RCN1

Summary

RCN1 (reticulocalbin 1, HGNC:9934) is a protein-coding gene on chromosome 11p13, encoding Reticulocalbin-1 (Q15293). May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.

Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.

Source: NCBI Gene 5954 — RefSeq curated summary.

At a glance

• GWAS associations: 8
• Clinical variants (ClinVar): 53 total
• Druggable target: yes
• MANE Select transcript: NM_002901

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:9934
Approved symbol	RCN1
Name	reticulocalbin 1
Location	11p13
Locus type	gene with protein product
Status	Approved
Aliases	Rcal, PIG20, FLJ37041
Ensembl gene	ENSG00000049449
Ensembl biotype	protein_coding
OMIM	602735
Entrez	5954

Gene structure

Transcript identifiers

Ensembl transcripts: 10 — 5 protein_coding, 2 nonsense_mediated_decay, 2 retained_intron, 1 protein_coding_CDS_not_defined

ENST00000054950, ENST00000527337, ENST00000528630, ENST00000531345, ENST00000532474, ENST00000532721, ENST00000533898, ENST00000877959, ENST00000877960, ENST00000972102

RefSeq mRNA: 1 — MANE Select: NM_002901 NM_002901

CCDS: CCDS7876

Canonical transcript exons

ENST00000054950 — 6 exons

Exon	Start	End
ENSE00001211610	32104365	32105722
ENSE00001370613	32091074	32091450
ENSE00003815346	32098350	32098528
ENSE00003817177	32097144	32097337
ENSE00003820634	32100548	32100608
ENSE00003824695	32103281	32103480

Expression profiles

Bgee: expression breadth ubiquitous, 291 present calls, max score 99.35.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 75.2247 / max 934.8261, expressed in 1808 samples.

FANTOM5 promoters (9 alternative TSS)

Promoter ID	TPM avg	Samples expressed
113616	35.6192	1704
113617	12.5551	1578
113612	9.5281	1458
113618	6.5273	1614
113615	4.4929	1271
113613	2.4284	1146
113611	2.2477	1206
113614	1.0316	738
113619	0.7944	436

Top tissues by expression

296 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
corpus epididymis	UBERON:0004359	99.35	gold quality
stromal cell of endometrium	CL:0002255	99.22	gold quality
ventricular zone	UBERON:0003053	98.91	gold quality
cartilage tissue	UBERON:0002418	98.45	gold quality
periodontal ligament	UBERON:0008266	98.30	gold quality
endometrium	UBERON:0001295	98.12	gold quality
seminal vesicle	UBERON:0000998	98.00	gold quality
tendon of biceps brachii	UBERON:0008188	97.94	gold quality
right coronary artery	UBERON:0001625	97.51	gold quality
tibia	UBERON:0000979	97.29	gold quality
caput epididymis	UBERON:0004358	97.25	gold quality
left coronary artery	UBERON:0001626	97.20	gold quality
adenohypophysis	UBERON:0002196	97.15	gold quality
placenta	UBERON:0001987	97.10	gold quality
omental fat pad	UBERON:0010414	97.09	gold quality
coronary artery	UBERON:0001621	97.08	gold quality
peritoneum	UBERON:0002358	97.08	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	97.01	gold quality
pituitary gland	UBERON:0000007	96.96	gold quality
right ovary	UBERON:0002118	96.96	gold quality
adipose tissue of abdominal region	UBERON:0007808	96.90	gold quality
left ovary	UBERON:0002119	96.64	gold quality
colonic epithelium	UBERON:0000397	96.63	gold quality
ganglionic eminence	UBERON:0004023	96.63	gold quality
ovary	UBERON:0000992	96.56	gold quality
smooth muscle tissue	UBERON:0001135	96.53	gold quality
islet of Langerhans	UBERON:0000006	96.51	gold quality
ascending aorta	UBERON:0001496	96.50	gold quality
prostate gland	UBERON:0002367	96.48	gold quality
thoracic aorta	UBERON:0001515	96.47	gold quality

Single-cell (SCXA)

Detected in 13 experiment(s), a significant marker in 11.

Experiment	Marker?	Max mean expression
E-GEOD-93593	yes	550.45
E-MTAB-10287	yes	105.63
E-MTAB-8142	yes	97.73
E-HCAD-5	yes	40.36
E-HCAD-10	yes	35.31
E-GEOD-137537	yes	30.47
E-MTAB-7316	yes	28.05
E-CURD-112	yes	16.11
E-HCAD-11	yes	8.51
E-MTAB-6678	yes	4.12
E-GEOD-81383	no	725.84
E-MTAB-6108	no	419.47
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): CTNNB1

miRNA regulators (miRDB)

79 targeting RCN1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4713-3P	100.00	65.92	505
HSA-MIR-200B-3P	100.00	73.31	2693
HSA-MIR-200C-3P	100.00	73.35	2685
HSA-MIR-429	100.00	73.44	2698
HSA-MIR-4500	99.99	72.72	2367
HSA-LET-7A-5P	99.98	72.29	1790
HSA-LET-7B-5P	99.98	72.31	1790
HSA-LET-7C-5P	99.98	72.29	1790
HSA-LET-7E-5P	99.98	72.29	1790
HSA-LET-7F-5P	99.98	72.56	1784
HSA-LET-7G-5P	99.98	72.37	1784
HSA-LET-7I-5P	99.98	72.37	1788
HSA-MIR-98-5P	99.98	72.33	1787
HSA-LET-7D-5P	99.96	71.76	1632
HSA-MIR-4458	99.96	71.64	1650
HSA-MIR-495-3P	99.96	72.81	4197
HSA-MIR-5688	99.96	73.23	4504
HSA-MIR-23A-3P	99.95	74.24	3163
HSA-MIR-23B-3P	99.95	74.24	3163
HSA-MIR-23C	99.95	73.92	3192
HSA-MIR-3910	99.95	71.13	2227
HSA-MIR-335-3P	99.93	73.36	4958
HSA-MIR-5195-3P	99.92	70.92	1877
HSA-MIR-145-5P	99.92	71.13	1836
HSA-MIR-1297	99.91	73.41	3162
HSA-MIR-7162-3P	99.89	68.16	1682
HSA-MIR-12119	99.87	68.35	1653
HSA-MIR-202-3P	99.84	71.41	1290
HSA-MIR-3180-5P	99.82	69.12	2422
HSA-MIR-26A-5P	99.78	73.52	2303

Literature-anchored findings (GeneRIF, showing 17)

• RCN1 also is expressed on the cell surface of several endothelial cell lines, including human dermal microvascular endothelial cells (HDMVECs), bone marrow endothelial cells (BMEC), and transformed human bone marrow endothelial cells (TrHBMEC). (PMID:18561328)
• Data show that calumenin in the presence of calcium binds specifically to thrombospondin-1, but closely-related reticulocalbin does not form a similar complex. (PMID:18688696)
• deletion of Rcn1 directly or indirectly contributes to the eye phenotype in Pax6 contiguous gene deletions (PMID:19474196)
• Increased RCN1 is associated with systemic sclerosis and nephrogenic systemic fibrosis. (PMID:20724591)
• reticulocalbin-1 may be an important molecule in understanding lymphatic endothelial cells in tumors function and control of lymphatic metastasis. (PMID:21272564)
• a promising role of RCN1 as a possible marker in Renal cell carcinoma (PMID:23916412)
• Ca(2+) binding caused an increase in the alpha-helix content of human RCN1. On the other hand, RCN1 did not change the structure with Mg. (PMID:24451493)
• reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance (PMID:25242635)
• RCN1 expression was not statistically significantly different to healthy controls but was associated with disease activity score and could be used as a stratification biomarker for systemic sclerosis patients. (PMID:27468573)
• irradiated tumor cells were observed to significantly up-regulate the expression of calcium-binding proteins CALM1, CALU, and RCN1, suggesting important roles for these mediators in promoting tumor cell survival during hypoxia (PMID:27790916)
• RCN1 may promote cell survival and serve as a useful target for cancer therapy. (PMID:29453900)
• Our data indicate that RCN1 expression may have an vital role at promoting the occurrence of non-small cell lung cancer (PMID:30172915)
• Reticulocalbin-1 knockdown increases the sensitivity of cells to Adriamycin in nasopharyngeal carcinoma and promotes endoplasmic reticulum stress-induced cell apoptosis. (PMID:32436770)
• Salivary NUS1 and RCN1 Levels as Biomarkers for Oral Squamous Cell Carcinoma Diagnosis. (PMID:32871760)
• Downregulation of RCN1 promotes pyroptosis in acute myeloid leukemia cells. (PMID:37746742)
• RCN1 deficiency inhibits oral squamous cell carcinoma progression and THP-1 macrophage M2 polarization. (PMID:38057406)
• Downregulation of RCN1 inhibits esophageal squamous cell carcinoma progression and M2 macrophage polarization. (PMID:38713738)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	rcn1	ENSDARG00000070006
mus_musculus	Rcn1	ENSMUSG00000005973
rattus_norvegicus	Rcn1	ENSRNOG00000013452
drosophila_melanogaster	scf	FBGN0025682
caenorhabditis_elegans		WBGENE00019760

Paralogs (4): SDF4 (ENSG00000078808), RCN2 (ENSG00000117906), CALU (ENSG00000128595), RCN3 (ENSG00000142552)

Protein

Protein identifiers

Reticulocalbin-1 — Q15293 (reviewed: Q15293)

All UniProt accessions (5): E9PLM2, Q15293, H0YDA4, H0YER5, V9HW95

UniProt curated annotations — full annotation on UniProt →

Function. May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.

Subcellular location. Endoplasmic reticulum lumen.

Post-translational modifications. O-glycosylated. O-mannosylated by POMT1 and POMT2 and elongated by POMGNT1.

Miscellaneous. This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.

Similarity. Belongs to the CREC family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q15293-1	1	yes
Q15293-2	2

RefSeq proteins (1): NP_002892* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR002048	EF_hand_dom	Domain
IPR011992	EF-hand-dom_pair	Homologous_superfamily
IPR018247	EF_Hand_1_Ca_BS	Binding_site
IPR027241	Rcn1	Family

Pfam: PF13202, PF13499

UniProt features (44 total): binding site 28, domain 6, modified residue 3, sequence variant 2, signal peptide 1, chain 1, glycosylation site 1, splice variant 1, short sequence motif 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q15293-F1	76.76	0.47

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (28): 94; 96; 103; 128; 130; 132; 134; 139; 179; 181; 183; 185 …

Post-translational modifications (3): 55, 76, 80

Glycosylation sites (1): 53

Function

Pathways and Gene Ontology

Reactome pathways

2 pathways

ID	Pathway
R-HSA-381426	Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-HSA-8957275	Post-translational protein phosphorylation

MSigDB gene sets: 302 (showing top): GSE18804_SPLEEN_MACROPHAGE_VS_COLON_TUMORAL_MACROPHAGE_UP, GOBP_EMBRYO_DEVELOPMENT_ENDING_IN_BIRTH_OR_EGG_HATCHING, AAGCAAT_MIR137, YAATNRNNNYNATT_UNKNOWN, SOX9_B1, GOBP_IN_UTERO_EMBRYONIC_DEVELOPMENT, FOSTER_TOLERANT_MACROPHAGE_UP, ACEVEDO_LIVER_TUMOR_VS_NORMAL_ADJACENT_TISSUE_DN, COATES_MACROPHAGE_M1_VS_M2_UP, SCHLOSSER_MYC_TARGETS_AND_SERUM_RESPONSE_UP, DODD_NASOPHARYNGEAL_CARCINOMA_UP, NEMETH_INFLAMMATORY_RESPONSE_LPS_UP, PETROVA_ENDOTHELIUM_LYMPHATIC_VS_BLOOD_DN, BRUNO_HEMATOPOIESIS, JAATINEN_HEMATOPOIETIC_STEM_CELL_UP

GO Biological Process (2): in utero embryonic development (GO:0001701), camera-type eye development (GO:0043010)

GO Molecular Function (3): calcium ion binding (GO:0005509), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (2): endoplasmic reticulum (GO:0005783), endoplasmic reticulum lumen (GO:0005788)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
Metabolism of proteins	1
Post-translational protein modification	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
chordate embryonic development	1
eye development	1
metal ion binding	1
binding	1
cation binding	1
cytoplasm	1
endomembrane system	1
intracellular membrane-bounded organelle	1
endoplasmic reticulum	1
intracellular organelle lumen	1

Protein interactions and networks

STRING

868 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
RCN1	KDELR1	P24390	840
RCN1	PPP3CA	Q08209	588
RCN1	WT1	P19544	550
RCN1	FBXW7	Q969H0	545
RCN1	PAX6	P26367	541
RCN1	PDIA3	P30101	516
RCN1	ERP29	P30040	512
RCN1	ERP44	Q9BS26	489
RCN1	PDIA4	P13667	469
RCN1	RIT2	Q99578	468
RCN1	SEC63	Q9UGP8	462
RCN1	GOLIM4	O00461	461
RCN1	MST1R	Q04912	449
RCN1	HYOU1	Q9Y4L1	424
RCN1	RGN	Q15493	415

IntAct

344 interactions, top by confidence:

A	B	Type	Score
NRBP1	TSC22D2	psi-mi:“MI:0914”(association)	0.730
CFTR	XPO1	psi-mi:“MI:0914”(association)	0.710
ATXN1	RCN1	psi-mi:“MI:0915”(physical association)	0.670
DCAF11	RCN1	psi-mi:“MI:0915”(physical association)	0.560
GET3	RCN1	psi-mi:“MI:0915”(physical association)	0.560
SUOX	RCN1	psi-mi:“MI:0915”(physical association)	0.560
GTPBP3	RCN1	psi-mi:“MI:0915”(physical association)	0.560
LMO4	RCN1	psi-mi:“MI:0915”(physical association)	0.560
MYO15B	RCN1	psi-mi:“MI:0915”(physical association)	0.560
CETN3	RCN1	psi-mi:“MI:0915”(physical association)	0.560
RCN1	DCAF11	psi-mi:“MI:0915”(physical association)	0.560
RCN1	GTPBP3	psi-mi:“MI:0915”(physical association)	0.560
RCN1	ZNF410	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (359): RCN1 (Affinity Capture-MS), RCN1 (Affinity Capture-RNA), RCN1 (Affinity Capture-RNA), RCN1 (Affinity Capture-MS), RCN1 (Affinity Capture-MS), RCN1 (Affinity Capture-MS), RCN1 (Affinity Capture-MS), RCN1 (Affinity Capture-MS), RCN1 (Affinity Capture-MS), RCN1 (Affinity Capture-MS), RCN1 (Affinity Capture-MS), RCN1 (Affinity Capture-MS), RCN1 (Affinity Capture-MS), SPECC1L (Affinity Capture-MS), MYO18A (Affinity Capture-MS)

ESM2 similar proteins: A0A6I8RMG7, A0AVX7, A2VEI2, A4IG32, A5D7A0, B5X186, B5X4E0, I6L9G5, J3S9D9, O35783, O35887, O93390, O93434, P07214, P09486, P13213, P16975, P20112, P22676, P36377, P36378, P41044, P49257, P61022, P61023, P79881, Q05186, Q14257, Q15293, Q28BT4, Q2KJ39, Q3T0K1, Q4U471, Q5R767, Q5R7F0, Q5ZM44, Q62703, Q62902, Q6IP82, Q6IQP3

Diamond homologs: B5X186, B5X4E0, I6L9G5, J3S9D9, O35783, O35887, O43852, O93434, P02597, P04464, Q05186, Q14257, Q15293, Q28BT4, Q2KJ39, Q3T0K1, Q4U471, Q5RDD8, Q62703, Q66JA6, Q6IP82, Q6IQP3, Q6XLQ7, Q7SXV9, Q8BH97, Q8BP92, Q96D15, Q9FKW4, P42529, Q5ZKE5, Q03975, Q9M7R0

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

53 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	40
Likely benign	1
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

838 predictions. Top by Δscore:

Variant	Effect	Δscore
11:32091440:G:GT	donor_gain	1.0000
11:32091441:A:T	donor_gain	1.0000
11:32091445:GC:G	donor_gain	1.0000
11:32097283:G:GT	donor_gain	1.0000
11:32097301:G:GT	donor_gain	1.0000
11:32097301:G:T	donor_gain	1.0000
11:32097336:AGGTA:A	donor_loss	1.0000
11:32098515:A:T	donor_gain	1.0000
11:32098534:A:G	donor_gain	1.0000
11:32100604:TATTG:T	donor_gain	1.0000
11:32100606:TTGGT:T	donor_loss	1.0000
11:32100607:TGGTG:T	donor_loss	1.0000
11:32100608:GGT:G	donor_loss	1.0000
11:32100609:G:GG	donor_gain	1.0000
11:32100609:GTGA:G	donor_loss	1.0000
11:32100610:TGA:T	donor_loss	1.0000
11:32100611:G:GT	donor_loss	1.0000
11:32103279:A:AG	acceptor_gain	1.0000
11:32103279:AGC:A	acceptor_gain	1.0000
11:32103279:AGCG:A	acceptor_gain	1.0000
11:32103280:G:GT	acceptor_gain	1.0000
11:32103280:GC:G	acceptor_gain	1.0000
11:32103280:GCG:G	acceptor_gain	1.0000
11:32103280:GCGG:G	acceptor_gain	1.0000
11:32103280:GCGGA:G	acceptor_gain	1.0000
11:32103439:GC:G	donor_gain	1.0000
11:32103479:AGGT:A	donor_loss	1.0000
11:32103480:GG:G	donor_loss	1.0000
11:32103481:GT:G	donor_loss	1.0000
11:32103482:T:A	donor_loss	1.0000

AlphaMissense

2209 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
11:32091447:T:C	L84P	1.000
11:32097200:T:C	L104P	1.000
11:32097300:G:C	W137C	1.000
11:32097300:G:T	W137C	1.000
11:32098422:G:C	R174T	1.000
11:32098485:T:C	L195P	1.000
11:32098487:C:G	H196D	1.000
11:32100602:T:C	Y228H	1.000
11:32100603:A:G	Y228C	1.000
11:32103322:T:A	W244R	1.000
11:32103322:T:C	W244R	1.000
11:32103324:G:C	W244C	1.000
11:32103324:G:T	W244C	1.000
11:32103338:G:C	R249P	1.000
11:32103406:T:A	W272R	1.000
11:32103406:T:C	W272R	1.000
11:32103408:G:C	W272C	1.000
11:32103408:G:T	W272C	1.000
11:32103444:G:C	E284D	1.000
11:32103444:G:T	E284D	1.000
11:32103445:G:C	A285P	1.000
11:32103451:C:A	H287N	1.000
11:32103451:C:G	H287D	1.000
11:32103453:T:A	H287Q	1.000
11:32103453:T:G	H287Q	1.000
11:32103455:T:A	L288Q	1.000
11:32103455:T:C	L288P	1.000
11:32104375:T:C	L300P	1.000
11:32104390:T:A	I305K	1.000
11:32104410:T:C	F312L	1.000

dbSNP variants (sampled 300 via entrez): RS1000528504 (11:32097903 G>A), RS1000570390 (11:32105654 C>T), RS1000622550 (11:32104218 T>C), RS1001045520 (11:32091324 C>G,T), RS1001115492 (11:32101187 A>G), RS1001680501 (11:32096654 C>A,T), RS1001792618 (11:32103225 G>A,C,T), RS1001891796 (11:32105426 C>T), RS1002010288 (11:32093790 A>G), RS1002154523 (11:32106151 A>G), RS1002291011 (11:32091793 A>C), RS1002343684 (11:32094089 C>T), RS1002461331 (11:32090071 G>C,T), RS1002544883 (11:32104167 C>T), RS1002758379 (11:32091570 A>C,G)

Disease associations

OMIM: gene MIM:602735 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

8 associations (top):

Study	Trait	p-value
GCST001818_4	Metabolite levels (HVA/5-HIAA ratio)	4.000000e-06
GCST004245_1	Body mass index	2.000000e-06
GCST006041_27	Major depressive disorder	4.000000e-07
GCST006585_855	Blood protein levels	2.000000e-06
GCST007709_283	General factor of neuroticism	9.000000e-09
GCST010002_234	Refractive error	2.000000e-49
GCST010243_67	Apolipoprotein B levels	4.000000e-08
GCST90002379_88	Basophil count	2.000000e-10

EFO canonical traits (6, from GWAS)

EFO ID	Trait name
EFO:0005131	HVA measurement
EFO:0005132	5-HIAA measurement
EFO:0004340	body mass index
EFO:0007660	neuroticism measurement
EFO:0004615	apolipoprotein B measurement
EFO:0005090	basophil count

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6066498 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
6.67	Kd	215.3	nM	CHEMBL3752910
6.67	ED50	215.3	nM	CHEMBL3752910

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149924: Binding affinity to human RCN1 incubated for 45 mins by Kinobead based pull down assay	kd	0.2153	uM

CTD chemical–gene interactions

57 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	decreases expression, decreases methylation, increases expression	4
sodium arsenite	decreases expression, increases expression	2
(+)-JQ1 compound	decreases expression	2
Acetaminophen	increases expression	2
Smoke	decreases expression, increases abundance	2
Tobacco Smoke Pollution	increases expression, affects expression	2
aristolochic acid I	decreases expression	1
FR900359	increases phosphorylation	1
bisphenol F	increases expression	1
2,4,6-tribromophenol	decreases expression	1
triphenyl phosphate	affects expression	1
sodium arsenate	decreases expression	1
pyrogallol 1,3-dimethyl ether	decreases expression, affects cotreatment	1
decabromobiphenyl ether	increases expression	1
arsenite	affects binding, increases reaction	1
tetrabromobisphenol A	decreases expression	1
zinc chromate	increases abundance, increases expression	1
S-(1,2-dichlorovinyl)cysteine	affects response to substance, increases expression	1
di-n-butylphosphoric acid	affects expression	1
chromium hexavalent ion	increases abundance, increases expression	1
CGP 52608	affects binding, increases reaction	1
K 7174	increases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, increases expression	1
bisphenol B	increases expression	1
2,2’,4,4’-tetrabromodiphenyl ether	decreases expression	1
pentabrominated diphenyl ether 100	increases expression	1
hexabrominated diphenyl ether 153	increases expression	1
LDN 193189	affects cotreatment, increases expression	1
bisphenol AF	increases expression	1
Resveratrol	affects cotreatment, increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652966	Binding	Binding affinity to human RCN1 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

1 cell lines: 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B3FV	Abcam HEK293T RCN1 KO	Transformed cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.