RNF141

Summary

RNF141 (ring finger protein 141, HGNC:21159) is a protein-coding gene on chromosome 11p15.3, encoding RING finger protein 141 (Q8WVD5). May be involved in spermatogenesis.

The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-DNA and protein-protein interactions. Abundant expression of this gene was found in the testicular tissue of fertile men, but was not detected in azoospermic patients. Studies of the mouse counterpart suggest that this gene may function as a testis specific transcription factor during spermatogenesis.

Source: NCBI Gene 50862 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 21 total
• MANE Select transcript: NM_016422

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:21159
Approved symbol	RNF141
Name	ring finger protein 141
Location	11p15.3
Locus type	gene with protein product
Status	Approved
Aliases	ZFP26, ZNF230
Ensembl gene	ENSG00000110315
Ensembl biotype	protein_coding
OMIM	616641
Entrez	50862

Gene structure

Transcript identifiers

Ensembl transcripts: 11 — 9 protein_coding, 1 nonsense_mediated_decay, 1 retained_intron

ENST00000265981, ENST00000528665, ENST00000530156, ENST00000533412, ENST00000534281, ENST00000861250, ENST00000861251, ENST00000861252, ENST00000930647, ENST00000967208, ENST00000967209

RefSeq mRNA: 1 — MANE Select: NM_016422 NM_016422

CCDS: CCDS7803

Canonical transcript exons

ENST00000265981 — 6 exons

Exon	Start	End
ENSE00000889988	10511673	10515066
ENSE00000911009	10534016	10534205
ENSE00002171319	10541122	10541187
ENSE00003459264	10525192	10525373
ENSE00003618451	10530643	10530751
ENSE00003785771	10519034	10519141

Expression profiles

Bgee: expression breadth ubiquitous, 273 present calls, max score 98.26.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 32.0816 / max 412.3187, expressed in 1803 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
118610	31.6148	1803
206187	0.4667	212

Top tissues by expression

291 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
endothelial cell	CL:0000115	98.26	gold quality
upper leg skin	UBERON:0004262	97.33	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	97.33	gold quality
corpus callosum	UBERON:0002336	97.17	gold quality
skin of hip	UBERON:0001554	97.07	gold quality
subthalamic nucleus	UBERON:0001906	97.06	gold quality
inferior vagus X ganglion	UBERON:0005363	96.81	gold quality
spinal cord	UBERON:0002240	96.80	gold quality
left testis	UBERON:0004533	96.79	gold quality
oral cavity	UBERON:0000167	96.71	gold quality
right testis	UBERON:0004534	96.41	gold quality
lateral globus pallidus	UBERON:0002476	96.30	gold quality
sperm	CL:0000019	96.25	gold quality
substantia nigra pars reticulata	UBERON:0001966	95.91	gold quality
globus pallidus	UBERON:0001875	95.64	gold quality
esophagus squamous epithelium	UBERON:0006920	95.52	gold quality
substantia nigra pars compacta	UBERON:0001965	95.46	gold quality
superior vestibular nucleus	UBERON:0007227	95.33	gold quality
medial globus pallidus	UBERON:0002477	95.31	gold quality
pons	UBERON:0000988	95.18	gold quality
parotid gland	UBERON:0001831	95.12	gold quality
gingiva	UBERON:0001828	95.10	gold quality
postcentral gyrus	UBERON:0002581	94.86	gold quality
testis	UBERON:0000473	94.79	gold quality
epithelium of esophagus	UBERON:0001976	94.69	gold quality
gingival epithelium	UBERON:0001949	94.40	gold quality
monocyte	CL:0000576	94.32	gold quality
biceps brachii	UBERON:0001507	94.27	gold quality
parietal lobe	UBERON:0001872	94.04	gold quality
male germ cell	CL:0000015	93.95	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: yes

Downstream targets (CollecTRI)

2 targets.

Target	Regulation
CDKN1C
ZFP36

miRNA regulators (miRDB)

156 targeting RNF141, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-5692A	100.00	74.40	6850
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-4668-3P	100.00	68.74	2635
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-513A-5P	100.00	69.77	2465
HSA-MIR-3924	100.00	72.09	2394
HSA-MIR-656-3P	100.00	72.15	2788
HSA-MIR-3646	100.00	73.56	5283
HSA-MIR-4481	100.00	66.42	1669
HSA-MIR-6077	99.99	68.04	2299
HSA-MIR-150-5P	99.99	66.69	1976
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-548P	99.98	72.25	3784
HSA-MIR-27A-3P	99.98	72.13	2955
HSA-MIR-27B-3P	99.98	72.13	2955
HSA-MIR-9985	99.98	72.11	2939
HSA-MIR-32-5P	99.98	75.21	1964
HSA-MIR-92A-3P	99.98	75.21	1960
HSA-MIR-92B-3P	99.98	75.25	1955
HSA-MIR-25-3P	99.98	74.60	1817
HSA-MIR-363-3P	99.98	74.72	1821
HSA-MIR-367-3P	99.98	74.83	1819
HSA-LET-7F-2-3P	99.98	70.98	2588
HSA-MIR-1185-1-3P	99.98	71.04	2593
HSA-MIR-1185-2-3P	99.98	71.04	2593
HSA-MIR-4775	99.98	75.00	6394
HSA-MIR-548AT-5P	99.96	70.83	2666
HSA-MIR-590-3P	99.96	74.34	6478

Literature-anchored findings (GeneRIF, showing 2)

• Analysis of the mouse counterpart. (PMID:12804569)
• A germ cell-specific transcription factor RFP141 reveals a unique RING fold. (PMID:28547869)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	rnf141	ENSDARG00000041802
mus_musculus	Rnf141	ENSMUSG00000030788
rattus_norvegicus		ENSRNOG00000079384
caenorhabditis_elegans		WBGENE00010028

Protein

Protein identifiers

RING finger protein 141 — Q8WVD5 (reviewed: Q8WVD5)

Alternative names: Zinc finger protein 230

All UniProt accessions (4): Q8WVD5, E9PLX2, E9PNF8, E9PQV2

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in spermatogenesis.

Subcellular location. Membrane.

Tissue specificity. Isoform 1 is testis-specific. Isoform 2 is expressed in heart, brain, skeletal muscle, kidney and pancreas. Isoform 1 is not expressed in fetus or in azoospermic patients.

Isoforms (2)

UniProt ID	Names	Canonical?
Q8WVD5-1	1, Short	yes
Q8WVD5-2	2, Long

RefSeq proteins (1): NP_057506* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001841	Znf_RING	Domain
IPR013083	Znf_RING/FYVE/PHD	Homologous_superfamily
IPR017907	Znf_RING_CS	Conserved_site
IPR043400	RING-HC_RNF141	Domain
IPR047126	RNF141-like	Family

Pfam: PF13639

UniProt features (11 total): strand 3, helix 2, initiator methionine 1, chain 1, zinc finger region 1, lipid moiety-binding region 1, sequence conflict 1, turn 1

Structure

Experimental structures (PDB)

2 structures.

PDB	Method	Resolution (Å)
2ECN	SOLUTION NMR
5XEK	SOLUTION NMR

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8WVD5-F1	83.83	0.51

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (1): 2

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 232 (showing top): GCM_MAP4K4, BROWNE_HCMV_INFECTION_6HR_DN, STAEGE_EWING_FAMILY_TUMOR, CHIANG_LIVER_CANCER_SUBCLASS_UNANNOTATED_DN, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_DN, IVANOVA_HEMATOPOIESIS_MATURE_CELL, EFC_Q6, GTGCCTT_MIR506, GOBP_PROTEIN_AUTOUBIQUITINATION, GOBP_POST_TRANSLATIONAL_PROTEIN_MODIFICATION, DOUGLAS_BMI1_TARGETS_DN, JOSEPH_RESPONSE_TO_SODIUM_BUTYRATE_DN, GCM_MAPK10, DANG_BOUND_BY_MYC, RIGGI_EWING_SARCOMA_PROGENITOR_UP

GO Biological Process (2): regulation of DNA-templated transcription (GO:0006355), protein autoubiquitination (GO:0051865)

GO Molecular Function (4): ubiquitin-protein transferase activity (GO:0004842), zinc ion binding (GO:0008270), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (1): membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
DNA-templated transcription	1
regulation of gene expression	1
regulation of RNA biosynthetic process	1
protein ubiquitination	1
ubiquitin-like protein transferase activity	1
transition metal ion binding	1
binding	1
cation binding	1
cellular anatomical structure	1

Protein interactions and networks

STRING

502 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
RNF141	LRRC18	Q8N456	549
RNF141	SPATA4	Q8NEY3	447
RNF141	DYNLT2	Q8IZS6	445
RNF141	ZNF219	Q9P2Y4	434
RNF141	RNF208	Q9H0X6	429
RNF141	CCDC42	Q96M95	420
RNF141	STOM	P27105	418
RNF141	RSPH1	Q8WYR4	402
RNF141	GOLGA3	Q08378	395
RNF141	TSSK6	Q9BXA6	381
RNF141	OSTF1	Q92882	375
RNF141	CAPZA3	Q96KX2	375
RNF141	RNF113B	Q8IZP6	372
RNF141	FAM171A2	A8MVW0	371
RNF141	RNF181	Q9P0P0	368

IntAct

12 interactions, top by confidence:

A	B	Type	Score
RNF141	PLK3	psi-mi:“MI:0915”(physical association)	0.560
MVK	RNF141	psi-mi:“MI:0915”(physical association)	0.560
TRIM24	RNF141	psi-mi:“MI:0915”(physical association)	0.370
RNF141	TRIM8	psi-mi:“MI:0915”(physical association)	0.370
RNF141	DTX3	psi-mi:“MI:0915”(physical association)	0.370
RNF141	CST4	psi-mi:“MI:0914”(association)	0.350
PLK3	RNF141	psi-mi:“MI:0915”(physical association)	0.000
MVK	RNF141	psi-mi:“MI:0915”(physical association)	0.000
Map3k1	UBA1	psi-mi:“MI:0220”(ubiquitination reaction)	0.000

BioGRID (15): RNF141 (Biochemical Activity), MVK (Two-hybrid), PLK3 (Two-hybrid), IGHA1 (Affinity Capture-MS), CST1 (Affinity Capture-MS), CST4 (Affinity Capture-MS), ZG16B (Affinity Capture-MS), KRAS (Affinity Capture-Western), RNF141 (Affinity Capture-Western), KRAS (PCA), RNF141 (Reconstituted Complex), RNF141 (Affinity Capture-RNA), DTX3 (Two-hybrid), RNF141 (Two-hybrid), TRIM8 (Two-hybrid)

ESM2 similar proteins: A0PJ23, A9JR22, A9JR44, B0BLK0, B0BLK7, B0BLK9, B2C4J2, B2MW50, B2ZDY1, B6VQ60, G5EDM7, J7H5K9, O43034, O73557, P05075, P0DKL9, P18541, P19325, P27426, Q03607, Q06651, Q11107, Q17768, Q197D3, Q20082, Q27YE2, Q27YE6, Q2XNS1, Q3UKZ7, Q5R7K8, Q5UPV2, Q6BET5, Q6IUF9, Q6IV57, Q6IVU5, Q6R7D2, Q6R7D3, Q6RSS3, Q6UY62, Q6UY71

Diamond homologs: A0A1L8FM16, A6NGJ6, A6NI03, A6NLI5, G2Q0E2, O74747, O76064, P19474, P25916, P35226, Q02398, Q28H21, Q2HJ46, Q2XNS1, Q32KX7, Q32L15, Q4KLN8, Q4QR06, Q4WZJ6, Q5R4I2, Q5R7K8, Q5R8L2, Q5SDR3, Q5ZM74, Q640D5, Q6CTZ8, Q6IV56, Q6IV57, Q6NRG0, Q75EN0, Q7T3E6, Q7ZX20, Q803C1, Q810I1, Q810I2, Q865W2, Q8IWZ4, Q8JIR0, Q8VC56, Q8WVD5

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
Ub:E2	“up-regulates activity”	RNF141	ubiquitination

Disease & clinical

Clinical variants and AI predictions

ClinVar

21 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	18
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1089 predictions. Top by Δscore:

Variant	Effect	Δscore
11:10519027:AACTT:A	donor_loss	1.0000
11:10519029:CTTAC:C	donor_loss	1.0000
11:10519030:TTACC:T	donor_loss	1.0000
11:10519031:TACCA:T	donor_loss	1.0000
11:10519032:A:C	donor_loss	1.0000
11:10519033:CCA:C	donor_gain	1.0000
11:10525179:A:AC	donor_gain	1.0000
11:10525179:ATG:A	donor_gain	1.0000
11:10525180:T:C	donor_gain	1.0000
11:10525194:T:A	donor_gain	1.0000
11:10530747:CCGTT:C	acceptor_gain	1.0000
11:10530748:CGTT:C	acceptor_gain	1.0000
11:10530748:CGTTC:C	acceptor_gain	1.0000
11:10530749:GTT:G	acceptor_gain	1.0000
11:10530750:TT:T	acceptor_gain	1.0000
11:10530750:TTC:T	acceptor_loss	1.0000
11:10530751:TCT:T	acceptor_loss	1.0000
11:10530752:C:CC	acceptor_gain	1.0000
11:10530752:CTGAA:C	acceptor_loss	1.0000
11:10530753:T:A	acceptor_loss	1.0000
11:10534014:A:AC	donor_gain	1.0000
11:10534015:C:CC	donor_gain	1.0000
11:10534015:CA:C	donor_gain	1.0000
11:10534015:CACAT:C	donor_gain	1.0000
11:10534038:T:A	donor_gain	1.0000
11:10519032:A:AC	donor_gain	0.9900
11:10519033:C:CC	donor_gain	0.9900
11:10519033:CCAT:C	donor_gain	0.9900
11:10519140:CC:C	acceptor_gain	0.9900
11:10519141:CC:C	acceptor_gain	0.9900

AlphaMissense

1523 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
11:10515038:A:G	C191R	0.999
11:10515047:A:G	C188R	0.999
11:10519046:C:G	C177S	0.999
11:10519047:A:G	C177R	0.999
11:10519047:A:T	C177S	0.999
11:10519056:A:G	C174R	0.999
11:10519057:A:C	F173L	0.999
11:10519057:A:T	F173L	0.999
11:10519059:A:G	F173L	0.999
11:10519071:A:G	C169R	0.999
11:10519104:A:G	C158R	0.999
11:10519113:A:G	C155R	0.999
11:10534046:A:G	F38S	0.999
11:10515005:A:G	W202R	0.998
11:10515005:A:T	W202R	0.998
11:10515036:A:C	C191W	0.998
11:10515037:C:G	C191S	0.998
11:10515037:C:T	C191Y	0.998
11:10515038:A:T	C191S	0.998
11:10515045:G:C	C188W	0.998
11:10515046:C:G	C188S	0.998
11:10515047:A:T	C188S	0.998
11:10519035:A:G	W181R	0.998
11:10519035:A:T	W181R	0.998
11:10519045:A:C	C177W	0.998
11:10519055:C:T	C174Y	0.998
11:10519058:A:G	F173S	0.998
11:10519063:G:C	H171Q	0.998
11:10519063:G:T	H171Q	0.998
11:10519069:A:C	C169W	0.998

dbSNP variants (sampled 300 via entrez): RS1000220279 (11:10527044 T>G), RS1000299471 (11:10536653 A>G), RS1000328860 (11:10533687 A>C), RS1000433200 (11:10516890 T>C), RS1000443536 (11:10533949 C>G,T), RS1000604064 (11:10535164 T>C), RS1000635151 (11:10534778 T>G), RS1000953541 (11:10539089 A>G), RS1001104453 (11:10520208 T>C), RS1001386110 (11:10539467 T>C), RS1001416331 (11:10515992 G>C), RS1001519402 (11:10541990 T>A,G), RS1001646843 (11:10542277 G>A), RS1001659105 (11:10527516 G>A), RS1001662610 (11:10522475 G>A,T)

Disease associations

OMIM: gene MIM:616641 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST009325_5	Parkinson’s disease or first degree relation to individual with Parkinson’s disease	2.000000e-09

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

30 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	increases abundance, affects methylation, affects cotreatment, decreases expression	2
dicrotophos	decreases expression	1
alpha phellandrene	increases expression	1
triphenyl phosphate	affects expression	1
bisphenol A	increases expression	1
arsenite	affects binding, increases reaction	1
cobaltous chloride	decreases expression	1
manganese chloride	affects cotreatment, decreases expression, increases abundance	1
di-n-butylphosphoric acid	affects expression	1
perfluorooctane sulfonic acid	decreases expression	1
monomethylarsonous acid	increases expression	1
K 7174	increases expression	1
Resveratrol	increases expression, affects cotreatment	1
Zoledronic Acid	decreases expression	1
Leflunomide	decreases expression	1
Acetaminophen	increases expression	1
Arsenic	affects cotreatment, decreases expression, increases abundance	1
Benzo(a)pyrene	increases methylation	1
Dexamethasone	increases expression	1
Diethylstilbestrol	decreases expression	1
Doxorubicin	decreases expression	1
Folic Acid	decreases expression	1
Manganese	affects cotreatment, decreases expression, increases abundance	1
Mercury	decreases expression	1
Plant Extracts	affects cotreatment, increases expression	1
Quercetin	increases expression	1
Valproic Acid	affects expression	1
Aflatoxin B1	decreases methylation	1
Antirheumatic Agents	decreases expression	1
Acrylamide	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.