PPIL4

Summary

PPIL4 (peptidylprolyl isomerase like 4, HGNC:15702) is a protein-coding gene on chromosome 6q25.1, encoding Peptidyl-prolyl cis-trans isomerase-like 4 (Q8WUA2). PPIases accelerate the folding of proteins. It is a common-essential gene (DepMap: required in 94.0% of cancer cell lines).

This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions.

Source: NCBI Gene 85313 — RefSeq curated summary.

At a glance

• GWAS associations: 6
• Clinical variants (ClinVar): 59 total
• Druggable target: yes — 1 molecules with ChEMBL bioactivity
• Cancer dependency (DepMap): dependent in 94.0% of screened cell lines (common-essential)
• MANE Select transcript: NM_139126

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:15702
Approved symbol	PPIL4
Name	peptidylprolyl isomerase like 4
Location	6q25.1
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000131013
Ensembl biotype	protein_coding
OMIM	607609
Entrez	85313

Gene structure

Transcript identifiers

Ensembl transcripts: 3 — 3 protein_coding

ENST00000253329, ENST00000340881, ENST00000876132

RefSeq mRNA: 1 — MANE Select: NM_139126 NM_139126

CCDS: CCDS34550

Canonical transcript exons

ENST00000253329 — 13 exons

Exon	Start	End
ENSE00000439982	149545936	149546043
ENSE00000440004	149504495	149505704
ENSE00000765089	149541519	149541586
ENSE00000765090	149541367	149541431
ENSE00000765091	149540942	149541059
ENSE00000765093	149535596	149535738
ENSE00000765094	149534678	149534774
ENSE00000765095	149533458	149533574
ENSE00000765096	149526652	149526776
ENSE00000813609	149517354	149517450
ENSE00001023248	149521060	149521171
ENSE00001023251	149525143	149525209
ENSE00003555399	149512155	149512302

Expression profiles

Bgee: expression breadth ubiquitous, 257 present calls, max score 98.32.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 22.5551 / max 631.5590, expressed in 1792 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
76129	21.0914	1788
76130	1.4637	976

Top tissues by expression

260 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
calcaneal tendon	UBERON:0003701	98.32	gold quality
tendon	UBERON:0000043	95.35	gold quality
colonic epithelium	UBERON:0000397	92.79	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	92.07	gold quality
left ovary	UBERON:0002119	90.29	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	90.15	gold quality
ventricular zone	UBERON:0003053	89.93	gold quality
adrenal tissue	UBERON:0018303	89.88	gold quality
right ovary	UBERON:0002118	89.64	gold quality
ovary	UBERON:0000992	89.54	gold quality
endometrium	UBERON:0001295	89.52	gold quality
islet of Langerhans	UBERON:0000006	89.24	gold quality
thymus	UBERON:0002370	88.89	gold quality
tendon of biceps brachii	UBERON:0008188	88.71	gold quality
left lobe of thyroid gland	UBERON:0001120	88.61	gold quality
ganglionic eminence	UBERON:0004023	88.57	gold quality
embryo	UBERON:0000922	88.56	gold quality
thyroid gland	UBERON:0002046	88.55	gold quality
medial globus pallidus	UBERON:0002477	88.03	gold quality
adenohypophysis	UBERON:0002196	88.02	gold quality
epithelium of nasopharynx	UBERON:0001951	87.97	gold quality
nasopharynx	UBERON:0001728	87.96	gold quality
omental fat pad	UBERON:0010414	87.90	gold quality
peritoneum	UBERON:0002358	87.89	gold quality
corpus callosum	UBERON:0002336	87.83	gold quality
gall bladder	UBERON:0002110	87.81	gold quality
uterus	UBERON:0000995	87.72	gold quality
seminal vesicle	UBERON:0000998	87.70	gold quality
adipose tissue of abdominal region	UBERON:0007808	87.64	gold quality
descending thoracic aorta	UBERON:0002345	87.63	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	9.64
E-ENAD-17	no	524.59

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

92 targeting PPIL4, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-3924	100.00	72.09	2394
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-4776-3P	100.00	68.73	1340
HSA-MIR-3646	100.00	73.56	5283
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-3662	99.99	73.82	5684
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-6077	99.99	68.04	2299
HSA-MIR-12136	99.98	72.81	5713
HSA-MIR-548N	99.98	71.94	4170
HSA-MIR-568	99.98	69.86	2084
HSA-MIR-520D-5P	99.98	73.34	4883
HSA-MIR-524-5P	99.98	73.43	4882
HSA-MIR-548P	99.98	72.25	3784
HSA-MIR-103A-3P	99.98	69.14	1595
HSA-MIR-107	99.98	69.14	1595
HSA-MIR-3148	99.97	75.06	6478
HSA-MIR-4666A-3P	99.96	71.71	3434
HSA-MIR-128-3P	99.95	71.17	2484
HSA-MIR-216A-3P	99.95	71.19	2505
HSA-MIR-3912-5P	99.95	66.11	925
HSA-MIR-545-3P	99.95	70.74	2783
HSA-MIR-3143	99.93	71.96	3104
HSA-MIR-497-5P	99.92	71.83	2674
HSA-MIR-7-1-3P	99.91	71.53	4384
HSA-MIR-7-2-3P	99.91	71.40	4394
HSA-MIR-589-3P	99.91	69.62	2088
HSA-MIR-15A-5P	99.90	72.80	2787

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 94.0% of screened cell lines, common-essential.

Literature-anchored findings (GeneRIF, showing 2)

• Molecular cloning, structure and expression of a novel nuclear RNA-binding cyclophilin-like gene (PPIL4) from human fetal brain (PMID:11978968)
• PPIL4 is essential for brain angiogenesis and implicated in intracranial aneurysms in humans. (PMID:34887573)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	ppil4	ENSDARG00000043854
mus_musculus	Ppil4	ENSMUSG00000015757
rattus_norvegicus	Ppil4	ENSRNOG00000015552
drosophila_melanogaster	CG5808	FBGN0027617
caenorhabditis_elegans	sig-7	WBGENE00000890

Paralogs (22): PPIE (ENSG00000084072), PPIL2 (ENSG00000100023), PPIF (ENSG00000108179), PPWD1 (ENSG00000113593), NKTR (ENSG00000114857), PPIL1 (ENSG00000137168), PPIG (ENSG00000138398), CWC27 (ENSG00000153015), PPIB (ENSG00000166794), PPIC (ENSG00000168938), PPID (ENSG00000171497), PPIH (ENSG00000171960), PPIL6 (ENSG00000185250), PPIA (ENSG00000196262), PPIAL4G (ENSG00000236334), PPIL3 (ENSG00000240344), PPIAL4A (ENSG00000263353), PPIAL4H (ENSG00000270339), PPIAL4E (ENSG00000271567), PPIAL4F (ENSG00000279782), PPIAL4C (ENSG00000288867), PPIAL4D (ENSG00000289549)

Protein

Protein identifiers

Peptidyl-prolyl cis-trans isomerase-like 4 — Q8WUA2 (reviewed: Q8WUA2)

Alternative names: Cyclophilin-like protein PPIL4, Rotamase PPIL4

All UniProt accessions (2): Q8WUA2, Q5T4S2

UniProt curated annotations — full annotation on UniProt →

Function. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Subcellular location. Nucleus.

Tissue specificity. Abundantly expressed in kidney but has a ubiquitously low expression pattern in other adult tissues.

Similarity. Belongs to the cyclophilin-type PPIase family. PPIL4 subfamily.

RefSeq proteins (1): NP_624311* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000504	RRM_dom	Domain
IPR002130	Cyclophilin-type_PPIase_dom	Domain
IPR012677	Nucleotide-bd_a/b_plait_sf	Homologous_superfamily
IPR029000	Cyclophilin-like_dom_sf	Homologous_superfamily
IPR035538	Cyclophilin_PPIL4	Domain
IPR035542	CRIP	Family
IPR035979	RBD_domain_sf	Homologous_superfamily

Pfam: PF00076, PF00160

Catalyzed reactions (Rhea), 1 shown:

• [protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0) (RHEA:16237)

UniProt features (50 total): strand 19, cross-link 7, helix 5, modified residue 4, turn 4, compositionally biased region 4, region of interest 3, domain 2, chain 1, sequence conflict 1

Structure

Experimental structures (PDB)

7 structures.

PDB	Method	Resolution (Å)
8I0T	ELECTRON MICROSCOPY	3
8I0V	ELECTRON MICROSCOPY	3
7QTT	ELECTRON MICROSCOPY	3.1
8I0U	ELECTRON MICROSCOPY	3.3
9DWV	ELECTRON MICROSCOPY	3.5
8I0S	ELECTRON MICROSCOPY	4.2
8CH6	ELECTRON MICROSCOPY	5.9

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8WUA2-F1	69.56	0.35

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (11): 178, 182, 393, 471, 201, 212, 218, 321, 362, 405, 460

Function

Pathways and Gene Ontology

Reactome pathways

2 pathways

ID	Pathway
R-HSA-72163	mRNA Splicing - Major Pathway
R-HSA-9918481	Dengue Virus-Host Interactions

MSigDB gene sets: 116 (showing top): GSE45365_HEALTHY_VS_MCMV_INFECTION_CD8_TCELL_IFNAR_KO_UP, GSE45365_NK_CELL_VS_CD8A_DC_MCMV_INFECTION_DN, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_DN, GRAESSMANN_RESPONSE_TO_MC_AND_DOXORUBICIN_DN, EFC_Q6, chr6q25, REACTOME_PROCESSING_OF_CAPPED_INTRON_CONTAINING_PRE_MRNA, GOBP_RNA_SPLICING, REACTOME_MRNA_SPLICING, FONTAINE_PAPILLARY_THYROID_CARCINOMA_DN, GRYDER_PAX3FOXO1_ENHANCERS_IN_TADS, REACTOME_METABOLISM_OF_RNA, GOCC_U2_TYPE_SPLICEOSOMAL_COMPLEX, WGGAATGY_TEF1_Q6, GOCC_SPLICEOSOMAL_COMPLEX

GO Biological Process (1): protein peptidyl-prolyl isomerization (GO:0000413)

GO Molecular Function (5): RNA binding (GO:0003723), peptidyl-prolyl cis-trans isomerase activity (GO:0003755), nucleic acid binding (GO:0003676), protein binding (GO:0005515), isomerase activity (GO:0016853)

GO Cellular Component (3): nucleus (GO:0005634), nucleoplasm (GO:0005654), cytosol (GO:0005829)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
mRNA Splicing	1
Dengue Virus Infection	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
binding	2
cellular anatomical structure	2
peptidyl-proline modification	1
nucleic acid binding	1
cis-trans isomerase activity	1
catalytic activity, acting on a protein	1
catalytic activity	1
intracellular membrane-bounded organelle	1
nuclear lumen	1
cytoplasm	1

Protein interactions and networks

STRING

2764 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
PPIL4	FKBP6	O75344	826
PPIL4	FKBP2	P26885	685
PPIL4	TRIP12	Q14669	590
PPIL4	FBXO38	Q6PIJ6	562
PPIL4	USP11	P51784	512
PPIL4	SYF2	O95926	494
PPIL4	PPM1G	O15355	465
PPIL4	SLC7A4	O43246	450
PPIL4	TCEAL4	Q96EI5	447
PPIL4	SLC15A4	Q8N697	429
PPIL4	ZNF622	Q969S3	427
PPIL4	DHX40	Q8IX18	419
PPIL4	PCMT1	P22061	419
PPIL4	SF3A1	Q15459	401
PPIL4	DDX24	Q9GZR7	391

IntAct

74 interactions, top by confidence:

A	B	Type	Score
DHX38	DHX16	psi-mi:“MI:0914”(association)	0.630
PAX6	PPIL4	psi-mi:“MI:0915”(physical association)	0.560
DLG2	PPIL4	psi-mi:“MI:0915”(physical association)	0.560
SNRNP27	UBA6	psi-mi:“MI:0914”(association)	0.530
NAGK	ZBTB43	psi-mi:“MI:0914”(association)	0.530
PES1	AP3B1	psi-mi:“MI:0914”(association)	0.530
ORC6	DCTN6	psi-mi:“MI:0914”(association)	0.530
AMMECR1	HNRNPF	psi-mi:“MI:0914”(association)	0.530
NCBP3	SAP18	psi-mi:“MI:0914”(association)	0.530
DHX40	TRIM27	psi-mi:“MI:0914”(association)	0.530
CHERP	PPIL4	psi-mi:“MI:0915”(physical association)	0.520
NSRP1	PPIL4	psi-mi:“MI:0914”(association)	0.480
H4C16	PPIL4	psi-mi:“MI:0915”(physical association)	0.400
PPIL4	SUOX	psi-mi:“MI:0915”(physical association)	0.400
SDC1	ILVBL	psi-mi:“MI:0915”(physical association)	0.400
SMAD5	PPIL4	psi-mi:“MI:0915”(physical association)	0.370
Junb	RGPD3	psi-mi:“MI:0914”(association)	0.350
XRCC3	DERL1	psi-mi:“MI:0914”(association)	0.350
EMC2	TBL2	psi-mi:“MI:0914”(association)	0.350
MMGT1	DERL1	psi-mi:“MI:0914”(association)	0.350
USP7	STIL	psi-mi:“MI:0914”(association)	0.350
Xpo1	IFT56	psi-mi:“MI:0914”(association)	0.350
LRRK2		psi-mi:“MI:0914”(association)	0.350
TTC29	DHX16	psi-mi:“MI:0914”(association)	0.350
JMJD6	U2SURP	psi-mi:“MI:0914”(association)	0.350
LUC7L2	CASC3	psi-mi:“MI:0914”(association)	0.350
RAB3IL1	PPFIA3	psi-mi:“MI:0914”(association)	0.350
TMA16	SEC24D	psi-mi:“MI:0914”(association)	0.350

BioGRID (200): PPIL4 (Affinity Capture-MS), PPIL4 (Affinity Capture-MS), PPIL4 (Affinity Capture-MS), PPIL4 (Affinity Capture-MS), CCAR2 (Co-fractionation), PPIL4 (Co-fractionation), PPIL4 (Affinity Capture-MS), PPIL4 (Affinity Capture-MS), PPIL4 (Affinity Capture-MS), PPIL4 (Affinity Capture-MS), PPIL4 (Affinity Capture-MS), PPIL4 (Affinity Capture-MS), PPIL4 (Affinity Capture-MS), PPIL4 (Affinity Capture-MS), PPIL4 (Affinity Capture-MS)

ESM2 similar proteins: A3GEV2, A5E032, A8X8D0, C8VBH4, F4K2E9, G5EEW6, O18017, O42941, O74942, O95251, P0C1I6, P0C1I9, P0C1J0, P0C1J2, P0CP88, P0CP89, P25334, P32783, P34498, P52012, P52016, Q02770, Q09637, Q09928, Q17R09, Q19546, Q26548, Q29RZ2, Q2U256, Q4IBK5, Q4IE79, Q4IEV4, Q4P3S3, Q4WAQ9, Q5ARI5, Q5NVL7, Q6C710, Q6CGK4, Q6Q151, Q6Q152

Diamond homologs: D4AY02, G5EEW6, O42941, O74942, O93826, P0C196, P0C1I4, P0C1I5, P0C1I6, P0C1J0, P0C1J1, P0C1J2, P0CP84, P0CP85, P0CP86, P0CP87, P0CP88, P0CP89, P0CP90, P0CP91, P0CP92, P0CP93, P23284, P23285, P24367, P24369, P34790, P52012, P52013, P52014, P52017, P73789, P80311, P87051, Q08E11, Q09637, Q09928, Q13356, Q17QX9, Q27774

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 96 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
mRNA Splicing	9	14.3×	2e-06
Processing of Capped Intron-Containing Pre-mRNA	11	13.1×	1e-07
mRNA Splicing - Major Pathway	16	12.7×	3e-11
mRNA Polyadenylation	7	8.9×	1e-03
Dengue Virus-Host Interactions	11	7.3×	3e-05
Metabolism of RNA	9	5.4×	3e-03

GO biological processes:

GO term	Partners	Fold	FDR
mitotic spindle organization	5	15.6×	5e-03
mRNA splicing, via spliceosome	13	13.7×	6e-09

Disease & clinical

Clinical variants and AI predictions

ClinVar

59 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	47
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

2424 predictions. Top by Δscore:

Variant	Effect	Δscore
6:149505703:TCC:T	acceptor_loss	1.0000
6:149505705:CTGT:C	acceptor_loss	1.0000
6:149517333:A:AC	donor_gain	1.0000
6:149517334:C:CC	donor_gain	1.0000
6:149517355:C:CA	donor_gain	1.0000
6:149525138:CATA:C	donor_loss	1.0000
6:149525140:TACC:T	donor_loss	1.0000
6:149525142:C:CA	donor_loss	1.0000
6:149525205:CACAA:C	acceptor_gain	1.0000
6:149525207:CAA:C	acceptor_gain	1.0000
6:149525208:AA:A	acceptor_gain	1.0000
6:149525210:C:CA	acceptor_loss	1.0000
6:149525210:C:CC	acceptor_gain	1.0000
6:149526646:GATTA:G	donor_loss	1.0000
6:149526647:ATTAC:A	donor_loss	1.0000
6:149526648:TTACC:T	donor_loss	1.0000
6:149526651:CCTT:C	donor_gain	1.0000
6:149526651:CCTTC:C	donor_loss	1.0000
6:149526697:T:C	donor_gain	1.0000
6:149526773:CCAC:C	acceptor_gain	1.0000
6:149526774:CACC:C	acceptor_gain	1.0000
6:149526775:ACCTA:A	acceptor_loss	1.0000
6:149526778:T:A	acceptor_loss	1.0000
6:149533452:T:C	donor_gain	1.0000
6:149533452:TCTTA:T	donor_loss	1.0000
6:149533453:CTTAC:C	donor_loss	1.0000
6:149533454:TTA:T	donor_loss	1.0000
6:149533455:TAC:T	donor_loss	1.0000
6:149533456:A:AC	donor_gain	1.0000
6:149533456:AC:A	donor_gain	1.0000

AlphaMissense

3313 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
6:149521090:A:G	S318P	1.000
6:149521091:C:A	Q317H	1.000
6:149521091:C:G	Q317H	1.000
6:149521094:G:C	S316R	1.000
6:149521094:G:T	S316R	1.000
6:149521096:T:G	S316R	1.000
6:149521097:A:C	F315L	1.000
6:149521097:A:T	F315L	1.000
6:149521098:A:G	F315S	1.000
6:149521099:A:G	F315L	1.000
6:149521104:A:T	V313E	1.000
6:149521108:G:C	H312D	1.000
6:149521110:A:C	I311R	1.000
6:149521110:A:G	I311T	1.000
6:149521110:A:T	I311K	1.000
6:149521112:T:A	R310S	1.000
6:149521112:T:G	R310S	1.000
6:149521113:C:G	R310T	1.000
6:149521121:A:C	D307E	1.000
6:149521121:A:T	D307E	1.000
6:149521122:T:A	D307V	1.000
6:149521123:C:G	D307H	1.000
6:149521125:A:T	I306K	1.000
6:149521128:A:G	L305P	1.000
6:149521128:A:T	L305H	1.000
6:149521139:C:A	M301I	1.000
6:149521139:C:G	M301I	1.000
6:149521139:C:T	M301I	1.000
6:149521140:A:G	M301T	1.000
6:149521160:A:C	C294W	1.000

dbSNP variants (sampled 300 via entrez): RS1000146145 (6:149535993 T>C), RS1000207476 (6:149547863 T>G), RS1000255004 (6:149511457 C>T), RS1000287239 (6:149529533 C>A,T), RS1000314723 (6:149531328 G>A), RS1000446045 (6:149537719 GAAT>G), RS1000504895 (6:149530721 A>G), RS1000531095 (6:149543102 T>C), RS1000565201 (6:149523713 A>C), RS1000676604 (6:149524012 T>A,C), RS1000800046 (6:149506061 C>G,T), RS1000860672 (6:149538065 G>A), RS1000939108 (6:149510178 A>G), RS1000990214 (6:149519403 A>C,G), RS1001102337 (6:149536566 A>C)

Disease associations

OMIM: gene MIM:607609 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

6 associations (top):

Study	Trait	p-value
GCST002318_75	Rheumatoid arthritis	3.000000e-09
GCST002318_89	Rheumatoid arthritis	5.000000e-08
GCST006959_118	Rheumatoid arthritis	7.000000e-07
GCST006959_97	Rheumatoid arthritis	5.000000e-06
GCST010702_29	Subcortical volume (MOSTest)	7.000000e-11
GCST010703_317	Brain morphology (MOSTest)	7.000000e-22

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0004346	neuroimaging measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL5725153 (SINGLE PROTEIN)

Molecules with ChEMBL bioactivity

1 molecules (phase ≥1), by development phase (incl. off-target/promiscuous compounds). Patent mentions across the top 20 by phase: 1,538 (via chembl_molecule»patent_compound — counts attach to the compound, not the gene–compound relationship, so off-target/promiscuous molecules can dominate).

Molecule	Name	Phase	Patents
CHEMBL1232461	MOLIBRESIB	2	1,538

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

1 potent at pChembl≥5 of 1 total, top 1 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.12	IC50	7610	nM	MOLIBRESIB

PubChem BioAssay actives

1 with measured affinity, of 6 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
2-[(4S)-6-(4-chlorophenyl)-8-methoxy-1-methyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepin-4-yl]-N-ethylacetamide	2179043: Inhibition of PPIL4 (unknown origin) incubated for 1 hr by colloidal coomassie staining based LC-MS/MS analysis	ic50	7.6100	uM

CTD chemical–gene interactions

42 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	affects methylation, increases abundance, increases expression	3
Valproic Acid	decreases expression, affects cotreatment, increases expression	3
Quercetin	affects expression, decreases expression	2
aristolochic acid I	decreases expression, increases expression	1
GSK-J4	increases expression	1
FR900359	increases phosphorylation	1
dicrotophos	decreases expression	1
mono-(2-ethylhexyl)phthalate	decreases expression	1
tris(1,3-dichloro-2-propyl)phosphate	increases expression	1
zinc chromate	increases abundance, increases expression	1
manganese chloride	increases abundance, increases expression	1
coumarin	increases phosphorylation	1
di-n-butylphosphoric acid	affects expression	1
chromium hexavalent ion	increases abundance, increases expression	1
perfluorooctane sulfonic acid	decreases expression	1
K 7174	increases expression	1
abrine	increases expression	1
Grape Seed Proanthocyanidins	affects cotreatment, increases expression	1
jinfukang	affects cotreatment, decreases expression	1
Acetaminophen	decreases expression	1
Arsenic	increases abundance, increases expression	1
Cadmium	increases expression	1
Caffeine	affects phosphorylation	1
Catechin	affects cotreatment, increases expression	1
Cisplatin	affects cotreatment, decreases expression	1
Enzyme Inhibitors	decreases activity, increases O-linked glycosylation	1
Formaldehyde	increases expression	1
Hydralazine	affects cotreatment, increases expression	1
Manganese	increases abundance, increases expression	1
Methotrexate	increases expression	1

ChEMBL screening assays

6 unique, capped per target: 6 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5697773	Binding	Inhibition of PPIL4 (unknown origin) assessed as fold change at 10 uM incubated for 1 hr by colloidal coomassie staining based LC-MS/MS analysis	Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia. — Nature

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.