PPP6R1

Summary

PPP6R1 (protein phosphatase 6 regulatory subunit 1, HGNC:29195) is a protein-coding gene on chromosome 19q13.42, encoding Serine/threonine-protein phosphatase 6 regulatory subunit 1 (Q9UPN7). Regulatory subunit of protein phosphatase 6 (PP6).

Protein phosphatase regulatory subunits, such as SAPS1, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. SAPS1 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C; MIM 612725) (Stefansson and Brautigan, 2006 [PubMed 16769727]).

Source: NCBI Gene 22870 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 208 total
• Druggable target: yes
• MANE Select transcript: NM_014931

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:29195
Approved symbol	PPP6R1
Name	protein phosphatase 6 regulatory subunit 1
Location	19q13.42
Locus type	gene with protein product
Status	Approved
Aliases	SAP190
Ensembl gene	ENSG00000105063
Ensembl biotype	protein_coding
OMIM	610875
Entrez	22870

Gene structure

Transcript identifiers

Ensembl transcripts: 40 — 35 protein_coding, 5 retained_intron

ENST00000412770, ENST00000586690, ENST00000587283, ENST00000587457, ENST00000589343, ENST00000591323, ENST00000591602, ENST00000592242, ENST00000875996, ENST00000875997, ENST00000875998, ENST00000875999, ENST00000876000, ENST00000876001, ENST00000876002, ENST00000876003, ENST00000876004, ENST00000876005, ENST00000938391, ENST00000938392, ENST00000938393, ENST00000938394, ENST00000938395, ENST00000938396, ENST00000938397, ENST00000938398, ENST00000938399, ENST00000938400, ENST00000938401, ENST00000938402, ENST00000961708, ENST00000961709, ENST00000961710, ENST00000961711, ENST00000961712, ENST00000961713, ENST00000961714, ENST00000961715, ENST00000961716, ENST00000961717

RefSeq mRNA: 1 — MANE Select: NM_014931 NM_014931

CCDS: CCDS46186

Canonical transcript exons

ENST00000412770 — 24 exons

Exon	Start	End
ENSE00000728070	55245120	55245185
ENSE00000728073	55242376	55242488
ENSE00000856476	55236643	55236821
ENSE00000856478	55239405	55239502
ENSE00000856479	55239594	55239683
ENSE00000856480	55239826	55239911
ENSE00000856481	55239999	55240114
ENSE00001179359	55245265	55245402
ENSE00001249731	55232075	55232211
ENSE00001432707	55246877	55247109
ENSE00001433804	55245492	55245678
ENSE00001758303	55258435	55259017
ENSE00003552148	55242166	55242279
ENSE00003558344	55231598	55231668
ENSE00003578616	55230774	55230884
ENSE00003580020	55230613	55230684
ENSE00003588416	55231410	55231491
ENSE00003597849	55229779	55230531
ENSE00003633724	55241477	55241639
ENSE00003643464	55240236	55240300
ENSE00003652072	55236913	55236970
ENSE00003653861	55240945	55241079
ENSE00003668205	55241239	55241391
ENSE00003673829	55231802	55231982

Expression profiles

Bgee: expression breadth ubiquitous, 134 present calls, max score 97.36.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 37.7630 / max 773.4901, expressed in 1821 samples.

FANTOM5 promoters (10 alternative TSS)

Promoter ID	TPM avg	Samples expressed
182767	31.2573	1816
182763	3.8269	1500
182761	0.9709	196
182766	0.6704	273
182765	0.2999	131
182764	0.2866	128
182760	0.2664	107
182759	0.1081	36
182758	0.0548	10
182757	0.0217	4

Top tissues by expression

134 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	97.36	gold quality
right testis	UBERON:0004534	97.27	gold quality
granulocyte	CL:0000094	96.95	gold quality
lymph node	UBERON:0000029	96.77	gold quality
blood	UBERON:0000178	96.45	gold quality
testis	UBERON:0000473	95.97	gold quality
bone marrow	UBERON:0002371	95.94	gold quality
vermiform appendix	UBERON:0001154	95.49	gold quality
spleen	UBERON:0002106	95.34	gold quality
bone marrow cell	CL:0002092	95.28	gold quality
leukocyte	CL:0000738	94.70	gold quality
prefrontal cortex	UBERON:0000451	94.67	gold quality
monocyte	CL:0000576	94.56	gold quality
frontal cortex	UBERON:0001870	94.42	gold quality
Ammon’s horn	UBERON:0001954	94.36	gold quality
putamen	UBERON:0001874	94.23	gold quality
anterior cingulate cortex	UBERON:0009835	94.23	gold quality
right frontal lobe	UBERON:0002810	94.18	gold quality
right uterine tube	UBERON:0001302	94.10	gold quality
temporal lobe	UBERON:0001871	94.07	gold quality
amygdala	UBERON:0001876	94.05	gold quality
superior frontal gyrus	UBERON:0002661	93.95	gold quality
cerebral cortex	UBERON:0000956	93.82	gold quality
caudate nucleus	UBERON:0001873	93.82	gold quality
lower esophagus mucosa	UBERON:0035834	93.59	gold quality
nucleus accumbens	UBERON:0001882	93.51	gold quality
small intestine	UBERON:0002108	93.18	gold quality
small intestine Peyer’s patch	UBERON:0003454	93.13	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	93.06	gold quality
right adrenal gland cortex	UBERON:0035827	93.05	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	3.43
E-MTAB-7381	no	646.25
E-CURD-89	no	126.43

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

56 targeting PPP6R1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-6873-3P	100.00	71.42	2626
HSA-MIR-7110-3P	100.00	73.18	2486
HSA-MIR-5692B	100.00	71.32	2622
HSA-MIR-5692C	100.00	71.32	2622
HSA-MIR-1277-5P	100.00	73.95	5056
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-19A-3P	99.98	75.33	2762
HSA-MIR-19B-3P	99.98	75.44	2754
HSA-MIR-185-3P	99.95	67.01	1743
HSA-MIR-450B-5P	99.92	71.48	3175
HSA-MIR-454-3P	99.91	74.01	1925
HSA-MIR-374A-5P	99.90	71.34	2923
HSA-MIR-374B-5P	99.90	69.98	2734
HSA-MIR-130A-3P	99.90	73.31	1861
HSA-MIR-130B-3P	99.90	73.27	1850
HSA-MIR-301A-3P	99.90	73.15	1839
HSA-MIR-301B-3P	99.90	73.19	1836
HSA-MIR-3666	99.90	73.24	1833
HSA-MIR-4295	99.90	73.11	1838
HSA-MIR-369-3P	99.85	70.52	2264
HSA-MIR-4447	99.85	67.81	2900
HSA-MIR-6756-5P	99.82	67.97	2466
HSA-MIR-6515-3P	99.82	68.19	1933
HSA-MIR-7110-5P	99.80	67.84	1712
HSA-MIR-6842-5P	99.80	67.54	1587
HSA-MIR-6817-3P	99.79	68.35	2126
HSA-MIR-148A-3P	99.74	73.77	1700
HSA-MIR-148B-3P	99.74	73.75	1700
HSA-MIR-152-3P	99.74	73.75	1703
HSA-MIR-4306	99.72	70.50	3630

Literature-anchored findings (GeneRIF, showing 1)

• PP6 regulatory subunit R1 is bidentate anchor for targeting protein phosphatase-6 to DNA-dependent protein kinase. (PMID:22298787)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
mus_musculus	Ppp6r1	ENSMUSG00000052296
rattus_norvegicus	Ppp6r1	ENSRNOG00000018090
drosophila_melanogaster	fmt	FBGN0035688
caenorhabditis_elegans		WBGENE00008166

Paralogs (2): PPP6R2 (ENSG00000100239), PPP6R3 (ENSG00000110075)

Protein

Protein identifiers

Serine/threonine-protein phosphatase 6 regulatory subunit 1 — Q9UPN7 (reviewed: Q9UPN7)

Alternative names: SAPS domain family member 1

All UniProt accessions (2): Q9UPN7, K7EM28

UniProt curated annotations — full annotation on UniProt →

Function. Regulatory subunit of protein phosphatase 6 (PP6). May function as a scaffolding PP6 subunit. Involved in the PP6-mediated dephosphorylation of NFKBIE opposing its degradation in response to TNF.

Subunit / interactions. Protein phosphatase 6 (PP6) holoenzyme is proposed to be a heterotrimeric complex formed of the catalytic subunit, a SAPS domain-containing subunit (PP6R) and an ankyrin repeat-domain containing regulatory subunit (ARS). Interacts with PPP6C and NFKBIE. Interacts with ANKRD28, ANKRD44 and ANKRD52.

Subcellular location. Cytoplasm.

Tissue specificity. Ubiquitous with higher expression in testis.

Similarity. Belongs to the SAPS family.

RefSeq proteins (1): NP_055746* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR007587	SAPS	Family
IPR016024	ARM-type_fold	Homologous_superfamily

Pfam: PF04499

UniProt features (18 total): modified residue 11, compositionally biased region 4, region of interest 2, chain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9UPN7-F1	67.96	0.43

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (11): 530, 531, 635, 638, 702, 726, 759, 846, 232, 524, 529

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-204005	COPII-mediated vesicle transport

MSigDB gene sets: 139 (showing top): TATTATA_MIR374, REACTOME_MEMBRANE_TRAFFICKING, GOMF_GTPASE_BINDING, TTTGCAC_MIR19A_MIR19B, GOCC_LAMELLIPODIUM, GOMF_PROTEIN_PHOSPHATASE_BINDING, GOCC_CELL_LEADING_EDGE, GOMF_PHOSPHATASE_BINDING, GOMF_ENZYME_REGULATOR_ACTIVITY, DODD_NASOPHARYNGEAL_CARCINOMA_DN, GOMF_PHOSPHATASE_REGULATOR_ACTIVITY, REACTOME_POST_TRANSLATIONAL_PROTEIN_MODIFICATION, PURBEY_TARGETS_OF_CTBP1_NOT_SATB1_UP, GHANDHI_DIRECT_IRRADIATION_UP, REACTOME_ER_TO_GOLGI_ANTEROGRADE_TRANSPORT

GO Biological Process (1): regulation of signal transduction (GO:0009966)

GO Molecular Function (4): protein phosphatase regulator activity (GO:0019888), protein phosphatase binding (GO:0019903), small GTPase binding (GO:0031267), protein binding (GO:0005515)

GO Cellular Component (4): nucleus (GO:0005634), cytosol (GO:0005829), lamellipodium (GO:0030027), cytoplasm (GO:0005737)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
ER to Golgi Anterograde Transport	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
signal transduction	1
regulation of cell communication	1
regulation of signaling	1
regulation of response to stimulus	1
phosphoprotein phosphatase activity	1
phosphatase regulator activity	1
protein phosphatase binding	1
phosphatase binding	1
GTPase binding	1
binding	1
intracellular membrane-bounded organelle	1
cytoplasm	1
cell leading edge	1
plasma membrane bounded cell projection	1
intracellular anatomical structure	1

Protein interactions and networks

STRING

1268 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
PPP6R1	PPP6C	O00743	992
PPP6R1	SF3B1	O75533	924
PPP6R1	ANKRD28	O15084	853
PPP6R1	NFKBIE	O00221	838
PPP6R1	ANKRD44	Q8N8A2	774
PPP6R1	PPP2CA	P05323	764
PPP6R1	ANKRD52	Q8NB46	683
PPP6R1	DPH3	Q96FX2	683
PPP6R1	KTI12	Q96EK9	637
PPP6R1	PPP4C	P33172	620
PPP6R1	ELP6	Q0PNE2	551
PPP6R1	PPP4R2	Q9NY27	519
PPP6R1	PPP4R4	Q6NUP7	518
PPP6R1	PRKDC	P78527	510
PPP6R1	ZNF579	Q8NAF0	504

IntAct

195 interactions, top by confidence:

A	B	Type	Score
PPP6C	PPP6R1	psi-mi:“MI:0915”(physical association)	0.920
PPP6R1	PPP6C	psi-mi:“MI:0914”(association)	0.920
PIK3CA	PIK3R2	psi-mi:“MI:0914”(association)	0.900
PPP6C	ANKRD28	psi-mi:“MI:0914”(association)	0.870
ANKRD28	PPP6C	psi-mi:“MI:0914”(association)	0.870
PPP6R1	ANKRD44	psi-mi:“MI:0914”(association)	0.800
ANKRD52	PPP6C	psi-mi:“MI:0914”(association)	0.790
ANKRD44	PPP6C	psi-mi:“MI:0914”(association)	0.790
ANKRD28	PPP6R1	psi-mi:“MI:0914”(association)	0.750
ANKRD28	PPP6R1	psi-mi:“MI:0915”(physical association)	0.750
PPP6R1	ANKRD28	psi-mi:“MI:0915”(physical association)	0.750
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710

BioGRID (253): PPP6R1 (Affinity Capture-MS), PPP6R1 (Affinity Capture-MS), PPP6R1 (Affinity Capture-MS), PPP6R1 (Affinity Capture-MS), PPP6R1 (Affinity Capture-MS), PPP6R1 (Affinity Capture-MS), PPP6R1 (Proximity Label-MS), PPP6R1 (Affinity Capture-MS), PPP6R1 (Affinity Capture-MS), PPP6R1 (Affinity Capture-MS), PPP6R1 (Affinity Capture-MS), PPP6R1 (Affinity Capture-MS), PPP6R1 (Affinity Capture-MS), PPP6R1 (Proximity Label-MS), PPP6R1 (Affinity Capture-MS)

ESM2 similar proteins: A0A571BF63, A0A8M9QN10, A1L1K1, A1YER0, A2ARM1, A2AVJ5, A2CI98, A2CJ06, A2D5H2, A7YY97, B5DEB9, C9JE40, O00443, O43147, O43149, O93307, O95475, P59729, Q15475, Q2KHT9, Q32N92, Q4V7K4, Q5E9R0, Q5EB20, Q5PQS0, Q5R6T6, Q5RAY1, Q5RJH6, Q5U464, Q61194, Q62231, Q6AW68, Q6DGF9, Q6MZQ0, Q6TNU3, Q6ZUJ8, Q7TSI1, Q7TSI3, Q7Z3E5, Q80U12

Diamond homologs: O75170, Q5F471, Q5H9R7, Q6NRF1, Q6NRI0, Q7TSI3, Q8R3Q2, Q922D4, Q9UPN7, B5VMH6, C8ZCJ2, P36123, P40856

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 195 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Lysosphingolipid and LPA receptors	5	30.4×	3e-04

Disease & clinical

Clinical variants and AI predictions

ClinVar

208 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	162
Likely benign	7
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

4283 predictions. Top by Δscore:

Variant	Effect	Δscore
19:55230611:A:AC	donor_gain	1.0000
19:55230612:C:CC	donor_gain	1.0000
19:55230769:CTCA:C	donor_loss	1.0000
19:55230770:TCAC:T	donor_loss	1.0000
19:55230771:CACCT:C	donor_loss	1.0000
19:55230772:A:AC	donor_gain	1.0000
19:55230773:C:A	donor_loss	1.0000
19:55230773:C:CC	donor_gain	1.0000
19:55230773:CCT:C	donor_gain	1.0000
19:55230880:TTGCA:T	acceptor_gain	1.0000
19:55230881:TGCA:T	acceptor_gain	1.0000
19:55230883:CA:C	acceptor_gain	1.0000
19:55230883:CACTG:C	acceptor_loss	1.0000
19:55230885:C:CA	acceptor_loss	1.0000
19:55230885:C:CC	acceptor_gain	1.0000
19:55231487:AGGGG:A	acceptor_gain	1.0000
19:55231488:GGGG:G	acceptor_gain	1.0000
19:55231489:GGG:G	acceptor_gain	1.0000
19:55231490:GG:G	acceptor_gain	1.0000
19:55231492:C:CC	acceptor_gain	1.0000
19:55231797:CTCA:C	donor_loss	1.0000
19:55231798:TCAC:T	donor_loss	1.0000
19:55231800:A:T	donor_loss	1.0000
19:55231801:CCT:C	donor_loss	1.0000
19:55236641:A:AC	donor_gain	1.0000
19:55236642:C:CC	donor_gain	1.0000
19:55236723:C:A	donor_gain	1.0000
19:55236818:TGGG:T	acceptor_gain	1.0000
19:55236911:A:AC	donor_gain	1.0000
19:55236911:ACGTT:A	donor_gain	1.0000

AlphaMissense

5798 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:55245323:A:G	L165P	0.999
19:55245326:T:G	D164A	0.999
19:55245335:G:T	A161D	0.999
19:55245528:C:A	K126N	0.999
19:55245528:C:G	K126N	0.999
19:55245531:G:C	S125R	0.999
19:55245531:G:T	S125R	0.999
19:55245533:T:G	S125R	0.999
19:55245537:G:C	F123L	0.999
19:55245537:G:T	F123L	0.999
19:55245539:A:G	F123L	0.999
19:55245540:G:C	S122R	0.999
19:55245540:G:T	S122R	0.999
19:55245542:T:G	S122R	0.999
19:55245652:A:G	L85P	0.999
19:55245652:A:T	L85Q	0.999
19:55245664:G:T	A81D	0.999
19:55247095:C:A	W3C	0.999
19:55247095:C:G	W3C	0.999
19:55247097:A:G	W3R	0.999
19:55247097:A:T	W3R	0.999
19:55247098:A:C	F2L	0.999
19:55247098:A:T	F2L	0.999
19:55247100:A:G	F2L	0.999
19:55245181:A:G	L186P	0.998
19:55245326:T:C	D164G	0.998
19:55245327:C:G	D164H	0.998
19:55245530:T:C	K126E	0.998
19:55245544:G:T	A121D	0.998
19:55245652:A:C	L85R	0.998

dbSNP variants (sampled 300 via entrez): RS1000127376 (19:55232946 C>T), RS1000142373 (19:55257063 T>A,C), RS1000146061 (19:55247819 A>G), RS1000312501 (19:55238692 C>T), RS1000342168 (19:55238959 T>C), RS1000460725 (19:55234025 C>T), RS1000467452 (19:55255898 A>G), RS1000499245 (19:55253535 C>T), RS1000646035 (19:55234238 T>C), RS1000668015 (19:55230441 G>A), RS1000730868 (19:55234202 C>T), RS1000817228 (19:55248797 A>C,T), RS1000883294 (19:55253818 G>A), RS1000920777 (19:55242099 G>A), RS1000932862 (19:55249038 G>A)

Disease associations

OMIM: gene MIM:610875 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST010653_3	Thyroid stimulating hormone levels	1.000000e-08
GCST011739_12	Cutaneous leishmaniasis	5.000000e-06
GCST90002390_663	Mean corpuscular hemoglobin	3.000000e-12
GCST90002392_79	Mean corpuscular volume	6.000000e-09

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0004527	mean corpuscular hemoglobin

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL4105774 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 4 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
8.85	Kd	1.421	nM	CHEMBL5653589
8.85	ED50	1.421	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 6 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149058: Binding affinity to human PPP6R1 incubated for 45 mins by Kinobead based pull down assay	kd	0.0014	uM

CTD chemical–gene interactions

43 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	decreases expression, increases expression	2
Air Pollutants	affects cotreatment, increases abundance, increases oxidation, decreases expression	2
aristolochic acid I	increases expression	1
FR900359	increases phosphorylation	1
dicrotophos	increases expression	1
2,4,6-tribromophenol	decreases expression	1
alpha-pinene	increases abundance, affects cotreatment, increases oxidation	1
bisphenol A	decreases expression	1
sodium arsenate	decreases expression	1
decabromobiphenyl ether	decreases expression	1
beta-lapachone	decreases expression	1
cobaltous chloride	increases expression	1
tetrabromobisphenol A	decreases expression	1
benzo(e)pyrene	decreases methylation	1
cupric chloride	increases expression	1
methacrylaldehyde	affects cotreatment, increases oxidation, increases abundance	1
di-n-butylphosphoric acid	affects expression	1
CGP 52608	affects binding, increases reaction	1
2-palmitoylglycerol	increases expression	1
ICG 001	decreases expression	1
2,2’,4,4’-tetrabromodiphenyl ether	decreases expression	1
pentabrominated diphenyl ether 100	decreases expression	1
hexabrominated diphenyl ether 153	decreases expression	1
4-(4-((5-(4,5-dimethyl-2-nitrophenyl)-2-furanyl)methylene)-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl)benzoic acid	decreases expression	1
Temozolomide	decreases expression	1
Acrolein	affects cotreatment, increases oxidation, increases abundance	1
Estradiol	affects expression	1
Lead	decreases expression	1
Methapyrilene	decreases methylation	1
Nickel	increases expression	1

ChEMBL screening assays

2 unique, capped per target: 2 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4012582	Binding	Binding affinity to PP6R1 protein in human INA-6 cells after 3 hrs by nanoLC-MS/MS method	Ugi Reaction-Derived α-Acyl Aminocarboxamides Bind to Phosphatidylinositol 3-Kinase-Related Kinases, Inhibit HSF1-Dependent Heat Shock Response, and Induce Apoptosis in Multiple Myeloma Cells. — J Med Chem

Cellosaurus cell lines

3 cell lines: 2 cancer cell line, 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B3EN	Abcam HEK293T PPP6R1 KO	Transformed cell line	Female
CVCL_TG30	HAP1 PPP6R1 (-) 1	Cancer cell line	Male
CVCL_TG31	HAP1 PPP6R1 (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.