PAIP1

Summary

PAIP1 (poly(A) binding protein interacting protein 1, HGNC:16945) is a protein-coding gene on chromosome 5p12, encoding Polyadenylate-binding protein-interacting protein 1 (Q9H074). Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.

The protein encoded by this gene interacts with poly(A)-binding protein and with the cap-binding complex eIF4A. It is involved in translational initiation and protein biosynthesis. Overexpression of this gene in COS7 cells stimulates translation. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified.

Source: NCBI Gene 10605 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 68 total
• Druggable target: yes
• MANE Select transcript: NM_006451

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:16945
Approved symbol	PAIP1
Name	poly(A) binding protein interacting protein 1
Location	5p12
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000172239
Ensembl biotype	protein_coding
OMIM	605184
Entrez	10605

Gene structure

Transcript identifiers

Ensembl transcripts: 15 — 14 protein_coding, 1 nonsense_mediated_decay

ENST00000306846, ENST00000338972, ENST00000436644, ENST00000504075, ENST00000504639, ENST00000508537, ENST00000511321, ENST00000514514, ENST00000514816, ENST00000515338, ENST00000869933, ENST00000869934, ENST00000869935, ENST00000869936, ENST00000929306

RefSeq mRNA: 3 — MANE Select: NM_006451 NM_006451, NM_182789, NM_183323

CCDS: CCDS3947, CCDS3948, CCDS47204

Canonical transcript exons

ENST00000306846 — 11 exons

Exon	Start	End
ENSE00001148824	43526267	43527469
ENSE00001175008	43538924	43539035
ENSE00001175015	43543004	43543116
ENSE00002222328	43556582	43557077
ENSE00003527789	43536819	43536944
ENSE00003557646	43529786	43529879
ENSE00003563297	43535534	43535640
ENSE00003626456	43533738	43533792
ENSE00003632834	43555830	43555999
ENSE00003694677	43534853	43534970
ENSE00003789643	43547728	43547913

Expression profiles

Bgee: expression breadth ubiquitous, 142 present calls, max score 98.53.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 33.6318 / max 262.9781, expressed in 1820 samples.

FANTOM5 promoters (7 alternative TSS)

Promoter ID	TPM avg	Samples expressed
61573	17.2883	1807
61570	8.8684	1788
61572	2.6953	1425
61574	2.1909	924
61569	1.7082	1200
61571	0.8188	586
61575	0.0619	31

Top tissues by expression

145 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
cortical plate	UBERON:0005343	98.53	gold quality
ventricular zone	UBERON:0003053	98.48	gold quality
embryo	UBERON:0000922	98.43	gold quality
ganglionic eminence	UBERON:0004023	98.43	gold quality
hindlimb stylopod muscle	UBERON:0004252	97.56	gold quality
gastrocnemius	UBERON:0001388	97.51	gold quality
muscle of leg	UBERON:0001383	97.48	gold quality
skeletal muscle organ	UBERON:0014892	97.34	gold quality
islet of Langerhans	UBERON:0000006	97.27	gold quality
smooth muscle tissue	UBERON:0001135	97.01	gold quality
heart left ventricle	UBERON:0002084	96.94	gold quality
endometrium	UBERON:0001295	96.83	gold quality
skeletal muscle tissue	UBERON:0001134	96.59	gold quality
muscle tissue	UBERON:0002385	96.44	gold quality
heart	UBERON:0000948	96.42	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	96.37	gold quality
spinal cord	UBERON:0002240	96.29	gold quality
gall bladder	UBERON:0002110	96.21	gold quality
popliteal artery	UBERON:0002250	96.16	gold quality
tibial artery	UBERON:0007610	96.16	gold quality
urinary bladder	UBERON:0001255	96.05	gold quality
muscle layer of sigmoid colon	UBERON:0035805	96.05	gold quality
right testis	UBERON:0004534	96.03	gold quality
left testis	UBERON:0004533	95.98	gold quality
placenta	UBERON:0001987	95.95	gold quality
rectum	UBERON:0001052	95.94	gold quality
calcaneal tendon	UBERON:0003701	95.91	gold quality
lower esophagus muscularis layer	UBERON:0035833	95.87	gold quality
lower esophagus	UBERON:0013473	95.86	gold quality
substantia nigra	UBERON:0002038	95.85	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	10.97

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

135 targeting PAIP1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-340-5P	100.00	72.50	4437
HSA-MIR-656-3P	100.00	72.15	2788
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-3646	100.00	73.56	5283
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-4789-3P	99.99	70.75	2484
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-3662	99.99	73.82	5684
HSA-MIR-548N	99.98	71.94	4170
HSA-MIR-4789-5P	99.98	70.76	2721
HSA-MIR-5696	99.98	72.36	4487
HSA-MIR-548P	99.98	72.25	3784
HSA-MIR-4775	99.98	75.00	6394
HSA-MIR-4803	99.98	71.99	3117
HSA-MIR-32-5P	99.98	75.21	1964
HSA-MIR-92A-3P	99.98	75.21	1960
HSA-MIR-92B-3P	99.98	75.25	1955
HSA-MIR-25-3P	99.98	74.60	1817
HSA-MIR-363-3P	99.98	74.72	1821
HSA-MIR-367-3P	99.98	74.83	1819
HSA-MIR-3148	99.97	75.06	6478
HSA-MIR-607	99.97	73.62	5593
HSA-MIR-507	99.97	70.11	1915
HSA-MIR-6793-5P	99.97	65.95	758
HSA-MIR-493-5P	99.96	72.47	2382
HSA-MIR-1468-3P	99.96	72.74	3797
HSA-MIR-590-3P	99.96	74.34	6478
HSA-MIR-548AT-5P	99.96	70.83	2666

Literature-anchored findings (GeneRIF, showing 15)

• Paip1 interacts with poly(A) binding protein through two independent binding motifs. (PMID:11997512)
• eIF3-Paip1 stabilizes the interaction between PABP and eIF4G, which brings about the circularization of the mRNA. (PMID:18725400)
• Paip1 is translational activator in 5’ cap-dependent translation by interacting with PABP & initiation factors eIF4A & eIF3. Crystallization & preliminary diffraction analysis of middle domain of Paip1 gives crystals that diffract to resolution of 2.2 A. (PMID:19851022)
• the crystal structure of the middle domain of Paip1 isoform 2 (Paip1M) as determined by single-wavelength anomalous dispersion phasing is reported. (PMID:21539810)
• The Paip1-eIF3 interaction is impaired by the mTORC1 inhibitors. (PMID:24396066)
• Data provide evidence that the stability of Paip1 can be regulated by ubiquitin-mediated degradation, thus highlighting the importance of WWP2 as a suppressor of translation. (PMID:25266661)
• our study demonstrated that Paip1 promotes the growth of breast cancers and could be a prognostic biomarker and therapeutic target (PMID:29258905)
• Results showed that mRNA and protein expression of PAIP1, a miRNA-340 target gene, were down-regulated in women with gestational diabetes. (PMID:29358694)
• Paip1 contributes to Pancreatic cancer progression and appears to be a valid prognostic factor of PC (PMID:30731074)
• These results indicated that PAIP1 and PAIP2 participate in translation termination and are important regulators of readthrough at the premature termination codon. (PMID:30992367)
• Paip1 was overexpressed in cervical cancer (CC) samples and associated with the adverse outcomes in patients with CC. Its knockdown induced inhibition of cell growth, G2/M arrest and apoptosis in CC cells. (PMID:31010277)
• High Paip1 Expression as a Potential Prognostic Marker in Hepatocellular Carcinoma. (PMID:32871777)
• The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. (PMID:33876849)
• Effect of PAIP1 on the metastatic potential and prognostic significance in oral squamous cell carcinoma. (PMID:35153296)
• YAP1-activated ZNF131 promotes hepatocellular carcinoma cell proliferation through transcriptional regulation of PAIP1. (PMID:38341068)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	paip1	ENSDARG00000042892
mus_musculus	Paip1	ENSMUSG00000025451
rattus_norvegicus	Paip1	ENSRNOG00000058580
drosophila_melanogaster	Paip1	FBGN0034181

Paralogs (2): MIF4GD (ENSG00000125457), CTIF (ENSG00000134030)

Protein

Protein identifiers

Polyadenylate-binding protein-interacting protein 1 — Q9H074 (reviewed: Q9H074)

All UniProt accessions (8): A0A1D5RMS4, D6R9H8, D6RD97, D6REB4, D6RJ02, D6RJF2, Q9H074, H0YA44

UniProt curated annotations — full annotation on UniProt →

Function. Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. (Microbial infection) Upon interaction with SARS coronavirus SARS-CoV NSP3 protein, plays an important role in viral protein synthesis.

Subunit / interactions. Interacts with the RRM1-RRM2 and C-terminus regions of PABPC1 in a 1:1 stoichiometry. Interacts with EIF4A. (Microbial infection) Interacts (via PAIP1M) with human SARS coronaviruses SARS-COV and SARS-COV-2 NSP3 protein (via SARS-unique domain); the interaction increases binding affinity with PABPC1.

Subcellular location. Cytoplasm.

Domain organisation. Only the PABPC1-interacting motif-1 (PAM1) stimulates translation initiation.

Isoforms (3)

UniProt ID	Names	Canonical?
Q9H074-1	1	yes
Q9H074-2	2
Q9H074-3	3

RefSeq proteins (3): NP_006442, NP_877590, NP_899152 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR003890	MIF4G-like_typ-3	Domain
IPR009818	PAM2_motif	Conserved_site
IPR016024	ARM-type_fold	Homologous_superfamily
IPR051367	mRNA_TranslReg/HistoneTransl	Family

Pfam: PF02854, PF07145

UniProt features (29 total): helix 12, region of interest 5, compositionally biased region 3, modified residue 2, splice variant 2, chain 1, domain 1, sequence conflict 1, strand 1, initiator methionine 1

Structure

Experimental structures (PDB)

4 structures.

PDB	Method	Resolution (Å)
3RK6	X-RAY DIFFRACTION	2
3NTW	X-RAY DIFFRACTION	2.6
6YXJ	X-RAY DIFFRACTION	3.5
1JH4	SOLUTION NMR

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9H074-F1	72.92	0.45

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (2): 21, 2

Function

Pathways and Gene Ontology

Reactome pathways

3 pathways

ID	Pathway
R-HSA-429947	Deadenylation of mRNA
R-HSA-9820841	M-decay: degradation of maternal mRNAs by maternally stored factors
R-HSA-9820865	Z-decay: degradation of maternal mRNAs by zygotically expressed factors

MSigDB gene sets: 216 (showing top): GOBP_CYTOPLASMIC_TRANSLATION, GOBP_REGULATION_OF_MRNA_CATABOLIC_PROCESS, GCM_ZNF198, DITTMER_PTHLH_TARGETS_UP, GOBP_MACROMOLECULE_CATABOLIC_PROCESS, GOBP_TRANSLATIONAL_INITIATION, GOBP_MODULATION_OF_PROCESS_OF_ANOTHER_ORGANISM, GOBP_HOST_MEDIATED_PERTURBATION_OF_VIRAL_PROCESS, RODWELL_AGING_KIDNEY_NO_BLOOD_DN, GOBP_TRANSLATION, GOBP_POST_TRANSCRIPTIONAL_REGULATION_OF_GENE_EXPRESSION, GOMF_TRANSLATION_REGULATOR_ACTIVITY, MILI_PSEUDOPODIA_HAPTOTAXIS_UP, GOBP_REGULATION_OF_CATABOLIC_PROCESS, GOBP_POSITIVE_REGULATION_OF_CATABOLIC_PROCESS

GO Biological Process (8): translational initiation (GO:0006413), regulation of translational initiation (GO:0006446), host-mediated activation of viral process (GO:0044794), mRNA stabilization (GO:0048255), CRD-mediated mRNA stabilization (GO:0070934), negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay (GO:1900152), positive regulation of cytoplasmic translation (GO:2000767), regulation of translation (GO:0006417)

GO Molecular Function (3): RNA binding (GO:0003723), translation activator activity (GO:0008494), protein binding (GO:0005515)

GO Cellular Component (3): cytoplasm (GO:0005737), cytosol (GO:0005829), mCRD-mediated mRNA stability complex (GO:0106002)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
Maternal to zygotic transition (MZT)	2
Deadenylation-dependent mRNA decay	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
translation	2
negative regulation of mRNA catabolic process	2
positive regulation of translation	2
cellular anatomical structure	2
formation of translation initiation ternary complex	1
metabolic process	1
translational initiation	1
regulation of translation	1
host-mediated perturbation of viral process	1
regulation of mRNA stability	1
RNA stabilization	1
mRNA stabilization	1
nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	1
regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	1
cytoplasmic translation	1
regulation of cytoplasmic translation	1
post-transcriptional regulation of gene expression	1
regulation of protein metabolic process	1
nucleic acid binding	1
translation regulator activity	1
binding	1
intracellular anatomical structure	1
cytoplasm	1
cytosol	1
protein-containing complex	1

Protein interactions and networks

STRING

2352 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
PAIP1	PABPC1	P11940	998
PAIP1	SYNCRIP	O60506	961
PAIP1	PAIP2	Q9BPZ3	940
PAIP1	HNRNPD	P07029	929
PAIP1	EIF4A1	P04765	925
PAIP1	EIF4A2	Q14240	925
PAIP1	EIF3G	O75821	907
PAIP1	EIF4E	P06730	769
PAIP1	CSDE1	O75534	753
PAIP1	FOS	P01100	726
PAIP1	PAIP2B	Q9ULR5	668
PAIP1	PABPC3	Q9H361	668
PAIP1	PABPC4	Q13310	650
PAIP1	RRM1	P23921	608
PAIP1	TMEM267	Q0VDI3	608

IntAct

80 interactions, top by confidence:

A	B	Type	Score
PAIP1	PABPC1	psi-mi:“MI:0914”(association)	0.970
PABPC1	PAIP1	psi-mi:“MI:0407”(direct interaction)	0.970
PABPC1	PAIP1	psi-mi:“MI:0915”(physical association)	0.970
PAIP1	PABPC1	psi-mi:“MI:0915”(physical association)	0.970
PAIP1	PABPC1	psi-mi:“MI:0407”(direct interaction)	0.970
PABPC1	PAIP2	psi-mi:“MI:0915”(physical association)	0.860
USP12	PHLPP1	psi-mi:“MI:0914”(association)	0.570
ELAVL2	IGF2BP3	psi-mi:“MI:0914”(association)	0.530
SNIP1	CASC3	psi-mi:“MI:0914”(association)	0.530
CELF5	CASC3	psi-mi:“MI:0914”(association)	0.530
PAIP2B	CASC3	psi-mi:“MI:0914”(association)	0.530
CDKN2AIP	MAGEB2	psi-mi:“MI:0914”(association)	0.530
IGF2BP3	PTCD1	psi-mi:“MI:0914”(association)	0.530
PAIP1	IGF2BP3	psi-mi:“MI:0914”(association)	0.530
PAIP1	Eif4a1	psi-mi:“MI:0915”(physical association)	0.510
Eif4a1	PAIP1	psi-mi:“MI:0915”(physical association)	0.510
PABPC1		psi-mi:“MI:0914”(association)	0.500

BioGRID (231): PAIP1 (Affinity Capture-Western), PABPC1 (Affinity Capture-Western), PAIP1 (Reconstituted Complex), WWP2 (Reconstituted Complex), PAIP1 (Biochemical Activity), PAIP1 (Affinity Capture-MS), PAIP1 (Affinity Capture-MS), PAIP1 (Affinity Capture-MS), PAIP1 (Affinity Capture-MS), PAIP1 (Affinity Capture-MS), PAIP1 (Affinity Capture-MS), PAIP1 (Affinity Capture-MS), MKRN2 (Affinity Capture-MS), STAU2 (Affinity Capture-MS), PABPC1L (Affinity Capture-MS)

ESM2 similar proteins: A0JPM9, A2AQ19, O43395, O75391, O75822, P04973, P09496, P29084, P29540, Q02614, Q0VCU8, Q13123, Q15650, Q2HJ41, Q2KIA6, Q2KJF9, Q3MHJ0, Q3UGC7, Q5BK07, Q5I0B5, Q5NVI3, Q5R5F1, Q5R8D1, Q5RAD5, Q5RE03, Q5ZJ85, Q5ZJ97, Q5ZK25, Q5ZKA4, Q66HG8, Q66JS6, Q6GMH0, Q6INR1, Q6P320, Q7SXU0, Q7SYJ9, Q7TNE3, Q8BM39, Q91WE2, Q922U1

Diamond homologs: Q7ZYB4, Q8VE62, Q9H074

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
WWP2	“down-regulates quantity by destabilization”	PAIP1	ubiquitination

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 69 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

GO biological processes:

GO term	Partners	Fold	FDR
negative regulation of translation	6	20.6×	2e-04
RNA processing	5	19.2×	8e-04
mRNA splicing, via spliceosome	7	11.2×	5e-04

Disease & clinical

Clinical variants and AI predictions

ClinVar

68 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	36
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1882 predictions. Top by Δscore:

Variant	Effect	Δscore
5:43527465:AGTAT:A	acceptor_gain	1.0000
5:43527466:GTAT:G	acceptor_gain	1.0000
5:43527467:TAT:T	acceptor_gain	1.0000
5:43527468:AT:A	acceptor_gain	1.0000
5:43527469:TCTG:T	acceptor_loss	1.0000
5:43527470:C:CA	acceptor_loss	1.0000
5:43527470:C:CC	acceptor_gain	1.0000
5:43527471:T:A	acceptor_loss	1.0000
5:43528134:A:AC	donor_gain	1.0000
5:43528135:C:CC	donor_gain	1.0000
5:43529779:AACTT:A	donor_loss	1.0000
5:43529780:ACTTA:A	donor_loss	1.0000
5:43529781:CTTAC:C	donor_loss	1.0000
5:43529783:TAC:T	donor_loss	1.0000
5:43529784:A:AC	donor_gain	1.0000
5:43529785:C:CA	donor_gain	1.0000
5:43529785:CG:C	donor_gain	1.0000
5:43529785:CGG:C	donor_gain	1.0000
5:43529785:CGGAT:C	donor_gain	1.0000
5:43529875:GTAAT:G	acceptor_gain	1.0000
5:43529876:TAAT:T	acceptor_gain	1.0000
5:43529877:AAT:A	acceptor_gain	1.0000
5:43529878:AT:A	acceptor_gain	1.0000
5:43529878:ATCT:A	acceptor_loss	1.0000
5:43529880:C:CC	acceptor_gain	1.0000
5:43529880:C:T	acceptor_loss	1.0000
5:43534848:CATA:C	donor_loss	1.0000
5:43534849:ATACC:A	donor_loss	1.0000
5:43534850:TACC:T	donor_loss	1.0000
5:43534851:A:T	donor_loss	1.0000

AlphaMissense

3114 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
5:43534919:C:A	W377C	1.000
5:43534919:C:G	W377C	1.000
5:43534921:A:G	W377R	1.000
5:43534921:A:T	W377R	1.000
5:43527409:A:C	F469L	0.999
5:43527409:A:T	F469L	0.999
5:43527411:A:G	F469L	0.999
5:43534935:A:G	L372P	0.999
5:43535633:C:T	G327E	0.999
5:43533755:A:G	F412S	0.998
5:43533777:A:C	Y405D	0.998
5:43533778:A:C	F404L	0.998
5:43533778:A:T	F404L	0.998
5:43533780:A:G	F404L	0.998
5:43534918:C:G	G378R	0.998
5:43534920:C:G	W377S	0.998
5:43535634:C:G	G327R	0.998
5:43535634:C:T	G327R	0.998
5:43536819:C:A	K324N	0.998
5:43536819:C:G	K324N	0.998
5:43538940:A:G	L277P	0.998
5:43555860:A:C	F135L	0.998
5:43555860:A:T	F135L	0.998
5:43555862:A:G	F135L	0.998
5:43527423:C:G	A465P	0.997
5:43533764:C:A	G409V	0.997
5:43533779:A:G	F404S	0.997
5:43533785:G:T	P402Q	0.997
5:43536823:A:G	L323S	0.997
5:43538928:A:G	L281P	0.997

dbSNP variants (sampled 300 via entrez): RS1000021506 (5:43527018 A>G), RS1000061555 (5:43534419 G>C), RS1000124278 (5:43541659 G>A,T), RS1000174906 (5:43543615 T>C), RS1000223636 (5:43541315 G>A,C), RS1000281930 (5:43547738 A>C), RS1000521462 (5:43531334 A>G,T), RS1000590501 (5:43545118 C>T), RS1000654611 (5:43553669 T>C), RS1000677473 (5:43557028 G>A,C), RS1000813142 (5:43559271 C>G,T), RS1000917454 (5:43528822 A>T), RS1000967411 (5:43540139 G>A), RS1001019613 (5:43540461 C>A,T), RS1001065041 (5:43536128 T>C)

Disease associations

OMIM: gene MIM:605184 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST003265_351	Post bronchodilator FEV1/FVC ratio in COPD	2.000000e-06
GCST003265_352	Post bronchodilator FEV1/FVC ratio in COPD	2.000000e-06
GCST003265_353	Post bronchodilator FEV1/FVC ratio in COPD	2.000000e-06
GCST003265_354	Post bronchodilator FEV1/FVC ratio in COPD	2.000000e-06

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0004713	FEV/FVC ratio

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067353 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

3 potent at pChembl≥5 of 4 total, top 3 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
8.49	Kd	3.242	nM	CHEMBL5653589
8.26	ED50	5.519	nM	CHEMBL5653589
5.09	Kd	8186	nM	CHEMBL3752910

PubChem BioAssay actives

2 with measured affinity, of 4 total; 2 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148932: Binding affinity to human PAIP1 incubated for 45 mins by Kinobead based pull down assay	kd	0.0032	uM
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148932: Binding affinity to human PAIP1 incubated for 45 mins by Kinobead based pull down assay	kd	8.1860	uM

CTD chemical–gene interactions

42 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	decreases expression, affects cotreatment, increases expression	3
trichostatin A	affects cotreatment, decreases expression	3
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, increases expression, decreases expression	2
Acetaminophen	decreases expression	2
Valproic Acid	decreases expression	2
aristolochic acid I	decreases expression	1
GSK-J4	increases expression	1
dicrotophos	decreases expression	1
methylmercuric chloride	decreases expression	1
zinc chloride	decreases expression	1
cobaltous chloride	increases expression	1
4-hydroxy-2-nonenal	decreases expression	1
deguelin	decreases expression	1
abrine	decreases expression	1
dorsomorphin	affects cotreatment, decreases expression	1
jinfukang	decreases expression	1
LDN 193189	affects cotreatment, increases expression	1
NSC 689534	affects binding, decreases expression	1
Amphotericin B	decreases expression	1
Atrazine	decreases expression	1
Benzo(a)pyrene	decreases methylation	1
Caffeine	affects phosphorylation	1
Cisplatin	decreases expression	1
Copper	affects binding, decreases expression	1
Coumestrol	decreases expression	1
Cyclophosphamide	decreases expression	1
Demecolcine	decreases expression	1
Dexamethasone	affects cotreatment, increases expression	1
Dietary Carbohydrates	decreases expression	1
Doxorubicin	decreases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5651974	Binding	Binding affinity to human PAIP1 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.