QTRT2

Summary

QTRT2 (queuine tRNA-ribosyltransferase accessory subunit 2, HGNC:25771) is a protein-coding gene on chromosome 3q13.31, encoding Queuine tRNA-ribosyltransferase accessory subunit 2 (Q9H974). Non-catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified….

This gene encodes a subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine, and tyrosine. The encoded protein may play a role in the queuosine 5’-monophosphate salvage pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Source: NCBI Gene 79691 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 21 total
• Druggable target: yes
• MANE Select transcript: NM_024638

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:25771
Approved symbol	QTRT2
Name	queuine tRNA-ribosyltransferase accessory subunit 2
Location	3q13.31
Locus type	gene with protein product
Status	Approved
Aliases	FLJ12960
Ensembl gene	ENSG00000151576
Ensembl biotype	protein_coding
Entrez	79691

Gene structure

Transcript identifiers

Ensembl transcripts: 21 — 12 protein_coding, 7 retained_intron, 2 protein_coding_CDS_not_defined

ENST00000281273, ENST00000460163, ENST00000462869, ENST00000462966, ENST00000466050, ENST00000466870, ENST00000467703, ENST00000472599, ENST00000479882, ENST00000482307, ENST00000483272, ENST00000485050, ENST00000488665, ENST00000490183, ENST00000493014, ENST00000495782, ENST00000929108, ENST00000929109, ENST00000929110, ENST00000929111, ENST00000949860

RefSeq mRNA: 4 — MANE Select: NM_024638 NM_001256835, NM_001256836, NM_001256837, NM_024638

CCDS: CCDS33828, CCDS58844, CCDS58845, CCDS58846

Canonical transcript exons

ENST00000281273 — 10 exons

Exon	Start	End
ENSE00001000445	114056999	114057106
ENSE00001000452	114067987	114068063
ENSE00001194849	114085673	114088422
ENSE00001507536	114076743	114076942
ENSE00001813676	114056737	114056864
ENSE00003458665	114079906	114080057
ENSE00003466499	114082677	114082794
ENSE00003491328	114070626	114070838
ENSE00003529015	114066228	114066283
ENSE00003670031	114065237	114065457

Expression profiles

Bgee: expression breadth ubiquitous, 250 present calls, max score 91.89.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 14.7978 / max 263.5127, expressed in 1781 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
37988	11.7463	1763
37987	3.0515	1368

Top tissues by expression

275 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adrenal tissue	UBERON:0018303	91.89	gold quality
secondary oocyte	CL:0000655	88.82	gold quality
monocyte	CL:0000576	88.73	gold quality
mononuclear cell	CL:0000842	88.54	gold quality
leukocyte	CL:0000738	88.21	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	87.99	gold quality
colonic epithelium	UBERON:0000397	87.21	gold quality
calcaneal tendon	UBERON:0003701	86.44	gold quality
oocyte	CL:0000023	85.52	gold quality
bone marrow cell	CL:0002092	85.01	gold quality
rectum	UBERON:0001052	84.41	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	83.66	gold quality
ventricular zone	UBERON:0003053	83.30	gold quality
right uterine tube	UBERON:0001302	83.29	gold quality
tonsil	UBERON:0002372	82.56	gold quality
granulocyte	CL:0000094	82.54	gold quality
islet of Langerhans	UBERON:0000006	82.53	gold quality
vermiform appendix	UBERON:0001154	82.27	gold quality
lymph node	UBERON:0000029	81.92	gold quality
adrenal gland	UBERON:0002369	81.43	gold quality
pancreas	UBERON:0001264	81.37	gold quality
esophagus squamous epithelium	UBERON:0006920	81.34	gold quality
body of pancreas	UBERON:0001150	81.30	gold quality
right adrenal gland	UBERON:0001233	81.14	gold quality
right adrenal gland cortex	UBERON:0035827	81.14	gold quality
upper lobe of left lung	UBERON:0008952	81.04	gold quality
right lobe of thyroid gland	UBERON:0001119	80.98	gold quality
left adrenal gland cortex	UBERON:0035825	80.90	gold quality
spleen	UBERON:0002106	80.87	gold quality
gall bladder	UBERON:0002110	80.87	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	4.80

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

98 targeting QTRT2, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-5692A	100.00	74.40	6850
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-6748-5P	100.00	65.81	1057
HSA-MIR-6851-5P	100.00	65.63	1294
HSA-MIR-3689D	100.00	66.14	1181
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-6077	99.99	68.04	2299
HSA-MIR-513B-5P	99.99	69.96	2150
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-371B-5P	99.99	75.34	4759
HSA-MIR-373-5P	99.98	75.36	4753
HSA-MIR-616-5P	99.98	75.58	4775
HSA-MIR-548P	99.98	72.25	3784
HSA-MIR-3688-3P	99.97	72.02	2834
HSA-MIR-548AJ-3P	99.96	73.38	5345
HSA-MIR-548X-3P	99.96	73.38	5345
HSA-MIR-9-3P	99.96	70.88	2068
HSA-MIR-548AA	99.96	70.64	3753
HSA-MIR-548AP-3P	99.96	70.64	3753
HSA-MIR-548T-3P	99.96	70.64	3753
HSA-MIR-548J-3P	99.94	72.61	4881
HSA-MIR-651-3P	99.94	73.48	5177
HSA-MIR-548AE-3P	99.93	72.66	4867
HSA-MIR-548AH-3P	99.93	72.54	4872
HSA-MIR-548AM-3P	99.93	72.54	4872
HSA-MIR-548AQ-3P	99.93	72.66	4867
HSA-MIR-6721-5P	99.93	68.92	2981
HSA-MIR-4778-3P	99.93	70.40	1818
HSA-MIR-3529-3P	99.90	73.55	3045
HSA-MIR-4302	99.89	67.94	1187

Literature-anchored findings (GeneRIF, showing 1)

• TGT is composed of a catalytic subunit, QTRT1, and QTRTD1, not USP14. QTRTD1 has been implicated as the salvage enzyme that generates free queuine from QMP. (PMID:20354154)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	qtrt2	ENSDARG00000016093
mus_musculus	Qtrt2	ENSMUSG00000022704
rattus_norvegicus	Qtrt2	ENSRNOG00000060729
drosophila_melanogaster	CG3434	FBGN0036000
caenorhabditis_elegans		WBGENE00006567

Paralogs (1): QTRT1 (ENSG00000213339)

Protein

Protein identifiers

Queuine tRNA-ribosyltransferase accessory subunit 2 — Q9H974 (reviewed: Q9H974)

Alternative names: Queuine tRNA-ribosyltransferase domain-containing protein 1

All UniProt accessions (4): Q9H974, A0A1D5RMQ5, C9JJ71, C9K0B6

UniProt curated annotations — full annotation on UniProt →

Function. Non-catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).

Subunit / interactions. Heterodimer of a catalytic subunit QTRT1 and an accessory subunit QTRT2.

Subcellular location. Cytoplasm. Mitochondrion outer membrane.

Cofactor. Binds 1 zinc ion per subunit.

Similarity. Belongs to the queuine tRNA-ribosyltransferase family. QTRT2 subfamily.

Isoforms (4)

UniProt ID	Names	Canonical?
Q9H974-1	1	yes
Q9H974-2	2
Q9H974-3	3
Q9H974-4	4

RefSeq proteins (4): NP_001243764, NP_001243765, NP_001243766, NP_078914* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR002616	tRNA_ribo_trans-like	Domain
IPR028592	QTRTD1	Family
IPR036511	TGT-like_sf	Homologous_superfamily
IPR050852	Queuine_tRNA-ribosyltrfase	Family

Pfam: PF01702

Enzyme classification (BRENDA):

• EC 2.4.2.64 — tRNA-guanosine34 queuine transglycosylase (BRENDA: 15 organisms, 38 substrates, 46 inhibitors, 29 Km, 21 kcat entries)

Substrate kinetics (BRENDA)

8 substrates with measured Km, best-characterized 8. Km ranges are aggregated across organisms/conditions.

Substrate	Km (mM)	Measurements
GUANINE	0.0001–0.105	12
QUEUINE	0.0003–0.0023	5
XANTHINE	0.0026–0.224	3
7-AMINOMETHYL-7-CARBAGUANINE	0.0223–0.132	2
GUANINE34 IN TRNA	0.0004–0.001	2
HYPOXANTHINE	0.49–1.55	2
7-(AMINOMETHYL)-7-DEAZAGUANINE	0.0021	1
GUANINE34 IN TRNAASP	0.003	1

UniProt features (52 total): helix 18, strand 17, mutagenesis site 7, binding site 4, splice variant 3, chain 1, sequence conflict 1, turn 1

Structure

Experimental structures (PDB)

2 structures.

PDB	Method	Resolution (Å)
7NQ4	X-RAY DIFFRACTION	2.88
8OMR	ELECTRON MICROSCOPY	3.3

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9H974-F1	90.05	0.81

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (4): 351; 353; 356; 382

Mutagenesis-validated functional residues (7):

Position	Phenotype
121	decreased affinity for trnas, leading to decreased formation of hypermodified nucleoside queuosine trna.
152	does not significantly affect ability to promote formation of hypermodified nucleoside queuosine trna.
158–161	decreased affinity for trnas.
158	does not significantly affect ability to promote formation of hypermodified nucleoside queuosine trna.
161	does not significantly affect ability to promote formation of hypermodified nucleoside queuosine trna.
116	does not significantly affect ability to promote formation of hypermodified nucleoside queuosine trna.
118	does not significantly affect ability to promote formation of hypermodified nucleoside queuosine trna.

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-6782315	tRNA modification in the nucleus and cytosol

MSigDB gene sets: 167 (showing top): GOBP_TRNA_METABOLIC_PROCESS, GRAESSMANN_APOPTOSIS_BY_SERUM_DEPRIVATION_UP, CAGCTG_AP4_Q5, AACWWCAANK_UNKNOWN, GOBP_RNA_MODIFICATION, MYOD_01, MYCMAX_01, GOCC_MITOCHONDRIAL_ENVELOPE, GARY_CD5_TARGETS_DN, DODD_NASOPHARYNGEAL_CARCINOMA_UP, GOBP_TRNA_PROCESSING, REACTOME_METABOLISM_OF_RNA, GOBP_TRNA_MODIFICATION, TGGAAA_NFAT_Q4_01, GOCC_CYTOPLASMIC_SIDE_OF_MEMBRANE

GO Biological Process (4): tRNA wobble guanine modification (GO:0002099), tRNA modification (GO:0006400), tRNA processing (GO:0008033), obsolete tRNA-guanine transglycosylation (GO:0101030)

GO Molecular Function (7): tRNA binding (GO:0000049), tRNA-guanosine(34) queuine transglycosylase activity (GO:0008479), protein homodimerization activity (GO:0042803), metal ion binding (GO:0046872), protein heterodimerization activity (GO:0046982), enzyme-substrate adaptor activity (GO:0140767), protein binding (GO:0005515)

GO Cellular Component (8): cytoplasm (GO:0005737), mitochondrion (GO:0005739), mitochondrial outer membrane (GO:0005741), cytoplasmic side of mitochondrial outer membrane (GO:0032473), tRNA-guanine transglycosylase complex (GO:0120507), transferase complex (GO:1990234), membrane (GO:0016020), protein-containing complex (GO:0032991)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
tRNA processing	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
protein dimerization activity	2
cellular anatomical structure	2
tRNA wobble base modification	1
tRNA processing	1
RNA modification	1
RNA processing	1
tRNA metabolic process	1
RNA binding	1
pentosyltransferase activity	1
catalytic activity, acting on a tRNA	1
identical protein binding	1
cation binding	1
protein-macromolecule adaptor activity	1
binding	1
intracellular anatomical structure	1
cytoplasm	1
intracellular membrane-bounded organelle	1
mitochondrial membrane	1
organelle outer membrane	1
mitochondrial outer membrane	1
cytoplasmic side of membrane	1
transferase complex	1
catalytic complex	1
cellular_component	1

Protein interactions and networks

STRING

1260 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
QTRT2	QTRT1	Q9BXR0	894
QTRT2	TRDMT1	O14717	642
QTRT2	GTPBP3	Q969Y2	625
QTRT2	TRIT1	Q9H3H1	541
QTRT2	TRMU	O75648	538
QTRT2	PUS3	Q9BZE2	519
QTRT2	TRMT5	Q32P41	505
QTRT2	YRDC	Q86U90	495
QTRT2	CDK5RAP1	Q96SZ6	485
QTRT2	MTO1	Q9Y2Z2	481
QTRT2	NSUN3	Q9H649	478
QTRT2	PRR29	P0C7W0	476
QTRT2	NSUN2	Q08J23	464
QTRT2	CTU1	Q7Z7A3	464
QTRT2	RPUSD2	Q8IZ73	456

IntAct

27 interactions, top by confidence:

A	B	Type	Score
QTRT2	QTRT1	psi-mi:“MI:0407”(direct interaction)	0.560
THUMPD1	YBX1	psi-mi:“MI:0914”(association)	0.530
DOK3	TPP2	psi-mi:“MI:0914”(association)	0.530
IGHA1	PLG	psi-mi:“MI:0914”(association)	0.350
IGHG1	PDPK1	psi-mi:“MI:0914”(association)	0.350
ALB	CNOT1	psi-mi:“MI:0914”(association)	0.350
ALB	SH3BP5	psi-mi:“MI:0914”(association)	0.350
PB2	ESYT2	psi-mi:“MI:0914”(association)	0.350
HSCB	RBP5	psi-mi:“MI:0914”(association)	0.350
P2RY6	RAVER1	psi-mi:“MI:0914”(association)	0.350
M		psi-mi:“MI:0914”(association)	0.350
ATG16L1	ESYT2	psi-mi:“MI:0914”(association)	0.350
DOK3	BLTP3B	psi-mi:“MI:0914”(association)	0.350
TFPT	KRBA1	psi-mi:“MI:0914”(association)	0.350
SNX21	ACOT8	psi-mi:“MI:0914”(association)	0.350
TFPT	CCP110	psi-mi:“MI:0914”(association)	0.350
DOK3	HSPA8	psi-mi:“MI:0914”(association)	0.350
THUMPD1	H2AX	psi-mi:“MI:0914”(association)	0.350
SPHK1	ALDH3B1	psi-mi:“MI:0914”(association)	0.350
QTRT2	PGM3	psi-mi:“MI:0915”(physical association)	0.000
QTRT2	BDH1	psi-mi:“MI:0915”(physical association)	0.000
QTRT2	MTARC1	psi-mi:“MI:0915”(physical association)	0.000
CLU	QTRT2	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (43): QTRT1 (Co-fractionation), MARC1 (Affinity Capture-MS), PGM3 (Affinity Capture-MS), BDH1 (Affinity Capture-MS), QTRTD1 (Affinity Capture-MS), QTRTD1 (Affinity Capture-RNA), QTRTD1 (Affinity Capture-MS), QTRTD1 (Affinity Capture-MS), QTRTD1 (Affinity Capture-MS), QTRTD1 (Affinity Capture-MS), QTRTD1 (Proximity Label-MS), QTRTD1 (Affinity Capture-MS), QTRTD1 (Affinity Capture-MS), QTRTD1 (Proximity Label-MS), QTRTD1 (Affinity Capture-MS)

ESM2 similar proteins: A0AVT1, A1L1C5, A1L259, A2RTX5, A3KMX8, A5PJD0, A6H630, A6NGE7, B8ZXI1, O60678, O70467, O75879, P32455, P32456, Q0V9S0, Q0ZDF7, Q22017, Q283N4, Q2KHV5, Q4R526, Q4R646, Q58EM4, Q5D1D6, Q5R998, Q5RBE1, Q5ZIE6, Q61107, Q68EH8, Q6AXB1, Q6AYT5, Q6DJ95, Q6DJA3, Q6ING7, Q6NTW6, Q6PA41, Q6ZN66, Q7SXP2, Q7T010, Q8BIJ6, Q8BLY2

Diamond homologs: A1TVN0, A1VJ15, A1WCK2, A2RHN5, A2SM97, A3N087, A4IYF8, A4J541, A5D3G6, A5IM22, A6Q906, A7NBL6, A8FFQ9, A8GAM5, A8GUA5, A8ZRX7, A9HBJ2, A9KHU4, B0BP03, B0K0M1, B0K959, B0WAN0, B1I4K8, B1LB70, B1XWG6, B2SHD4, B3GXF8, B3MA91, B3NCH1, B4H1X9, B4HL48, B4IXD3, B4KXI8, B4LFW2, B4N549, B4PEV9, B7Q5K1, B8I6M0, B8ZXI1, B9E716

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

21 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	4
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

3233 predictions. Top by Δscore:

Variant	Effect	Δscore
3:114008494:A:C	donor_gain	1.0000
3:114018688:C:A	donor_gain	1.0000
3:114018867:CA:C	acceptor_gain	1.0000
3:114018869:C:CC	acceptor_gain	1.0000
3:114034920:CTTA:C	donor_loss	1.0000
3:114034921:TTA:T	donor_loss	1.0000
3:114034922:TAC:T	donor_loss	1.0000
3:114034923:A:AC	donor_gain	1.0000
3:114034923:A:AG	donor_loss	1.0000
3:114034924:C:CC	donor_gain	1.0000
3:114034924:CA:C	donor_gain	1.0000
3:114035006:T:TA	donor_gain	1.0000
3:114035147:ACC:A	acceptor_loss	1.0000
3:114036571:T:TA	donor_gain	1.0000
3:114036604:CCA:C	donor_loss	1.0000
3:114036605:CA:C	donor_loss	1.0000
3:114036606:ACCT:A	donor_loss	1.0000
3:114036607:CC:C	donor_loss	1.0000
3:114036607:CCTG:C	donor_gain	1.0000
3:114036621:ATCT:A	donor_gain	1.0000
3:114036622:T:C	donor_gain	1.0000
3:114036782:TAAAT:T	acceptor_gain	1.0000
3:114036783:AAAT:A	acceptor_gain	1.0000
3:114036784:AAT:A	acceptor_gain	1.0000
3:114036785:AT:A	acceptor_gain	1.0000
3:114036786:TC:T	acceptor_loss	1.0000
3:114036787:C:A	acceptor_loss	1.0000
3:114036787:C:CC	acceptor_gain	1.0000
3:114036797:C:CT	acceptor_gain	1.0000
3:114036798:A:T	acceptor_gain	1.0000

AlphaMissense

2704 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
3:114079976:A:C	S273R	0.995
3:114079978:T:A	S273R	0.995
3:114079978:T:G	S273R	0.995
3:114070789:G:C	R166P	0.992
3:114076845:T:C	F217L	0.991
3:114076847:C:A	F217L	0.991
3:114076847:C:G	F217L	0.991
3:114085805:C:A	N383K	0.989
3:114085805:C:G	N383K	0.989
3:114065391:T:C	L45P	0.988
3:114076846:T:C	F217S	0.987
3:114085756:T:C	L367P	0.987
3:114080013:C:A	A285D	0.985
3:114076809:T:C	S205P	0.982
3:114076924:T:C	L243P	0.982
3:114076768:G:A	G191E	0.981
3:114076843:G:A	G216D	0.981
3:114065294:C:A	R13S	0.980
3:114076812:G:C	A206P	0.980
3:114070783:T:A	V164D	0.979
3:114079906:G:C	R249S	0.978
3:114079906:G:T	R249S	0.978
3:114065354:T:C	C33R	0.977
3:114076831:G:C	R212P	0.977
3:114085771:A:T	E372V	0.977
3:114076779:G:C	G195R	0.976
3:114079964:G:C	D269H	0.975
3:114080016:T:C	L286P	0.975
3:114076842:G:C	G216R	0.974
3:114085724:T:G	C356W	0.974

dbSNP variants (sampled 300 via entrez): RS1000030638 (3:114070244 A>G), RS1000304967 (3:114064848 A>G), RS1000527576 (3:114075351 C>T), RS1000602419 (3:114056069 A>G), RS1000639549 (3:114075521 T>C,G), RS1000769649 (3:114081380 C>G), RS1000952066 (3:114054738 A>C), RS1001131673 (3:114080787 A>C,G), RS1001135552 (3:114069072 T>A), RS1001310084 (3:114087538 C>A), RS1001394299 (3:114055050 C>T), RS1001780835 (3:114068583 G>A,T), RS1001782142 (3:114079805 T>C), RS1001794027 (3:114060313 T>C), RS1001833422 (3:114069026 C>G,T)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067252 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
9.39	Kd	0.411	nM	CHEMBL5653589
9.04	ED50	0.907	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149166: Binding affinity to human QTRTD1 incubated for 45 mins by Kinobead based pull down assay	kd	0.0004	uM

CTD chemical–gene interactions

29 total (human), top 29 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
trichostatin A	affects expression	1
sodium arsenite	decreases expression	1
CGP 52608	affects binding, increases reaction	1
bisphenol S	decreases methylation	1
jinfukang	affects cotreatment, decreases expression	1
Resveratrol	affects cotreatment, increases expression	1
Sunitinib	increases expression	1
Acetaminophen	decreases expression	1
Arsenic	affects methylation	1
Benzo(a)pyrene	affects methylation	1
Cannabidiol	affects cotreatment, decreases expression	1
Cisplatin	affects cotreatment, decreases expression	1
Cuprizone	affects cotreatment, decreases expression	1
Dichlorodiphenyl Dichloroethylene	increases expression	1
Dietary Carbohydrates	increases expression	1
Diethylstilbestrol	increases expression	1
Doxorubicin	decreases expression	1
Enzyme Inhibitors	decreases activity, increases O-linked glycosylation	1
Estradiol	increases expression	1
Ethyl Methanesulfonate	decreases expression	1
Ivermectin	decreases expression	1
Lead	affects expression	1
Plant Extracts	affects cotreatment, increases expression	1
Thiram	decreases expression	1
Tobacco Smoke Pollution	increases expression	1
Valproic Acid	affects expression	1
Cyclosporine	increases expression	1
Aflatoxin B1	increases methylation	1
Cadmium Chloride	decreases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652208	Binding	Binding affinity to human QTRTD1 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.