23275, P34984, P59922, Q13606, Q13607, Q5TZ20, Q60880, Q60890, Q6IEZ7, Q6IF00, Q8N127, Q8N349, Q8N628, Q8NG75, Q8NG76, Q8NG77, Q8NG80, Q8NG81, Q8NG83, Q8NG84, Q8NG85, Q8NG97, Q8NGC0, Q8NGE5

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

54 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	52
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

259 predictions. Top by Δscore:

Variant	Effect	Δscore
1:248638823:C:CT	donor_gain	0.9800
1:248638893:G:C	donor_gain	0.9800
1:248638824:C:CT	donor_gain	0.9500
1:248638838:AAAC:A	donor_gain	0.9200
1:248638840:AC:A	donor_gain	0.9100
1:248638841:CC:C	donor_gain	0.9100
1:248638990:T:TA	donor_gain	0.9100
1:248638842:CC:C	donor_gain	0.8000
1:248638962:TG:T	donor_gain	0.7900
1:248638839:A:T	donor_gain	0.7800
1:248638603:G:C	acceptor_gain	0.7600
1:248638932:T:TA	donor_gain	0.7600
1:248638834:AAG:A	donor_gain	0.7500
1:248638663:A:C	donor_gain	0.7300
1:248638901:AGG:A	donor_gain	0.7200
1:248638884:AG:A	donor_gain	0.7100
1:248638843:C:T	donor_gain	0.6900
1:248638839:AAC:A	donor_gain	0.6400
1:248638602:CG:C	acceptor_gain	0.6200
1:248638750:C:CC	acceptor_gain	0.6200
1:248639005:T:TA	donor_gain	0.6200
1:248638822:A:AC	donor_gain	0.6000
1:248638664:T:TA	donor_gain	0.5900
1:248638892:AG:A	donor_gain	0.5800
1:248638599:G:C	acceptor_gain	0.5700
1:248638804:C:CT	donor_gain	0.5600
1:248638885:G:C	donor_gain	0.5600
1:248638717:ATCTC:A	acceptor_gain	0.5500
1:248638661:A:AC	donor_gain	0.5400
1:248638662:C:CC	donor_gain	0.5400

AlphaMissense

2122 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:248638512:G:C	S249R	0.942
1:248638512:G:T	S249R	0.942
1:248638514:T:G	S249R	0.942
1:248638386:G:C	S291R	0.941
1:248638386:G:T	S291R	0.941
1:248638388:T:G	S291R	0.941
1:248639220:G:C	F13L	0.926
1:248639220:G:T	F13L	0.926
1:248639222:A:G	F13L	0.926
1:248638728:A:C	F177L	0.922
1:248638728:A:T	F177L	0.922
1:248638730:A:G	F177L	0.922
1:248638905:C:A	M118I	0.916
1:248638905:C:G	M118I	0.916
1:248638905:C:T	M118I	0.916
1:248639033:A:G	C76R	0.902
1:248638506:G:C	F251L	0.901
1:248638506:G:T	F251L	0.901
1:248638508:A:G	F251L	0.901
1:248638755:G:C	F168L	0.894
1:248638755:G:T	F168L	0.894
1:248638757:A:G	F168L	0.894
1:248638894:C:G	R122P	0.889
1:248638725:G:C	F178L	0.887
1:248638725:G:T	F178L	0.887
1:248638727:A:G	F178L	0.887
1:248638401:G:C	N286K	0.886
1:248638401:G:T	N286K	0.886
1:248638723:C:G	C179S	0.882
1:248638724:A:T	C179S	0.882

dbSNP variants (sampled 300 via entrez): RS1003670296 (1:248639364 T>TGA), RS1004107826 (1:248638518 C>A,T), RS1005064822 (1:248643110 A>C,G), RS1005097323 (1:248642345 C>A,T), RS1009504752 (1:248639246 C>A,G), RS1009534401 (1:248638456 G>A), RS1010512266 (1:248645293 A>G), RS1010541630 (1:248644625 G>A), RS1010689082 (1:248645524 T>A,C,G), RS1012073500 (1:248647164 G>A), RS1012097514 (1:248645882 AAG>A), RS1019620655 (1:248642382 C>T), RS1020970216 (1:248638473 G>C), RS1021001289 (1:248636504 C>T), RS1021979374 (1:248644653 G>A)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

4 total (human), top 4 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Resveratrol	affects cotreatment, decreases expression	1
Atrazine	increases expression	1
Plant Extracts	affects cotreatment, decreases expression	1
Aflatoxin B1	increases methylation	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.