PPP4R3B

Summary

PPP4R3B (protein phosphatase 4 regulatory subunit 3B, HGNC:29267) is a protein-coding gene on chromosome 2p16.1, encoding Serine/threonine-protein phosphatase 4 regulatory subunit 3B (Q5MIZ7). Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4).

Predicted to enable protein phosphatase activator activity. Involved in regulation of double-strand break repair. Located in centrosome; chromatin; and nuclear speck. Part of protein phosphatase 4 complex.

Source: NCBI Gene 57223 — RefSeq curated summary.

At a glance

• GWAS associations: 3
• Clinical variants (ClinVar): 119 total
• Druggable target: yes
• MANE Select transcript: NM_001122964

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:29267
Approved symbol	PPP4R3B
Name	protein phosphatase 4 regulatory subunit 3B
Location	2p16.1
Locus type	gene with protein product
Status	Approved
Aliases	PSY2, FLFL2, KIAA1387, FLJ31474
Ensembl gene	ENSG00000275052
Ensembl biotype	protein_coding
OMIM	610352
Entrez	57223

Gene structure

Transcript identifiers

Ensembl transcripts: 57 — 54 protein_coding, 2 protein_coding_CDS_not_defined, 1 retained_intron

ENST00000470801, ENST00000482826, ENST00000611717, ENST00000612688, ENST00000616288, ENST00000616407, ENST00000890050, ENST00000890052, ENST00000890053, ENST00000890054, ENST00000890055, ENST00000890056, ENST00000890057, ENST00000890058, ENST00000890059, ENST00000890060, ENST00000890061, ENST00000890062, ENST00000890063, ENST00000890064, ENST00000890065, ENST00000890066, ENST00000890067, ENST00000890068, ENST00000890069, ENST00000890070, ENST00000890071, ENST00000890072, ENST00000890073, ENST00000890074, ENST00000890075, ENST00000890076, ENST00000890077, ENST00000890078, ENST00000890079, ENST00000890080, ENST00000890081, ENST00000890082, ENST00000890083, ENST00000890084, ENST00000890085, ENST00000890086, ENST00000925128, ENST00000925129, ENST00000925130, ENST00000925131, ENST00000925132, ENST00000925133, ENST00000953461, ENST00000953462, ENST00000953463, ENST00000953464, ENST00000953465, ENST00000953466, ENST00000953467, ENST00000953468, ENST00000953469

RefSeq mRNA: 3 — MANE Select: NM_001122964 NM_001122964, NM_001282850, NM_020463

CCDS: CCDS1855, CCDS46289, CCDS62913

Canonical transcript exons

ENST00000616407 — 17 exons

Exon	Start	End
ENSE00003716706	55558775	55558968
ENSE00003719374	55581567	55581698
ENSE00003727370	55578247	55578342
ENSE00003728592	55547292	55550006
ENSE00003729471	55585051	55585167
ENSE00003733248	55598416	55599039
ENSE00003733493	55615451	55615506
ENSE00003735548	55573619	55573777
ENSE00003738312	55568194	55568363
ENSE00003741049	55579679	55579781
ENSE00003743674	55617144	55617622
ENSE00003746152	55577315	55577356
ENSE00003746425	55586618	55586734
ENSE00003749064	55564313	55564497
ENSE00003750114	55564902	55565041
ENSE00003750954	55588879	55588956
ENSE00003752712	55603978	55604076

Expression profiles

Bgee: expression breadth ubiquitous, 276 present calls, max score 97.52.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 32.0002 / max 516.7301, expressed in 1809 samples.

FANTOM5 promoters (7 alternative TSS)

Promoter ID	TPM avg	Samples expressed
28474	27.8202	1799
28480	1.6025	840
28478	1.2670	784
28477	0.6825	400
28479	0.5653	296
28473	0.0583	26
28472	0.0044	3

Top tissues by expression

287 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
bronchial epithelial cell	CL:0002328	97.52	gold quality
buccal mucosa cell	CL:0002336	97.47	gold quality
jejunal mucosa	UBERON:0000399	96.48	gold quality
ventricular zone	UBERON:0003053	95.87	gold quality
calcaneal tendon	UBERON:0003701	95.73	gold quality
ganglionic eminence	UBERON:0004023	95.70	gold quality
cortical plate	UBERON:0005343	95.32	gold quality
mucosa of paranasal sinus	UBERON:0005030	94.80	gold quality
mucosa of sigmoid colon	UBERON:0004993	94.67	gold quality
colonic epithelium	UBERON:0000397	94.26	gold quality
tonsil	UBERON:0002372	94.25	gold quality
oral cavity	UBERON:0000167	94.05	gold quality
colonic mucosa	UBERON:0000317	94.04	gold quality
penis	UBERON:0000989	93.60	gold quality
caput epididymis	UBERON:0004358	93.59	gold quality
upper leg skin	UBERON:0004262	93.57	gold quality
epithelium of nasopharynx	UBERON:0001951	93.55	gold quality
superficial temporal artery	UBERON:0001614	93.49	gold quality
jejunum	UBERON:0002115	93.40	gold quality
rectum	UBERON:0001052	93.36	gold quality
monocyte	CL:0000576	93.27	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	92.96	gold quality
endothelial cell	CL:0000115	92.94	gold quality
leukocyte	CL:0000738	92.92	gold quality
mononuclear cell	CL:0000842	92.88	gold quality
skin of hip	UBERON:0001554	92.69	gold quality
endometrium	UBERON:0001295	92.68	gold quality
gall bladder	UBERON:0002110	92.64	gold quality
adrenal tissue	UBERON:0018303	92.62	gold quality
islet of Langerhans	UBERON:0000006	92.60	gold quality

Single-cell (SCXA)

Detected in 4 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-GEOD-124858	no	221.49
E-CURD-112	no	2.27
E-HCAD-31	no	1.98
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

154 targeting PPP4R3B, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-LET-7A-3P	100.00	74.03	3932
HSA-LET-7B-3P	100.00	74.08	3913
HSA-LET-7F-1-3P	100.00	74.02	3928
HSA-MIR-98-3P	100.00	74.08	3907
HSA-MIR-518D-5P	100.00	67.51	979
HSA-MIR-518E-5P	100.00	67.66	954
HSA-MIR-518F-5P	100.00	67.51	979
HSA-MIR-519A-5P	100.00	67.66	954
HSA-MIR-519B-5P	100.00	67.66	954
HSA-MIR-519C-5P	100.00	67.66	954
HSA-MIR-520C-5P	100.00	67.51	979
HSA-MIR-522-5P	100.00	67.66	954
HSA-MIR-523-5P	100.00	67.66	954
HSA-MIR-526A-5P	100.00	67.51	979
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-371B-5P	99.99	75.34	4759
HSA-MIR-499A-5P	99.98	70.79	1323
HSA-MIR-8068	99.98	73.85	2376
HSA-MIR-103A-3P	99.98	69.14	1595
HSA-MIR-107	99.98	69.14	1595
HSA-MIR-548N	99.98	71.94	4170
HSA-MIR-373-5P	99.98	75.36	4753
HSA-MIR-616-5P	99.98	75.58	4775
HSA-MIR-3688-3P	99.97	72.02	2834
HSA-MIR-3148	99.97	75.06	6478
HSA-MIR-5688	99.96	73.23	4504
HSA-MIR-495-3P	99.96	72.81	4197
HSA-MIR-3658	99.96	73.87	4379
HSA-MIR-4666A-3P	99.96	71.71	3434

Literature-anchored findings (GeneRIF, showing 1)

• A three-protein PP4 phosphatase complex in mammalian cells, containing PP4C, PP4R2, and PP4R3beta, specifically dephosphorylates ATR-mediated gamma-H2AX generated during DNA replication. (PMID:18614045)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	ppp4r3b	ENSDARG00000103415
mus_musculus	Ppp4r3b	ENSMUSG00000020463
rattus_norvegicus	Ppp4r3b	ENSRNOG00000003712
drosophila_melanogaster	flfl	FBGN0024555
caenorhabditis_elegans		WBGENE00018285

Paralogs (2): PPP4R3A (ENSG00000100796), PPP4R3C (ENSG00000224960)

Protein

Protein identifiers

Serine/threonine-protein phosphatase 4 regulatory subunit 3B — Q5MIZ7 (reviewed: Q5MIZ7)

Alternative names: SMEK homolog 2

All UniProt accessions (1): Q5MIZ7

UniProt curated annotations — full annotation on UniProt →

Function. Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers.

Subunit / interactions. Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complex PPP4C-PPP4R2-PPP4R3B.

Subcellular location. Cytoplasm. Cytoskeleton. Microtubule organizing center. Centrosome. Nucleus.

Tissue specificity. Moderately expressed in tissues and specific brain regions examined.

Miscellaneous. May be due to intron retention.

Similarity. Belongs to the SMEK family.

Isoforms (5)

UniProt ID	Names	Canonical?
Q5MIZ7-1	1	yes
Q5MIZ7-2	2
Q5MIZ7-3	3
Q5MIZ7-4	4
Q5MIZ7-5	5

RefSeq proteins (3): NP_001116436, NP_001269779, NP_065196 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR006887	P4R3-like_central_dom	Domain
IPR011993	PH-like_dom_sf	Homologous_superfamily
IPR016024	ARM-type_fold	Homologous_superfamily
IPR051137	PP4R3-like	Family
IPR055236	EVH1_PP4R3	Domain

Pfam: PF04802, PF22972

UniProt features (19 total): splice variant 6, compositionally biased region 4, modified residue 3, sequence variant 2, chain 1, domain 1, sequence conflict 1, region of interest 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q5MIZ7-F1	76.48	0.59

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (3): 117, 695, 840

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 268 (showing top): GSE45365_HEALTHY_VS_MCMV_INFECTION_CD8_TCELL_IFNAR_KO_UP, GOBP_REGULATION_OF_DOUBLE_STRAND_BREAK_REPAIR, GGGACCA_MIR133A_MIR133B, TGGTGCT_MIR29A_MIR29B_MIR29C, MODULE_97, RODRIGUES_THYROID_CARCINOMA_ANAPLASTIC_UP, RODRIGUES_THYROID_CARCINOMA_POORLY_DIFFERENTIATED_UP, GCANCTGNY_MYOD_Q6, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_DN, IVANOVA_HEMATOPOIESIS_LATE_PROGENITOR, MODULE_182, GOBP_REGULATION_OF_DNA_REPAIR, YY1_Q6, GOBP_POSITIVE_REGULATION_OF_CARBOHYDRATE_METABOLIC_PROCESS, GOCC_MICROTUBULE_ORGANIZING_CENTER

GO Biological Process (4): gluconeogenesis (GO:0006094), DNA damage response (GO:0006974), positive regulation of gluconeogenesis (GO:0045722), regulation of double-strand break repair (GO:2000779)

GO Molecular Function (2): protein phosphatase activator activity (GO:0072542), protein phosphatase regulator activity (GO:0019888)

GO Cellular Component (8): chromatin (GO:0000785), nucleoplasm (GO:0005654), cytoplasm (GO:0005737), centrosome (GO:0005813), nuclear speck (GO:0016607), protein phosphatase 4 complex (GO:0030289), nucleus (GO:0005634), cytoskeleton (GO:0005856)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
phosphoprotein phosphatase activity	2
glucose metabolic process	1
hexose biosynthetic process	1
cellular response to stress	1
gluconeogenesis	1
regulation of gluconeogenesis	1
positive regulation of biosynthetic process	1
positive regulation of glucose metabolic process	1
regulation of DNA repair	1
double-strand break repair	1
phosphatase activator activity	1
protein phosphatase regulator activity	1
phosphatase regulator activity	1
protein phosphatase binding	1
chromosome	1
nuclear lumen	1
intracellular anatomical structure	1
centriole	1
microtubule organizing center	1
nuclear ribonucleoprotein granule	1
protein serine/threonine phosphatase complex	1
intracellular membrane-bounded organelle	1
intracellular membraneless organelle	1

Protein interactions and networks

STRING

1524 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
PPP4R3B	PPP4C	P33172	995
PPP4R3B	PPP4R2	Q9NY27	954
PPP4R3B	RANBP1	P43487	745
PPP4R3B	ATR	Q13535	704
PPP4R3B	VASP	P50552	669
PPP4R3B	MAP2K1	Q02750	615
PPP4R3B	PPP4R1	Q8TF05	568
PPP4R3B	PLEK2	Q9NYT0	540
PPP4R3B	CHEK2	O96017	537
PPP4R3B	PPP4R4	Q6NUP7	533
PPP4R3B	PLEK	P08567	493
PPP4R3B	BSDC1	Q9NW68	491
PPP4R3B	MRPL51	Q4U2R6	490
PPP4R3B	CENPC	Q03188	488
PPP4R3B	DCP2	Q8IU60	485

IntAct

57 interactions, top by confidence:

A	B	Type	Score
BNIP3	BNIP3L	psi-mi:“MI:0914”(association)	0.970
PPP4R2	PPP4C	psi-mi:“MI:0914”(association)	0.950
PPP4R3B	PPP4C	psi-mi:“MI:0914”(association)	0.890
PPP4R2	TIPRL	psi-mi:“MI:0914”(association)	0.800
PPP4C	TCP1	psi-mi:“MI:0914”(association)	0.730
DHX38	PPP4C	psi-mi:“MI:0914”(association)	0.730
PPP4C	SUPT5H	psi-mi:“MI:0914”(association)	0.640
NDUFA13	NDUFS8	psi-mi:“MI:0914”(association)	0.640
DHX38	DHX16	psi-mi:“MI:0914”(association)	0.630
PPP4R2	SF3B1	psi-mi:“MI:0914”(association)	0.570
PPP4R2	SF3B1	psi-mi:“MI:2364”(proximity)	0.570
COMTD1	IFRD1	psi-mi:“MI:0914”(association)	0.530
CCDC6	LZTS3	psi-mi:“MI:0914”(association)	0.530
PMPCB	AMY1A	psi-mi:“MI:0914”(association)	0.530
CRAMP1	NPAT	psi-mi:“MI:0914”(association)	0.530
CUEDC1	TOM1	psi-mi:“MI:0914”(association)	0.530
TSSC4	SNRNP200	psi-mi:“MI:0914”(association)	0.530
SPG11	PPP4R3B	psi-mi:“MI:0915”(physical association)	0.370
PPP4R3B		psi-mi:“MI:0915”(physical association)	0.370
RNGTT	SUPT5H	psi-mi:“MI:0914”(association)	0.350
SYK	PLEKHD1	psi-mi:“MI:0914”(association)	0.350
GPR17	TMEM120B	psi-mi:“MI:0914”(association)	0.350
TAFA3	FUOM	psi-mi:“MI:0914”(association)	0.350
EFNB1	KRBA1	psi-mi:“MI:0914”(association)	0.350
CT45A6	AP3B1	psi-mi:“MI:0914”(association)	0.350
ZNF785	CASK	psi-mi:“MI:0914”(association)	0.350
PPP4C	MID1	psi-mi:“MI:0914”(association)	0.350

BioGRID (115): SMEK2 (Affinity Capture-MS), SMEK2 (Affinity Capture-MS), SMEK2 (Affinity Capture-MS), SMEK2 (Affinity Capture-MS), HNRNPU (Co-fractionation), OSBPL8 (Co-fractionation), PPP4C (Co-fractionation), SMEK2 (Co-fractionation), SMEK2 (Co-fractionation), SMEK2 (Affinity Capture-MS), SMEK2 (Affinity Capture-MS), SMEK2 (Affinity Capture-MS), SMEK2 (Affinity Capture-MS), SMEK2 (Affinity Capture-MS), SMEK2 (Affinity Capture-MS)

ESM2 similar proteins: A0A1D5P556, A0A3Q1LSX9, A2A5R2, A2APV2, B0DOB5, B2RQE8, D3ZYR1, D4A631, F1LVW7, F1M775, F4IUX6, G3X9K3, O08808, O46382, O60308, O60610, O75674, O95466, Q07139, Q0IHV1, Q0JRZ9, Q3UQN2, Q4S6U8, Q5MIZ7, Q5R807, Q5SP90, Q6DFT3, Q6IN85, Q6INN7, Q6NTV6, Q6NXC0, Q6P2K6, Q6ZPF4, Q7TSU1, Q7ZX60, Q801Q7, Q80U19, Q86T65, Q8BPM0, Q8IVF7

Diamond homologs: Q3V0Y1, Q4S6U8, Q54I18, Q5MIZ7, Q5SP90, Q6DFT3, Q6IN85, Q6INN7, Q6P2K6, Q6ZMV5, Q7ZX60, Q801Q7, Q922R5, Q9VFS5, Q9Y7J8, Q6CQ91, Q75E32

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 78 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
mRNA Splicing - Major Pathway	7	8.9×	3e-03

GO biological processes:

GO term	Partners	Fold	FDR
mRNA splicing, via spliceosome	8	10.5×	4e-04

Disease & clinical

Clinical variants and AI predictions

ClinVar

119 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	89
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

3222 predictions. Top by Δscore:

Variant	Effect	Δscore
2:55558770:CCTA:C	donor_loss	1.0000
2:55558771:CTAC:C	donor_loss	1.0000
2:55558774:C:CG	donor_loss	1.0000
2:55558966:TTG:T	acceptor_gain	1.0000
2:55558969:C:CC	acceptor_gain	1.0000
2:55564307:TAATA:T	donor_loss	1.0000
2:55564309:ATAC:A	donor_loss	1.0000
2:55564310:TACCT:T	donor_loss	1.0000
2:55564311:A:AT	donor_loss	1.0000
2:55564312:C:CT	donor_loss	1.0000
2:55564345:T:C	donor_gain	1.0000
2:55564493:GTACA:G	acceptor_gain	1.0000
2:55564494:TACA:T	acceptor_gain	1.0000
2:55564496:CA:C	acceptor_gain	1.0000
2:55564496:CACTG:C	acceptor_loss	1.0000
2:55564498:C:CC	acceptor_gain	1.0000
2:55564498:C:CG	acceptor_loss	1.0000
2:55564499:T:G	acceptor_loss	1.0000
2:55564894:A:C	donor_gain	1.0000
2:55564896:CAATA:C	donor_loss	1.0000
2:55564898:ATAC:A	donor_loss	1.0000
2:55564899:TACCT:T	donor_loss	1.0000
2:55564900:A:C	donor_loss	1.0000
2:55564901:C:A	donor_loss	1.0000
2:55565038:CTTC:C	acceptor_gain	1.0000
2:55565039:TTC:T	acceptor_gain	1.0000
2:55565041:CCTG:C	acceptor_loss	1.0000
2:55565042:C:A	acceptor_loss	1.0000
2:55565042:C:CC	acceptor_gain	1.0000
2:55565043:T:G	acceptor_loss	1.0000

AlphaMissense

5646 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
2:55568201:A:T	I643K	1.000
2:55568225:G:T	A635D	1.000
2:55568226:C:G	A635P	1.000
2:55568229:A:G	S634P	1.000
2:55568237:A:G	L631P	1.000
2:55568239:A:C	N630K	1.000
2:55568239:A:T	N630K	1.000
2:55568264:A:G	L622P	1.000
2:55568276:A:T	V618D	1.000
2:55568288:A:G	L614P	1.000
2:55568309:C:G	R607P	1.000
2:55568316:A:C	Y605D	1.000
2:55568327:T:A	K601I	1.000
2:55568330:A:G	L600P	1.000
2:55568344:C:A	R595S	1.000
2:55568344:C:G	R595S	1.000
2:55568345:C:A	R595M	1.000
2:55568345:C:G	R595T	1.000
2:55568354:C:G	R592P	1.000
2:55568355:G:T	R592S	1.000
2:55568357:A:G	L591P	1.000
2:55568360:G:T	A590D	1.000
2:55573621:A:C	L588W	1.000
2:55573629:A:C	F585L	1.000
2:55573629:A:T	F585L	1.000
2:55573631:A:G	F585L	1.000
2:55573657:A:C	L576W	1.000
2:55573695:T:A	K563N	1.000
2:55573695:T:G	K563N	1.000
2:55573696:T:A	K563I	1.000

dbSNP variants (sampled 300 via entrez): RS1000011209 (2:55564322 T>C), RS1000021861 (2:55581949 A>G), RS1000042525 (2:55616229 G>A,C), RS1000105937 (2:55570224 T>C), RS1000119507 (2:55601539 C>G), RS1000128216 (2:55576095 C>T), RS1000159911 (2:55593948 G>A,T), RS1000186382 (2:55599921 G>A), RS1000212299 (2:55570771 T>C), RS1000214475 (2:55611669 T>C,G), RS1000218652 (2:55561894 G>T), RS1000247003 (2:55611840 C>T), RS1000270155 (2:55596523 G>A), RS1000320031 (2:55578305 G>A), RS1000342442 (2:55572686 T>C)

Disease associations

OMIM: gene MIM:610352 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

3 associations (top):

Study	Trait	p-value
GCST001436_4	Metabolic syndrome	4.000000e-08
GCST004785_29	Vitiligo	4.000000e-19
GCST010002_365	Refractive error	3.000000e-34

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0000195	metabolic syndrome

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL4105714 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

48 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	decreases expression, decreases methylation	3
trichostatin A	decreases expression, affects cotreatment	3
sodium arsenite	increases abundance, increases expression	2
Phenylmercuric Acetate	affects cotreatment, decreases expression	2
Valproic Acid	affects expression	2
FR900359	decreases phosphorylation	1
2,4,6-tribromophenol	decreases expression	1
triphenyl phosphate	affects expression	1
alpha-pinene	affects cotreatment, increases oxidation, increases abundance	1
decabromobiphenyl ether	decreases expression	1
afimoxifene	decreases expression, decreases reaction	1
cobaltous chloride	decreases expression	1
butyraldehyde	decreases expression	1
methacrylaldehyde	affects cotreatment, increases oxidation, increases abundance	1
beta-methylcholine	affects expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
2,2’,4,4’-tetrabromodiphenyl ether	decreases expression	1
dorsomorphin	affects cotreatment, decreases expression	1
pentabrominated diphenyl ether 100	decreases expression	1
hexabrominated diphenyl ether 153	decreases expression	1
Irinotecan	decreases expression	1
Resveratrol	affects cotreatment, increases expression	1
Sunitinib	increases expression	1
Acetaminophen	decreases expression	1
Acrolein	increases abundance, affects cotreatment, increases oxidation	1
Air Pollutants	affects cotreatment, increases abundance, increases oxidation	1
Arsenic	increases abundance, increases expression	1
Atrazine	decreases expression	1
Caffeine	decreases phosphorylation	1
Cycloheximide	affects cotreatment, increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4026813	Binding	Inhibition of human MEK2 (M1 to V400 residues) expressed in mammalian expression system assessed as percent enzyme activity at 1 uM relative to control	Identification of 4-Phenoxyquinoline Based Inhibitors for L1196M Mutant of Anaplastic Lymphoma Kinase by Structure-Based Design. — J Med Chem

Cellosaurus cell lines

1 cell lines: 1 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B2C0	Abcam HeLa PPP4R3B KO	Cancer cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.