RFTN1

Summary

RFTN1 (raftlin, lipid raft linker 1, HGNC:30278) is a protein-coding gene on chromosome 3p24.3, encoding Raftlin (Q14699). Involved in protein trafficking via association with clathrin and AP2 complex.

Enables double-stranded RNA binding activity. Involved in B cell receptor signaling pathway; membrane raft assembly; and positive regulation of growth rate. Acts upstream of or within dsRNA transport; response to exogenous dsRNA; and toll-like receptor 3 signaling pathway. Located in endosome; membrane raft; and plasma membrane. Part of protein-containing complex. Biomarker of glaucoma.

Source: NCBI Gene 23180 — RefSeq curated summary.

At a glance

• GWAS associations: 6
• Clinical variants (ClinVar): 57 total
• Druggable target: yes
• MANE Select transcript: NM_015150

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:30278
Approved symbol	RFTN1
Name	raftlin, lipid raft linker 1
Location	3p24.3
Locus type	gene with protein product
Status	Approved
Aliases	MIG2, KIAA0084, FLJ23866, Raftlin
Ensembl gene	ENSG00000131378
Ensembl biotype	protein_coding
OMIM	618210
Entrez	23180

Gene structure

Transcript identifiers

Ensembl transcripts: 17 — 11 protein_coding, 4 protein_coding_CDS_not_defined, 2 nonsense_mediated_decay

ENST00000334133, ENST00000431547, ENST00000432519, ENST00000441460, ENST00000449415, ENST00000451036, ENST00000453536, ENST00000470458, ENST00000483671, ENST00000484752, ENST00000495666, ENST00000607320, ENST00000856941, ENST00000856943, ENST00000856944, ENST00000856946, ENST00000856947

RefSeq mRNA: 1 — MANE Select: NM_015150 NM_015150

CCDS: CCDS33712

Canonical transcript exons

ENST00000334133 — 10 exons

Exon	Start	End
ENSE00000901388	16377718	16378102
ENSE00000901391	16493725	16493877
ENSE00000901392	16513442	16513699
ENSE00001777050	16315845	16317232
ENSE00003491818	16357932	16358047
ENSE00003549196	16370076	16370279
ENSE00003555641	16433851	16434037
ENSE00003600304	16409375	16409483
ENSE00003641354	16326773	16326876
ENSE00003689062	16323376	16323457

Expression profiles

Bgee: expression breadth ubiquitous, 270 present calls, max score 97.83.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 24.6728 / max 394.7846, expressed in 1624 samples.

FANTOM5 promoters (11 alternative TSS)

Promoter ID	TPM avg	Samples expressed
41344	11.8620	1534
41345	5.7507	1233
41341	3.1134	786
41343	1.4203	811
41339	0.8695	421
41340	0.7527	262
41342	0.4625	243
41333	0.1941	104
41346	0.1213	58
41328	0.0942	43

Top tissues by expression

284 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
decidua	UBERON:0002450	97.83	gold quality
lymph node	UBERON:0000029	97.24	gold quality
saphenous vein	UBERON:0007318	97.11	gold quality
thymus	UBERON:0002370	96.15	gold quality
lower lobe of lung	UBERON:0008949	96.13	gold quality
superficial temporal artery	UBERON:0001614	95.56	gold quality
vermiform appendix	UBERON:0001154	95.10	gold quality
caecum	UBERON:0001153	95.07	gold quality
pericardium	UBERON:0002407	95.06	gold quality
right lobe of thyroid gland	UBERON:0001119	94.98	gold quality
thyroid gland	UBERON:0002046	94.95	gold quality
left lobe of thyroid gland	UBERON:0001120	94.92	gold quality
middle temporal gyrus	UBERON:0002771	94.82	gold quality
adipose tissue	UBERON:0001013	94.78	gold quality
apex of heart	UBERON:0002098	94.46	gold quality
lung	UBERON:0002048	94.45	gold quality
subcutaneous adipose tissue	UBERON:0002190	94.44	gold quality
vena cava	UBERON:0004087	94.44	gold quality
connective tissue	UBERON:0002384	94.41	gold quality
ascending aorta	UBERON:0001496	94.36	gold quality
thoracic aorta	UBERON:0001515	94.10	gold quality
right lung	UBERON:0002167	93.93	gold quality
sural nerve	UBERON:0015488	93.84	gold quality
urethra	UBERON:0000057	93.64	gold quality
tibial nerve	UBERON:0001323	93.59	gold quality
skin of hip	UBERON:0001554	93.59	gold quality
upper lobe of lung	UBERON:0008948	93.57	gold quality
adipose tissue of abdominal region	UBERON:0007808	93.44	gold quality
upper lobe of left lung	UBERON:0008952	93.36	gold quality
tonsil	UBERON:0002372	93.25	gold quality

Single-cell (SCXA)

Detected in 7 experiment(s), a significant marker in 4.

Experiment	Marker?	Max mean expression
E-GEOD-100618	yes	479.14
E-CURD-119	yes	16.88
E-ANND-3	yes	12.56
E-MTAB-8498	yes	10.07
E-MTAB-6075	no	1283.15
E-MTAB-7381	no	980.85
E-ENAD-17	no	60.25

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

59 targeting RFTN1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4789-5P	99.98	70.76	2721
HSA-MIR-19A-3P	99.98	75.33	2762
HSA-MIR-19B-3P	99.98	75.44	2754
HSA-MIR-1250-3P	99.96	70.04	4038
HSA-MIR-96-5P	99.95	72.80	2140
HSA-MIR-12133	99.92	71.82	2006
HSA-MIR-1271-5P	99.91	71.99	1972
HSA-MIR-153-5P	99.89	73.86	6317
HSA-MIR-182-5P	99.87	74.03	2589
HSA-MIR-137-3P	99.87	74.74	2401
HSA-MIR-4728-5P	99.85	69.39	4718
HSA-MIR-544A	99.84	68.66	1965
HSA-MIR-765	99.84	68.24	2442
HSA-MIR-3180-5P	99.82	69.12	2422
HSA-MIR-6844	99.82	70.69	2423
HSA-MIR-6785-5P	99.82	68.68	4428
HSA-MIR-548AJ-5P	99.78	71.12	3085
HSA-MIR-548G-5P	99.78	71.12	3085
HSA-MIR-548X-5P	99.78	71.12	3085
HSA-MIR-548F-5P	99.78	71.02	3093
HSA-MIR-8084	99.73	69.57	1760
HSA-MIR-6892-3P	99.68	66.40	1178
HSA-MIR-6715B-5P	99.64	69.63	1420
HSA-MIR-298	99.63	67.56	1916
HSA-MIR-6757-3P	99.63	66.88	1089
HSA-MIR-4269	99.55	69.89	1373
HSA-MIR-4643	99.49	67.63	1791
HSA-MIR-6722-3P	99.45	67.62	1919
HSA-MIR-330-3P	99.41	69.95	2521
HSA-MIR-542-3P	99.34	67.58	1270

Literature-anchored findings (GeneRIF, showing 11)

• These data suggest that Raftlin plays a pivotal role in the formation and/or maintenance of lipid rafts, therefore regulating B-cell antigen receptor-mediated signaling. (PMID:12805216)
• Raftlin cooperates with the uptake receptor to mediate cell entry of poly(I:C), which is critical for activation of TLR3. (PMID:21266579)
• combination of ATOH7 and RFTN1 SNPs increased risk to POAG, indicating their diversified effects in the complex genetics of glaucoma. (PMID:22222511)
• Mitogen-inducible Gene-2 expression is reduced in breast cancer. (PMID:23645746)
• this study shows that raftlin appears to modulate cargo selection as an accessary protein of clathrin-associated adaptor protein-2 in clathrin-mediated endocytosis of TLR3/4 ligands (PMID:27022195)
• Raftlin is recruited by neuropilin-1 to the activated VEGFR2 complex to control proangiogenic signaling. (PMID:32274611)
• RFTN1 facilitates gastric cancer progression by modulating AKT/p38 signaling pathways. (PMID:35490655)
• Expression of Major Lipid Raft Protein Raftlin in Chronic Rhinosinusitis with Nasal Polyps in Smoking and Non-Smoking Patients Correlated with Interleukin-17 and Tumor Necrosis Factor-alpha Levels. (PMID:36139155)
• Serum NOX1 and Raftlin as new potential biomarkers of Major Depressive Disorder: A study in treatment-naive first episode patients. (PMID:36341844)
• Raftlin and 8-iso-prostaglandin F2alpha levels and gene network analysis in patients with Modic changes. (PMID:37208489)
• Association between raftlin and presepsin levels with periodontal healthy and disease conditions. (PMID:38278125)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	rftn1a	ENSDARG00000070831
danio_rerio		ENSDARG00000098654
mus_musculus	Rftn1	ENSMUSG00000039316
rattus_norvegicus	Rftn1	ENSRNOG00000011321

Paralogs (1): RFTN2 (ENSG00000162944)

Protein

Protein identifiers

Raftlin — Q14699 (reviewed: Q14699)

Alternative names: Cell migration-inducing gene 2 protein, Raft-linking protein

All UniProt accessions (7): C9JHG2, C9JRN3, C9JWQ9, Q14699, F8WAR1, G3XAJ6, U3KPZ2

UniProt curated annotations — full annotation on UniProt →

Function. Involved in protein trafficking via association with clathrin and AP2 complex. Upon bacterial lipopolysaccharide stimulation, mediates internalization of TLR4 to endosomes in dendritic cells and macrophages; and internalization of poly(I:C) to TLR3-positive endosomes in myeloid dendritic cells and epithelial cells; resulting in activation of TICAM1-mediated signaling and subsequent IFNB1 production. Involved in T-cell antigen receptor-mediated signaling by regulating tyrosine kinase LCK localization, T-cell dependent antibody production and cytokine secretion. May regulate B-cell antigen receptor-mediated signaling. May play a pivotal role in the formation and/or maintenance of lipid rafts.

Subunit / interactions. Interacts with TLR4; the interaction occurs in response to lipopolysaccharide stimulation. Interacts with CLTC; the interaction occurs in response to pathogens. Interacts with AP2A1 and AP2B1.

Subcellular location. Cell membrane. Cytoplasm. Membrane raft. Endosome. Early endosome.

Tissue specificity. Expressed in B-cells (at protein level). Expressed in dendritic cells and macrophages.

Similarity. Belongs to the raftlin family.

RefSeq proteins (1): NP_055965* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR028169	Raftlin	Family

Pfam: PF15250

UniProt features (24 total): compositionally biased region 6, modified residue 4, region of interest 3, sequence conflict 3, lipid moiety-binding region 2, sequence variant 2, mutagenesis site 2, initiator methionine 1, chain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q14699-F1	65.22	0.33

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (6): 183, 199, 220, 505, 2, 3

Mutagenesis-validated functional residues (2):

Position	Phenotype
2	loss of association with membranes. same effect; when associated with s-3.
3	partially affects association with membranes. loss of association with membranes; when associated with a-2.

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 319 (showing top): GOBP_RESPONSE_TO_NITROGEN_COMPOUND, GOBP_INTRACELLULAR_PROTEIN_TRANSPORT, GOBP_MEMBRANE_BIOGENESIS, GOZGIT_ESR1_TARGETS_DN, GOBP_ANTIGEN_RECEPTOR_MEDIATED_SIGNALING_PATHWAY, GOBP_GROWTH, HSIAO_HOUSEKEEPING_GENES, GOBP_POSITIVE_REGULATION_OF_CYTOKINE_PRODUCTION, GOBP_MEMBRANE_RAFT_ORGANIZATION, GOBP_LEUKOCYTE_MEDIATED_IMMUNITY, GOBP_POSITIVE_REGULATION_OF_RESPONSE_TO_EXTERNAL_STIMULUS, GOBP_REGULATION_OF_IMMUNE_RESPONSE, GOBP_DEFENSE_RESPONSE_TO_OTHER_ORGANISM, RUTELLA_RESPONSE_TO_CSF2RB_AND_IL4_UP, GOBP_LYMPHOCYTE_MEDIATED_IMMUNITY

GO Biological Process (11): membrane raft assembly (GO:0001765), T cell antigen processing and presentation (GO:0002457), protein transport into membrane raft (GO:0032596), positive regulation of interleukin-17 production (GO:0032740), dsRNA transport (GO:0033227), toll-like receptor 3 signaling pathway (GO:0034138), positive regulation of growth rate (GO:0040010), response to exogenous dsRNA (GO:0043330), T cell receptor signaling pathway (GO:0050852), B cell receptor signaling pathway (GO:0050853), protein localization to membrane raft (GO:1903044)

GO Molecular Function (1): double-stranded RNA binding (GO:0003725)

GO Cellular Component (8): cytoplasm (GO:0005737), endosome (GO:0005768), early endosome (GO:0005769), plasma membrane (GO:0005886), protein-containing complex (GO:0032991), membrane raft (GO:0045121), extracellular exosome (GO:0070062), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
antigen receptor-mediated signaling pathway	2
cellular anatomical structure	2
membrane raft organization	1
membrane assembly	1
T cell mediated immunity	1
antigen processing and presentation	1
protein transport within lipid bilayer	1
establishment of protein localization to membrane	1
protein localization to membrane raft	1
positive regulation of cytokine production	1
interleukin-17 production	1
regulation of interleukin-17 production	1
RNA transport	1
endolysosomal toll-like receptor signaling pathway	1
regulation of growth rate	1
positive regulation of growth	1
response to dsRNA	1
protein localization to membrane	1
RNA binding	1
intracellular anatomical structure	1
endomembrane system	1
cytoplasmic vesicle	1
endosome	1
membrane	1
cell periphery	1
cellular_component	1
membrane microdomain	1
extracellular vesicle	1

Protein interactions and networks

STRING

530 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
RFTN1	OR4K17	Q8NGC6	469
RFTN1	OXNAD1	Q96HP4	451
RFTN1	DAZL	Q92904	417
RFTN1	CZIB	Q9NWV4	385
RFTN1	GALNT15	Q8N3T1	378
RFTN1	USHBP1	Q8N6Y0	376
RFTN1	ZCCHC7	Q8N3Z6	373
RFTN1	DPH3	Q96FX2	371
RFTN1	NDNF	Q8TB73	360
RFTN1	LAMA4	Q16363	359
RFTN1	KIAA1191	Q96A73	357
RFTN1	ATG2A	Q2TAZ0	349
RFTN1	GALNTL5	Q7Z4T8	345
RFTN1	ROBO2	Q9HCK4	314
RFTN1	RCCD1	A6NED2	307

IntAct

53 interactions, top by confidence:

A	B	Type	Score
UNC119	UNC119B	psi-mi:“MI:0914”(association)	0.640
SLC17A5	LGALS8	psi-mi:“MI:0914”(association)	0.640
GJB7	PALM3	psi-mi:“MI:0914”(association)	0.530
SLC25A41	NUDT19	psi-mi:“MI:0914”(association)	0.530
SYNGAP1	IGF2BP3	psi-mi:“MI:0914”(association)	0.530
SYNGAP1	SEC16A	psi-mi:“MI:0914”(association)	0.530
TGM5	UBB	psi-mi:“MI:0914”(association)	0.530
UNC119	PDE8A	psi-mi:“MI:0914”(association)	0.530
DOK4	KDM1A	psi-mi:“MI:0914”(association)	0.530
GPIHBP1	ADAM10	psi-mi:“MI:0914”(association)	0.530
LPAR1	TMEM223	psi-mi:“MI:0914”(association)	0.530
SDC1	ILVBL	psi-mi:“MI:0915”(physical association)	0.400
		psi-mi:“MI:0914”(association)	0.350
	VWA8	psi-mi:“MI:0914”(association)	0.350
	ESYT2	psi-mi:“MI:0914”(association)	0.350
E5	ESYT2	psi-mi:“MI:0914”(association)	0.350
	PGRMC1	psi-mi:“MI:0914”(association)	0.350
	SNAP23	psi-mi:“MI:0914”(association)	0.350
	HAX1	psi-mi:“MI:0914”(association)	0.350
BVLF1	VWA8	psi-mi:“MI:0914”(association)	0.350
	KPNA4	psi-mi:“MI:0914”(association)	0.350
ESR1	ESYT2	psi-mi:“MI:0914”(association)	0.350

BioGRID (65): RFTN1 (Affinity Capture-MS), RFTN1 (Affinity Capture-MS), RFTN1 (Synthetic Growth Defect), RFTN1 (Synthetic Growth Defect), RFTN1 (Proximity Label-MS), RFTN1 (Affinity Capture-MS), RABGAP1L (Affinity Capture-MS), RFTN1 (Affinity Capture-MS), ATG2A (Affinity Capture-MS), RFTN1 (Affinity Capture-MS), RFTN1 (Affinity Capture-MS), RFTN1 (Affinity Capture-MS), RFTN1 (Affinity Capture-MS), RFTN1 (Proximity Label-MS), RFTN1 (Affinity Capture-MS)

ESM2 similar proteins: A0A0M3U1B0, A0A1L8EYB2, A0JMF7, A4D1B5, A4FUB0, D3IUT5, D3Z6S9, F1QB81, O60281, O70167, P53995, Q0VCQ7, Q13129, Q14699, Q2T9I9, Q3TCV3, Q3UJC8, Q402B2, Q4R9E9, Q5RA75, Q5RB52, Q5XI46, Q5XI56, Q5ZKI7, Q659A1, Q6AYM1, Q6DRL4, Q6INI0, Q6PUR7, Q7Z2Z1, Q8BQ33, Q8CCC3, Q8CDN1, Q8IXR9, Q8K1K4, Q8NB91, Q8ND61, Q90WN7, Q920I9, Q92674

Diamond homologs: Q14699, Q52LD8, Q5R458, Q6A0D4, Q7SZI5, Q8CHX7

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

57 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	46
Likely benign	5
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

2821 predictions. Top by Δscore:

Variant	Effect	Δscore
3:16317233:C:CC	acceptor_gain	1.0000
3:16357925:CACT:C	donor_loss	1.0000
3:16357926:ACTTA:A	donor_loss	1.0000
3:16357927:CT:C	donor_loss	1.0000
3:16357928:TTACT:T	donor_loss	1.0000
3:16357929:T:TG	donor_loss	1.0000
3:16357930:A:AC	donor_gain	1.0000
3:16357930:A:T	donor_loss	1.0000
3:16357930:ACTT:A	donor_gain	1.0000
3:16357931:C:CG	donor_gain	1.0000
3:16357931:CTT:C	donor_gain	1.0000
3:16357931:CTTC:C	donor_gain	1.0000
3:16357933:T:TA	donor_gain	1.0000
3:16357970:AGC:A	donor_gain	1.0000
3:16358045:AATC:A	acceptor_loss	1.0000
3:16358046:ATCT:A	acceptor_loss	1.0000
3:16358048:C:CC	acceptor_gain	1.0000
3:16358048:C:CG	acceptor_loss	1.0000
3:16370070:ACAT:A	donor_loss	1.0000
3:16370071:CATA:C	donor_loss	1.0000
3:16370072:ATAC:A	donor_loss	1.0000
3:16370073:TACCA:T	donor_loss	1.0000
3:16370074:A:AC	donor_gain	1.0000
3:16370075:C:CC	donor_gain	1.0000
3:16370075:C:CT	donor_loss	1.0000
3:16370075:CCAT:C	donor_gain	1.0000
3:16370075:CCATT:C	donor_gain	1.0000
3:16370290:T:C	acceptor_gain	1.0000
3:16370290:T:TC	acceptor_gain	1.0000
3:16370292:G:C	acceptor_gain	1.0000

AlphaMissense

3779 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
3:16326819:A:G	W402R	1.000
3:16326819:A:T	W402R	1.000
3:16323422:A:G	L429P	0.999
3:16323428:A:T	V427D	0.999
3:16326817:C:A	W402C	0.999
3:16326817:C:G	W402C	0.999
3:16357954:A:T	V375D	0.999
3:16323415:T:A	R431S	0.998
3:16323415:T:G	R431S	0.998
3:16323416:C:G	R431T	0.998
3:16326818:C:G	W402S	0.998
3:16326833:A:G	L397P	0.998
3:16357960:A:T	I373N	0.998
3:16370154:A:G	W318R	0.998
3:16370154:A:T	W318R	0.998
3:16326821:C:A	G401V	0.997
3:16326842:A:G	L394P	0.997
3:16326845:A:G	L393P	0.997
3:16493795:C:A	R25S	0.997
3:16493795:C:G	R25S	0.997
3:16323425:A:G	F428S	0.996
3:16326821:C:T	G401D	0.996
3:16326833:A:T	L397Q	0.996
3:16357957:A:T	V374E	0.996
3:16357960:A:C	I373S	0.996
3:16493809:A:G	S21P	0.996
3:16357964:C:G	A372P	0.995
3:16493796:C:G	R25T	0.995
3:16493802:A:G	L23S	0.995
3:16493814:A:G	I19T	0.995

dbSNP variants (sampled 300 via entrez): RS1000001162 (3:16511162 G>T), RS1000008174 (3:16474307 C>A), RS1000009079 (3:16513952 C>G,T), RS1000037621 (3:16504573 G>A), RS1000039465 (3:16348614 C>A,T), RS1000042990 (3:16339118 G>A), RS1000076333 (3:16468320 C>T), RS1000103320 (3:16405381 T>C), RS1000115835 (3:16338875 C>T), RS1000116478 (3:16480342 G>A,T), RS1000127596 (3:16487157 G>A), RS1000128389 (3:16445039 A>G), RS1000157161 (3:16428012 T>C), RS1000166798 (3:16477134 T>C), RS1000185404 (3:16432684 A>AG)

Disease associations

OMIM: gene MIM:618210 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

6 associations (top):

Study	Trait	p-value
GCST000426_10	Obesity (extreme)	5.000000e-06
GCST000655_1	Optic nerve measurement (cup-to-disc ratio)	6.000000e-07
GCST000659_3	Optic nerve measurement (cup area)	2.000000e-07
GCST002826_9	Urate levels (BMI interaction)	6.000000e-06
GCST003542_179	Night sleep phenotypes	2.000000e-06
GCST009189_4	Lateral orbital frontal cortex volume	4.000000e-06

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0004340	body mass index
EFO:0004531	urate measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6066455 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.20	Kd	6329	nM	CHEMBL5653589
5.20	ED50	6329	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149223: Binding affinity to human RFTN1 incubated for 45 mins by Kinobead based pull down assay	kd	6.3286	uM

CTD chemical–gene interactions

79 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	affects cotreatment, increases expression, affects expression, decreases expression, decreases methylation	8
bisphenol A	decreases expression, decreases methylation, increases expression	3
Tetrachlorodibenzodioxin	affects cotreatment, increases expression, affects binding, increases reaction, decreases expression	3
Tobacco Smoke Pollution	affects expression, decreases expression	3
Tretinoin	increases expression, decreases expression	3
sodium arsenite	increases expression, increases abundance	2
(+)-JQ1 compound	decreases expression	2
Arsenic	increases abundance, increases expression	2
Cisplatin	affects cotreatment, decreases expression	2
Estradiol	affects cotreatment, increases expression	2
Tamoxifen	affects expression, affects cotreatment, decreases expression	2
Aflatoxin B1	decreases expression, decreases methylation	2
Particulate Matter	decreases expression, increases abundance, affects cotreatment	2
FR900359	decreases phosphorylation	1
bisphenol F	increases expression	1
2,4,6-tribromophenol	increases expression	1
methylmercuric chloride	increases expression	1
methyleugenol	decreases expression	1
triphenyl phosphate	affects expression	1
sodium arsenate	increases abundance, increases expression	1
terbufos	increases methylation	1
trichostatin A	decreases expression, increases expression	1
tris(1,3-dichloro-2-propyl)phosphate	decreases expression	1
cobaltous chloride	decreases expression	1
tetrabromobisphenol A	increases expression	1
tobacco tar	decreases expression	1
benzo(e)pyrene	increases methylation	1
2,3-bis(3’-hydroxybenzyl)butyrolactone	decreases expression, affects cotreatment	1
cupric chloride	increases expression	1
hydroquinone	increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652265	Binding	Binding affinity to human RFTN1 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.