PAOX

Summary

PAOX (polyamine oxidase, HGNC:20837) is a protein-coding gene on chromosome 10q26.3, encoding Peroxisomal N(1)-acetyl-spermine/spermidine oxidase (Q6QHF9). Flavoenzyme which catalyzes the oxidation of N(1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion.

Enables polyamine oxidase activity. Involved in polyamine metabolic process and positive regulation of spermidine biosynthetic process. Predicted to be located in cytosol and peroxisomal matrix. Predicted to be active in cytoplasm. Implicated in lung non-small cell carcinoma.

Source: NCBI Gene 196743 — RefSeq curated summary.

At a glance

• GWAS associations: 5
• Clinical variants (ClinVar): 89 total
• Druggable target: yes
• MANE Select transcript: NM_152911

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:20837
Approved symbol	PAOX
Name	polyamine oxidase
Location	10q26.3
Locus type	gene with protein product
Status	Approved
Aliases	PAO
Ensembl gene	ENSG00000148832
Ensembl biotype	protein_coding
OMIM	615853
Entrez	196743

Gene structure

Transcript identifiers

Ensembl transcripts: 15 — 8 protein_coding, 4 nonsense_mediated_decay, 3 protein_coding_CDS_not_defined

ENST00000278060, ENST00000356306, ENST00000357296, ENST00000368535, ENST00000476834, ENST00000480071, ENST00000483211, ENST00000528127, ENST00000529585, ENST00000530555, ENST00000877115, ENST00000877116, ENST00000877117, ENST00000963865, ENST00000963866

RefSeq mRNA: 3 — MANE Select: NM_152911 NM_152911, NM_207127, NM_207128

CCDS: CCDS7682, CCDS7683, CCDS7684

Canonical transcript exons

ENST00000278060 — 7 exons

Exon	Start	End
ENSE00002164654	133379262	133379497
ENSE00003499305	133391312	133391694
ENSE00003537478	133389590	133389747
ENSE00003561236	133383960	133384212
ENSE00003566247	133388956	133389068
ENSE00003607381	133381460	133381659
ENSE00003667900	133379999	133380485

Expression profiles

Bgee: expression breadth ubiquitous, 216 present calls, max score 95.83.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 5.2132 / max 68.5220, expressed in 1604 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
107793	3.1302	1420
107794	2.0708	1014
107792	0.0123	3

Top tissues by expression

279 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
sperm	CL:0000019	95.83	gold quality
left testis	UBERON:0004533	93.72	gold quality
right testis	UBERON:0004534	93.54	gold quality
male germ cell	CL:0000015	93.30	gold quality
testis	UBERON:0000473	90.94	gold quality
lower esophagus mucosa	UBERON:0035834	86.33	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	86.11	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	85.07	gold quality
right lobe of liver	UBERON:0001114	85.02	gold quality
esophagus mucosa	UBERON:0002469	81.72	gold quality
mucosa of transverse colon	UBERON:0004991	79.74	gold quality
spleen	UBERON:0002106	78.68	gold quality
granulocyte	CL:0000094	78.47	gold quality
sural nerve	UBERON:0015488	78.17	gold quality
esophagus	UBERON:0001043	77.68	gold quality
cervix squamous epithelium	UBERON:0006922	77.23	gold quality
ventricular zone	UBERON:0003053	77.06	gold quality
small intestine Peyer’s patch	UBERON:0003454	76.79	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	76.76	gold quality
monocyte	CL:0000576	76.75	gold quality
leukocyte	CL:0000738	76.72	gold quality
mononuclear cell	CL:0000842	76.51	gold quality
parotid gland	UBERON:0001831	76.03	silver quality
small intestine	UBERON:0002108	75.42	gold quality
tendon of biceps brachii	UBERON:0008188	75.29	silver quality
ectocervix	UBERON:0012249	75.24	gold quality
liver	UBERON:0002107	74.98	gold quality
Brodmann (1909) area 9	UBERON:0013540	74.75	gold quality
spinal cord	UBERON:0002240	74.71	gold quality
prefrontal cortex	UBERON:0000451	74.37	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	5.10

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

9 targeting PAOX, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-7978	99.86	66.90	856
HSA-MIR-6745	99.74	65.33	1321
HSA-MIR-363-5P	99.46	64.51	1015
HSA-MIR-6770-5P	98.97	66.76	1853
HSA-MIR-6787-3P	97.75	66.17	1233
HSA-MIR-409-5P	97.31	68.07	364
HSA-MIR-215-3P	97.02	68.01	1209
HSA-MIR-6729-3P	96.91	66.79	703
HSA-MIR-1291	96.28	65.89	1224

Literature-anchored findings (GeneRIF, showing 6)

• SSAT and SMO(PAOh1) activities are the major mediators of the cellular response of breast tumor cells to polyamines while PAO plays little or no role in this response (PMID:16207710)
• polyamine oxidase degrades alpha-methylpolyamines in a stereospecific manner (PMID:16354669)
• PAO activity in children with type 1 diabetes mellitus was very high; higher blood HbA(1C) and MDA levels confirm the presence of oxidant stress demonstrate that PAO activity may participate in these circumstances (PMID:20405312)
• copy number gain in the polyamine oxidase (PAOX) gene locus was accompanied by a coordinated transcriptional up-regulation in Primary myelofibrosis patients (PMID:26547506)
• Gene polymorphisms of SFTPB rs7316, rs9752 and PAOX rs1046175 affect the diagnostic value of plasma Pro-SFTPB and DAS in Chinese Han non-small-cell lung cancer patients. (PMID:31016788)
• Polyamine Oxidase Expression Is Downregulated by 17beta-Estradiol via Estrogen Receptor 2 in Human MCF-7 Breast Cancer Cells. (PMID:35886868)

Cross-species orthologs

12 orthologs

Organism	Symbol	Gene ID
danio_rerio	paox	ENSDARG00000003380
danio_rerio	PAOX	ENSDARG00000109596
mus_musculus	Paox	ENSMUSG00000025464
rattus_norvegicus	Paox	ENSRNOG00000018838
drosophila_melanogaster	CG10561	FBGN0002036
drosophila_melanogaster	CG7737	FBGN0033584
drosophila_melanogaster	CG5653	FBGN0035943
drosophila_melanogaster	CG7460	FBGN0036749
drosophila_melanogaster	CG6034	FBGN0036750
drosophila_melanogaster	CG8032	FBGN0037606
caenorhabditis_elegans	spr-5	WBGENE00005010
caenorhabditis_elegans		WBGENE00011615

Paralogs (7): KDM1A (ENSG00000004487), MAOB (ENSG00000069535), SMOX (ENSG00000088826), IL4I1 (ENSG00000104951), PPOX (ENSG00000143224), KDM1B (ENSG00000165097), MAOA (ENSG00000189221)

Protein

Protein identifiers

Peroxisomal N(1)-acetyl-spermine/spermidine oxidase — Q6QHF9 (reviewed: Q6QHF9)

Alternative names: Polyamine oxidase

All UniProt accessions (1): Q6QHF9

UniProt curated annotations — full annotation on UniProt →

Function. Flavoenzyme which catalyzes the oxidation of N(1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N(1)-acetylspermidine to putrescine. Substrate specificity: N(1)-acetylspermine = N(1)-acetylspermidine > N(1),N(12)-diacylspermine » spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs.

Subunit / interactions. Monomer.

Subcellular location. Peroxisome. Cytoplasm.

Tissue specificity. Widely expressed. Not detected in spleen. Expressed at lower level in neoplastic tissues.

Cofactor. Binds 1 FAD per subunit.

Induction. By polyamine analogs.

Pathway. Amine and polyamine metabolism; spermine metabolism.

Miscellaneous. Oxidizes N(1)-acetylated polyamines on the exo-side of their N(4)-amino groups. Plant PAO oxidizes spermine on the endo-side of the N(4)-nitrogen. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Similarity. Belongs to the flavin monoamine oxidase family.

Isoforms (4)

UniProt ID	Names	Canonical?
Q6QHF9-2	1	yes
Q6QHF9-6	2
Q6QHF9-5	3
Q6QHF9-4	4

RefSeq proteins (3): NP_690875, NP_997010, NP_997011 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR002937	Amino_oxidase	Domain
IPR036188	FAD/NAD-bd_sf	Homologous_superfamily
IPR050281	Flavin_monoamine_oxidase	Family

Pfam: PF01593

Enzyme classification (BRENDA):

• EC 1.5.3.13 — N1-acetylpolyamine oxidase (BRENDA: 7 organisms, 77 substrates, 31 inhibitors, 53 Km, 31 kcat entries)

Substrate kinetics (BRENDA)

22 substrates with measured Km, best-characterized 15. Km ranges are aggregated across organisms/conditions.

Substrate	Km (mM)	Measurements
N1-ACETYLSPERMINE	0.0008–0.382	16
SPERMINE	0.0093–11	8
N1-ACETYLSPERMIDINE	0.0021–0.028	5
ALPHA-METHYLSPERMINE	0.011–0.019	4
N1-PHENYL-(R)-ALPHA-METHYLSPERMIDINE	0.0016–0.002	2
3-[4-(3-AMINOPROPOXY)BUTOXY]-N-BENZYLPROPAN-1-AM	0.06	1
ALPHA-METHYLSPERMIDINE	0.016	1
N-[(2R)-4-[(4-AMINOBUTYL)AMINO]BUTAN-2-YL]PYRIDI	0.0036	1
N-[(2S)-4-[(4-AMINOBUTYL)AMINO]BUTAN-2-YL]PYRIDI	0.0008	1
N1,N11-DIACETYLNORSPERMINE	0.0027	1
N1-(3-[[(THIOPHEN-2-YL)METHYL]AMINO]PROPYL)OCTAN	0.001	1
N1-BENZOYL-(R)-ALPHA-METHYLSPERMIDINE	0.0012	1
N1-BENZOYL-(S)-ALPHA-METHYLSPERMIDINE	0.0002	1
N1-BENZYLDODECANE-1,12-DIAMINE	0.025	1
N1-BENZYLSPERMINE	0.0002	1

Catalyzed reactions (Rhea), 3 shown:

• N(1)-acetylspermine + O2 + H2O = 3-acetamidopropanal + spermidine + H2O2 (RHEA:25800)
• N(1)-acetylspermidine + O2 + H2O = 3-acetamidopropanal + putrescine + H2O2 (RHEA:25812)
• N(1),N(12)-diacetylspermine + O2 + H2O = 3-acetamidopropanal + N(1)-acetylspermidine + H2O2 (RHEA:25868)

UniProt features (28 total): binding site 10, sequence conflict 9, splice variant 6, chain 1, short sequence motif 1, modified residue 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q6QHF9-F1	92.77	0.85

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (10): 472; 481–482; 24; 45; 53; 69–70; 72; 194; 247; 320

Post-translational modifications (1): 1

Function

Pathways and Gene Ontology

Reactome pathways

3 pathways

ID	Pathway
R-HSA-141334	PAOs oxidise polyamines to amines
R-HSA-351200	Interconversion of polyamines
R-HSA-9033241	Peroxisomal protein import

MSigDB gene sets: 97 (showing top): REACTOME_BIOLOGICAL_OXIDATIONS, GOBP_POSITIVE_REGULATION_OF_AMINE_METABOLIC_PROCESS, GOBP_REGULATION_OF_AMINE_METABOLIC_PROCESS, PID_INTEGRIN_A9B1_PATHWAY, GOBP_POLYAMINE_METABOLIC_PROCESS, BONOME_OVARIAN_CANCER_POOR_SURVIVAL_DN, GOMF_OXIDOREDUCTASE_ACTIVITY_ACTING_ON_THE_CH_NH_GROUP_OF_DONORS, IVANOVA_HEMATOPOIESIS_EARLY_PROGENITOR, DURCHDEWALD_SKIN_CARCINOGENESIS_UP, GOCC_MICROBODY, GOCC_MICROBODY_LUMEN, ZHANG_RESPONSE_TO_IKK_INHIBITOR_AND_TNF_UP, BRUINS_UVC_RESPONSE_VIA_TP53_GROUP_A, GOBP_POLYAMINE_BIOSYNTHETIC_PROCESS, GOBP_SPERMIDINE_METABOLIC_PROCESS

GO Biological Process (7): polyamine catabolic process (GO:0006598), putrescine biosynthetic process (GO:0009446), putrescine catabolic process (GO:0009447), spermidine catabolic process (GO:0046203), spermine catabolic process (GO:0046208), positive regulation of spermidine biosynthetic process (GO:1901307), spermine metabolic process (GO:0008215)

GO Molecular Function (3): polyamine oxidase activity (GO:0046592), N(1)-acetylpolyamine oxidase activity (GO:0052903), oxidoreductase activity (GO:0016491)

GO Cellular Component (4): cytoplasm (GO:0005737), peroxisomal matrix (GO:0005782), cytosol (GO:0005829), peroxisome (GO:0005777)

Reactome top-level categories

Rollup of top-3 pathways:

Category	Pathways
Amine Oxidase reactions	1
Metabolism of polyamines	1
Protein localization	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
polyamine catabolic process	3
polyamine metabolic process	2
putrescine metabolic process	2
cellular anatomical structure	2
biogenic amine catabolic process	1
polyamine biosynthetic process	1
spermidine metabolic process	1
spermine metabolic process	1
spermidine biosynthetic process	1
positive regulation of biosynthetic process	1
positive regulation of amine metabolic process	1
regulation of spermidine biosynthetic process	1
oxidoreductase activity, acting on the CH-NH group of donors, oxygen as acceptor	1
polyamine oxidase activity	1
catalytic activity	1
intracellular anatomical structure	1
peroxisome	1
microbody lumen	1
cytoplasm	1
microbody	1

Protein interactions and networks

STRING

523 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
PAOX	SAT1	P21673	987
PAOX	SAT2	Q96F10	895
PAOX	SMS	P52788	837
PAOX	SRM	P19623	821
PAOX	ODC1	P11926	810
PAOX	GLYATL1	Q969I3	762
PAOX	AOC1	P19801	712
PAOX	AZIN2	Q96A70	705
PAOX	NR0B1	P51843	631
PAOX	OTC	P00480	578
PAOX	OAZ2	O95190	570
PAOX	DHPS	P49366	527
PAOX	AGMAT	Q9BSE5	525
PAOX	PIPOX	Q9P0Z9	506
PAOX	PMF1	Q6P1K2	501

IntAct

0 interactions, top by confidence:

BioGRID (3): PAOX (Affinity Capture-RNA), PAOX (Affinity Capture-RNA), PAOX (Protein-peptide)

ESM2 similar proteins: A1L1C2, A3KNW0, A6H603, A6NFQ2, A6QLU7, A9ULG4, B1H1N7, E1BE10, E2RD63, O35405, O55230, O60294, O60906, O75771, O95479, P21709, P51839, P56201, Q0V8L6, Q149M9, Q1JPJ9, Q28DT3, Q2KJJ8, Q2TBP8, Q4R583, Q5FVH2, Q5R4Y7, Q5XIA3, Q60750, Q643R3, Q6NVG1, Q6QHF9, Q80XS7, Q865R1, Q8BG07, Q8BYR1, Q8C0L6, Q8CFX1, Q8IV08, Q8N0W3

Diamond homologs: A0A024BTN9, A0A1B1PF34, A0A2U8QPE6, A6MFL0, A8QL51, A8QL52, A8QL58, B0VXW0, B5AR80, B5U6Y8, C0HJE7, F4JLS1, F8S0Z5, G8XQX1, J7H670, K9N7B7, L7WC64, O07855, O09046, O24164, O60341, O93364, O96566, P05410, P06617, P0C2D5, P0CC17, P0DI84, P0DPE9, P17054, P19643, P21397, P21685, P22871, P27338, P54978, P56560, P56601, P56742, P58027

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

89 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	66
Likely benign	16
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1782 predictions. Top by Δscore:

Variant	Effect	Δscore
10:133380348:G:GT	donor_gain	1.0000
10:133380426:G:GA	donor_gain	1.0000
10:133380444:G:GT	donor_gain	1.0000
10:133380444:G:T	donor_gain	1.0000
10:133380482:C:CG	donor_gain	1.0000
10:133380482:C:G	donor_gain	1.0000
10:133380486:G:GG	donor_gain	1.0000
10:133381458:AG:A	acceptor_gain	1.0000
10:133381459:GG:G	acceptor_gain	1.0000
10:133381658:AGGTA:A	donor_loss	1.0000
10:133381660:G:GA	donor_loss	1.0000
10:133381660:G:GG	donor_gain	1.0000
10:133381661:T:G	donor_loss	1.0000
10:133384177:G:GT	donor_gain	1.0000
10:133388952:GCA:G	acceptor_loss	1.0000
10:133388953:CAG:C	acceptor_loss	1.0000
10:133389064:GACAG:G	donor_gain	1.0000
10:133389066:CAGG:C	donor_loss	1.0000
10:133389069:G:GG	donor_gain	1.0000
10:133389069:G:T	donor_loss	1.0000
10:133389070:T:G	donor_loss	1.0000
10:133380392:GCTGT:G	donor_gain	0.9900
10:133380395:GT:G	donor_gain	0.9900
10:133380425:T:TA	donor_gain	0.9900
10:133381456:CTA:C	acceptor_loss	0.9900
10:133381457:TA:T	acceptor_loss	0.9900
10:133381458:A:C	acceptor_loss	0.9900
10:133381458:AGG:A	acceptor_gain	0.9900
10:133381459:G:GC	acceptor_loss	0.9900
10:133381459:GGG:G	acceptor_gain	0.9900

AlphaMissense

3297 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
10:133389667:A:C	S438R	0.995
10:133389669:C:A	S438R	0.995
10:133389669:C:G	S438R	0.995
10:133388997:C:T	S388F	0.994
10:133380051:C:A	N78K	0.991
10:133380051:C:G	N78K	0.991
10:133380029:T:C	I71T	0.990
10:133389634:T:A	W427R	0.990
10:133389634:T:C	W427R	0.990
10:133391334:A:T	E472V	0.990
10:133381639:T:A	V283D	0.989
10:133380058:T:C	F81L	0.987
10:133380060:C:A	F81L	0.987
10:133380060:C:G	F81L	0.987
10:133384070:T:C	F327L	0.987
10:133384072:T:A	F327L	0.987
10:133384072:T:G	F327L	0.987
10:133391328:C:A	A470E	0.987
10:133391369:G:T	G484W	0.986
10:133388978:T:C	F382L	0.985
10:133388980:C:A	F382L	0.985
10:133388980:C:G	F382L	0.985
10:133388997:C:A	S388Y	0.984
10:133389636:G:C	W427C	0.984
10:133389636:G:T	W427C	0.984
10:133391325:T:C	F469S	0.984
10:133384056:A:T	K322I	0.983
10:133391385:G:A	G489E	0.983
10:133381540:T:A	I250N	0.982
10:133384057:A:C	K322N	0.982

dbSNP variants (sampled 300 via entrez): RS1000112512 (10:133387415 G>A), RS1000427962 (10:133385444 C>T), RS1000779390 (10:133385129 C>T), RS1001002699 (10:133385862 C>A,G,T), RS1001056362 (10:133385622 C>T), RS1001399673 (10:133379678 A>C), RS1001526590 (10:133384890 C>T), RS1001861056 (10:133379408 G>A,C), RS1002039100 (10:133379460 C>T), RS1002056303 (10:133384836 G>C), RS1002116056 (10:133389659 G>A,C,T), RS1002346094 (10:133385823 T>C), RS1002358563 (10:133380505 C>G), RS1002463962 (10:133388693 T>C), RS1002822982 (10:133380796 G>A)

Disease associations

OMIM: gene MIM:615853 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

5 associations (top):

Study	Trait	p-value
GCST009733_127	Urinary metabolite levels in chronic kidney disease	1.000000e-14
GCST009733_46	Urinary metabolite levels in chronic kidney disease	7.000000e-12
GCST009735_11	Urinary metabolite modules (eigenmetabolites) in chronic kidney disease	9.000000e-17
GCST012020_202	Serum metabolite levels	1.000000e-16
GCST012020_203	Serum metabolite levels	8.000000e-30

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0005116	urinary metabolite measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL2105 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

11 potent at pChembl≥5 of 14 total, top 11 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.05	Ki	90	nM	CHEMBL417844
7.02	Ki	96	nM	CHEMBL176596
7.00	Ki	100	nM	CHEMBL367753
6.75	Ki	180	nM	CHEMBL173782
6.52	Ki	300	nM	CHEMBL293698
6.20	Ki	630	nM	CHEMBL175345
6.16	Ki	700	nM	CHEMBL59622
6.10	IC50	790	nM	CHEMBL5094181
6.00	Ki	1000	nM	CHEMBL175307
5.68	IC50	2100	nM	CHEMBL5092747
5.66	Ki	2200	nM	CHEMBL176843

PubChem BioAssay actives

10 with measured affinity, of 83 total; 10 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
N,N’-bis(buta-2,3-dienyl)butane-1,4-diamine	162582: Inhibitory activity measured as apparent dissociation constant against Polyamine oxidase (PAO) from pig liver	ki	0.0900	uM
7-[3-(dimethylamino)propylamino]heptan-2-one	144352: Ability to inhibit the deacetylation of [acetyl-3H]-N8-Acetylspermidine deacetylase in rat liver.	ki	0.0960	uM
7-[3-(diethylamino)propylamino]heptan-2-one	144352: Ability to inhibit the deacetylation of [acetyl-3H]-N8-Acetylspermidine deacetylase in rat liver.	ki	0.1000	uM
Chlorhexidine	1803253: Enzyme Inhibition Assay from Article 10.3109/14756366.2011.650691: “Inhibition of acetylpolyamine and spermine oxidases by the polyamine analogue chlorhexidine.”	ki	0.1000	uM
7-(3-aminopropylamino)heptan-2-one	144352: Ability to inhibit the deacetylation of [acetyl-3H]-N8-Acetylspermidine deacetylase in rat liver.	ki	0.1800	uM
N’-buta-2,3-dienyl-N-methylbutane-1,4-diamine	162582: Inhibitory activity measured as apparent dissociation constant against Polyamine oxidase (PAO) from pig liver	ki	0.3000	uM
7-(4-methylpiperazin-1-yl)heptan-2-one	144352: Ability to inhibit the deacetylation of [acetyl-3H]-N8-Acetylspermidine deacetylase in rat liver.	ki	0.6300	uM
N’-buta-2,3-dienylbutane-1,4-diamine	162582: Inhibitory activity measured as apparent dissociation constant against Polyamine oxidase (PAO) from pig liver	ki	0.7000	uM
7-(3-morpholin-4-ylpropylamino)heptan-2-one	144352: Ability to inhibit the deacetylation of [acetyl-3H]-N8-Acetylspermidine deacetylase in rat liver.	ki	1.0000	uM
7-aminoheptan-2-one	144349: Inhibition of deacetylation of [acetyl-3H]-N8-Acetylspermidine deacetylase in rat liver	ki	2.2000	uM

CTD chemical–gene interactions

25 total (human), top 25 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Benzo(a)pyrene	decreases expression, increases methylation	2
Smoke	increases abundance, increases expression, decreases expression	2
Cyclosporine	decreases expression, decreases methylation	2
GSK-J4	decreases expression	1
afuresertib	increases expression	1
methylmercuric chloride	decreases expression	1
sulforaphane	decreases expression	1
tris(1,3-dichloro-2-propyl)phosphate	decreases expression	1
di-n-butylphosphoric acid	affects expression	1
jinfukang	affects cotreatment, increases expression	1
Sunitinib	increases expression	1
Arsenic Trioxide	increases expression	1
Air Pollutants	increases abundance, increases expression	1
Arsenic	affects methylation	1
Cisplatin	affects cotreatment, increases expression	1
Coal	increases expression, increases abundance	1
Diazinon	increases methylation	1
Folic Acid	decreases expression	1
Selenium	affects cotreatment, decreases expression	1
Silicon Dioxide	decreases expression	1
Testosterone	decreases expression	1
Urethane	decreases expression	1
Valproic Acid	increases methylation	1
Vitamin E	decreases expression, affects cotreatment	1
Aflatoxin B1	decreases expression	1

ChEMBL screening assays

16 unique, capped per target: 12 binding, 4 admet

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL2185104	Binding	Activity of APAO in human DU145 cells assessed per ug DNA at 100 uM after 72 hrs in presence of serum amine oxidase inhibitor aminoguanidine (RVb = 662 +/- 48 pmol/h per ug DNA)	The use of novel C-methylated spermidine derivatives to investigate the regulation of polyamine metabolism. — J Med Chem
CHEMBL2185553	ADMET	Metabolic stability of the compound assessed as human recombinant APAO-mediated compound degradation at 0.5 to 1 mM after 5 to 60 mins	The use of novel C-methylated spermidine derivatives to investigate the regulation of polyamine metabolism. — J Med Chem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.