PPIAL4F

Summary

PPIAL4F (peptidylprolyl isomerase A like 4F, HGNC:33999) is a protein-coding gene on chromosome 1q21.1, encoding Peptidyl-prolyl cis-trans isomerase A-like 4F (P0DN26). PPIases accelerate the folding of proteins.

Predicted to enable cyclosporin A binding activity and peptidyl-prolyl cis-trans isomerase activity. Predicted to be involved in protein folding. Predicted to be active in cytoplasm.

Source: NCBI Gene 728945 — RefSeq curated summary.

At a glance

• MANE Select transcript: NM_001164262

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:33999
Approved symbol	PPIAL4F
Name	peptidylprolyl isomerase A like 4F
Location	1q21.1
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000279782
Ensembl biotype	protein_coding
Entrez	728945

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000581138

RefSeq mRNA: 1 — MANE Select: NM_001164262 NM_001164262

CCDS: CCDS76202

Canonical transcript exons

ENST00000369222 — 0 exons

Expression profiles

Bgee: expression breadth ubiquitous, 102 present calls, max score 85.50.

Top tissues by expression

125 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	85.50	gold quality
granulocyte	CL:0000094	58.96	gold quality
sural nerve	UBERON:0015488	53.77	silver quality
mucosa of transverse colon	UBERON:0004991	53.46	gold quality
right uterine tube	UBERON:0001302	52.26	gold quality
blood	UBERON:0000178	48.21	gold quality
cerebellum	UBERON:0002037	48.09	gold quality
cerebellar cortex	UBERON:0002129	48.03	gold quality
cerebellar hemisphere	UBERON:0002245	47.41	gold quality
caudate nucleus	UBERON:0001873	47.38	gold quality
lower esophagus mucosa	UBERON:0035834	46.82	silver quality
nucleus accumbens	UBERON:0001882	45.84	gold quality
anterior cingulate cortex	UBERON:0009835	45.02	gold quality
putamen	UBERON:0001874	44.46	gold quality
right hemisphere of cerebellum	UBERON:0014890	43.60	gold quality
bone marrow	UBERON:0002371	43.21	silver quality
spleen	UBERON:0002106	43.04	gold quality
stromal cell of endometrium	CL:0002255	42.74	silver quality
ventricular zone	UBERON:0003053	41.65	gold quality
bone marrow cell	CL:0002092	41.43	gold quality
amygdala	UBERON:0001876	41.25	gold quality
brain	UBERON:0000955	41.22	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	41.19	gold quality
temporal lobe	UBERON:0001871	41.09	gold quality
Ammon’s horn	UBERON:0001954	40.58	gold quality
Brodmann (1909) area 9	UBERON:0013540	40.41	gold quality
hypothalamus	UBERON:0001898	39.93	gold quality
leukocyte	CL:0000738	39.56	silver quality
cerebral cortex	UBERON:0000956	39.15	gold quality
tonsil	UBERON:0002372	38.98	silver quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	1.49

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

9 targeting PPIAL4F, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-1252-5P	100.00	69.80	2774
HSA-MIR-4802-3P	99.72	70.13	1273
HSA-MIR-3202	99.66	67.70	2737
HSA-MIR-548V	99.29	69.47	1157
HSA-MIR-6830-5P	99.01	68.73	1884
HSA-MIR-4456	97.50	64.88	1678
HSA-MIR-3622B-5P	94.62	64.58	835
HSA-MIR-6750-5P	93.94	66.68	797
HSA-MIR-6822-5P	93.94	66.34	812

Cross-species orthologs

10 orthologs

Organism	Symbol	Gene ID
danio_rerio	ppifa	ENSDARG00000007409
danio_rerio	ppiaa	ENSDARG00000009212
danio_rerio	ppiab	ENSDARG00000103994
drosophila_melanogaster	Cyp40	FBGN0036020
drosophila_melanogaster	Moca-cyp	FBGN0039581
caenorhabditis_elegans		WBGENE00000877
caenorhabditis_elegans		WBGENE00000878
caenorhabditis_elegans		WBGENE00000879
caenorhabditis_elegans		WBGENE00000883
caenorhabditis_elegans		WBGENE00000885

Paralogs (22): PPIE (ENSG00000084072), PPIL2 (ENSG00000100023), PPIF (ENSG00000108179), PPWD1 (ENSG00000113593), NKTR (ENSG00000114857), PPIL4 (ENSG00000131013), PPIL1 (ENSG00000137168), PPIG (ENSG00000138398), CWC27 (ENSG00000153015), PPIB (ENSG00000166794), PPIC (ENSG00000168938), PPID (ENSG00000171497), PPIH (ENSG00000171960), PPIL6 (ENSG00000185250), PPIA (ENSG00000196262), PPIAL4G (ENSG00000236334), PPIL3 (ENSG00000240344), PPIAL4A (ENSG00000263353), PPIAL4H (ENSG00000270339), PPIAL4E (ENSG00000271567), PPIAL4C (ENSG00000288867), PPIAL4D (ENSG00000289549)

Protein

Protein identifiers

Peptidyl-prolyl cis-trans isomerase A-like 4F — P0DN26 (reviewed: P0DN26)

All UniProt accessions (1): P0DN26

UniProt curated annotations — full annotation on UniProt →

Function. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Subcellular location. Cytoplasm.

Miscellaneous. It is one of six related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 1.

Similarity. Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.

RefSeq proteins (1): NP_001157734* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR002130	Cyclophilin-type_PPIase_dom	Domain
IPR020892	Cyclophilin-type_PPIase_CS	Conserved_site
IPR024936	Cyclophilin-type_PPIase	Family
IPR029000	Cyclophilin-like_dom_sf	Homologous_superfamily

Pfam: PF00160

Catalyzed reactions (Rhea), 1 shown:

• [protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0) (RHEA:16237)

UniProt features (2 total): chain 1, domain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P0DN26-F1	96.65	0.98

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 10 (showing top): GOBP_PROTEIN_MATURATION, GOBP_PROTEIN_FOLDING, chr1q21, GOMF_CIS_TRANS_ISOMERASE_ACTIVITY, GOMF_AMIDE_BINDING, GOMF_ISOMERASE_ACTIVITY, GOMF_CYCLOSPORIN_A_BINDING, MIR548V, MIR6750_5P, MIR6822_5P

GO Biological Process (2): protein folding (GO:0006457), protein peptidyl-prolyl isomerization (GO:0000413)

GO Molecular Function (3): peptidyl-prolyl cis-trans isomerase activity (GO:0003755), cyclosporin A binding (GO:0016018), isomerase activity (GO:0016853)

GO Cellular Component (1): cytoplasm (GO:0005737)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular process	1
protein maturation	1
peptidyl-proline modification	1
cis-trans isomerase activity	1
catalytic activity, acting on a protein	1
binding	1
catalytic activity	1
intracellular anatomical structure	1
cellular anatomical structure	1

Protein interactions and networks

STRING

0 interactions, top by confidence (×1000):

IntAct

2 interactions, top by confidence:

A	B	Type	Score
PPIAL4F	PNN	psi-mi:“MI:0915”(physical association)	0.400

ESM2 similar proteins: A0A075B759, A0A075B767, A0A0B4J2A2, F5H284, O00060, O43447, O74729, O94273, P0C1I0, P0C1I3, P0C1I5, P0C1I8, P0CP82, P0CP83, P0DN26, P0DN37, P17742, P23285, P24525, P25719, P52010, P52018, P62940, P84343, Q0P5D0, Q0ZQK7, Q0ZQK8, Q0ZQL0, Q0ZQL1, Q26516, Q26551, Q26565, Q27774, Q2TZ33, Q38867, Q38900, Q42406, Q4IPH4, Q4P6X6, Q4WCM6

Diamond homologs: A0A075B759, A0A075B767, A0A0B4J2A2, A0A0R0H9T5, A4FV72, D4AY02, F5H284, H2QII6, O00060, O49886, O93826, P0C1H7, P0C1H8, P0C1I2, P0C1I7, P0C1I8, P0C1I9, P0CP78, P0CP79, P0DN26, P0DN37, P10111, P10255, P14088, P14832, P14851, P17742, P18253, P21568, P21569, P22011, P24367, P24525, P25007, P25719, P29117, P30404, P30405, P34790, P34791

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

0 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	0
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

273 predictions. Top by Δscore:

Variant	Effect	Δscore
1:144593068:A:T	donor_gain	0.7700
1:144593076:G:GT	donor_gain	0.7700
1:144593164:TGAC:T	donor_gain	0.7600
1:144593611:T:G	donor_gain	0.7200
1:144592982:G:A	donor_gain	0.7000
1:144593118:GGTT:G	donor_gain	0.7000
1:144593119:GTT:G	donor_gain	0.7000
1:144593120:TTT:T	donor_gain	0.7000
1:144593165:GACA:G	donor_gain	0.6900
1:144593166:ACAA:A	donor_gain	0.6900
1:144593095:C:G	donor_gain	0.6800
1:144592977:C:A	donor_gain	0.6700
1:144592929:CAGGG:C	donor_gain	0.6600
1:144593092:T:TA	donor_gain	0.6200
1:144593297:AGT:A	donor_gain	0.6100
1:144593093:T:A	donor_gain	0.6000
1:144593135:G:GA	donor_gain	0.6000
1:144593297:A:AC	donor_gain	0.6000
1:144593312:G:A	donor_gain	0.6000
1:144593072:A:T	donor_gain	0.5900
1:144593090:GGT:G	donor_gain	0.5800
1:144593303:C:CT	donor_gain	0.5800
1:144593054:GCTC:G	donor_gain	0.5700
1:144593091:G:T	donor_gain	0.5700
1:144593106:G:GT	donor_gain	0.5700
1:144593270:TCCC:T	donor_gain	0.5700
1:144593616:T:G	donor_gain	0.5600
1:144593089:GG:G	donor_gain	0.5500
1:144593042:G:GT	donor_gain	0.5400
1:144593043:A:T	donor_gain	0.5400

AlphaMissense

0 scored. Top likely-pathogenic:

dbSNP variants (sampled 300 via entrez): RS1157397692 (1:144592919 G>A,T), RS1163135638 (1:144592794 G>T), RS1166096024 (1:144592718 A>G,T), RS1171183906 (1:144592551 T>A,C), RS1171451718 (1:144592650 C>A,T), RS1174199359 (1:144592610 A>T), RS1176854744 (1:144592821 G>A,T), RS1185649839 (1:144592558 CAAAAAAA>C,CA,CAA,CAAAA,CAAAAA,CAAAAAA,CAAAAAAAA,CAAAAAAAAA,CAAAAAAAAAA), RS1186159036 (1:144592558 C>A,T), RS1187281439 (1:144592431 A>C), RS1187466363 (1:144592730 T>C,G), RS1189210512 (1:144592660 G>A), RS1195550100 (1:144592300 G>A), RS1195685603 (1:144592947 T>C), RS1196882220 (1:144592739 C>T)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

1 total (human), top 1 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Caffeine	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.