PAGE5

Summary

PAGE5 (PAGE family member 5, HGNC:29992) is a protein-coding gene on chromosome Xp11.21, encoding P antigen family member 5 (Q96GU1).

This gene is a member of family of proteins that are expressed in a variety of tumors and in some fetal and reproductive tissues. The encoded protein may protect cells from programmed cell death. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found.

Source: NCBI Gene 90737 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 22 total
• MANE Select transcript: NM_001013435

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:29992
Approved symbol	PAGE5
Name	PAGE family member 5
Location	Xp11.21
Locus type	gene with protein product
Status	Approved
Aliases	PAGE-5, CT16.1, CT16.2
Ensembl gene	ENSG00000158639
Ensembl biotype	protein_coding
OMIM	301009
Entrez	90737

Gene structure

Transcript identifiers

Ensembl transcripts: 3 — 3 protein_coding

ENST00000289619, ENST00000374952, ENST00000374955

RefSeq mRNA: 2 — MANE Select: NM_001013435 NM_001013435, NM_130467

CCDS: CCDS14368, CCDS35306

Canonical transcript exons

ENST00000374955 — 5 exons

Exon	Start	End
ENSE00001465242	55223987	55224108
ENSE00001465250	55220346	55220452
ENSE00001735618	55222621	55222746
ENSE00001799330	55221767	55221875
ENSE00003477164	55221375	55221463

Expression profiles

Bgee: expression breadth broad, 91 present calls, max score 93.71.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 3.4146 / max 350.6766, expressed in 140 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
196460	3.2079	133
196462	0.1120	37
196461	0.0946	34

Top tissues by expression

217 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	93.71	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	90.91	gold quality
left testis	UBERON:0004533	87.72	gold quality
right testis	UBERON:0004534	87.28	gold quality
testis	UBERON:0000473	86.13	gold quality
sperm	CL:0000019	73.41	gold quality
secondary oocyte	CL:0000655	63.70	gold quality
lateral nuclear group of thalamus	UBERON:0002736	59.90	gold quality
right lobe of liver	UBERON:0001114	58.39	gold quality
vena cava	UBERON:0004087	56.91	gold quality
parotid gland	UBERON:0001831	56.14	gold quality
dorsal plus ventral thalamus	UBERON:0001897	55.46	gold quality
amniotic fluid	UBERON:0000173	55.44	gold quality
saphenous vein	UBERON:0007318	55.44	gold quality
pericardium	UBERON:0002407	55.22	gold quality
nipple	UBERON:0002030	55.16	gold quality
subthalamic nucleus	UBERON:0001906	54.93	gold quality
layer of synovial tissue	UBERON:0007616	54.92	gold quality
urethra	UBERON:0000057	54.90	gold quality
trigeminal ganglion	UBERON:0001675	54.85	gold quality
pylorus	UBERON:0001166	54.76	gold quality
synovial joint	UBERON:0002217	54.73	gold quality
substantia nigra pars reticulata	UBERON:0001966	54.68	gold quality
thymus	UBERON:0002370	54.67	gold quality
substantia nigra pars compacta	UBERON:0001965	54.65	gold quality
dorsal root ganglion	UBERON:0000044	54.60	gold quality
ventral tegmental area	UBERON:0002691	54.52	gold quality
trachea	UBERON:0003126	54.48	gold quality
epithelium of nasopharynx	UBERON:0001951	54.47	gold quality
penis	UBERON:0000989	54.41	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-GEOD-134144	yes	28.76
E-ANND-3	no	2.29

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

20 targeting PAGE5, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-5582-3P	99.86	72.48	4221
HSA-MIR-579-3P	99.86	71.66	3628
HSA-MIR-548AR-3P	99.85	71.26	3889
HSA-MIR-664B-3P	99.84	71.65	3590
HSA-MIR-548AZ-3P	99.82	70.56	3549
HSA-MIR-548BC	99.82	70.61	3524
HSA-MIR-548E-3P	99.82	70.59	3514
HSA-MIR-548F-3P	99.82	70.59	3540
HSA-MIR-3180-5P	99.82	69.12	2422
HSA-MIR-548A-3P	99.76	70.58	3524
HSA-MIR-29B-2-5P	99.67	68.98	1726
HSA-MIR-6512-3P	99.65	66.07	1468
HSA-MIR-6720-5P	99.65	66.22	1459
HSA-MIR-548G-3P	99.48	68.67	2159
HSA-MIR-3123	99.47	67.15	2693
HSA-MIR-16-2-3P	99.29	70.60	1954
HSA-MIR-195-3P	99.29	70.61	1954
HSA-MIR-4477A	98.83	69.75	2952
HSA-MIR-4274	98.59	66.10	630
HSA-MIR-338-3P	98.14	67.38	1137

Literature-anchored findings (GeneRIF, showing 1)

• Results indicate that CT16 promotes the survival of melanoma cells and is therefore a potential target for future drug development. (PMID:23028975)

Cross-species orthologs

0 orthologs

Paralogs (22): PAGE1 (ENSG00000068985), PAGE4 (ENSG00000101951), XAGE2 (ENSG00000155622), XAGE3 (ENSG00000171402), XAGE5 (ENSG00000171405), GAGE2A (ENSG00000189064), PAGE3 (ENSG00000204279), XAGE1A (ENSG00000204379), XAGE1B (ENSG00000204382), GAGE1 (ENSG00000205777), GAGE12G (ENSG00000215269), GAGE10 (ENSG00000215274), GAGE12E (ENSG00000216649), GAGE12J (ENSG00000224659), GAGE12H (ENSG00000224902), GAGE12D (ENSG00000227488), PAGE2 (ENSG00000234068), GAGE12F (ENSG00000236362), GAGE12C (ENSG00000237671), PAGE2B (ENSG00000238269), GAGE13 (ENSG00000274274), GAGE2E (ENSG00000275113)

Protein

Protein identifiers

P antigen family member 5 — Q96GU1 (reviewed: Q96GU1)

Alternative names: Cancer/testis antigen 16.1, G antigen family E member 1, Prostate-associated gene 5 protein

All UniProt accessions (2): Q96GU1, Q5JUL1

UniProt curated annotations — full annotation on UniProt →

Similarity. Belongs to the GAGE family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q96GU1-1	1	yes
Q96GU1-2	2

RefSeq proteins (2): NP_001013453, NP_569734 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR008625	GAGE_fam	Family
IPR031320	GAGE	Domain

Pfam: PF05831

UniProt features (8 total): region of interest 2, compositionally biased region 2, modified residue 2, chain 1, splice variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q96GU1-F1	61.28	0.00

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (2): 113, 116

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 15 (showing top): ACEVEDO_METHYLATED_IN_LIVER_CANCER_DN, chrXp11, RATTENBACHER_BOUND_BY_CELF1, ZWANG_TRANSIENTLY_UP_BY_2ND_EGF_PULSE_ONLY, ZNF507_TARGET_GENES, ZNF766_TARGET_GENES, MIR5582_3P, MIR4477A, MIR338_3P, MIR4274, GSE18791_CTRL_VS_NEWCASTLE_VIRUS_DC_1H_DN, PULVER_FOREY_PERTURB_ACCUMULATION_G1_S, GSE2770_IL12_ACT_VS_ACT_CD4_TCELL_6H_UP, GSE21670_IL6_VS_TGFB_AND_IL6_TREATED_CD4_TCELL_UP, GSE21927_SPLEEN_VS_TUMOR_MONOCYTE_C57BL6_DN

GO Biological Process (0):

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (0):

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
binding	1

Protein interactions and networks

STRING

286 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
PAGE5	PRSS50	Q9UI38	841
PAGE5	SPA17	Q15506	720
PAGE5	SAGE1	Q9NXZ1	720
PAGE5	LIPI	Q6XZB0	665
PAGE5	PER2	O15055	621
PAGE5	MAGEC2	Q9UBF1	621
PAGE5	ADAM2	P78326	610
PAGE5	BMAL1	O00327	583
PAGE5	CRY1	Q16526	541
PAGE5	MAGEC1	O60732	506
PAGE5	CLOCK	O15516	480
PAGE5	XAGE5	Q8WWM1	476
PAGE5	NR1D1	P20393	475
PAGE5	CRY2	Q49AN0	475
PAGE5	ACRBP	Q8NEB7	447

IntAct

13 interactions, top by confidence:

A	B	Type	Score
MEOX2	PAGE5	psi-mi:“MI:0915”(physical association)	0.560
ERF	TBL1X	psi-mi:“MI:0914”(association)	0.530
POT1	PAGE5	psi-mi:“MI:0915”(physical association)	0.510
PAGE2	PAGE5	psi-mi:“MI:0915”(physical association)	0.400
ZNF460	ZNF320	psi-mi:“MI:0914”(association)	0.350
KRT83	OFD1	psi-mi:“MI:0914”(association)	0.350
PRR15	TNNC2	psi-mi:“MI:0914”(association)	0.350
FAM107B	MAP9	psi-mi:“MI:0914”(association)	0.350
SPATA2L	DCAF10	psi-mi:“MI:0914”(association)	0.350
POT1	PAGE5	psi-mi:“MI:0915”(physical association)	0.000
PAGE5	MEOX2	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (12): PAGE5 (Proximity Label-MS), PAGE5 (Two-hybrid), PAGE5 (Affinity Capture-MS), PAGE5 (Affinity Capture-MS), PAGE5 (Affinity Capture-MS), PAGE5 (Affinity Capture-MS), PAGE5 (Affinity Capture-MS), PAGE5 (Affinity Capture-MS), PAGE5 (Affinity Capture-MS), PAGE5 (Affinity Capture-MS), PAGE5 (Affinity Capture-MS), PAGE5 (Two-hybrid)

ESM2 similar proteins: A1L429, A6NDE8, A6NER3, A6NGK3, O41801, O55777, O60829, O75459, O76087, P03204, P04611, P06937, P0CL80, P0CL81, P0CL82, P0DSO3, P0DTW1, P18804, P22421, P86478, P86479, P86480, P86481, P86496, Q13066, Q13069, Q13070, Q2T9P9, Q3KST0, Q4V321, Q4V326, Q5JRK9, Q5JUK9, Q6AY45, Q6I7R5, Q6IPX3, Q6NT46, Q7Z2X7, Q8AIH8, Q8CCT4

Diamond homologs: O75459, Q5JRK9, Q5JUK9, Q7Z2X7, Q8WTP9, Q8WWM1, Q96GT9, Q96GU1, A6NGK3

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

22 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	17
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

555 predictions. Top by Δscore:

Variant	Effect	Δscore
X:55221373:A:AG	acceptor_gain	1.0000
X:55221374:G:GG	acceptor_gain	1.0000
X:55221460:GATT:G	donor_gain	1.0000
X:55221464:G:GG	donor_gain	1.0000
X:55221882:G:GT	donor_gain	1.0000
X:55221884:A:T	donor_gain	1.0000
X:55221909:G:GA	donor_gain	1.0000
X:55222618:AAG:A	acceptor_gain	1.0000
X:55222619:A:G	acceptor_gain	1.0000
X:55222743:GCAG:G	donor_gain	1.0000
X:55222744:CAGG:C	donor_loss	1.0000
X:55222745:AGGT:A	donor_loss	1.0000
X:55222746:GG:G	donor_loss	1.0000
X:55222747:G:GC	donor_loss	1.0000
X:55222748:T:A	donor_loss	1.0000
X:55220449:GCCG:G	donor_gain	0.9900
X:55221369:TTTCA:T	acceptor_loss	0.9900
X:55221370:TTCAG:T	acceptor_loss	0.9900
X:55221371:TCAGT:T	acceptor_loss	0.9900
X:55221372:CAGTG:C	acceptor_gain	0.9900
X:55221373:A:AC	acceptor_loss	0.9900
X:55221373:AGT:A	acceptor_gain	0.9900
X:55221373:AGTGA:A	acceptor_gain	0.9900
X:55221374:G:A	acceptor_loss	0.9900
X:55221374:GT:G	acceptor_gain	0.9900
X:55221374:GTG:G	acceptor_gain	0.9900
X:55221374:GTGA:G	acceptor_gain	0.9900
X:55221374:GTGAG:G	acceptor_gain	0.9900
X:55221462:TT:T	donor_gain	0.9900
X:55221748:C:G	acceptor_gain	0.9900

AlphaMissense

709 scored. Top likely-pathogenic:

dbSNP variants (sampled 300 via entrez): RS1000290960 (X:55222856 A>G), RS1001364645 (X:55223123 A>G), RS1006110822 (X:55224286 A>G), RS1006367825 (X:55221172 T>A), RS1007322454 (X:55222276 TTAA>T), RS1008941643 (X:55224152 T>C), RS1010964281 (X:55220816 G>T), RS1011845276 (X:55218768 T>C), RS1014079644 (X:55222439 G>A), RS1014825922 (X:55224318 T>C), RS1016042543 (X:55220705 G>A,C,T), RS1016121669 (X:55218397 G>A), RS1016838651 (X:55221177 T>C), RS1017556955 (X:55221391 G>T), RS1018948643 (X:55224165 A>C,G)

Disease associations

OMIM: gene MIM:301009 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST006661_51	Male-pattern baldness	5.000000e-10

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

9 total (human), top 9 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
zinc chromate	increases abundance, increases expression	1
chromium hexavalent ion	increases abundance, increases expression	1
CGP 52608	affects binding, increases reaction	1
clothianidin	decreases expression	1
Resveratrol	affects cotreatment, decreases expression	1
Decitabine	increases expression	1
Benzo(a)pyrene	affects methylation, decreases methylation	1
Plant Extracts	affects cotreatment, decreases expression	1
Silicon Dioxide	increases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.