PPP1R2B

Summary

PPP1R2B (PPP1R2 family member B, HGNC:16318) is a protein-coding gene on chromosome 5q33.3, encoding Protein phosphatase inhibitor 2 family member B (Q6NXS1). Inhibitor of protein-phosphatase 1.

Predicted to enable protein phosphatase inhibitor activity. Predicted to be involved in intracellular signal transduction.

Source: NCBI Gene 153743 — RefSeq curated summary.

At a glance

• MANE Select transcript: NM_206858

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:16318
Approved symbol	PPP1R2B
Name	PPP1R2 family member B
Location	5q33.3
Locus type	gene with protein product
Status	Approved
Aliases	MGC87149
Ensembl gene	ENSG00000231989
Ensembl biotype	protein_coding
Entrez	153743

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000522232

RefSeq mRNA: 1 — MANE Select: NM_206858 NM_206858

CCDS: CCDS87340

Canonical transcript exons

ENST00000522232 — 1 exons

Exon	Start	End
ENSE00002103948	156850295	156852528

Expression profiles

Bgee: expression breadth broad, 11 present calls, max score 84.33.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.4312 / max 69.4671, expressed in 148 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
59782	0.3551	147
59785	0.0354	3
59783	0.0353	3
59784	0.0054	3

Top tissues by expression

123 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	84.33	gold quality
testis	UBERON:0000473	84.19	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	84.02	gold quality
right testis	UBERON:0004534	83.29	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	62.17	silver quality
hindlimb stylopod muscle	UBERON:0004252	45.60	gold quality
bone marrow cell	CL:0002092	44.35	gold quality
colonic epithelium	UBERON:0000397	41.98	gold quality
skeletal muscle tissue	UBERON:0001134	40.70	gold quality
muscle tissue	UBERON:0002385	40.35	gold quality
leukocyte	CL:0000738	40.08	gold quality
sural nerve	UBERON:0015488	39.67	gold quality
bone marrow	UBERON:0002371	39.28	gold quality
monocyte	CL:0000576	39.27	gold quality
ganglionic eminence	UBERON:0004023	37.30	gold quality
granulocyte	CL:0000094	36.92	gold quality
blood	UBERON:0000178	36.55	gold quality
ventricular zone	UBERON:0003053	36.48	gold quality
cortical plate	UBERON:0005343	36.47	gold quality
superior frontal gyrus	UBERON:0002661	35.52	gold quality
prefrontal cortex	UBERON:0000451	35.32	silver quality
lymph node	UBERON:0000029	34.73	gold quality
muscle of leg	UBERON:0001383	33.93	gold quality
tonsil	UBERON:0002372	33.64	gold quality
smooth muscle tissue	UBERON:0001135	33.32	gold quality
gastrocnemius	UBERON:0001388	33.25	gold quality
frontal cortex	UBERON:0001870	31.88	silver quality
right lung	UBERON:0002167	31.76	gold quality
Brodmann (1909) area 9	UBERON:0013540	31.28	gold quality
liver	UBERON:0002107	31.04	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	4.73

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 1)

• PPP1R2P3 protein binds directly to PPP1CC and that this inhibitor cannot be phosphorylated by GSK3. (PMID:23506001)

Cross-species orthologs

6 orthologs

Organism	Symbol	Gene ID
danio_rerio	ppp1r2	ENSDARG00000054007
mus_musculus	Ppp1r2	ENSMUSG00000047714
mus_musculus	Ppp1r2-ps1	ENSMUSG00000094843
mus_musculus	Ppp1r2-ps6	ENSMUSG00000095869
mus_musculus	Ppp1r2-ps5	ENSMUSG00000121976
rattus_norvegicus	Ppp1r2	ENSRNOG00000001733

Paralogs (2): PPP1R2C (ENSG00000102055), PPP1R2 (ENSG00000184203)

Protein

Protein identifiers

Protein phosphatase inhibitor 2 family member B — Q6NXS1 (reviewed: Q6NXS1)

Alternative names: PPP1R2 family member B, Protein phosphatase 1, regulatory subunit 2 pseudogene 3, Protein phosphatase inhibitor 2-like protein 3

All UniProt accessions (1): Q6NXS1

UniProt curated annotations — full annotation on UniProt →

Function. Inhibitor of protein-phosphatase 1.

Subunit / interactions. Interacts with PPP1CC.

Tissue specificity. Only detected in spermatozoa, both heads and tails.

Similarity. Belongs to the protein phosphatase inhibitor 2 family.

RefSeq proteins (1): NP_996740* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR007062	PPI-2	Family

Pfam: PF04979

UniProt features (23 total): modified residue 8, compositionally biased region 6, region of interest 6, initiator methionine 1, chain 1, sequence conflict 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q6NXS1-F1	68.64	0.19

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (8): 2, 44, 89, 92, 121, 122, 127, 130

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 20 (showing top): HNF1_Q6, GOBP_GENERATION_OF_PRECURSOR_METABOLITES_AND_ENERGY, GOBP_CARBOHYDRATE_METABOLIC_PROCESS, HNF1_01, CART1_01, AR_Q2, GOMF_PHOSPHATASE_INHIBITOR_ACTIVITY, GOMF_ENZYME_INHIBITOR_ACTIVITY, GOBP_ENERGY_RESERVE_METABOLIC_PROCESS, GOMF_ENZYME_REGULATOR_ACTIVITY, GOMF_PHOSPHATASE_REGULATOR_ACTIVITY, S8_01, GOBP_POLYSACCHARIDE_METABOLIC_PROCESS, CTTTAAR_UNKNOWN, chr5q33

GO Biological Process (3): glycogen metabolic process (GO:0005977), regulation of signal transduction (GO:0009966), intracellular signal transduction (GO:0035556)

GO Molecular Function (2): protein phosphatase inhibitor activity (GO:0004864), protein binding (GO:0005515)

GO Cellular Component (0):

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
signal transduction	2
energy reserve metabolic process	1
glucan metabolic process	1
regulation of cell communication	1
regulation of signaling	1
regulation of response to stimulus	1
intracellular anatomical structure	1
phosphoprotein phosphatase activity	1
phosphatase inhibitor activity	1
protein phosphatase regulator activity	1
binding	1

Protein interactions and networks

STRING

118 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
PPP1R2B	PPP1R11	O60927	554
PPP1R2B	GARIN3	Q8TC56	507
PPP1R2B	PPP1R7	Q15435	446
PPP1R2B	TIMD4	Q96H15	352
PPP1R2B	SGCD	Q92629	326
PPP1R2B	APOO	Q9BUR5	311
PPP1R2B	MED7	O43513	310
PPP1R2B	PPP1R8	Q12972	303
PPP1R2B	PHC1	P78364	266
PPP1R2B	CERT1	Q9Y5P4	224
PPP1R2B	SPAG9	O60271	222
PPP1R2B	UGDH	O60701	220
PPP1R2B	AK7	Q96M32	212
PPP1R2B	FSIP2	Q5CZC0	211
PPP1R2B	HLA-DMB	P28068	204

IntAct

26 interactions, top by confidence:

A	B	Type	Score
PPP1CB	PPP1R2B	psi-mi:“MI:0915”(physical association)	0.720
PPP1CC	PPP1R2B	psi-mi:“MI:0915”(physical association)	0.670
PPP1R2B	PPP1CC	psi-mi:“MI:0915”(physical association)	0.670
PPP1CA	PPP1R2B	psi-mi:“MI:0915”(physical association)	0.560
H2BW1	PPP1R2B	psi-mi:“MI:0915”(physical association)	0.560
MAPT	SHTN1	psi-mi:“MI:0914”(association)	0.350
MAPT	PITPNM1	psi-mi:“MI:2364”(proximity)	0.270
MAPT	DCTN6	psi-mi:“MI:2364”(proximity)	0.270
PPP1CC	PPP1R2B	psi-mi:“MI:0915”(physical association)	0.000
PPP1CB	PPP1R2B	psi-mi:“MI:0915”(physical association)	0.000
H2BW1	PPP1R2B	psi-mi:“MI:0915”(physical association)	0.000

ESM2 similar proteins: A2VDK6, B5X3I1, O08585, O43290, O88271, P04973, P04975, P08081, P08082, P09496, P09497, P09741, P13805, P41236, P50751, P50753, Q02614, Q0VFP5, Q2QY04, Q3U155, Q40554, Q5BK07, Q5EA95, Q5I0B5, Q5PQS7, Q5RGJ6, Q5XIW8, Q5ZIH9, Q5ZK95, Q6INR1, Q6IRU5, Q6NUB2, Q6NXS1, Q6PII3, Q75QI0, Q75UQ2, Q7SXU0, Q8BH43, Q8LDQ4, Q8R1N0

Diamond homologs: O14990, P11845, P41236, P50411, Q3SZX2, Q4R615, Q6NXS1, Q96PQ5, Q9DCL8

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

0 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	0
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

0 predictions. Top by Δscore:

AlphaMissense

1366 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
5:156850980:T:C	F140L	0.892
5:156850982:T:A	F140L	0.892
5:156850982:T:G	F140L	0.892
5:156850603:T:C	I14T	0.859
5:156850994:G:C	R144S	0.851
5:156850994:G:T	R144S	0.851
5:156851032:C:A	A157D	0.843
5:156850610:G:C	K16N	0.819
5:156850610:G:T	K16N	0.819
5:156850598:G:C	K12N	0.817
5:156850598:G:T	K12N	0.817
5:156851041:T:C	L160S	0.797
5:156851036:A:C	R158S	0.787
5:156851036:A:T	R158S	0.787
5:156850599:G:A	G13R	0.771
5:156850599:G:C	G13R	0.771
5:156850975:G:C	R138P	0.768
5:156851044:T:C	I161T	0.764
5:156851031:G:C	A157P	0.757
5:156850701:T:A	W47R	0.737
5:156850701:T:C	W47R	0.737
5:156851029:T:C	L156P	0.736
5:156850606:T:C	L15S	0.733
5:156850603:T:G	I14S	0.724
5:156851023:T:C	I154T	0.714
5:156850993:G:C	R144T	0.710
5:156851012:A:C	E150D	0.704
5:156851012:A:T	E150D	0.704
5:156850609:A:C	K16T	0.691
5:156850599:G:T	G13W	0.680

dbSNP variants (sampled 300 via entrez): RS1000427814 (5:156848706 C>G,T), RS1000798801 (5:156848471 C>T), RS1001680662 (5:156851742 CT>C), RS1002354084 (5:156851904 T>G), RS1003122862 (5:156848950 A>G), RS1003153764 (5:156849292 A>G), RS1003619580 (5:156852913 G>T), RS1004456995 (5:156852831 A>G), RS10045208 (5:156853003 A>C,T), RS10054293 (5:156852582 G>A), RS1006284659 (5:156849808 T>C), RS10068033 (5:156852012 C>T), RS1006901078 (5:156851409 C>G,T), RS1006908121 (5:156848376 C>A,T), RS1007868809 (5:156852225 G>A)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

3 total (human), top 3 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Polychlorinated Biphenyls	affects expression	1
Rotenone	decreases expression	1
Valproic Acid	decreases methylation	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.