OTUD3

Summary

OTUD3 (OTU deubiquitinase 3, HGNC:29038) is a protein-coding gene on chromosome 1p36.13, encoding OTU domain-containing protein 3 (Q5T2D3). Deubiquitinating enzyme that hydrolyzes ‘Lys-6’- and ‘Lys-11’-linked polyubiquitin.

Enables cysteine-type deubiquitinase activity. Involved in cellular response to nutrient levels and protein deubiquitination. Acts upstream of or within negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; protein deubiquitination; and protein stabilization. Located in cytoplasm. Is active in nucleus.

Source: NCBI Gene 23252 — RefSeq curated summary.

At a glance

• GWAS associations: 11
• Clinical variants (ClinVar): 47 total
• Druggable target: yes
• MANE Select transcript: NM_015207

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:29038
Approved symbol	OTUD3
Name	OTU deubiquitinase 3
Location	1p36.13
Locus type	gene with protein product
Status	Approved
Aliases	KIAA0459, DUBA4
Ensembl gene	ENSG00000169914
Ensembl biotype	protein_coding
OMIM	611758
Entrez	23252

Gene structure

Transcript identifiers

Ensembl transcripts: 7 — 5 protein_coding, 2 protein_coding_CDS_not_defined

ENST00000375120, ENST00000466697, ENST00000481686, ENST00000880253, ENST00000880254, ENST00000916790, ENST00000916791

RefSeq mRNA: 1 — MANE Select: NM_015207 NM_015207

CCDS: CCDS41279

Canonical transcript exons

ENST00000375120 — 8 exons

Exon	Start	End
ENSE00001160073	19906432	19906616
ENSE00001181367	19907570	19912945
ENSE00001263611	19904891	19904987
ENSE00001465814	19904267	19904398
ENSE00001465815	19897540	19897662
ENSE00001465817	19890385	19890533
ENSE00001465818	19882395	19882734
ENSE00003572026	19894368	19894480

Expression profiles

Bgee: expression breadth ubiquitous, 218 present calls, max score 96.58.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 13.4127 / max 159.4089, expressed in 1770 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
1085	13.4127	1770

Top tissues by expression

273 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
oocyte	CL:0000023	96.58	gold quality
secondary oocyte	CL:0000655	95.66	gold quality
right uterine tube	UBERON:0001302	85.16	gold quality
adrenal tissue	UBERON:0018303	84.71	gold quality
body of uterus	UBERON:0009853	84.04	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	83.26	gold quality
cerebellar hemisphere	UBERON:0002245	83.17	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	83.07	gold quality
cerebellar cortex	UBERON:0002129	83.04	gold quality
right hemisphere of cerebellum	UBERON:0014890	82.95	gold quality
cerebellum	UBERON:0002037	81.66	gold quality
muscle layer of sigmoid colon	UBERON:0035805	80.94	gold quality
left ovary	UBERON:0002119	80.79	gold quality
calcaneal tendon	UBERON:0003701	80.53	gold quality
left testis	UBERON:0004533	80.33	gold quality
right testis	UBERON:0004534	80.33	gold quality
ectocervix	UBERON:0012249	80.31	gold quality
testis	UBERON:0000473	80.24	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	80.19	gold quality
right ovary	UBERON:0002118	80.10	gold quality
endocervix	UBERON:0000458	79.88	gold quality
lower esophagus	UBERON:0013473	79.83	gold quality
lower esophagus muscularis layer	UBERON:0035833	79.83	gold quality
right adrenal gland cortex	UBERON:0035827	79.68	gold quality
Brodmann (1909) area 9	UBERON:0013540	79.60	gold quality
left adrenal gland cortex	UBERON:0035825	79.18	gold quality
ovary	UBERON:0000992	79.03	gold quality
left adrenal gland	UBERON:0001234	78.88	gold quality
sural nerve	UBERON:0015488	78.76	gold quality
rectum	UBERON:0001052	78.71	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-GEOD-100618	yes	230.04
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

142 targeting OTUD3, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-9-5P	100.00	72.28	2361
HSA-MIR-4481	100.00	66.42	1669
HSA-MIR-450A-1-3P	100.00	69.33	1837
HSA-MIR-4673	100.00	66.64	1490
HSA-MIR-4682	100.00	68.89	1258
HSA-MIR-10401-5P	99.99	65.79	948
HSA-MIR-371B-5P	99.99	75.34	4759
HSA-MIR-3662	99.99	73.82	5684
HSA-MIR-150-5P	99.99	66.69	1976
HSA-MIR-363-3P	99.98	74.72	1821
HSA-MIR-32-5P	99.98	75.21	1964
HSA-MIR-92A-3P	99.98	75.21	1960
HSA-MIR-92B-3P	99.98	75.25	1955
HSA-MIR-25-3P	99.98	74.60	1817
HSA-MIR-367-3P	99.98	74.83	1819
HSA-MIR-4775	99.98	75.00	6394
HSA-MIR-373-5P	99.98	75.36	4753
HSA-MIR-616-5P	99.98	75.58	4775
HSA-MIR-4745-5P	99.98	65.95	1028
HSA-MIR-3692-3P	99.98	70.27	2139
HSA-MIR-4645-5P	99.98	65.81	1284
HSA-MIR-1229-3P	99.97	66.49	906
HSA-MIR-6780B-5P	99.96	69.60	2562
HSA-MIR-4725-3P	99.96	69.53	2520
HSA-MIR-570-3P	99.96	72.41	4910
HSA-MIR-548AJ-3P	99.96	73.38	5345
HSA-MIR-548X-3P	99.96	73.38	5345
HSA-MIR-548AA	99.96	70.64	3753
HSA-MIR-548AP-3P	99.96	70.64	3753

Literature-anchored findings (GeneRIF, showing 12)

• Single-nucleotide polymorphism in OTUD3 gene is associated with ulcerative colitis. (PMID:23511034)
• OTUD3 is an essential regulator of PTEN and the OTUD3-PTEN signalling axis plays a critical role in tumour suppression. (PMID:26280536)
• Knockdown of OTUD3 results in a decrease in the level of GRP78 protein, suppression of cell growth and migration, and tumorigenesis in lung cancer. (PMID:31266968)
• MicroRNA 32 promotes cell proliferation, migration, and suppresses apoptosis in colon cancer cells by targeting OTU domain containing 3. (PMID:31338872)
• Ubiquitin ligase CHIP regulates OTUD3 stability and suppresses tumour metastasis in lung cancer. (PMID:32483383)
• Tumor suppressor OTUD3 induces growth inhibition and apoptosis by directly deubiquitinating and stabilizing p53 in invasive breast carcinoma cells. (PMID:32571254)
• Acetylation-Dependent Deubiquitinase OTUD3 Controls MAVS Activation in Innate Antiviral Immunity. (PMID:32679077)
• miR-520h Stimulates Drug Resistance to Paclitaxel by Targeting the OTUD3-PTEN Axis in Breast Cancer. (PMID:32775453)
• The deubiquitinase OTUD3 stabilizes ACTN4 to drive growth and metastasis of hepatocellular carcinoma. (PMID:34380780)
• Nicotine-mediated OTUD3 downregulation inhibits VEGF-C mRNA decay to promote lymphatic metastasis of human esophageal cancer. (PMID:34853315)
• Silencing of LncRNA SNHG6 protects trophoblast cells through regulating miR-101-3p/OTUD3 axis in unexplained recurrent spontaneous abortion. (PMID:36173586)
• Prevalence and clinical characteristics of T2DM patients with OTUD3 gene rs78466831 SNP at a single academic center in China. (PMID:36531510)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	otud3	ENSDARG00000022648
mus_musculus	Otud3	ENSMUSG00000041161
rattus_norvegicus	Otud3	ENSRNOG00000017037
drosophila_melanogaster	otu	FBGN0003023
drosophila_melanogaster	CG3251	FBGN0031622

Paralogs (5): OTUD5 (ENSG00000068308), OTUD6B (ENSG00000155100), OTUD4 (ENSG00000164164), OTUD1 (ENSG00000165312), OTUD6A (ENSG00000189401)

Protein

Protein identifiers

OTU domain-containing protein 3 — Q5T2D3 (reviewed: Q5T2D3)

All UniProt accessions (1): Q5T2D3

UniProt curated annotations — full annotation on UniProt →

Function. Deubiquitinating enzyme that hydrolyzes ‘Lys-6’- and ‘Lys-11’-linked polyubiquitin. Also hydrolyzes heterotypic (mixed and branched) and homotypic chains. Important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation. Also acts as a negative regulator of the ribosome quality control (RQC) by mediating deubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS20/uS10, thereby antagonizing ZNF598-mediated 40S ubiquitination.

Subcellular location. Cytoplasm. Nucleus.

Post-translational modifications. Glucose and fatty acids stimulate CREBBP-dependent acetylation, promoting its nuclear translocation.

Domain organisation. The UBA-like domain has no influence on ubiquitin hydrolysis. Specificity is given by the S1’ ubiquitin-binding site within the OTU domain composed of the Cys-, His- and Variable-loops.

RefSeq proteins (1): NP_056022* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR003323	OTU_dom	Domain
IPR038765	Papain-like_cys_pep_sf	Homologous_superfamily
IPR050704	Peptidase_C85-like	Family

Pfam: PF02338

UniProt features (41 total): modified residue 8, helix 7, strand 6, region of interest 5, compositionally biased region 3, active site 3, sequence variant 3, domain 2, mutagenesis site 2, chain 1, turn 1

Structure

Experimental structures (PDB)

1 structures.

PDB	Method	Resolution (Å)
4BOU	X-RAY DIFFRACTION	1.55

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q5T2D3-F1	71.34	0.29

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (3): 73; 76 (nucleophile); 182

Post-translational modifications (8): 66, 105, 122, 129, 220, 224, 292, 322

Mutagenesis-validated functional residues (2):

Position	Phenotype
76	abolished deubiquitinase activity.
178–181	impaired activity.

Function

Pathways and Gene Ontology

Reactome pathways

2 pathways

ID	Pathway
R-HSA-5689896	Ovarian tumor domain proteases
R-HSA-8948751	Regulation of PTEN stability and activity

MSigDB gene sets: 115 (showing top): GSE45365_NK_CELL_VS_CD8_TCELL_DN, GSE18804_SPLEEN_MACROPHAGE_VS_COLON_TUMORAL_MACROPHAGE_DN, WANG_CLIM2_TARGETS_UP, GOBP_PROTEIN_MODIFICATION_BY_SMALL_PROTEIN_REMOVAL, GOBP_NEGATIVE_REGULATION_OF_INTRACELLULAR_SIGNAL_TRANSDUCTION, SHEDDEN_LUNG_CANCER_GOOD_SURVIVAL_A5, MODULE_205, GOBP_NEGATIVE_REGULATION_OF_PHOSPHATIDYLINOSITOL_3_KINASE_PROTEIN_KINASE_B_SIGNAL_TRANSDUCTION, GOMF_CYSTEINE_TYPE_PEPTIDASE_ACTIVITY, GOBP_PHOSPHATIDYLINOSITOL_3_KINASE_PROTEIN_KINASE_B_SIGNAL_TRANSDUCTION, GOBP_PROTEIN_STABILIZATION, LIAO_METASTASIS, GOBP_POST_TRANSLATIONAL_PROTEIN_MODIFICATION, GOBP_REGULATION_OF_PROTEIN_STABILITY, GOBP_PROTEIN_K48_LINKED_DEUBIQUITINATION

GO Biological Process (13): proteolysis (GO:0006508), protein deubiquitination (GO:0016579), cellular response to nutrient levels (GO:0031669), protein K11-linked deubiquitination (GO:0035871), protein K6-linked deubiquitination (GO:0044313), protein stabilization (GO:0050821), negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction (GO:0051898), protein K48-linked deubiquitination (GO:0071108), protein K27-linked deubiquitination (GO:1990167), regulation of transcription by RNA polymerase II (GO:0006357), ribosome disassembly (GO:0032790), rescue of stalled cytosolic ribosome (GO:0072344), energy homeostasis (GO:0097009)

GO Molecular Function (5): cysteine-type deubiquitinase activity (GO:0004843), protein binding (GO:0005515), peptidase activity (GO:0008233), cysteine-type peptidase activity (GO:0008234), hydrolase activity (GO:0016787)

GO Cellular Component (3): nucleus (GO:0005634), cytoplasm (GO:0005737), cytosol (GO:0005829)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
Deubiquitination	1
PTEN Regulation	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
protein deubiquitination	4
cellular anatomical structure	2
protein metabolic process	1
cysteine-type deubiquitinase activity	1
protein modification by small protein removal	1
response to nutrient levels	1
cellular response to stimulus	1
regulation of protein stability	1
phosphatidylinositol 3-kinase/protein kinase B signal transduction	1
regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	1
negative regulation of intracellular signal transduction	1
regulation of DNA-templated transcription	1
transcription by RNA polymerase II	1
organelle disassembly	1
cytoplasmic translational elongation	1
ribosome disassembly	1
multicellular organismal-level homeostasis	1
cysteine-type peptidase activity	1
deubiquitinase activity	1
binding	1
hydrolase activity	1
catalytic activity, acting on a protein	1
peptidase activity	1
catalytic activity	1
intracellular membrane-bounded organelle	1
intracellular anatomical structure	1
cytoplasm	1

Protein interactions and networks

STRING

2214 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
OTUD3	OTUB1	Q96FW1	746
OTUD3	OTULIN	Q96BN8	717
OTUD3	ZRANB1	Q9UGI0	713
OTUD3	OTUD7B	Q6GQQ9	692
OTUD3	YOD1	Q5VVQ6	671
OTUD3	USP2	O75604	662
OTUD3	OTUD7A	Q8TE49	652
OTUD3	OTUB2	Q96DC9	633
OTUD3	ZUP1	Q96AP4	631
OTUD3	RNF186	Q9NXI6	620
OTUD3	OTUD4	Q01804	615
OTUD3	ZNF598	Q86UK7	615
OTUD3	UCHL3	P15374	595
OTUD3	PTEN	P60484	555
OTUD3	PLA2G2E	Q9NZK7	523

IntAct

25 interactions, top by confidence:

A	B	Type	Score
KPTN	OTUD3	psi-mi:“MI:0915”(physical association)	0.640
PTEN	OTUD3	psi-mi:“MI:0915”(physical association)	0.590
OTUD3	PTEN	psi-mi:“MI:0915”(physical association)	0.590
HSD17B14	OTUD3	psi-mi:“MI:0915”(physical association)	0.560
OTUD3	HSD17B14	psi-mi:“MI:0915”(physical association)	0.560
OTUD3	ITFG2	psi-mi:“MI:0915”(physical association)	0.560
OTUD3	OTUD3	psi-mi:“MI:0915”(physical association)	0.400
OTUD3	KICS2	psi-mi:“MI:0915”(physical association)	0.400
OTUD3	MLF1	psi-mi:“MI:0914”(association)	0.350
OTUD3	GNAT3	psi-mi:“MI:0914”(association)	0.350
OTUD3	MIB1	psi-mi:“MI:0914”(association)	0.350

BioGRID (147): OTUD3 (Affinity Capture-Western), OTUD3 (Reconstituted Complex), PTEN (Affinity Capture-Western), PTEN (Biochemical Activity), OTUD3 (Affinity Capture-Western), OTUD3 (Reconstituted Complex), UBC (Biochemical Activity), OTUD3 (Affinity Capture-RNA), OTUD3 (Reconstituted Complex), OTUD3 (Two-hybrid), MYH9 (Affinity Capture-MS), YBX1 (Affinity Capture-MS), DHX9 (Affinity Capture-MS), HSPA5 (Affinity Capture-MS), IRS4 (Affinity Capture-MS)

ESM2 similar proteins: A6QR44, B1AZ99, B2RUR8, E1C213, E7F6T8, F4K3M6, O70523, O94988, P35922, P42331, P51114, P51115, P51116, P55265, Q06787, Q12789, Q155Q3, Q1LZ89, Q2TBT7, Q3B8D4, Q3ULM0, Q53NI2, Q5BJ56, Q5T2D3, Q5ZLY0, Q61584, Q63505, Q69Z66, Q6GQQ9, Q6PFK1, Q75LL6, Q7TN31, Q80WE1, Q86XL3, Q8BYW1, Q8CDG3, Q8CF97, Q8K284, Q8N302, Q8TEW8

Diamond homologs: B1AZ99, F4K3M6, P22856, Q5T2D3, Q9D8C3, B2RRE7, P10383, Q01804, Q08BW0, Q2YDU3, Q3U2S4, Q640H3, Q6GL44, Q7ZX21, Q96G74, Q9NP73, Q9XIP2, Q0V869, Q5ZIP6, Q6IE21, Q7L8S5, Q8LBW2, Q196X6

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

47 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	36
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1726 predictions. Top by Δscore:

Variant	Effect	Δscore
1:19882664:G:GT	donor_gain	1.0000
1:19882691:C:G	donor_gain	1.0000
1:19882712:C:G	donor_gain	1.0000
1:19882718:G:GT	donor_gain	1.0000
1:19882718:G:T	donor_gain	1.0000
1:19890529:GCATG:G	donor_gain	1.0000
1:19894363:T:G	acceptor_gain	1.0000
1:19894366:A:AG	acceptor_gain	1.0000
1:19894367:G:GA	acceptor_gain	1.0000
1:19897531:T:A	acceptor_gain	1.0000
1:19897535:TACA:T	acceptor_loss	1.0000
1:19897537:C:G	acceptor_gain	1.0000
1:19897537:CA:C	acceptor_loss	1.0000
1:19897538:A:AG	acceptor_gain	1.0000
1:19897538:A:T	acceptor_loss	1.0000
1:19897539:G:GT	acceptor_gain	1.0000
1:19897539:GA:G	acceptor_gain	1.0000
1:19897539:GAT:G	acceptor_gain	1.0000
1:19897539:GATT:G	acceptor_gain	1.0000
1:19897539:GATTC:G	acceptor_gain	1.0000
1:19897658:CGGAT:C	donor_gain	1.0000
1:19897659:GGAT:G	donor_gain	1.0000
1:19897659:GGATG:G	donor_gain	1.0000
1:19897660:GAT:G	donor_gain	1.0000
1:19897660:GATG:G	donor_gain	1.0000
1:19897661:AT:A	donor_gain	1.0000
1:19897663:G:GA	donor_loss	1.0000
1:19897663:G:GG	donor_gain	1.0000
1:19897664:T:G	donor_loss	1.0000
1:19897665:G:GG	donor_loss	1.0000

AlphaMissense

2655 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:19890389:T:C	C76R	1.000
1:19890395:T:C	F78L	1.000
1:19890396:T:C	F78S	1.000
1:19890397:C:A	F78L	1.000
1:19890397:C:G	F78L	1.000
1:19890450:G:C	R96T	1.000
1:19890500:T:C	F113L	1.000
1:19890502:T:A	F113L	1.000
1:19890502:T:G	F113L	1.000
1:19894394:T:C	F133L	1.000
1:19894396:T:A	F133L	1.000
1:19894396:T:G	F133L	1.000
1:19897613:T:A	V186D	1.000
1:19882707:T:C	L65P	0.999
1:19882731:A:T	D73V	0.999
1:19882733:G:T	G74C	0.999
1:19882734:G:A	G74D	0.999
1:19882734:G:T	G74V	0.999
1:19890388:T:A	N75K	0.999
1:19890388:T:G	N75K	0.999
1:19890390:G:A	C76Y	0.999
1:19890391:C:G	C76W	0.999
1:19890395:T:A	F78I	0.999
1:19890395:T:G	F78V	0.999
1:19890396:T:G	F78C	0.999
1:19890399:G:C	R79T	0.999
1:19890399:G:T	R79I	0.999
1:19890402:C:A	A80D	0.999
1:19890411:A:C	D83A	0.999
1:19890451:A:C	R96S	0.999

dbSNP variants (sampled 300 via entrez): RS1000159544 (1:19887390 C>G,T), RS1000250696 (1:19904142 G>A,T), RS1000379440 (1:19893955 C>T), RS1000401320 (1:19912351 C>A,T), RS1000456536 (1:19880899 G>A), RS1000469321 (1:19898451 G>A), RS1000494449 (1:19892541 A>G), RS1000574528 (1:19904936 A>G), RS1000590298 (1:19899548 A>G), RS1000642814 (1:19906347 C>G,T), RS1000759610 (1:19910422 A>G), RS1000778701 (1:19910955 C>T), RS1000839287 (1:19912132 T>A,G), RS1000985739 (1:19904521 A>G), RS1001055611 (1:19881236 A>C)

Disease associations

OMIM: gene MIM:611758 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

11 associations (top):

Study	Trait	p-value
GCST000311_4	Ulcerative colitis	5.000000e-13
GCST000527_9	Ulcerative colitis	2.000000e-11
GCST000623_1	Ulcerative colitis	9.000000e-22
GCST000624_1	Ulcerative colitis	3.000000e-08
GCST000624_11	Ulcerative colitis	2.000000e-10
GCST000624_21	Ulcerative colitis	2.000000e-21
GCST001938_4	Ulcerative colitis	7.000000e-09
GCST004131_40	Inflammatory bowel disease	1.000000e-19
GCST004133_1	Ulcerative colitis	3.000000e-42
GCST008260_12	Group IIA secretory phospholipase A2 levels in individuals with elevated hsCRP	2.000000e-11
GCST008260_13	Group IIA secretory phospholipase A2 levels in individuals with elevated hsCRP	1.000000e-09

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL4630831 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

22 total (human), top 22 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Acetaminophen	increases expression	2
GSK-J4	increases expression	1
FR900359	increases phosphorylation	1
triphenyl phosphate	affects expression	1
alpha-pinene	affects cotreatment, decreases expression, increases abundance	1
pirinixic acid	affects binding, decreases expression, increases activity	1
methylparaben	increases expression	1
sodium arsenite	increases expression	1
methacrylaldehyde	affects cotreatment, decreases expression, increases abundance	1
Resveratrol	affects cotreatment, increases expression	1
Acrolein	affects cotreatment, decreases expression, increases abundance	1
Air Pollutants	affects cotreatment, decreases expression, increases abundance	1
Cisplatin	decreases expression	1
Methyl Methanesulfonate	increases expression	1
Ozone	affects cotreatment, decreases expression, increases abundance	1
Plant Extracts	affects cotreatment, increases expression	1
Tetrachlorodibenzodioxin	increases expression	1
Tobacco Smoke Pollution	decreases expression	1
Urethane	increases expression	1
Valproic Acid	increases expression	1
Aflatoxin B1	affects expression	1
Volatile Organic Compounds	affects cotreatment, decreases expression	1

ChEMBL screening assays

4 unique, capped per target: 4 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4605997	Binding	Inhibition of human GST-tagged OTUD3 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis	Discovery of Potent, Selective, and Orally Bioavailable Inhibitors of USP7 with In Vivo Antitumor Activity. — J Med Chem

Cellosaurus cell lines

2 cell lines: 2 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_TB72	HAP1 OTUD3 (-) 1	Cancer cell line	Male
CVCL_TB73	HAP1 OTUD3 (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.