OVGP1

Summary

OVGP1 (oviductal glycoprotein 1, HGNC:8524) is a protein-coding gene on chromosome 1p13.2, encoding Oviduct-specific glycoprotein (Q12889). Binds to oocyte zona pellucida in vivo.

This gene encodes a large, carbohydrate-rich, epithelial glycoprotein with numerous O-glycosylation sites located within threonine, serine, and proline-rich tandem repeats. The gene is similar to members of the mucin and the glycosyl hydrolase 18 gene families. Regulation of expression may be estrogen-dependent. Gene expression and protein secretion occur during late follicular development through early cleavage-stage embryonic development. The protein is secreted from non-ciliated oviductal epithelial cells and associates with ovulated oocytes, blastomeres, and spermatozoan acrosomal regions.

Source: NCBI Gene 5016 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 126 total
• MANE Select transcript: NM_002557

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:8524
Approved symbol	OVGP1
Name	oviductal glycoprotein 1
Location	1p13.2
Locus type	gene with protein product
Status	Approved
Aliases	CHIT5, OGP
Ensembl gene	ENSG00000085465
Ensembl biotype	protein_coding
OMIM	603578
Entrez	5016

Gene structure

Transcript identifiers

Ensembl transcripts: 5 — 3 protein_coding, 2 protein_coding_CDS_not_defined

ENST00000369732, ENST00000481495, ENST00000494622, ENST00000943841, ENST00000943842

RefSeq mRNA: 1 — MANE Select: NM_002557 NM_002557

CCDS: CCDS834

Canonical transcript exons

ENST00000369732 — 11 exons

Exon	Start	End
ENSE00000783695	111421276	111421461
ENSE00000783699	111425383	111425439
ENSE00000913271	111421565	111421673
ENSE00000913274	111423543	111423708
ENSE00001747757	111427062	111427091
ENSE00001845101	111414319	111415344
ENSE00002291162	111427697	111427735
ENSE00003511060	111426437	111426641
ENSE00003563991	111419610	111419726
ENSE00003592926	111422927	111423051
ENSE00003665849	111416323	111416458

Expression profiles

Bgee: expression breadth ubiquitous, 214 present calls, max score 99.93.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.1264 / max 18.3438, expressed in 52 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
13812	0.0942	42
13814	0.0247	6
13813	0.0074	3

Top tissues by expression

275 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
right uterine tube	UBERON:0001302	99.93	gold quality
type B pancreatic cell	CL:0000169	91.31	gold quality
olfactory bulb	UBERON:0002264	90.86	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	90.27	gold quality
fallopian tube	UBERON:0003889	89.97	gold quality
left uterine tube	UBERON:0001303	87.54	gold quality
oviduct epithelium	UBERON:0004804	85.70	gold quality
cervix squamous epithelium	UBERON:0006922	83.91	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	82.90	gold quality
cerebellar hemisphere	UBERON:0002245	82.82	gold quality
right hemisphere of cerebellum	UBERON:0014890	82.80	gold quality
pancreatic ductal cell	CL:0002079	82.78	silver quality
cerebellar cortex	UBERON:0002129	82.62	gold quality
skin of leg	UBERON:0001511	81.79	gold quality
superficial temporal artery	UBERON:0001614	81.75	gold quality
nasal cavity epithelium	UBERON:0005384	81.51	silver quality
mucosa of stomach	UBERON:0001199	81.26	gold quality
cerebellum	UBERON:0002037	81.26	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	81.10	gold quality
right frontal lobe	UBERON:0002810	80.43	gold quality
diaphragm	UBERON:0001103	80.16	gold quality
right lobe of liver	UBERON:0001114	79.89	gold quality
skin of abdomen	UBERON:0001416	79.41	gold quality
epithelial cell of pancreas	CL:0000083	79.26	gold quality
zone of skin	UBERON:0000014	79.03	gold quality
left testis	UBERON:0004533	78.98	gold quality
Brodmann (1909) area 9	UBERON:0013540	78.64	gold quality
nasal cavity mucosa	UBERON:0001826	78.40	gold quality
cervix epithelium	UBERON:0004801	78.29	gold quality
germinal epithelium of ovary	UBERON:0001304	78.20	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	4.19

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

11 targeting OVGP1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3148	99.97	75.06	6478
HSA-MIR-3688-3P	99.97	72.02	2834
HSA-MIR-6124	99.87	69.78	3551
HSA-MIR-582-5P	99.47	70.79	2635
HSA-MIR-6770-5P	98.97	66.76	1853
HSA-MIR-29B-1-5P	98.86	68.35	1364
HSA-MIR-581	98.39	67.42	835
HSA-MIR-668-3P	96.18	65.80	673
HSA-MIR-10396A-3P	93.99	62.06	94
HSA-MIR-10396B-3P	93.99	62.06	94
HSA-MIR-5587-3P	82.90	60.79	138

Literature-anchored findings (GeneRIF, showing 10)

• cloning and characterization of the promoter and gene expression regulation in the oviduct (human oviduct-specific glycoprotein, HuOPG) (PMID:11818519)
• The production and function of oviductin at different stages of human reproductive cycle is not well known. Its highest expression at the time of ovulation is consistent with a supportive role in fertilization and early embryo development. (PMID:12503889)
• Gain of oviduct-specific glycoprotein is associated with the development of endometrial hyperplasia and endometrial cancer (PMID:15585630)
• The presence of oviductin mRNA and protein has been determined in OE-E6/E7, an immortalized human oviductal epithelial cell line. (PMID:16209999)
• Results suggests the binding of OGP to human sperm mediates through MYH9. (PMID:16567366)
• Reflux laryngitis is associated with down-regulation of mucin gene expression. (PMID:18834073)
• OVGP1 seems to detect a different spectrum of ovarian epithelial cancers than other markers and thus should be a useful adjunct for more accurate detection, particularly of early serous ovarian cancers and mucinous carcinomas. (PMID:20130498)
• Decreased gene expression of MUC9 was found from colonic biopsies in patients with active and remission ulcerative colitis. (PMID:22028245)
• OVGP1 is specifically induced in the luminal epithelium at the time of embryo implantation where it regulates receptivity-related genes and aids in trophoblast adhesion. (PMID:29968069)
• The present study revealed that one of the major rHuOVGP1-enhanced pY proteins could be AKAP3 of the FS and that rHuOVGP1 is capable of binding to human ZP and its presence in the medium results in an increase in sperm-zona binding. Supplement of rHuOVGP1 in in vitro fertilization media could be beneficial for enhancement of the fertilizing ability of human sperm. (PMID:31254143)

Cross-species orthologs

42 orthologs

Organism	Symbol	Gene ID
mus_musculus	Ovgp1	ENSMUSG00000074340
drosophila_melanogaster	Idgf6	FBGN0013763
drosophila_melanogaster	Idgf3	FBGN0020414
drosophila_melanogaster	Idgf2	FBGN0020415
drosophila_melanogaster	Idgf1	FBGN0020416
drosophila_melanogaster	Cht4	FBGN0022700
drosophila_melanogaster	Idgf4	FBGN0026415
drosophila_melanogaster	Cht8	FBGN0034580
drosophila_melanogaster	Cht9	FBGN0034582
drosophila_melanogaster	Cht7	FBGN0035398
drosophila_melanogaster	Cht12	FBGN0050293
drosophila_melanogaster	Cda4	FBGN0052499
drosophila_melanogaster	Idgf5	FBGN0064237
drosophila_melanogaster	Cht10	FBGN0250907
caenorhabditis_elegans		WBGENE00000503
caenorhabditis_elegans		WBGENE00007465
caenorhabditis_elegans		WBGENE00007466
caenorhabditis_elegans		WBGENE00007467
caenorhabditis_elegans		WBGENE00007469
caenorhabditis_elegans		WBGENE00007470
caenorhabditis_elegans		WBGENE00007471
caenorhabditis_elegans		WBGENE00007472
caenorhabditis_elegans		WBGENE00007473
caenorhabditis_elegans		WBGENE00010799
caenorhabditis_elegans		WBGENE00010945
caenorhabditis_elegans		WBGENE00011157
caenorhabditis_elegans		WBGENE00011158
caenorhabditis_elegans		WBGENE00011159
caenorhabditis_elegans		WBGENE00011161
caenorhabditis_elegans		WBGENE00011162
caenorhabditis_elegans		WBGENE00011164
caenorhabditis_elegans		WBGENE00011166
caenorhabditis_elegans		WBGENE00011167
caenorhabditis_elegans		WBGENE00011170
caenorhabditis_elegans		WBGENE00011846
caenorhabditis_elegans		WBGENE00014162
caenorhabditis_elegans		WBGENE00016665
caenorhabditis_elegans		WBGENE00017233
caenorhabditis_elegans		WBGENE00020141
caenorhabditis_elegans		WBGENE00020407
caenorhabditis_elegans		WBGENE00044546
caenorhabditis_elegans		WBGENE00044807

Paralogs (6): CHI3L2 (ENSG00000064886), CTBS (ENSG00000117151), CHI3L1 (ENSG00000133048), CHIT1 (ENSG00000133063), CHIA (ENSG00000134216), CHID1 (ENSG00000177830)

Protein

Protein identifiers

Oviduct-specific glycoprotein — Q12889 (reviewed: Q12889)

Alternative names: Estrogen-dependent oviduct protein, Mucin-9, Oviductal glycoprotein, Oviductin

All UniProt accessions (1): Q12889

UniProt curated annotations — full annotation on UniProt →

Function. Binds to oocyte zona pellucida in vivo. May play a role in the fertilization process and/or early embryonic development.

Subcellular location. Cytoplasmic vesicle. Secretory vesicle.

Tissue specificity. Oviduct.

Similarity. Belongs to the glycosyl hydrolase 18 family.

RefSeq proteins (1): NP_002548* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001223	Glyco_hydro18_cat	Domain
IPR011583	Chitinase_II/V-like_cat	Domain
IPR017853	GH_hydrolase_sf	Homologous_superfamily
IPR029070	Chitinase_insertion_sf	Homologous_superfamily
IPR050314	Glycosyl_Hydrlase_18	Family

Pfam: PF00704

UniProt features (29 total): sequence variant 9, binding site 5, glycosylation site 5, region of interest 3, compositionally biased region 2, signal peptide 1, chain 1, disulfide bond 1, domain 1, sequence conflict 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q12889-F1	70.20	0.55

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (5): 142; 211–214; 355; 71–72; 98–101

Disulfide bonds (1): 26–51

Glycosylation sites (5): 402, 441, 580, 596, 648

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-2534343	Interaction With Cumulus Cells And The Zona Pellucida

MSigDB gene sets: 129 (showing top): GOBP_SINGLE_FERTILIZATION, FXR_IR1_Q6, GOBP_NEGATIVE_REGULATION_OF_REPRODUCTIVE_PROCESS, GOBP_MACROMOLECULE_CATABOLIC_PROCESS, GOBP_CARBOHYDRATE_DERIVATIVE_CATABOLIC_PROCESS, SHIPP_DLBCL_CURED_VS_FATAL_DN, GOBP_CARBOHYDRATE_DERIVATIVE_METABOLIC_PROCESS, YOKOE_CANCER_TESTIS_ANTIGENS, GOBP_SPERM_EGG_RECOGNITION, GOBP_AMINOGLYCAN_METABOLIC_PROCESS, GOBP_CARBOHYDRATE_METABOLIC_PROCESS, GOBP_REGULATION_OF_REPRODUCTIVE_PROCESS, GOBP_AMINO_SUGAR_CATABOLIC_PROCESS, GOBP_AMINO_SUGAR_METABOLIC_PROCESS, GOBP_MULTI_MULTICELLULAR_ORGANISM_PROCESS

GO Biological Process (5): carbohydrate metabolic process (GO:0005975), chitin catabolic process (GO:0006032), single fertilization (GO:0007338), female pregnancy (GO:0007565), negative regulation of binding of sperm to zona pellucida (GO:2000360)

GO Molecular Function (2): chitinase activity (GO:0004568), chitin binding (GO:0008061)

GO Cellular Component (7): extracellular region (GO:0005576), cytosol (GO:0005829), microtubule cytoskeleton (GO:0015630), transport vesicle (GO:0030133), egg coat (GO:0035805), perivitelline space (GO:0098595), cytoplasmic vesicle (GO:0031410)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
Fertilization	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
cytoplasm	2
primary metabolic process	1
aminoglycan catabolic process	1
chitin metabolic process	1
glucosamine-containing compound catabolic process	1
fertilization	1
multi-organism reproductive process	1
multi-multicellular organism process	1
binding of sperm to zona pellucida	1
negative regulation of cellular process	1
negative regulation of reproductive process	1
regulation of binding of sperm to zona pellucida	1
hydrolase activity, hydrolyzing O-glycosyl compounds	1
carbohydrate derivative binding	1
cytoskeleton	1
endomembrane system	1
cytoplasmic vesicle	1
specialized extracellular matrix	1
extracellular region	1
intracellular vesicle	1

Protein interactions and networks

STRING

776 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
OVGP1	GP6	Q9HCN6	767
OVGP1	ITGA2	P17301	652
OVGP1	MUC3A	Q02505	644
OVGP1	MUC15	Q8N387	638
OVGP1	MUC7	Q8TAX7	631
OVGP1	MUC12	Q9UKN1	628
OVGP1	MUC20	Q8N307	608
OVGP1	MUC13	Q9H3R2	605
OVGP1	MUC6	Q6W4X9	597
OVGP1	COL3A1	P02461	592
OVGP1	MUC16	Q8WXI7	582
OVGP1	MYH9	P35579	579
OVGP1	CHID1	Q9BWS9	565
OVGP1	MUC2	Q02817	561
OVGP1	MUC5B	Q9HC84	552

IntAct

4 interactions, top by confidence:

A	B	Type	Score
ASL	OVGP1	psi-mi:“MI:0915”(physical association)	0.370
OVGP1	AKAP10	psi-mi:“MI:0914”(association)	0.350
RASA4	OVGP1	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (6): OVGP1 (Affinity Capture-RNA), AKAP10 (Affinity Capture-MS), PEX6 (Affinity Capture-MS), OVGP1 (Positive Genetic), OVGP1 (Negative Genetic), OVGP1 (Two-hybrid)

ESM2 similar proteins: A4H238, A4H239, A4H241, A4H244, B3EWZ7, O14798, O35930, O46598, O54947, O88799, P03200, P03224, P09256, P16721, P28968, P38565, P97881, Q09550, Q12889, Q14242, Q28256, Q28983, Q2EG98, Q30KK3, Q5FVR0, Q5HZW7, Q5QGU6, Q5QNS5, Q5W9T8, Q5XI99, Q60401, Q60557, Q62010, Q62170, Q6R8J2, Q6S6W0, Q6SWB9, Q6U7R4, Q8N687, Q8R183

Diamond homologs: A0A072UR65, A0A072VEP0, A0A1B1J8Z2, O81862, P36222, P36718, Q12889, Q28990, Q29411, Q5RBP6, Q60557, Q61362, Q9WTV1, U5N4E3, A6N6J0, E9ERT9, O35744, P29030, P36362, P48827, Q13231, Q15782, Q28042, Q28542, Q62010, Q6RY07, Q91XA9, Q91Z98, Q95M17, Q9BZP6, Q9W092, Q9W5U2, A0A0W0FPC8, A0A385MIS5, O14456, P20533, P30922, P36909, Q6TMG6, Q8SPQ0

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

126 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	94
Likely benign	9
Benign	8

Top pathogenic / likely-pathogenic (0)

SpliceAI

1386 predictions. Top by Δscore:

Variant	Effect	Δscore
1:111422925:ACCTT:A	donor_gain	1.0000
1:111422926:C:A	donor_loss	1.0000
1:111422926:CCTT:C	donor_gain	1.0000
1:111422926:CCTTC:C	donor_gain	1.0000
1:111425377:TCTCA:T	donor_loss	1.0000
1:111425378:CTCAC:C	donor_loss	1.0000
1:111425379:TCAC:T	donor_loss	1.0000
1:111425380:CAC:C	donor_loss	1.0000
1:111425381:A:C	donor_loss	1.0000
1:111425382:C:T	donor_loss	1.0000
1:111425514:T:C	acceptor_gain	1.0000
1:111425514:T:TC	acceptor_gain	1.0000
1:111425519:C:CT	acceptor_gain	1.0000
1:111425520:A:C	acceptor_gain	1.0000
1:111425521:T:C	acceptor_gain	1.0000
1:111425521:T:TC	acceptor_gain	1.0000
1:111426436:CCT:C	donor_gain	1.0000
1:111426440:T:A	donor_gain	1.0000
1:111426478:AT:A	donor_gain	1.0000
1:111415342:ACTCT:A	acceptor_gain	0.9900
1:111415343:CT:C	acceptor_gain	0.9900
1:111415345:C:CC	acceptor_gain	0.9900
1:111417507:CCAAA:C	acceptor_gain	0.9900
1:111417508:CAAA:C	acceptor_gain	0.9900
1:111419722:CAAAT:C	acceptor_gain	0.9900
1:111421314:T:TA	donor_gain	0.9900
1:111421315:C:A	donor_gain	0.9900
1:111421553:T:A	donor_gain	0.9900
1:111421558:T:A	donor_gain	0.9900
1:111421674:C:CC	acceptor_gain	0.9900

AlphaMissense

4420 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:111419622:G:C	S336R	0.988
1:111419622:G:T	S336R	0.988
1:111419624:T:G	S336R	0.988
1:111419610:C:A	K340N	0.987
1:111419610:C:G	K340N	0.987
1:111421601:G:C	S227R	0.986
1:111421601:G:T	S227R	0.986
1:111421603:T:G	S227R	0.986
1:111421378:A:C	F267L	0.983
1:111421378:A:T	F267L	0.983
1:111421380:A:G	F267L	0.983
1:111419646:C:A	W328C	0.982
1:111419646:C:G	W328C	0.982
1:111419648:A:G	W328R	0.981
1:111419648:A:T	W328R	0.981
1:111421443:A:G	W246R	0.976
1:111421443:A:T	W246R	0.976
1:111426545:C:G	C51S	0.976
1:111426546:A:T	C51S	0.976
1:111416401:C:G	D360H	0.975
1:111421404:C:A	G259W	0.973
1:111421404:C:G	G259R	0.970
1:111421404:C:T	G259R	0.970
1:111426621:A:G	C26R	0.969
1:111426544:G:C	C51W	0.967
1:111426619:A:C	C26W	0.966
1:111426620:C:G	C26S	0.966
1:111426621:A:T	C26S	0.966
1:111426523:A:C	F58L	0.965
1:111426523:A:T	F58L	0.965

dbSNP variants (sampled 300 via entrez): RS1000226065 (1:111413947 C>A), RS1000553772 (1:111426644 G>A), RS1001132132 (1:111420367 C>T), RS1001562634 (1:111424688 C>T), RS1001941493 (1:111426157 C>A,T), RS1003138443 (1:111423393 A>T), RS1003234025 (1:111418560 A>G), RS1003291454 (1:111418357 T>C), RS1003556741 (1:111420708 C>T), RS1003734093 (1:111424992 T>C), RS1003895884 (1:111425783 T>G), RS1004017610 (1:111427856 A>C,G), RS1004064134 (1:111425695 C>T), RS1004089683 (1:111414894 C>T), RS1004126062 (1:111419175 A>G)

Disease associations

OMIM: gene MIM:603578 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST007059_13	Response to antidepressants (symptom improvement)	2.000000e-06

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

25 total (human), top 25 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
aristolochic acid I	increases expression	1
methyleugenol	increases expression	1
pirinixic acid	increases activity, affects binding, decreases expression	1
potassium chromate(VI)	affects cotreatment, increases expression	1
epigallocatechin gallate	affects cotreatment, increases expression	1
CGP 52608	affects binding, increases reaction	1
abrine	increases expression	1
jinfukang	affects cotreatment, decreases expression	1
(+)-JQ1 compound	increases expression	1
Sunitinib	increases expression	1
Zoledronic Acid	increases expression	1
Leflunomide	increases expression	1
Benzo(a)pyrene	affects methylation	1
Cisplatin	affects cotreatment, decreases expression	1
Clozapine	increases expression	1
Estradiol	increases expression, decreases reaction	1
N-Nitrosopyrrolidine	increases expression	1
Progesterone	decreases reaction, increases expression	1
Selenium	affects cotreatment, decreases expression, increases expression	1
Silicon Dioxide	decreases expression	1
Tretinoin	decreases expression	1
Tunicamycin	increases expression	1
Valproic Acid	decreases expression, decreases methylation	1
Vitamin E	affects cotreatment, decreases expression	1
Cadmium Chloride	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.